LWT-FOOD SCIENCE AND TECHNOLOGY
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2025
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IF 6.6
Xiaoqin Wu,Zhiyi Shan,Lufan Wang,Wenwei Shi,Yanyan Li,Hongfang Cai,Liang Zhou,Chen Huan
Anthocyanins are bioactive substances that are not only responsible for the pigmentation of various fruits, but also hold potential benefits to human health. In this study, postharvest peaches were treated with 10 ppm 1-MCP, and changes of anthocyanin biosynthesis were then revealed at transcriptional level to gain insight into the underlying molecular mechanisms. The functions of key transcription factors (TFs) were systematically analyzed. Results demonstrated that 1-MCP effectively up-regulated expression of structural genes and stimulated anthocyanin accumulation. A novel transcription factor PpbHLH135 can respond positively to 1-MCP treatment and has been noted for its remarkable gene expression and protein abundance. The transcription factor bHLH3 was also investigated in this study due to its widespread interest and attention, serving to compare the regulatory function of bHLH135 in anthocyanin synthesis, although it didn’t show a significant response to 1-MCP treatment in this study. PpbHLH3 TF showed a stronger regulatory effect on PpF3H , while PpbHLH135 was more dominant over the regulation of PpUFGT . Future genetic and molecular experimentation will elucidate whether PpbHLH135 TF, in combination with other TFs, can play a stronger regulating role in fruit pigmentation, or in other metabolic pathways.
研究领域:
植物学
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分子生物学
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产品关联
DOI
高影响因子
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
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2025
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IF 3.3
Xiaorui Wang,Mingming Huang,Wenhui Li,Yingying Shi,Yinuo Tang,Han Zhang,Bin Wu,Zheng Zhang,Zunyang Song,Jingying Shi
Peach fruit is highly susceptible to Monilinia fructicola ( M. fructicola ), a pathogen that causes significant postharvest losses. Enhancing the efficacy and reducing the dosage of chemical fungicides are critical for controlling brown rot caused by M. fructicola . Natural volatile organic compounds (VOCs) have been proposed as a selective method for managing postharvest pathogen infection. Among these, 2-decanone, a natural volatile produced by fungi and insects, has demonstrated potential in inhibiting pathogen growth. In this study, 60 μL/L 2-decanone effectively inhibited the growth of M. fructicola mycelia. It also reduced spore germination and appressorium formation by downregulating the expression of MfBmp1 and MfPls1 , respectively. In addition, 2-decanone induced spore apoptosis by increasing reactive oxygen species (ROS) accumulation and exacerbating mitochondrial damage. When applied to peach fruit, 2-decanone fumigation significantly reduced disease incidence and lesion diameter compared to the control group, without adversely affecting fruit quality during storage. Furthermore, we observed that 2-decanone exhibited synergistic effects when combined with boscalid, effectively inhibiting the growth of M. fructicola . Applying boscalid 15 days before harvest and fumigating with 2-decanone postharvest provided similar brown rot suppression as boscalid alone but used only one-tenth the amount of fungicide. Overall, 2-decanone fumigation presents an effective strategy for controlling postharvest brown rot disease in peaches, and its combined use with boscalid introduces a promising approach for enhancing postharvest peach fruit preservation.
研究领域:
植物病理学
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采后生物学
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农药科学
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分子微生物学
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食品保鲜
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
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2020
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IF 6.306
Yi-Ge Zhang,Huan Kan,Sheng-Xiong Chen,Kiran Thakur,Shaoyun Wang,Jian-Guo Zhang,Ya-Fang Shang,Zhao-Jun Wei
The phenols in Diaphragma juglandis fructus (DJF), walnut pellicle (WP), and flowers of Juglans regia (FJR) from walnut were extracted using three methods (methanolic condensation reflux extraction, ultrasonic wave extraction, and enzyme assisted-extraction), and phenolics and antioxidant capacities of different extractions were compared. Overall, 50 phenolics were identified by HPLC-MS/MS with 41 compounds in DJF, 32 in WP, and 29 in FJR. It was observed that tannins in WP was higher than those in DJF and FJR. As for PCA, more than 70% of the variance was explained with the obvious comparison between the phenolic constituents. The phenolics in walnut contributed to remarkable antioxidant effect, with the highest effect observed in WP. This study presents the analysis and comparison of the phenols can be further extended for the development of functional walnut instant foods.
研究领域:
植物化学
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食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2021
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IF 4.556
Qiang Liu,Chen Ma,Kangli Wei,Kang Tu,Leiqing Pan
This work describes the optical approach for the quantitative description of sugar profiles in peaches during storage by using single integrating sphere (SIS). The spectral profiles for the optical properties (light absorption coefficient μ a and reduced scattering coefficient μ’ s ) were measured. Correlation analysis between the optical properties and the sugar profiles showed that higher correlated values, with r > 0.640, were found. Furthermore, quantitative models were constructed by Partial Least Squares Regression (PLSR) based on μ a and μ’ s datasets to predict the sugar composition in peach flesh. The predicted results for the sucrose content based on μ a datasets by PLSR were better than those based on the μ’ s . For fructose and glucose contents, the predicted models were not very satisfactory due to low concentration in peach flesh, with R p 2 of 0.721, RMSEP of 0.817 g kg −1 for fructose and R p 2 of 0.753 and RMSEP of 0.604 g kg −1 for glucose, respectively. The models established by SIS present a simple and efficient strategy for quantitative analysis and may be used as alternative methods for evaluating the quality attributes of different types of fruit.
研究领域:
食品科学
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光学检测
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DOI
高影响因子
FOOD CHEMISTRY
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2018
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IF 4.946
Li Wang,Timin Shan,Bing Xie,Chen Ling,Shuang Shao,Peng Jin,Yonghua Zheng
Glycine betaine (GB) treatment is useful to reduce chilling injury (CI) of several kinds of fruits including peach. However, the regulatory mechanism remains unknown. In this study, peach fruit was treated with 10 mmol L −1 GB solution for 10 min. The effects of GB treatment on CI, phenolic and soluble sugar metabolism were investigated in this study. Moreover, phenylpropanoid and soluble sugar content, and enzyme activities associated with phenolic and sugar metabolisms were also measured. The results showed that GB reduced CI and maintained high levels of total phenolic and flavonoid content. The activities of phenylpropanoid metabolism-related enzymes were significantly enhanced by GB. Higher content of sucrose and lower contents of fructose and glucose were observed in GB-treated fruits. Therefore, our results showed that GB could enhance chilling tolerance of peach through regulating phenolic and sugar metabolisms, and maintaining high levels of individual phenolic and sucrose content.
研究领域:
植物生理学
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食品科学
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农业化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
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2025
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IF 6.8
Zhimin Wang,Mingming Huang,Mei Dai,Dandan Tian,Yingying Shi,Bin Wu,Zheng Zhang,Zunyang Song,Jingying Shi
Peach fruit is highly susceptible to decay caused by Botrytis cinerea . Volatile compounds have attracted considerable attention due to their safety and practical application advantages. S-methyl butanethioate, a volatile organic compound, is biosynthesized by microorganisms and plants. In this study, fumigation with synthetic s-methyl butanethioate at 50, 100, 200 and 300 μL/L effectively inhibited mycelial growth and conidial germination of B. cinerea and disease incidence of gray mold on peach fruit in a concentration-dependent manner. Furthermore, s-methyl butanethioate induced conidial apoptosis by causing the accumulation of reactive oxygen species (ROS) and exacerbating mitochondrial damage. To address the limitations in solubility, stability, and volatility of s-methyl butanethioate, a slow-release fresh-keeping card was prepared by embedding s-methyl butanethioate into β-cyclodextrin. Scanning electron microscopy (SEM) and Fourier transform-infrared spectroscopy (FT-IR) confirmed the successful embedding of s-methyl butanethioate into the cavity of β-cyclodextrin, forming the encapsulated structure of the inclusion complex. Notably, the release of s-methyl butanethioate from the fresh-keeping card was delayed for 15 d and the antifungal effect was stronger than direct fumigation with s-methyl butanethioate. Moreover, the fresh-keeping card application prevented surface damage to peach fruit caused by direct fumigation. Therefore, the fresh-keeping card loaded with encapsulated s-methyl butanethioate not only enhanced stability but also extended antifungal efficacy, outperforming the traditional un-encapsulated form. Therefore, this study demonstrates that fresh-keeping card loaded with natural components might serve as an alternative to synthetic chemical fungicides for controlling postharvest diseases of fruit and vegetables.
研究领域:
植物病理学
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采后技术
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食品保鲜
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微生物学
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真菌生物学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
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2023
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IF 8.8
Jinlong Wu,Changwen Chen,Yong Li,Ke Cao,Xinwei Wang,Weichao Fang,Gengrui Zhu,Lirong Wang
The characterization of peach metabolites and carotenoids during canning and storage remains unclear. The present study identified 658 metabolites and 40 carotenoids in peach fruits throughout the canning and storage using ESI-MS/MS and APCI-MS/MS based metabolome approach. A total of 282 differentially accumulated metabolites were found, mainly including 78 phenolic acids, 74 lipids, 61 flavonoids. Five esterified carotenoids (rubixanthin palmitate, β-cryptoxanthin oleate, β-cryptoxanthin laurate, β-cryptoxanthin palmitate, and β-cryptoxanthin myristate) were the main peach carotenoids, with a proportion of approximately 90%, while free carotenoids accounted for 4.22–5.95% during the entire processing period. Moreover, the total carotenoid loss rates for canning and storage were 56.67% and 46.55%, respectively. Compared to the loss of free carotenoids, esterified carotenoids were more stable during storage, while canning led to a greater loss of esterified carotenoids. The results provided new insights into the maintenance of health-related phytochemicals from canning processes.
研究领域:
食品科学
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代谢组学
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农学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2024
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IF 4
Kaiyang Zhu,Ji Ma,Shuqi Hao,Ting Zhang,Hongjie Lei,Wenge Zhao,Huaide Xu,Mei Li
Oil body (OB) is composed of phospholipids, proteins and neutral lipids. However, the changes of these components in walnut OB (WOB) during walnut growth have not been studied. In this paper, CLSM, TEM and LC-MS/MS were used to observe and quantify the microstructure, accumulation level and biochemical composition of WOB during the growth of walnut (Wen 185 cultivar). The results showed that WOB accumulated near the cell wall on day 60 and then toward the center of cell until it occupied the entire cell on day 88. Afterwards, the WOB began to grow and fuse. Analysis of the chemical composition of WOB indicated that phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, and phosphatidylserine and oleosins, caleosins, and steroleosins made up the WOB membrane. The core of the WOB consisted of 22 free fatty acids and monoacylglycerol, diglyceride, triglyceride. Phosphatidylcholine, triglyceride and oleosin contents continued to increase. The contents of phosphatidylglycerol, unsaturated fatty acids, caleosins and steroleosins continued to decline. The content of phosphatidylethanolamine, phosphatidylinositol and phosphatidylserine remained basically unchanged. These findings provided a basis for understanding the dynamic pattern of WOB bioaccumulation in walnut kernel at different developmental stages.
研究领域:
植物生理学
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脂类生物化学
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食品科学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2012
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IF 3.898
Lili Zhao,Shuhua Zhu,Jie Zhou
A novel amperometric nitric oxide (NO) sensor was prepared by modifying an imprinted functional polymer P-l[Co II (salen)] (salen: bis(2-hydroxy-benzaldehyde)ethylenediimine) on the surface of a glassy carbon electrode (GCE) in the presence of multi-wall carbon nanotubes (MWCNTs) and chitosan (CS). Here P-l[Co II (salen)] functionalized as an artificial recognition receptor for NO instead of a traditional molecularly imprinted polymer in which the template molecule was usually adopted to generate specific binding sites for itself. The analytical performances of the NO sensor were evaluated by differential pulse voltammetry (DPV) and amperometric measurements ( i – t ). The experimental results indicate that the developed sensor exhibits an outstanding anti-interference ability and has linear relationship with NO concentration in the range of 7.2 × 10 −9 –9.0 × 10 −5 M with the detection limit of 2.99 × 10 −9 M (S/N = 3). Finally, the sensor was successfully applied to the detection of NO release from peach fruits.
研究领域:
电化学
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分析化学
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材料科学
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生物传感器
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）