JOURNAL OF SEPARATION SCIENCE
·
2014
·Vol. 37
·期 18
·2490-2498
·
IF 2.594
Xiaoxue Wu,Jiao He,Huarong Xu,Kaishun Bi,Qing Li
A novel and improved method for the quality assessment of Cinnamomi Ramulus was developed and completely validated. The method was established using fingerprint technology and simultaneous quantitative determination of six main marker compounds including coumarin, cinnamic alcohol, cinnamic acid, 2-methoxy cinnamic acid, cinnamaldehyde, and 2-methoxy cinnamaldehyde in the herbal medicine for the first time. A newly developed high-performance thin-layer chromatography method, which achieved simultaneous definition of five marker components by comparing the colors and retardation factor values of the bands in high-performance thin-layer chromatography, was first used for the authentication of Cinnamomi Ramulus. The fingerprints of 26 batches of herbal samples from different regions of China showed very similar chromatographic patterns that were evaluated by similarity analysis and hierarchical clustering analysis. In addition, six marker compounds were simultaneously determined using single standard to determine multiple components by the relative response factors. Compared with the external standard method, the new quantitative method was validated to determine multiple compounds in 26 batches of Cinnamomi Ramulus samples. All results demonstrated that the simple and rapid method could be effectively utilized for the quality control of Cinnamomi Ramulus.
研究领域:
生药学
·
分析化学
·
中药
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2019
·
IF 4.916
Yu-Ning Hu,Di Chen,Tian-Yi Zhang,Jun Ding,Yu-Qi Feng
Derivatization is frequently used in liquid chromatography–mass spectrometry (LC-MS) to improve the ionization characteristics of analytes, and facilitate their structural elucidation and quantification. However, the off-line derivatization process usually requires more analysis time. Therefore, the present work develops a novel LC/post-column derivatization (PCD)/MS (LC-PCD-MS) method for rapid detection of aldehydes in complex samples. The PCD method is based on a bisulfite addition reaction with aldehydes that can be easily conducted by mixing the LC elute with an (NH 4 ) 2 SO 3 solution via a tee. The resulting derivatives readily produce diagnostic HSO 3 − ions under collision induced dissociation (CID) in the negative ion mode of MS, even at an extremely low CID energy such as 1 eV, which can significantly improve the detection selectivity of aldehydes and reduce the occurrence of false positives. The limits of detection (LODs) of hexanal and heptanal were 3 nM and 2 nM, respectively. Moreover, good linearity in the concentration range of 0.01–1 μM for hexanal and heptanal was obtained with the determination coefficient (R 2 ) being greater than 0.996 and the intra- and inter-day relative standard deviations (RSDs) were lower than 13.5% and 17.9%, respectively, indicating the acceptable reproducibility of the method. The LC-PCD-MS method was successfully applied for the rapid quantification of aldehydes in human urine and solvent extracts of traditional Chinese medicines (TCMs). Furthermore, a precursor ion scan strategy based on the diagnostic HSO 3 − ion was also developed and applied for the preliminary screening of aldehydes in complex samples of cinnamon and lemongrass.
研究领域:
分析化学
·
医学检验
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yuxin Zhang,Jiawei Huangfu,Bin Rong,Xiongfei Du,Yang Hu,Xu Zeng,Kaikai Chen,Changkun Ding,Wenping Hu,Kuo Yuan,Qinglin Huang
Aerogel, characterized by ultra-low density and scalable preparation, is extensively utilized in various applications, such as catalysis, adsorption/separation, and thermal insulation. However, the trade-off between durability and flexibility for aerogels remains a fundamental challenge, limiting their further development in harsh and complicated conditions. Herein, we developed a confined Ostwald ripening approach to construct the first example of 3D polytetrafluoroethylene (PTFE) aerogel without additional binders. This aerogel possessed an ultra-low density of 0.042 g·cm −3 , vertically aligned channels, and complex superhydrophobic surface structure, which realized the highest oil flux (5.09 × 10 5 L·m −2 ·h −1 ) in the ultra-fast oil/water separation so far. This excellent performance could be maintained in strong acid, alkaline, corrosive organic, or complicated emulsion solutions. Moreover, this pure PTFE skeleton exhibited a high Young's modulus of 0.08 MPa, an elastic modulus of 5.24 × 10 −4 MPa, and excellent flexibility. Following, the 3D PTFE aerogel was employed in Pickering emulsion bi-phase photocatalysis for highly efficient photocatalyst cycling and product separation. This work not only addresses the inherent poor processability of PTFE but also breaks the trade-off between durability and flexibility for aerogels, which greatly boosts its development in long-term practical applications under harsh and complicated conditions.
研究领域:
材料科学
·
环境工程
·
化学工程
·
纳米技术
·
催化化学
·
界面科学
✓ 已验证
产品关联
DOI
高影响因子
Foodborne Pathogens and Disease
·
2024
·
IF 1.9
Ziying Lu,Tong Xing,Zepeng Zhao,Zefeng Li,Dongping Hou,Zhuolin Ma,Siyi Chen,Yuheng Yang,Shaoting Li,Hongmei Zhang
Trans-cinnamaldehyde (TC), a typical plant-derived compound, has been widely used in the control of foodborne pathogen contamination. Nevertheless, the risk associated with the occurrence of viable but nonculturable (VBNC) bacteria induced by TC remains unclear. The results of this study showed that Salmonella Enteritidis (S. Enteritidis) entered the VBNC state after being induced by TC at a minimum inhibitory concentration of 312.5 μg/mL and survived for at least 22 days under TC treatment. Enhanced resistance was found against heat treatment (75°C, 30 s), antibiotics (i.e., ampicillin, ceftriaxone sodium, chloramphenicol), and hydrogen peroxide (3%) in VBNC S. Enteritidis. A synergistic effect against VBNC S. Enteritidis occurred when TC was combined with acid treatment, including lactic acid and acetic acid (pH = 3.5). VBNC and resuscitated S. Enteritidis by sodium pyruvate treatment (100 mM) were found to retain the infectious ability to Caco-2 cells. Relative expression levels of the stress-related genes relA, spoT, ppx, lon, katG, sodA, dnaK, and grpE were upregulated in VBNC S. Enteritidis. Accumulation of reactive oxygen species (ROS) and protein aggregates was observed in VBNC cells. Besides, the resuscitation of VBNC cells was accompanied with clearance of ROS and protein aggregates. In summary, this study presents a comprehensive characterization of stress tolerance and resuscitation of VBNC S. Enteritidis induced by cinnamaldehyde, and the results provide useful information for the development of effective control strategy against VBNC pathogenic bacteria in food production.
研究领域:
食品安全
·
微生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
·
2020
·
IF 1.101
Jinhao Zhao,Zhongyan Wang,Zhongshan Li,Jiayu Shi,Ling Meng,Guorong Wang,Jingli Cheng,Yongjun Du
Aphids are among the most destructive phytophagous pests of host plants, because of their rapid reproduction, parthenogenesis, extensive crop damage, and the transmission of many plant viruses. Since lady beetles are important predatory natural enemies of aphids, developing lady beetle attractants to increase their field abundance is vital for aphid control. Floral volatiles and other semiochemicals are reportedly attractive to lady beetles. In this research, a total of 58 floral volatiles were tested by Y-tube olfactometer assays, among which 29 were highly attractive to both Harmonia axyridis (Coleoptera: Coccinellidae) and Coccinella septempunctata (Coleoptera: Coccinellidae). Meanwhile, the results of wind tunnel trials showed that only isoamyl acetate, α -humulene, trans -3-hexen-1-ol, methyl salicylate, and β -pinene lure these two species. Thereafter, 15 semiochemicals from pests, natural enemies, and pest-infested crops were mixed with the selected floral volatiles, to determine optimum formulations for attracting lady beetles through wind tunnels and further field trials. Eventually, formulas (1) α -humulene: β -pinene: methyl salicylate: trans -3-hexen-1-ol = 1:3:3:10; (5) α -humulene: β -pinene: methyl salicylate:1-nonanal = 1:3:3:10; (8) α -humulene: β -pinene: methyl salicylate: indole = 1:3:3:10 ( 1 , 5 , and 8 are labels used for the various formulations in field trials) were successfully verified to be attractive to lady beetles in both pumpkin and wheat fields. These formulations will be of great significance in developing integrated pest management strategies for aphid control.
研究领域:
昆虫学
·
农业科学
·
害虫管理
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Pharmacology
·
2021
·
IF 5.811
Liao Wenting,Jin Qiwen,Liu Junning,Ruan Yiling,Li Xinran,Shen Yueyue,Zhang Zhicheng,Wang Yong,Wu Shengming,Zhang Junying,Kang Lifeng,Wu Chunyong
Acute liver failure (ALF) is a serious clinical disorder with high fatality rates. Mahuang decoction (MHD), a well-known traditional Chinese medicine, has multiple pharmacological effects, such as anti-inflammation, anti-allergy, anti-asthma, and anti-hyperglycemia. In this study, we investigated the protective effect of MHD against ALF. In the lipopolysaccharide and D-galactosamine (LPS/D-GalN)-induced ALF mouse model, the elevated activities of the serum alanine and aspartate transaminases as well as the liver pathological damage were markedly alleviated by MHD. Subsequently, a metabolomics study based on the ultrahigh performance liquid chromatograph coupled with Q Exactive Orbitrap mass spectrometry was carried to clarify the therapeutic mechanisms of MHD against ALF. A total of 36 metabolites contributing to LPS/D-GalN-induced ALF were identified in the serum samples, among which the abnormalities of 27 metabolites were ameliorated by MHD. The analysis of metabolic pathways revealed that the therapeutic effects of MHD are likely due to the modulation of the metabolic disorders of tricarboxylic acid (TCA) cycle, retinol metabolism, tryptophan metabolism, arginine and proline metabolism, nicotinate and nicotinamide metabolism, phenylalanine metabolism, phenylalanine, tyrosine and tryptophan synthesis, as well as cysteine and methionine metabolism. This study demonstrated for the first time that MHD exerted an obvious protective effect against ALF mainly through the regulation of TCA cycle and amino acid metabolism, highlighting the importance of metabolomics to investigate the drug-targeted metabolic pathways.
研究领域:
药理学
·
肝病学
·
代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Functional Foods
·
2021
·
IF 4.451
Jie Gao,Manqian Zhang,Ruixue Niu,Xue Gu,Erwei Hao,Xiaotao Hou,Jiagang Deng,Gang Bai
Nutrients in diets, especially functional foods, are beneficial in metabolism-related diseases, such as diabetes. Cinnamaldehyde (CA), a natural flavorant, inhibits glycolysis while enhancing glucose storage. Kaempferol (KP), a flavonol in edible plants, inhibits amino acid metabolism and gluconeogenesis. Whether the combination of CA and KP exerts stronger effects in diabetes needs further investigation. In this work, nontargeted metabolomics results confirmed the simultaneous obstruction of glucose and amino acid metabolism by CA and KP. The falling status of the energy supply, demonstrated by the increased ratio of adenosine monophosphate (AMP) /adenosine triphosphate (ATP), was sensed by AMP-activated protein kinase (AMPK). The activation of AMPK in turn enhanced lipolysis and inhibited fatty acid synthesis both in vivo and in vitro. In conclusion, this study indicated that the CA and KP combination ameliorated glucose and lipid metabolism disorders by enhancing lipid metabolism via the activation of AMPK.
研究领域:
营养学
·
代谢学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 12
·1-8
·
IF 5.479
Hou Guorong,Yun Yanjing,Wang Minqiang,Wang Ying,Chen Hao,Zhang Longcheng,Wang Feng,Xia Qingyou,Liu Yang,Lu Zhisong,Bao Shu-Juan
A corn-like CeO 2 /C coaxial cable textured by a cerium oxide shell and a carbon core was designed to sense NO. The carbon core possesses high electrical conductivity, and the CeO 2 surface delivers excellent electrocatalytic activity. The sensor, typically operated at 0.8 V (vs. Ag/AgCl), exhibits a detection limit of 1.7 nM, which is 4-times lower than that of CeO 2 nanotubes based one (at S/ N = 3). It also displays wide linear response (up to 83 μM), a sensitivity of 0.81 μA μM −1 cm −2 , and fast response (2 s). These values are highly competitive to that of a CeO 2 tube (0.92 μA μM −1 cm −2 and 2 s). The sensor was used to quantify NO that is released by Aspergillus flavus.
Schematic representation of corn-like CeO 2 /C which can more sensitively and effectively detect NO released from A. flavus than when using CeO 2 nanotubes, benefitting from its unique coaxial cable structure.
研究领域:
材料科学
·
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHROMATOGRAPHIA
·
2019
·Vol. 82
·期 9
·1345-1354
·
IF 1.552
Pang Xiaomin,Bai Ligai,Wang Zijun,Yang Huijun,Liu Haiyan,Yan Hongyuan
A 5-cm-long polymer-based chromatographic monolith was fabricated and used as the stationary phase for high-performance liquid chromatography to quantitatively determine six aroma compounds in edible spices, namely, anethole from star anise, trans -cinnamaldehyde from cinnamon, hydroxy-alpha-sanshool from zanthoxylum, as well as 6-gingerol, 8-gingerol, and 10-gingerol from ginger. The prepared monolith was characterized using scanning electron microscopy, nitrogen adsorption–desorption method, and mercury intrusion porosimetry, respectively, indicating the relative uniform porous structure of the monolithic material. The quantitatively analytical method using the homemade monolithic column was validated, and the values of the relative standard deviation for precision were in the ranges of 0.27–8.90% for intra-day and 0.93–8.28% for inter-day measurements. The values of accuracy, expressed by spiked recovery, were all within the range of 92.02–118.59%. Furthermore, real species obtained from different habitats were detected using the present method. All of the results indicate that the present method is a simple, time-saving, inexpensive, and efficient method for quantitatively analyzing aroma components from edible spices, and exhibits good specificity and durability.
研究领域:
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2019
·
IF 3.579
Mostafa Gouda,Meihu Ma,Long Sheng,Xiaole Xiang
This study was focused on the differences between three concentrations of four safe bio-active volatile terpenes and natural compounds (trans-cinnamaldehyde, thymol, menthol, and vanillin) on the egg yolk volatile components. In which, Headspace Solid phase Micro Extraction (HS-SPME) followed by GC/MS were used for volatiles analysis, and electronic nose (E-Nose) with 18 sensors was used for the aroma pattern. In the results, a total of 111 different volatile compounds were identified by using GC/MS. Some ketones, amines, nitro and organic acid compounds such as hexanone were significantly reduced after using these compounds. On the other hand, first principal components (PC1) of trans-cinnamaldehyde (0.1%) was decreased significantly ( p < .05) to −4.8 ± 0.8, compared to the control with 4.3 ± 2.1. And, it increased benzaldehyde and nonanal peaks area significantly to 46.56 ± 14.46 and 9.22 ± 4.81 MPA. In menthol (0.1%), the content of d-limonene was decreased to 4.65 ± 0.49 MPA, compared to the control with 14.16 ± 4.95 MPA. On the other hand, by E-Nose, vanillin (0.1%) PC1 was decreased significantly to −4.48 ± 0.43. Moreover, thymol (0.1%) decreased hydrocarbon sensation to 0.448 ± 0.005. And, vanillin (0.1%) decreased hydrogen sulfide and ketones sensation to 0.169 ± 0.01 and 0.344 ± 0.01, respectively. In conclusion, this study is providing novel information to understand the effects of bio-active molecules on the biological fatty media volatility. Also, it explains how terpenes can protect egg yolk media from the unacceptable odor formation which results from the degradation of its lipids and proteins.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Parasites & Vectors
·
2021
·Vol. 14
·期 1
·1-11
·
IF 3.876
Nwanade Chuks F.,Wang Min,Wang Tianhong,Zhang Xiaoyu,Wang Can,Yu Zhijun,Liu Jingze
Background The tick Haemaphysalis longicornis (Neumann) is a well-known vector of numerous pathogens of veterinary and medical importance. Various control strategies, including the use of synthetic pesticides, have been developed to control this tick species. However, demand for effective and safe alternative pesticides is increasing due to the adverse effects associated with the intensive and injudicious use of synthetic pesticides, which include undesirable effects on non-target species and environmental pollution. Hence, the acaricidal activity of the extract and the essential oil of Cinnamomum cassia (Chinese cinnamon) and their major components, and the underlying mechanisms of this activity, were evaluated against unfed larvae and nymphs of H . longicornis . Methods The components of the extract and essential oil of C. cassia were determined by gas chromatography-mass spectrometry, and their larvicidal and nymphicidal activity were evaluated using the larval and nymphal packet test. The underlying detoxification mechanism was elucidated by targeting in vivo esterase and monooxygenase activity, and the toxicological effect was assessed on non-target Tenebrio molitor and Harmonia axyridis by topical application in open Petri dishes. Results ( E )-cinnamaldehyde was the predominant component of the extract (50.79%) and essential oil (89.95%). The 50% lethal concentration (LC 50 ) for larvae and nymphs treated with the extract was 11.56 and 49.18 mg/mL, respectively. The essential oil, ( E )-cinnamaldehyde and fenvalerate exhibited acaricidal activity, with LC 50 values of 3.81, 3.15, and 0.14 mg/mL, respectively, against the larvae, and 21.31, 16.93, and 1.89 mg/mL, respectively, against the nymphs. ( E )-cinnamaldehyde significantly increased esterase and monooxygenase activity in both larvae and nymphs. Unlike fenvalerate, C . cassia essential oil and ( E )-cinnamaldehyde did not cause mortality of T . molitor or H . axyridis adults. Conclusions This study demonstrates that C . cassia essential oil and ( E )-cinnamaldehyde have the potential to be developed into botanical-based larvicidal and nymphicidal agents for tick control. Graphical abstract
研究领域:
植物学
·
昆虫学
·
农药科学
·
兽医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2018
·
IF 5.089
Mostafa Gouda,Linlin Zu,Sumin Ma,Long Sheng,Meihu Ma
The objective of this study was to evaluate the effects of the natural safety high antimicrobial compounds on egg yolk protein structure and function. Three different concentrations have been evaluated from: trans-cinnamaldehyde, thymol, menthol or vanillin on egg yolk by measuring the antimicrobial activity, Fourier transform infrared (FT-IR), secondary protein structure, surface hydrophobicity, zeta-potential, foaming and color properties. Results showed that by mixing yolk with 0.1% of thymol and trans-cinnamaldehyde, the antimicrobial activity increased significantly ( P < 0.05) to 92.18 ± 3.26; 98.95 ± 3.16 Inh% against Salmonella typhimurium and 103.30 ± 4.58; 101.34 ± 1.82 Inh% against, E. coli . Also, from the results of FT-IR and Circular dichroism , trans-cinnamaldehyde, thymol and menthol (0.1%) changed yolk α-helix protein significantly (34.00 ± 0.85; 34.05 ± 0.21 and 34.15 ± 0.35) % compared to the control (32.70 ± 0.14) %. Meanwhile, the surface hydrophobicity and foaming capacity increased and zeta-potential decreased significantly as a result of terpene-protein cross-link formation by using these bio-active compounds. However, the foaming stability of trans-cinnamaldehyde (0.1%) decreased significantly after 30 and 60 min. Menthol (0.05, 0.1%) showed the most effects on color properties in which the lightness increased to 83.24 ± 0.27 and 83.67 ± 0.14 compared to the control (80.15 ± 0.44). Therefore, this study is opening new thinking idea about using the bio-active molecules in food hydrocolloidal field for enhancing their health ability.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）