JOURNAL OF FOOD SCIENCE
·
2024
·Vol. 89
·期 3
·1485-1497
·
IF 3.2
Xiaoying Li,Chunsheng Liu,Junkai Wu,Xiao Xiao,Libin Zhang,Caixia Chen,Annette S. Wilson,Fuhang Song
To recognize the key ester-related volatile compounds, 5 types of peaches including 54 late-ripening peach materials were examined by headspace solid-phase microextraction coupled with gas chromatography–mass spectrometry and E-nose. Here, a large number of esters were identified to be released by ripe peach fruits and were mainly characterized by fruity, green, and fatty notes. The variety and content of esters had greatly changed within or between cultivars, indicating that the fruit volatiles were highly differentiated depending on the specific genotypes and cultivation conditions. The ester types showed that fatty acid-derived C6 alcohols and methyl-/ethyl- short-chain alcohol were the main ester precursors, which were more likely to be utilized and well selected by alcohol acyltransferases, whereas the preference of acyl donors was not observed. The common peach type, which exhibited a unique volatile profile, displayed broader diversity and more abundant characteristics in ester-related volatiles than the other four types. A total of 19 key esters were identified as the main components and the content of most esters showed no significant difference among different peach types. Some key esters had even been enriched in nectarines. Moreover, the multiple discriminant analysis revealed a possible relationship between peach types and the domestication of the peach evolution. This study investigated ester-related volatiles released by different types of peach fruits and can be further used to evaluate the peach qualities, providing an important reference for peach breeding and processing.
研究领域:
植物学
·
食品科学
·
园艺学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Jia-Hui Ma,Li-Qiu Ren,Tian-Xuan Tang,Yu-Yao Chen,Chen-Xi Zhang,Yu-Feng Ke,Yan Zhang,Mitchell N. Muskat,Xiang-Rong Cheng
This study investigated the effects of polyphenols from walnut pellicle on the structure and allergenicity of walnut globulin (WG). The allergenicity-guided assay revealed walnut globulin (WG) from walnut defatted powder without pellicle was the most allergenic protein fraction of walnut. Complexes of phenolic extracts from walnut pellicle (PEWP) and WG were prepared in different ratio and pH conditions to further investigate the effect of PEWP on the protein structure and allergenicity of globulin. The covalent interaction between PEWP and WG in pH 7 was lower than pH 11, according to the free amino groups' content and SDS-PAGE analysis. Comprehensive methods characterized the complexes, including UV–Visible, fluorescence, Fourier transform infrared, and circular dichroism spectroscopy, and indicated that combination of polyphenols with protein altered the secondary and tertiary structure of the protein, leading to the unfolding of the protein structure. Meanwhile, the combination with PEWP reduced the IgG-binding capacity of WG in a dose-dependent manner, reaching a minimum of 26.90 ± 4.31% (at pH 7), which was significantly (P < 0.05) lower compared to that at pH 11 (41.79 ± 1.25%). Correlation analysis revealed a significant correlation between IgG-binding capacity and protein structure, as well as the addition concentration of PEWP (r = −0.85, P < 0.01), and pH conditions may indirectly influence the structure and allergenicity of the protein by affecting the interaction between WG and PEWP. The interaction between WG and PEWP was characterized through fluorescence-quenching mechanism analysis, indicating that polyphenols from PEWP mainly interact with WG through hydrogen bond and hydrophobic interaction.
研究领域:
食品科学
·
免疫学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING RESEARCH & DESIGN
·
2022
·
IF 4.119
Mengfei Tian,Chunyu Liang,Naveed Ahmad,Chunying Li,Weihao Wang,Yu Zhang,Zidan Luo,Lequn Qiu,Chunjian Zhao
A reverse micellar microemulsion combined with magnetic solid phase extraction (RMM-MSPE) technology was developed for the extraction and purification of juglone from Juglans mandshurica . Reverse micellar microemulsion (RMM) (Tween 80: n-propanol: n-hexane: 0.05 mol/L KCl aqueous solution = 27:13.5:4.5:55; w/w/w/w) was used to extract juglone based on microwave. The optimum conditions were a pH of 5.6, a liquid-solid ratio of 20:1 (mL/g), temperature of 40 °C, time of 63 s, and power of 400 W. Graphene oxide/nano diamonds/ Fe 3 O 4 (GO/ND/Fe 3 O 4 ) was prepared as adsorbent and applied to purify juglone by magnetic solid-phase extraction (MSPE). The optimum adsorption conditions were time of 14 h, solid to liquid ratio of 30 mg/mL, pH of 5.5 and shaker speed of 200 r/min. Anhydrous methanol provided the best desorption of juglone and the optimum conditions were a desorption time of 16 h, pH of 8, and the solid-liquid ratio of 40 mg/mL. The purity of juglone was 69.08% and the recovery yield was 78.67%. RMM and GO/ND/Fe 3 O 4 could be reused three times and eight times after the first use, respectively. The proposed RMM-MSPE is a green and efficient alternative method for extraction and purification of juglone.
研究领域:
植物化学
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环境科学
·
药用植物学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2019
·Vol. 42
·期 23
·3544-3552
·
IF 2.516
Xudong Xiao,Zhenqun Li,Ya Liu,Li Jia
An S-mandelic acid imprinted chitosan resin was synthesized by cross-linking chitosan with glutaraldehyde in 2% acetic acid solution. S-Mandelic acid imprinted chitosan resin was used to enantioselectively separate racemic mandelic acid in aqueous medium. When keeping the pH of sample solution (100 mM Tris-H 3 PO 4 ) at 3.5 and adsorption time at 40 min, the enantiomer excess of mandelic acid in supernatant was 78.8%. The adsorption capacities of S-mandelic acid imprinted chitosan resin for S- and R-mandelic acid were determined to be 29.5 and 2.03 mg/g, respectively. While the adsorption capacities of non-imprinted cross-linked chitosan for S- and R-mandelic acid were 2.10 and 2.08 mg/g, respectively. The result suggests that the imprinted caves in S-mandelic acid imprinted chitosan resin are highly matched with S-mandelic acid molecule in space structure and spatial arrangement of action sites. Interestingly, the enantiomer excess value of mandelic acid in supernatant after adsorption of racemic mandelic acid by R-mandelic acid imprinted cross-linked chitosan was 25.4%. The higher enantiomer excess value by S-mandelic acid imprinted chitosan resin suggests that the chiral carbons in chitosan and the imprinted caves in S-mandelic acid imprinted chitosan resin combine to play roles for the enantioselectivity of S-mandelic acid imprinted chitosan resin toward S-mandelic acid. Furthermore, the excellent enantioselectivity of S-mandelic acid imprinted chitosan resin toward S-mandelic acid demonstrates that using chiral chitosan as functional monomer to prepare molecularly imprinted polymers has great potential in enantioseparation of chiral pharmaceuticals.
研究领域:
分析化学
·
生物材料学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2025
·Vol. 90
·期 1
·e17604
·
IF 3.4
Yujiao Zhang,Li Zhang,Like Lin,Yiran Zhang,Cong Li,Bang Chen,Yehua Shen
The purpose of this article is to investigate the effects of walnut ( Juglans regia L.) kernel pellicle on the composition and properties of enzymatic hydrolysis products of walnut meal using peptidomics and bioinformatics. In this study, a total of 3423 peptide sequences were identified in peeled walnut protein hydrolysates (PWPH) and unpeeled walnut protein hydrolysates (UWPH). Due to the presence of the walnut kernel pellicle, the enzyme cleavage sites of alkaline proteases on walnut precursor proteins were altered, resulting in differences in the number and length of the peptides obtained. Principal component analysis indicates significant differences between PWPH and UWPH. Combined with bioinformatics analysis, it was shown that walnut kernel peeling improved the release of peptides, formed more bioactive peptides, reduced allergenicity, and improved water solubility. Seven peptides with acetylcholinesterase (AChE) inhibitory activity were identified, and the peptide Val-Gly-Ala-Pro-Phe-Asp-Gly-Ala (VGAPFDGA) has the strongest inhibitory activity with an IC 50 of 0.38 ± 0.01 mg/mL. These results confirmed that walnut kernel peeling could greatly change the composition of the walnut protein hydrolysates, and seven novel peptides were reported that showed significant AChE inhibitory activity.
研究领域:
食品科学
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生物化学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
·
2018
·
IF 1.845
Binbin Shi,Wene Zhang,Xue Li,Xuejun Pan
The phenolics content and antioxidant activity of walnut (Juglans sigillata Dode) green husks were analysed during different stages of development. Contents of total phenolics and total flavonoids decreased from April 25th to June 7th, then increased during June and July, and decreased again at the beginning of August. Thirteen types of phenolic compounds were identified using HPLC of which juglone had the highest content. Because of seasonal variations, the trends of different phenolic compounds were different. Contents of both total phenolics and total flavonoids were strongly correlated with antioxidant activity; however, compared with totals, less significant correlations were observed between individual phenolic compounds and antioxidant activities.
研究领域:
植物科学
·
食品科学
·
植物化学
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2024
·Vol. 13
·期 9
·1313
·
IF 4.7
Ping Li,Yujia Zhang,Changwei Cao,Yaxi Luo,Huan Kan,Yun Liu
Walnuts play a positive role in human health due to their large amounts of unsaturated fatty acids, whereas lipid oxidation can easily occur during storage. Herein, three natural antioxidants (epicatechin, sesamol, and myricetin) were added to the composite film cross-linked with chitosan and soy protein peptide, and the antioxidant film appropriate for the preservation of walnut kernels fromJuglans sigillatawas screened to improve the storage quality of walnuts. The results showed that three antioxidant films could all enhance the storage performance of walnut kernels, with sesamol being the best. The characterization of antioxidant film cross-linked with chitosan and soy protein peptide containing sesamol (C/S-ses film) revealed that the composite film improved the slow release and stability of sesamol; in addition, the presence of sesamol could effectively reduce the light transmittance and water vapor permeability of the composite film, together with significantly enhancing the antioxidant and antimicrobial activities, resulting in an effective prolongation of the storage period of walnut kernels. These findings indicated that C/S-ses possess excellent potential for retarding the oxidative rancidity of unsaturated fatty acids and will provide an effective strategy for the preservation of walnut kernels.
研究领域:
食品科学
·
材料科学
·
农业工程
✓ 已验证
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 28
·期 22
·28732-28749
·
IF 4.223
Dovi Evans,Kani Alexander Nti,Aryee Aaron Albert,Jie Ma,Li Jianjun,Li Zhaohui,Qu Lingbo,Han Runping
In this research, the eco-friendly cationic surfactant modified walnut shell (WNS-CTAB) was synthesised to enhance the uptake for bisphenol A (BPA) and Congo red (CR) from aqueous solution. The characterisation of WNS-CTAB was performed using Fourier-transform infrared (FTIR), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), etc. to know its physiochemical properties. The adsorption equilibrium results were best described by the Langmuir isotherm model, which confirmed the monolayer adsorption of the pollutant molecules onto the adsorbent’s surface. The maximum monolayer adsorption quantity of WNS-CTAB was established to be 38.5 mg g –1 for BPA and 104.4 mg g –1 for CR at 303 K, respectively. Pseudo-second-order kinetic models described the adsorption kinetics of both BPA and CR. Furthermore, the intra-particle diffusion was applied to analyse the kinetic results and was established that the rate was not solely controlled by diffusion. The mechanisms associated with BPA and CR adsorption onto the WNS-CTAB may include van der Waals interaction, hydrophobic interaction, and electrostatic force. WNS-CTAB demonstrated a good reusability potential with desorption through three successive adsorption-desorption cycles performed in both experiments. Moreover, in the binary system, the adsorption capacity of BPA witnessed a 66% decrease while CR saw marginal reduction of 8.0 %. This suggests that WNS-CTAB had a higher affinity for binding to CR with higher selectivity as compared with BPA. Therefore, WNS-CTAB has exhibited huge potential to serve as a functional material for practical use in the treatment of wastewater. Graphical abstract
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Xin Guo,Fengying Gu,Tingting Yang,Zhixiao Shao,Qiaozhen Zhang,Jinjin Zhu,Feng Wang
Ellagitannins, the main components of walnut kernel polyphenols, are easily degraded by heating to produce ellagic acid, precursor in the production of urolithins that show multiple physiological functions. To analyze the conversion of ellagitannins to free ellagic acid in walnut kernels during baking, a quantitative method was developed to investigate the ellagic acid content (EAC) in free phenolic acid (FPA), acid-hydrolyzable phenolic acid (AHPA), and bound phenolic acid (BPA) fractions. The results showed that the EAC in FPA reached its maximum (5.17 ± 0.30 mg/g DW) after baking at 165 °C for 30 min, which increased by 99.52% compared with the control. Meanwhile, the content of ellagitannins (ETC) in AHPA and BPA dropped by 89.14% and 26.08%, respectively. It suggested that baking promoted the conversion of ellagitannins in AHPA and BPA to ellagic acid in FPA. Eight ellagitannins were regarded as the main precursors of ellagic acid in walnut kernels.
研究领域:
食品科学
·
植物化学
✓ 已验证
DOI
PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 3.475
Hong-Yan Wang,Yu-Li Xie,Xin Shi,Hong-Ling Shi,Jian-He Xu,Cun-Duo Tang,Lun-Guang Yao,Yun-Chao Kan
Halogenated mandelate and its derivatives are important scaffolds in chemical industry. However, the natural D -mandelate dehydrogenase (DMDH) showed relatively low catalytic activity toward D -o-chloromandelic acid, which largely limits the efficiency of dehydrogenation processes. To improve catalytic activity of Lactobacillus brevis D -mandelate dehydrogenase ( Lb DMDH) toward halogenated substrate, a D -mandelate dehydrogenase mutant Lb DMDH N253S with higher catalytic activity toward D -o-chloromandelic acid was successfully obtained by directed evolution, which was about 29 times of Lb DMDH. In addition, the temperature optimum of Lb DMDH N253S is 50 °C, and closer to the optimal growth temperature for Escherichia coli , indicating that it may be more advantageous in whole cell catalysis using living cells as catalysts. Meanwhile, the K cat value toward D -o-chloromandelic acid of Lb DMDH N253S is 2.08 s −1 , which is significantly higher than the one (0.09 s −1 ) of Lb DMDH, indicating that Lb DMDH N253S displayed higher catalytic efficiency toward D -o-chloromandelic acid. Then, we preliminarily explained the molecular basis for its catalytic performance by in silico design methods. All of these results established a solid foundation for molecular modification of DMDHs and the other industrial enzymes.
研究领域:
生物化学
·
酶工程
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2017
·
IF 4.811
Bo Huang,Meice Lu,Dali Wang,Yiheng Song,Li Zhou
In this paper, we report a facile approach to prepare magnetic gel based on the simultaneous formation of Fe 3 O 4 nanoparticles and crosslinking of natural peach gum polysaccharide . The obtained magnetic gel with porous structure, high specific surface area and numerous oxygen-containing functional groups exhibited fast and efficient adsorption capability towards Pb 2+ and Cd 2+ ions. The adsorption isotherms and kinetics presented good correlation with Langmuir isotherm and pseudo-second-order model, respectively. The maximum adsorption capacity of the magnetic gel for Pb 2+ and Cd 2+ ions can reach 277.0 and 141.4 mg g −1 , respectively, which is much higher than many other reported magnetic adsorbents. In addition, the magnetic gel can serve as a magnetic support for in situ growth of Pt nanocatalyst with high catalytic activity, as demonstrated by the reduction of 4-nitrophenol. Moreover, the magnetic gel exhibited excellent magnetic separation capability and superior reusability both for the adsorption and catalysis applications.
研究领域:
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2014
·
IF 4.258
Chun Wang,Ruiyang Ma,Qiuhua Wu,Meng Sun,Zhi Wang
In this paper, porous carbon with a highly ordered structure was synthesized using zeolite ZSM-5 as a template and sucrose as a carbon source. Through the in situ reduction of Fe 3+ , magnetic property was successfully introduced into the ordered porous carbon, resulting in a magnetic porous carbon (MPC). MPC was used as an adsorbent for the extraction of some chlorophenols (2-chlorophenol, 3-chlorophenol, 2,3-dichlorophenol and 3,4-dichlorophenol) from water and peach juice samples followed by high performance liquid chromatography-ultraviolet detection. Good linearity was observed in the range 1.0–100.0 ng mL −1 and 2.0–100.0 ng mL −1 for water and peach juice sample, respectively. The limits of detection ( S / N = 3) were between 0.10 and 0.30 ng mL −1 . The relative standard deviations were less than 5.3% and the recoveries of the method for the compounds were in the range from 87.8% to 102.3%. The results demonstrated that the MPC had a high adsorptive capability toward the four chlorophenols from water and peach juice samples.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）