Scientific Reports
·
2019
·Vol. 9
·期 1
·1-5
·
IF 4.011
Mei Jianfeng,Chen Xiang,Liu Jianghua,Yi Yu,Zhang Yanlu,Ying Guoqing
Genistein is known to have multiple biological activities and has great potential for use as a preventative medicine and in disease treatment. Genistein can be extracted from plants, but also can be obtained from its glycoside form, sophoricoside, which is more abundant in some plants. Biotransformation by unpurified microbial enzymes has the advantage of low cost and is a preferred method for production of natural compounds. This study isolated a strain of Rhizopus oryzae that could produce β -glucosidase, which efficiently hydrolyzes sophoricoside into genistein, from an enrichment culture of the dried fruits of Sophora japonica . After the composition of enzyme-producing medium and biotransformation conditions were optimized, a genistein yield of 85.6% was obtained after 24 h in a shake-flask biotransformation at pH 7.0 using an initial substrate concentration of 1 g/L. The developed process provides an alternative method for production of genistein, and would be suitable for scale-up production in the pharmaceutical industry.
研究领域:
微生物学
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药理学
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生物技术
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产品关联
DOI
PubMed
高影响因子
BIOLOGICAL CONTROL
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2019
·
IF 2.607
Xiaoyu Zhang,Zhenfeng Gao,Xinxian Zhang,Wenbin Bai,Lixin Zhang,Hongbin Pei,Yongjie Zhang
Post-harvest diseases of raspberries affect their storage and subsequently the ability to provide consumers with fresh products. The antifungal activity of microbial volatile organic compounds (MVOCs) against both pre and postharvest diseases has previously been demonstrated, but the control of raspberry postharvest diseases caused by Botrytis cinerea and Rhizopus stolonifer using MVOCs has not been extensively studied. We have thus investigated the effects of Bacillus siamensis G-3 volatile organic compounds (VOCs) on controlling the diseases caused by B. cinerea and R. stolonifer . Bioassays of the VOCs of B. siamensis G-3 exhibited significant control effects (89.4%) on the two pathogens. The VOCs composition was studied with solid phase micro extraction and GC/mass spectrometry (SPME-GC-MS), and 22 VOCs, different from the controls, were identified. Among these compounds, only 2, 6-di- tert -butyl-4-methylphenol (BHT), and 2,4-di- tert -butylphenol (2,4-DTBP) had significant control effects on the pathogens (* P < 0.05), 52.38 and 93.33%, respectively, compared with the controls in vivo . With the 2,4-DTBP treatments, the raspberries could be stored for 20 d at 0 °C, with the disease rate maintained at ∼10%. In addition, we have reported for the first time, that 2,4-DTBP may be a better preservative than BHT and could be used as a bio-fumigant for controlling raspberry postharvest diseases caused by B. cinerea and R. stolonifer in vivo .
研究领域:
植物病理学
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食品科学
✓ 已验证
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Han Wu,Hao-Nan Liu,Ai-Min Ma,Jian-Zhong Zhou,Xiu-Dong Xia
In this study, Lactobacillus plantarum strains, namely 70810, B1-6, and ZR were used to confirm the synergetic effects of co-inoculation with Lactobacillus and Rhizopus oryzae on whole-oat solid state fermentation (SSF). The results indicated that number of viable bacterial cells increased more quickly (10.72–11.23-fold higher) during co-inoculated SSF than that during pure Lactobacillus fermentation. Fungal ergosterol content reached slightly less during co-inoculation (137.04–155.12 mg/kg) than that during R. oryzae fermentation (193.64 mg/kg). However, compared to R. oryzae , R. oryzae combined with Lactobacillus produced more obvious hydrolysis of macromolecules and accelerated more water transformation from confined to free form. As for co-inoculated SSF of oat, the reducing sugar content reached the highest at 41.09–47.42 g/100g and then dropped, and free amino acid nitrogen content varied gradually until 40.66–41.67 mg/100g. Fermented oats (FOs) had higher total phenolic content than unfermented oat (UO) (2.56–2.72-fold, FO vs . UO), and their DPPH radical scavenging activity, ferric reducing capacity, and hydroxyl scavenging activity were increased 2.04-, 2.10-, and 1.99-fold at maximum (FO vs . UO), respectively. The Lactobacillus showed strain dependence during co-inoculated SSF, among which B1-6 displayed the best to enhance oat's overall quality.
研究领域:
食品科学
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微生物学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2022
·
IF 5.911
Wenguang Fan,Baoyu Li,Nana Du,Tian Hui,Yingying Cao,Xin Li,Haiwei Ren
3-phenyllactic acid (PLA) has broad anti-fungal activity, however, target sites of PLA on fungal cells and its anti-fungal mechanism of action have been poorly studied. In this study, we explored the inhibition mechanism of Rhizopus oryzae ( R. oryzae ) on rotten lily bulbs by PLA. The minimum inhibitory concentration value of PLA against R. oryzae was 8 mg/mL. We observed the ultrastructure of R. oryzae by scanning electron microscopy and transmission electron microscopy which indicated that PLA did not damage the cell membrane, but destroyed the mitochondria and other organelles. Tandem mass tag proteomes showed that PLA significantly down-regulated ( P < 0.05) the expression of hexokinase (HK), phosphofructokinase (PFK), a-ketoglutarate dehydrogenase (a-KGDH), adenylate kinase (ADK1), Cytochrome C oxidase and NADH dehydrogenase, and up-regulated ( P < 0.05) the expression of mitochondrial ADP/ATP carrier proteins (AAC) and subunit IV (CCIO IV) in glycolysis, tricarboxylic acid cycle or oxidative phosphorylation metabolism. Following these findings, down-regulated HK and a-KGDH activity of aforementioned pathways was shown by enzyme activity assay, and regulated gene expression of ADK1, AAC, CCIO IV and NADH dehydrogenase was further confirmed by real-time quantitative PCR. Central carbon metabolomics showed that citric acid, cis-Aconitic acid, isocitric acid, alpha-Ketoglutaric acid, succinate, fumarate and malic acid of the tricarboxylic acid cycle metabolites were significantly down-regulated ( P < 0.05), and ATP production by oxidative phosphorylation was also significantly reduced ( P = 0.02), resulting in insufficient energy production. Thus, ROS levels increased by 141% of the control values and cytochrome C was released, resulting in gradual cell apoptosis. All data indicated that energy metabolism was the target of PLA against R. oryzae . This was the first study to show that energy metabolism could be the target of PLA against R. oryzae , which could provide a theoretical basis to study the mechanism of fungal inhibition.
研究领域:
微生物学
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真菌学
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生物化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 5
·1046
·
IF 4.2
Xiurong Ju,Tao Chen,Yutao Ding,Dan Yu,Jingyu Zhang,Ruyuan Zhang,Yang Zhang,Xinyu Wang,Tao Xu,Jiayou Li
TheHibiscus manihotL. (HML) Medic, an edible hibiscus of the Malvaceae family, is abundant with flavonoids. The study investigated howRhizopus-arrhizus-31-assisted pretreatment affects the extraction and bioactivity of flavonoids from HML. The fiber structure of the fermented flavonoid sample (RFF) appears looser, more porous, and more disordered than the unfermented flavonoid sample (RUF). RFF demonstrates milder conditions and yields higher extraction rates. According to the Box–Behnken response surface optimization experiment, the optimal conditions for RFF include a material–liquid ratio of 1:41 g/mL, a 2 h extraction time, a 57% ethanol concentration, and an extraction temperature of 800 °C, resulting in a 3.69% extraction yield, which is 39.25% higher than that of RUF. Additionally, RFF exhibits greater activity than RUF in the radical-scavenging system. The IC50 values for DPPH, OH, and ABTS radicals are 83.43 μg/mL and 82.62 μg/mL, 208.38 μg/mL and 175.99 μg/mL, and 108.59 μg/mL and 75.39 μg/mL for RUF and RFF, respectively. UPLC-QTOF-MS analysis of the active components in the HML flavonoid sample revealed significant differences in the chromatograms of RUF and RFF, indicating that biofermentation led to substantial changes in composition and content from HML.
研究领域:
药用植物学
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生物技术
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食品科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2022
·
IF 9.231
Duqin Zhang,Yanjun Ye,Bin Tan
The effects of solid-state fermentation (SSF) with Lactiplantibacillus plantarum , Saccharomyces cerevisiae , Rhizopus oryzae , Aspergillus oryzae, and Neurospora sitophila were determined on the bioactive compound content and grain microstructure of brown rice (BR). After SSF, the β-glucan, arabinoxylans, γ-oryzanol, thiamine, riboflavin, phenolic, and flavonoid contents increased by 147, 11.2, 30.5, 16.9, 21.1, 76%, and 49.6%, respectively, indicating a marked increase in bioactive compound content. In addition, the water-soluble dietary fiber and arabinoxylan contents, and free phenolic and flavonoid contents significantly increased ( p < 0.05 ). These changes were consistent with the microstructural changes observed after SSF, i.e., the outer cortex was rough, cracked, porous and separated from the starch endosperm, which was also cracked and porous; this should increase the dietary bioavailability of the bioactive compounds. SSF, especially with A. oryzae and Lb. plantarum , greatly enhanced the bioactive compound content in BR and has great potential in BR processing.
研究领域:
食品科学
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微生物学
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营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOCONTROL
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2025
·
IF 2.7
Wang Jian-Yu,Mao Min-Xin,Xu Chun-Yan,Zou Man,Bao Wen-Ting,Zhou Yi-Ning,Hu Shun-Qing,Jiao Ke-Xin,Lu Qing-Kun,Zhao Peng,Zhang Kun-Peng,Chen Yi-Lun,Zhang Qian
The soft rot of sweet cherries caused by Rhizopus stolonifer ( R. stolonifer ) results in significant economic losses during storage and transportation. This study demonstrated that volatile organaic compounds (VOCs) derived from the fermentation broth of Bacillus velezensis ( B. velezensis ) Q-84 significantly inhibited the growth of R. stolonifer by 54.43%. Scanning electron microscope (SEM) micrographs of VOCs-treated fungi revealed antifungal activity, evidenced by the breakage and deformation of the mycelium. Through headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC/MS) analysis, ten VOCs were identified in the fermentation broth of B. velezensis . Notably, 5-methyl-2-heptanone, 2,5-dimethylpyrazine, 2-decanone, and benzaldehyde exhibited significant antifungal activity in vitro . Furthermore, in vivo assays demonstrated the efficacy of both individual and combined VOCs in managing soft rot diseases in cherries. These treatments also resulted in increased levels of total polyphenolic content (TPC) and total flavonoid content (TFC), as well as a significant enhancement in the activities of defense-related enzymes such as peroxidase (POD), polyphenol oxidase (PPO), phenylalanine ammonia-lyase (PAL), and chitinase (CHI). Our results further our understanding of B. velezensis antifungal metabolites on the management of R. stolonifer and may help in finding less toxic compounds to control soft rot on sweet cherries.
研究领域:
植物病理学
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微生物学
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农业科学
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食品科学
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生物化学
✓ 已验证
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2024
·
IF 6
Zhongai Chen,Chen Chen,Run Yang,Hanshi Jin,Beining Liu,Zhirong Wang,Yumei Tan,Qiaoli Xue,Yongjin Hu
Sufu, a traditional fermented soybean product, known for its cheese-like texture, has not been extensively studied regarding the mechanisms responsible for the formation of this unique texture in sufu gels. In order to explore this phenomenon, an analysis was conducted on the texture, physicochemical properties, and microstructure of sufu gels fermented by mucor racemosus at different intervals. The findings indicated no significant difference in water holding capacity (WHC) of sufu gel at 0 h, 24 h, and 48 h, but a notable increase in WHC was observed at 72 h and 96 h ( p<0.05 ). The hardness of sufu gel at 72 h and 96 h was found to be low, with values of 4.8± 0.56 N and 4.99± 0.54 N, respectively, indicating a soft texture of sufu. The peak of protease activity at 48 h was 38.19 U/g. Following fermentation for 24 h and 48 h, the secondary structure of sufu gel was predominantly composed of β-sheet, while α-helix was the primary secondary structure at 72 h and 96 h. Analysis using the scanning electron microscope (SEM) and confocal laser scanning microscopy (CLSM) analysis revealed the network-like structures present in sufu gel. These findings offer valuable insights for enhancing gel properties and sensory characteristics.
研究领域:
食品科学
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微生物学
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生物技术
✓ 已验证
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Yong Wang,Ziyuan Wang,Huifen Yu,Haidong Teng,Jianping Wu,Jiaqi Xu,Lirong Yang
Lipase from Rhizopus oryzae (ROL) exhibits remarkable sn-1,3 stereoselectivity and catalytic activity, but its poor thermostability limits its applications in the production of 1,3-dioleoyl-2-palmitoyl glycerol (OPO, a high-quality substitute for human milk fat). In this work, a semirational method was proposed to engineer the thermostability and catalytic activity of 4M (ROL mutant in our previous study). First, a computer-aided design is performed using 4M as a template, and N-glycosylation mutants are then recombinantly expressed and screened in Pichia pastoris, the optimal mutant N227 exhibited a half-life of 298.8 h at 45 °C, which is 7.23-folds longer than that of 4M. Its catalytic activity also reached 1043.80 ± 61.98 U/mg, representing a 29.2% increase compared to 4M (808.02 ± 47.02 U/mg). Molecular dynamics simulations of N227 suggested that the introduction of glycan enhanced the protein rigidity, while the strong hydrogen bonds formed between the glycan and the protein stabilized the lipase structure, thereby improving its thermostability. The acidolysis reaction between oleic acid (OA) and glycerol tripalmitate (PPP) was successfully carried out using immobilized N227, achieving a molar conversion rate of 90.2% for PPP. This engineering strategy guides the modification of lipases, while the glycomutants obtained in this study have potential applications in the biosynthesis of OPO.
研究领域:
生物技术
·
酶学
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蛋白质工程
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生物化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Changding Gao,Mei Yang,Yihan Liu,Yuhan Wang,Ligong Zhai,Jinlong Zhao,Yijun Liu,Baoshi Wang
The insoluble dietary fiber (IDF) of pomegranate peel residue was treated by mixed solid-state fermentation (SSF) of Aspergillus niger and Rhizopus oryzae. The content of bound polyphenols (BP) reached a maximum of 26.50 mg GAE/g DW after 5 days of fermentation, which was 3.22-fold higher than that of the unfermented group. The BP release during fermentation was strongly correlated with the activities of CMCase, xylanase and β-glucosidase. A total of 35 polyphenolic compounds and catabolic metabolites were identified, and potential biotransformation pathways were proposed. Structural characterization of IDF before and after fermentation via SEM, XRD, and TGA revealed that the degradation of cellulose and disruption of hemicellulose and hemicellulose–lignin complexes played a key role in BP release. Moreover, the postfermentation BP in vitro showed enhanced antioxidant capacity and pronounced inhibition of α-amylase/α-glucosidase. Collectively, the mixed SSF significantly promote the BP release, facilitating pomegranate peel valorization.
研究领域:
食品科学
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微生物学
·
生物化学
·
生物技术
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营养学
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植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Hong Yang,Mingming Yu,Hao Shi,Fei Wang,He Liu,Xun Li
The limited methanol tolerance and glycerol adsorption tendency of whole-cell catalysts are the main factors contributing to the extended catalytic cycle and limited catalyst reusability in biodiesel preparation. In this study, a 7.5 % methanol-tolerant Rhizopus stolonifera mutant (MTS2) was developed through the atmospheric and room temperature plasma (ARTP), exhibiting a 1.5-fold increase in methanol tolerance compared with the wild type strain (WT-RS), along with a 93.5 % increase in lipases activity. Polyurethane foam PF3 with an average pore size of 0.15 mm and 90 A rigidity is optimal for MTS2 immobilization (MTS2 @PF3), enabling its application in continuous processing for industrial applications. Utilizing soybean oil as a substrate, the catalyst MTS2 @PF3 produced 95.5 % of fatty acid methyl esters (FAME) in 36 hours under optimal conditions (30°C, 180 r/min, catalyst dosage of 15 % g/g oil, 30 % water content, and methanol addition in Scheme 3), achieving over a 50 % reduction in reaction time compared with WT-RS@PF3. To address limitations in catalyst reusability due to glycerol adsorption, various modifiers, including rosin-based modifier (RM), amino silicone oil (ASO), graphene (GRA), betaine and Tween 80 were evaluated. The glycerol adsorption rates experienced substantial reductions (39.3 % with RM and 85.6 % with betaine), and the catalysts maintained above 60 % FAME yield even after 8 reaction cycles. The combination of enhanced methanol tolerance, lipase activity, and reduced glycerol adsorption contribute to enhanced catalytic efficiency and reusability, which can reduce production costs for biodiesel manufacturing. The composite of these attributes makes MTS2 @RM1-PF3 and Betaine-MTS2 @PF3 as promising candidate for industrial biodiesel production, addressing previous limitations in catalyst performance and sustainability. The developed immobilized whole-cell catalysts exhibit promising potential for more sustainable and cost-effective processes in biodiesel manufacturing.
研究领域:
生物技术
·
生物化工
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可再生能源
·
催化
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Jian Guo,Zhiping He,Chao Ma,Wanting Li,Jiaoyu Wang,Fucheng Lin,Xingquan Liu,Ling Li
Few reports are on the application of cold plasma (CP) for mold and mycotoxin control in grain. The aim of this study was to evaluate the viability of CP for mycotoxin and mold control in rice grain. Rice grains artificially contaminated with molds or mycotoxins were subjected to CP treatment. The microbial activities of Aspergillus niger , Rhizopus oryzae , Penicillium verrucosum and Fusarium graminearum were significantly inhibited by CP treatment. The maximal reduction of DON and OTA were 61.25 % and 55.64 %, respectively. The electrical conductivity and malondialdehyde content in rice grain increased at most by 30.14 % and 103.27 %, respectively. The seed germination reduced significantly when treatment time reached 8 min. The major nutrients of rice grain were not affected except for prolamine, which was generally consistent with the results of electron microscopy and Fourier-transform infrared analysis. Cold plasma is a promising method for mold and mycotoxin control in rice grain.
研究领域:
食品科学
·
微生物学
·
等离子体物理
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毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）