SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
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IF 8.2
Zihan Wang,Xinru Wang,Ziqiang Li,Min Wang,Wenwen Fan,Chengmin Zha,Li Zhou,Xinzhong Zhang,Zongmao Chen,Fengjian Luo
Residual pesticides in agricultural environments, including soil and irrigation water, can be taken up by plants, and thus pose a potential risk to food safety. Although tolfenpyrad has been widely used in tea plantations, limited information is available on its root uptake and fate in tea plants ( Camellia sinensis L.). Exploring the mechanisms involved is crucial for understanding the migration and accumulation of tolfenpyrad in tea plants, particularly in the edible parts. In this study, root uptake of tolfenpyrad and its subsequent translocation, distribution, and metabolism in tea seedlings were investigated. The results indicated that the passive transport and apoplastic pathway dominated the root uptake of tolfenpyrad. After uptake, tolfenpyrad distributed predominantly in the cell walls (90.8–92.0 %) of roots, resulting in limited upward translocation in water-soluble fractions through transpirational pull, with translocation factor values far <1 (TF stem/root = 0.115–0.453 and TF leaf/stem = 0.039–0.184). Similar accumulation patterns were observed for the carboxylated metabolite PT-CA as well as hydroxylated metabolite PT-OH. Interestingly, the subcellular distribution of PT-CA in stems was much different from that of the parent tolfenpyrad: PT-CA mainly distributed in the stem cell walls (41.72 %) and cell organelles (56.18 %) at 3 h, then gradually transferred into the cell-soluble fractions (33.07 %) after 120 h. Results from the present study indicated limited upward translocation of tolfenpyrad with its main metabolites to leaves. This finding helps to alleviate concerns about environmental residual tolfenpyrad in tea consumption and provides valuable information for the safety evaluation of tolfenpyrad.
研究领域:
农业科学
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环境科学
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植物生理学
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DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF ORAL SCIENCES
·
2017
·Vol. 125
·期 5
·396-402
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IF 1.54
Fan Fan,Muhammad Ibrahim,Panpan Dai,Yixin Mao,Bing He,Gang Wu,Jianfeng Ma,Shengbin Huang
The aim of this study was to evaluate the effect of maleic acid (MA) on both the bond strength of fibre post to root dentine and smear layer removal after post space preparation. Sixty, single-canal premolars were endodontically treated and randomly assigned to four groups: group 1 [0.9% saline solution (control]); group 2 [2.5% sodium hypochlorite (NaOCl)]; group 3 [17% ethylenediaminetetraacetic acid (EDTA) followed by 2.5% NaOCl]; and group 4 (7% MA followed by 2.5% NaOCl). Self-adhesive resin cement was used to test the adhesion of a glass-fibre post to the root dentine through a micropush-out test. Scanning electron microscopy was performed to examine and score the treated specimens for smear layer removal, and stereomicroscopy was applied to investigate the failure modes of fibre posts. Maleic acid exhibited the highest mean bond-strength values in the apical regions among all the groups. Most failure modes (31.9%) were adhesive-type failures between the dentine and luting materials. Maleic acid performed statistically significantly better than the other groups regarding smear layer removal, especially in the apical region. Maleic acid is an effective irrigant that can remove the smear layer, open dentinal tubules, and act as a high-efficiency final irrigant in activation protocols.
研究领域:
牙科学
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材料科学
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DOI
PubMed
高影响因子
Frontiers in Plant Science
·
2024
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IF 4.1
Jiang Meng,Peng Mengwen,Li Yuxia,Li Guifang,Li Xiaobo,Zhuang Li
Introduction: Ferula is a traditional, edible, and important medicinal plant with high economic value. The distinction between edible and non-edible Ferula remains unclear.Methods: In this study, headspace solid-phase microextraction coupled to gas chromatography-mass spectrometry (HS-SPME/GC-MS) and ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) non-targeted metabolomics techniques were used to systematically and comprehensively analyse secondary metabolites in the leaves and roots of four species of Ferula, considering their edibility.Results: A total of 166 leaf volatile organic compounds (VOCs) and 1,079 root metabolites were identified. Additionally, 42 potential VOCs and 62 differential root metabolites were screened to distinguish between edible and non-edible Ferula. Twelve volatile metabolites were specific to F. feurlaeoides, and eight compounds were specific to the three edible Ferula species. The results showed that compounds containing sulphur, aldehydes, and ketones, which produce pungent odours, were the primary sources of the strong odour of Ferula. The root differential metabolites include 13 categories, among which the high concentration group is organic acids, amino acids, terpenoids and fatty acids. The bioactive metabolites and VOCs in the roots exhibited species-specific characteristics. VOCs with various odors were linked to the distribution of root metabolites in both edible and non-edible Ferula plants. The screened root markers may contribute to the formation of characteristic VOCs.Discussion: This study identified the difference in flavour between edible and non-edible Ferula plants and, for the first time, demonstrated the contribution of the efficacy of Ferula root to the unique flavour of the above-ground parts of Ferula. These results provide a theoretical basis for selecting Ferula for consumption and help evaluate the quality of different species of Ferula. Our findings may facilitate food processing and the further development of Ferula.
研究领域:
植物学
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食品科学
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药用植物研究
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DOI
PubMed
高影响因子
Sugar Tech
·
2025
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IF 2
He Wentao,Liu Naixin,Zhou Qin,Li Linxuan,Zhao Wenwen
There is a lack of systematic analysis and research on the special components of red beet and sugar beet, which are common beet varieties rich in a variety of beneficial chemicals. To investigate the differences in the composition of flavonoids and terpenoids in roots of red beet and sugar beet, poinsettia(red beet) and HD802(sugar beet) were detected and analysed in the present study by using the UPLC-MS/MS detection platform and the MWDB(Metware database), and orthogonal partial least squares discriminant analysis (OPLS-DA) was used to screen out the differential metabolites from the detected flavonoid metabolites and terpenoids metabolites. A total of 69 flavonoid metabolites from 6 classes and 48 terpenoids from 6 classes were detected in two kinds of beet roots. Analysis of variance (ANOVA) showed that the content of 5 flavonoid and 4 terpenoid differential metabolites was higher in red beet roots than in sugar beet roots, and the content of the remaining 25 flavonoid and 2 terpenoid differential metabolites was lower than that of sugar beet roots. According to the metabolite Log 2 FC, the components of the flavonoid metabolites with large difference ratio were mainly Polymethyl flavonoid derivatives, Iso-rhamnoside and Rhamnoside, Marigold glycoside, Methoxyl flavonoid derivatives, Diosmetin derivatives, Chrysoeriol and its derivatives, Luteolin derivatives, Homonetsin derivatives and Kaempferol derivatives. Significant differences in flavonoid metabolites and terpenoid metabolites between the two beet roots were found by non-targeted analysis of variance and can be further analysed as characteristic metabolites.
研究领域:
植物学
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代谢组学
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药用植物研究
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产品关联
DOI
高影响因子
FITOTERAPIA
·
2016
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IF 2.408
Wen-Jia Kang,Da-Hong Li,Tong Han,Lin Sun,Yan-Bin Fu,Chun-Mei Sai,Zhan-Lin Li,Hui-Ming Hua
Investigation of the roots of Flemingia philippinensis resulted in the isolation of two new chalcones , flemiphilippinones B ( 1 ) and C ( 2 ), and one new pterocarpoid, demethylwedelolactone-11-methyl ether ( 3 ), together with 12 known compounds ( 4 - 15 ). The antiproliferative activity against PC-3 cells was evaluated and most compounds showed cytotoxicity, among which, compound 2 exhibited GI 50 value of 14.12 μM. The antiproliferative activity of 2 against Bel-7402 and CaEs-17 cells was also measured, with GI 50 values of 1.91 and 2.58 μM, respectively. Intensive mechanism study showed that 2 caused cell-cycle arrest at S/G 2 phase and induced apoptosis in Bel-7402 cells through mitochondria-related pathway.
研究领域:
药用植物化学
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肿瘤生物学
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细胞信号通路研究
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产品关联
DOI
PubMed
高影响因子
Biomed Research International
·
2020
·Vol. 2020
·期 1
·5084057
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IF 2.276
Li Yang,Yiwei Fang,Ronghua Liu,Junwei He
Dendropanax dentiger root is a traditional medicinal plant in China and used to treat inflammatory diseases for centuries, but its phytochemical profiling and biological functions are still unknown. Thus, a rapid, efficient, and precise method based on ultra high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was applied to rapidly analyse the phytochemical profiling of D. dentiger with anti-inflammatory and antioxidant activities in vitro . As a result, a total of 78 chemical compositions, including 15 phenylpropanoids, 15 alkaloids, 14 flavonoids, 14 fatty acids, 7 phenols, 4 steroids, 4 cyclic peptides, 3 terpenoids, and 2 others, were identified or tentatively characterized in the roots of D . dentiger . Moreover, alkaloid and cyclic peptide were reported from D . dentiger for the first time. In addition, the ethanol crude extract of D. dentiger roots exhibited remarkable anti-inflammatory activity against cyclooxygenase- (COX-) 2 inhibitory and antioxidant activities in vitro . This study is the first to explore the phytochemical analysis and COX-2 inhibitory activity of D. dentiger . This study can provide important phytochemical profiles and biological functions for the application of D. dentiger roots as a new source of natural COX-2 inhibitors and antioxidants in pharmaceutical industry.
研究领域:
天然药物化学
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药用植物学
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炎症生物学
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自由基生物学
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药物代谢
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DOI
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2023
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IF 1.2
Jianjin GUO,Lu BAI,Chi-Tang HO,Sen GUO,Naisheng BAI
Fraxinus mandshurica is widely used in folk medicine to treat various diseases and has significant pharmacological effects. To make full use of this resource, its roots were studied. Twelve lignans were first obtained by natural product extraction, selective separation and identification, and were identified and quantified by high performance liquid chromatography-photodiode array (HPLC-DAD). The results of quantitative analysis showed that the highest lignin content in F. mandshurica roots was compound 9 with 102.02 mg/g, while the overall lignin content in Shanxi was low. The results of methodological validation showed that the RSD values of intra-day precision ranged from 0.35% to 1.38%, while the RSD values of inter-day precision were within 1.97%; the RSD values of reproducibility experiments ranged from 0.35% to 1.96%, while the RSD values of stability experiments were less than 2.97%; the spiked recovey of samples were within 102.84%, and the RSD values ranged from 0.39% to 1.77%, which fully confirmed the high precision and good reproducibility of the analytical method. In addition, fingerprinting and heatmap were established for 40 batches of samples from 8 origins. Next, multivariate analysis, including principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA), was performed to investigate the differences in lignin content in the roots of F. mandshurica from different origins, and four lignins that could be used to distinguish F. mandshurica from different origins were screened. Finally, 12 lignans were evaluated for enzyme activity inhibition with α-glucosidase, tyrosinase and acetylcholinesterase, and the results showed that all 12 lignans exhibited enzyme activity inhibition to different degrees, which have potential applications in anti-diabetic, whitening and light spot and anti-Alzheimer's disease, and become a new source of natural enzyme activity inhibitors. This study established an identification and evaluation method applicable to plants of different origins, which provides a certain theoretical basis for the comprehensive exploitation of F. mandshurica .
研究领域:
药用植物学
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天然产物化学
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生物活性研究
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产品关联
DOI
高影响因子
Nature Chemical Biology
·
2024
·
IF 12.9
Wang Shiwen,Liu Min,Hu Dongping,Dong Zhicheng,Zhao Zhong
Superoxide anion is thought to be a natural by-product with strong oxidizing ability in all living organisms and was recently found to accumulate in plant meristems to maintain stem cells in the shoot and undifferentiated meristematic cells in the root. Here we show that the DNA demethylase repressor of silencing 1 (ROS1) is one of the direct targets of superoxide in stem cells. The Fe–S clusters in ROS1 are oxidized by superoxide to activate its DNA glycosylase/lyase activity. We demonstrate that superoxide extensively participates in the establishment of active DNA demethylation in the Arabidopsis genome and that ARABIDOPSIS RESPONSE REGULATOR 12 acts downstream of ROS1-mediated superoxide signaling to maintain stem cell fate. Our results provide a mechanistic framework for superoxide control of the stem cell niche and demonstrate how redox and DNA demethylation interact to define stem cell fate in plants.
研究领域:
植物生物学
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表观遗传学
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干细胞生物学
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Tingting Liu,Jian Luo,Shangke Liu,Tongtong Li,Haolin Li,Lingyan Zhang,Wei Mu,Nan Zou
Neonicotinoid insecticides have been widely used due to their excellent systemic activity and high insecticidal activity, but the problems of low utilization rate and environmental risk have attracted widespread attention. Controlled-release technology is an approach to realize the efficient utilization of pesticides and reduce environmental pressure. In this study, clothianidin (CLO) controlled-release granules ( [email protected] TA (tannic acid)/Fe (III)) were prepared with TA/Fe (III) coordination chelate as the coating material. These granules exhibited the core-shell structure with 500–1200 µm of particle size, and had obvious release performance and hydrolysis behavior of coating materials. Pot experiments by root application showed that the [email protected] /Fe (III) showed balanced and lasting control efficacy to broad bean aphids. The plants have a stronger capacity for absorption and enrichment and a higher utilization rate of CLO for [email protected] /Fe (III), than those for 10% suspension concentrate (SC). One of the hydrolysates of coating materials, TA, a polyphenolic antioxidant, could improve the bioaccumulation amount and alleviating the oxidative stress response of CLO in plants. Our study illustrates that the controlled-release granules base on TA have efficient controlled-release properties and free radical scavenging performance that may eventually be used as pesticide carriers and antioxidants in the field of plant protection.
研究领域:
农业科学
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农药科学
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植物保护
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环境科学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL RESEARCH
·
2025
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IF 7.7
Wanyue Zhang,Zhihao Pang,Wanning Zheng,Sue E. Hartley,Zulin Zhang,Hongyun Peng,Yongchao Liang
Excessive accumulation of heavy metals such as trivalent chromium [Cr(Ⅲ)] threatens environmental sustainability and crop health. While silicon fertilizers are known to mitigate Cr(III) toxicity in plants, and silica nanoparticles (SiO 2 NPs) show promise for heavy metal stress alleviation, their comparative efficacy and underlying mechanisms remain unexplored. In this study, we compared the effects of SiO 2 NPs and sodium silicate (Na 2 SiO 3 ) in alleviating Cr(Ⅲ) stress in rice and elucidated the mechanisms behind. Results showed that SiO 2 NPs reduced Cr(Ⅲ) accumulation more effectively than Na 2 SiO 3 in rice shoots, roots and xylem sap. Trisodium-8-hydroxy-1,3,6- pyrenetrisulfonic (PTS) tracer analysis confirmed that SiO 2 NPs more significantly inhibited Cr(Ⅲ) apoplastic transport. Consequently, SiO 2 NPs better alleviated Cr(Ⅲ)-induced growth inhibition and malondialdehyde (MDA) accumulation. Both SiO 2 NPs and Na 2 SiO 3 exhibited tissue-specific regulation of hydrogen peroxide (H 2 O 2 ) detoxification: SiO 2 NPs more effectively reduced H 2 O 2 accumulation in roots and increased catalase (CAT), ascorbate peroxidase (APX), and peroxidase (POD) activities in shoots, while Na 2 SiO 3 showed the opposite pattern. The mechanistic studies demonstrated that the root-specific distribution of SiO 2 NPs facilitated their stronger physical barrier formation in roots. Furthermore, SiO 2 NPs outperformed Na 2 SiO 3 in reinforcing endodermal apoplastic barriers by promoting lignin accumulation and upregulating lignin biosynthesis-related genes ( OsCCR , OsC4H , Os4CL , OsCAD , OsPAL ), while also promoting earlier initiation of endodermal suberin deposition near root tips. Overall, our study indicates that SiO 2 NPs more effectively reinforced apoplastic barriers, restricted the apoplastic transport of Cr(Ⅲ) and consequently alleviated Cr(Ⅲ)-induced stress in rice.
研究领域:
植物科学
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纳米技术
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环境毒理学
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农业化学
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生物技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Jingyuan Wang,Rong Li,Zongyuan Zhao,Meiqing Zhu,Yi Wang
Prothioconazole (PTC) has been widely utilized for plant fungal disease control, but its metabolite prothioconazole-desthio (PTC-d) exhibits reproductive toxicity. In the present study, carbon quantum dot (CQD)-modified fluorescent double-hollow shelled mesoporous silica nanoparticles (FL-MSNs) loaded with PTC, referred to as PTC@FL-MSNs, were constructed with an average size of 369 nm and a loading capacity of 28.1 wt %, which could increase the antifungal efficiency of PTC. In addition, upright fluorescence microscope and UPLC-MS/MS studies showed that PTC@FL-MSNs could be effectively transported via root uptake and foliar spray in soybean plants. Compared to a 30% PTC dispersible oil suspension agent, the PTC@FL-MSN treatment group showed higher concentrations (leaves: 0.50 > 0.48 mg/kg), longer half-lives for degradation (leaves: 3.62 > 3.21 d; roots: 3.39 > 2.82 d), and fewer metabolites. These findings suggest that sustained pesticide release and toxicity reduction are potential applications for PTC nanofungicide delivery technology.
研究领域:
农学
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纳米技术
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植物病理学
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环境科学
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产品关联
DOI
PubMed
高影响因子
iScience
·
2022
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IF 6.107
Fanyang Lv,Yuhua Zhan,Wei Lu,Xiubin Ke,Yahui Shao,Yiyuan Ma,Juan Zheng,Zhimin Yang,Shanshan Jiang,Liguo Shang,Yao Ma,Lei Cheng,Claudine Elmerich,Yongliang Yan,Min Lin
Summary Bacteria of the genus Pseudomonas consume preferred carbon substrates in nearly reverse order to that of enterobacteria, and this process is controlled by RNA-binding translational repressors and regulatory ncRNA antagonists. However, their roles in microbe-plant interactions and the underlying mechanisms remain uncertain. Here we show that root-associated diazotrophic Pseudomonas stutzeri A1501 preferentially catabolizes succinate, followed by the less favorable substrate citrate, and ultimately glucose. Furthermore, the Hfq/Crc/CrcZY regulatory system orchestrates this preference and contributes to optimal nitrogenase activity and efficient root colonization. Hfq has a central role in this regulatory network through different mechanisms of action, including repressing the translation of substrate-specific catabolic genes, activating the nitrogenase gene nifH posttranscriptionally, and exerting a positive effect on the transcription of an exopolysaccharide gene cluster. Our results illustrate an Hfq-mediated mechanism linking carbon metabolism to nitrogen fixation and root colonization, which may confer rhizobacteria competitive advantages in rhizosphere environments.
研究领域:
微生物学
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植物科学
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分子生物学
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DOI
PubMed
高影响因子
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