INDUSTRIAL CROPS AND PRODUCTS
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2020
·
IF 4.244
Meng Pan,Qicheng Lei,Hong Zhang
Screening of active ingredients in plants is critical for the development of medicinal plants. As a traditional medicinal material, Eucalyptus globulus Labill leaves are regarded as a potential raw material that can be developed as a pesticide because of its original activity. This study proposes a molecular docking assisted UPLC-MS/MS, GC–MS/MS method for the identification and prediction of insecticidal ingredients in the extracts of Eucalyptus globulus leaves. The extracts of Eucalyptus globulus leaves were analyzed by UPLC-MS/MS, GC–MS/MS, 34 aqueous ingredients and 35 volatile ingredients were identified. The identified ingredients were docked with repellent receptor (2KSL). The results showed that protocatechuic acid had strong 2KSL inhibitory activity, which was presumed to be the main reason for the inhibition of 2KSL. Finally, the antifeedant experiment of protocatechuic acid on Henosepilachna vigintioctopunctata (Fabricius) was carried out. The results indicate that the method can effectively screen the active ingredients from the medicinal plants.
研究领域:
植物学
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药理学
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昆虫学
✓ 已验证
产品关联
DOI
高影响因子
CIENCIA RURAL
·
2022
·
IF 0.901
Liu Zhenyi,Wang Jing,Yin Meijuan,Liu Mengsheng,Gao Weijuan
The Eucalyptus plant releases allelopathic chemicals into the environment mostly through the essential oils volatilized from the leaves. This study discussed the composition of the leaf oils of few seven-year-old varieties like Eucalyptus pellita (E. pel), Eucalyptus camaldulensis (E. cama), Eucalyptus grandis (E. gra), Eucalyptus dunni (E. dun), Eucalyptus saligna (E. sal), and E. grandis × E. urophylla (E. gra×E. uro) and three-year-old E. grandis × E. urophylla (E. gra × E. uro (three)). It determined the allelopathic mechanism and the types of chemical components playing the leading role. Essential oil was obtained by hydrodistillation and analyzed by the Gas Chromatography-Mass Spectrometry (GC-MS) method. In order to determine the effect of allelopathy, seed germination experiments were carried out at different concentrations (10-100 mL/L) of the E. Gra × E. uro leaf oil (EO) and the major components. The wheat seeds germinated by adding 1,8-cineole were used to determine the activity of α-amylase. Moreover, the mRNA expression of α-amylase in seeds was studied. The major chemical class in the essential oil was oxygenated monoterpene; 1,8-cineole (20.2-67.5%) displayed the highest content. Other substances that were high in content and ubiquitous included α-pinene (0.3-21.8%), α-terpineol (0.44-19.24%), and borneol (0.81-3.05%). The four chemical constituents and EO influenced the germination and growth of the three plants. Among them, 1,8-cineole exhibited the strongest inhibitory effect. The α-amylase activity of the 1,8-cineole-treated wheat seeds had decreased significantly. Molecular evidence suggested that 1,8-cineole decreased the α-amylase gene (AMY) expression.
研究领域:
植物学
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生态学
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分子生物学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
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2022
·
IF 6.633
Congcong Chen,Ying Xie,Lingyi Jia,Yong Zhang
Efficient capture of Th(IV) from aqueous solution is of importance to both solve the radioactive pollution for environmental safety and provide clean energy for sustainable development. However, it still faces huge challenge. In this study, eucalyptus leaf extract was applied as template agent to fabricate Zn/Al-bimetallic layered double oxides (ZnAlLDO), providing more active sites for Th(IV) removal. The removal process of Th(IV) onto ZnAlLDO could reach equilibrium within 5 min and the maximum removal capacity calculated from the Langmuir model was 1153.71 mg g −1 at pH = 4.0 and 298 K. Over 97 % Th(IV) could be removed by ZnAlLDO during the dynamic adsorption process and the removal efficiency still remained at a high level (85 %) after 5 cycles. The Th(IV) removal process on ZnAlLDO was ascribed to electrostatic attraction, complexation and ion exchange. It could be believed that the ZnAlLDO with large removal capacity (1155.5 mg g −1 ), fast equilibrium time (5 min) and easy desorption (91.76 %) with high recovery (85 %) might be a promising adsorbent for the separation of Th(IV).
研究领域:
环境科学
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材料科学
✓ 已验证
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2017
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IF 3.387
Chengyang Li,Zechao Zhuang,Xiaoying Jin,Zuliang Chen
In this paper, a green and facile synthesis of reduced graphene oxide (GO) by Eucalyptus leaf extract (EL-RGO) was investigated, which was characterized with ultraviolet-visible spectroscopy (UV), Raman spectroscopy, X-ray diffraction (XRD), scanning electron microscope (SEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and Thermal gravimetric analysis (TG). Eucalyptus leaf extract also play both reducing and capping stabilizing agents prepared EL-RGO as shown a good stability and electrochemical properties. This approach could provide an alternative method to prepare EL-RGO in large-scale production. Moreover, the good electrochemical property and biocompatibility can be used in various applications. In addition, the merit of this study is that both the oxidized products and the reducing agents are environmental friendly by green reduction.
研究领域:
材料科学
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环境科学
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生物学
✓ 已验证
产品关联
DOI
高影响因子
CHEMOSPHERE
·
2018
·
IF 4.427
Xiaoying Jin,Na Li,Xiulan Weng,Chengyang Li,Zuliang Chen
Reduction of graphene oxide (RGO) utilizing green methods such as plants has attracted much attention due to its efficiency, eco-friendly features and low cost. However, the key components in plant extracts and their bioreduction functions concerning GO are still not well understood. In this study, the GO was reduced by Eucalyptus leaf (EL) extract. The optimal conditions for bioreduction were at volume ratio of leaf extract (10 g L −1 ) and GO (0.5 g L −1 ) solution of 1:4 for 8 h at 80 °C. The RGO was characterized with Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDS), transmission electron microscopy (TEM), and Fourier Transform Infrared Spectroscopy (FTIR). Results confirmed the oxygen-containing groups in GO were efficiently removed, formation of capping layer on the surface of RGO, and good dispersion of RGO in aqueous solution. Furthermore, biomolecules were identified by Gas Chromatography-Mass Spectrometer (GC-MS), where eucalyptols, aldehydes, terpineols, alcohols, amides and ethers of Eucalyptus leaf extract may act as reducing and capping agents for the formation of RGO. Finally, the methyl blue (MB) adsorption on EL-RGO, activated carbon, graphite powder and commercial graphene were investigated separately. The order of the maximum adsorption capacity of different adsorbents emerged as: EL-RGO > commercial graphene > activated carbon > graphite powder.
研究领域:
材料科学
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环境科学
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植物学
✓ 已验证
DOI
高影响因子
ENVIRONMENTAL POLLUTION
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2019
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IF 5.714
Kaixi Wang,Yong Liu,Xiaoying Jin,Zuliang Chen
Biosynthesis of nanoparticles using plant extract is one kind of the effective approach in developing rapid, clean, nontoxic, and eco-friendly technology . In this work, iron nanoparticles/reduced graphene oxide composites (Fe NPs/rGO) were facilely fabricated through one-step method with eucalyptu s leaf extract. The synthesized Fe NPs/rGO was investigated by various characterization methods. SEM results disclosed that Fe NPs with regular spherical shape of 70 ± 10 nm was uniformly dispersed on rGO. A basal plane of rGO with high surface area, providing more load sites for Fe NPs, which was confirmed by TEM . XRD and FTIR results indicated that biomolecules from eucalyptus extract were capped on Fe NPs/rGO. The EDS mapping showed that Fe NPs dispersed evenly on rGO, and XPS further confirmed that Fe NPs/rGO composite was mainly comprised with Fe NPs and rGO. Furthermore, the biomolecules of eucalyptu s leaf extract were identified by GC-MS to confirm that alcohol phenols acted as reducing agent, while alcohol acids and ketones acted as capping agents. In addition, the removal efficiency of Methylene blue (MB) up to 93% with Fe NPs/rGO. This work provides a simple, green cost-effective and environmentally friendly method to fabricate metal/rGO composite.
研究领域:
材料科学
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环境科学
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生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2019
·Vol. 20
·期 16
·3875
·
IF 4.183
Meng Pan,Qicheng Lei,Ning Zang,Hong Zhang
The discovery of medicinal plants is crucial for drug development.Eucalyptus globulusleaves are used as a traditional medicine in many areas of world due to herbicidal and insecticidal activity. While natural products are difficult to be separated and activity assayed, a new approach is needed to predict the active ingredients therein. In this study, a new method for screening active compounds extracted fromE. globulusleaves was developed by GC-MS/MS and UPLC-MS/MS combined with molecular docking technology. Predicted compounds with high activity were proposed. Firstly, 35 volatile compounds and 34 aqueous extracted compounds were extracted fromE. globulusleaves, and identified by GC-MS/MS and UPLC-MS/MS. The herbicidal receptor (1BX9) was then docked with the identified compounds by docking software, evaluated by docking models and seven scoring functions. The results showed that gallic acid had a strong inhibitory activity of 1BX9, which was speculated to be the main reason for the inhibitory effect ofE. globulusleaves. Finally, allelopathic tests of gallic acid, citric acid, and isopulegol were carried out on grass seeds to verify its inhibitory activity against herbicide receptor 1BX9. The results show that the method can screen compounds with specific activity from a complex system of medicinal plants, which is very important for the screening of new active ingredients, confirmation of new medicinal ingredients, and the in-depth development of animal and plant medicines.Keywords:Eucalyptus globulus;GC-MS/MS;UPLC-MS/MS;molecular docking;allelopathic test
研究领域:
植物化学
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药物开发
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生物信息学
✓ 已验证
DOI
高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Heng Li,Kaisheng Gong,Xiaoying Jin,Gary Owens,Zuliang Chen
Since antimony (Sb) is a toxic metalloid cost-effective method for the simultaneous removal of the two major Sb species from mining wastewater has attracted much attention. In this study, phytosynthesized iron nanoparticles ( n Fe) prepared using a eucalyptus leaf extract were successfully used to simultaneously remove Sb(III) and Sb(V) via an adsorption and oxidation mechanism with removal efficiencies of 100 and 97.7% for Sb(III) and Sb(V), respectively. Advanced analysis using X-ray photoelectron spectroscopy (XPS), ion chromatography-atomic fluorescence spectroscopy (IC-AFS), and electrochemical analysis confirmed that Sb(III) was oxidized to Sb(V) by Fe(III) on the n Fe surface while Scanning Electron Microscopy (SEM) and Energy Dispersive Spectroscopy (EDS) indicated that both Sb(III) and Sb(V) were adsorbed onto n Fe. Adsorption of both Sb(III) and Sb(V) best fit the Langmuir adsorption model with R 2 of 0.999 and 0.989, respectively and both followed pseudo-second-order kinetics with R 2 of 0.999 and 0.981, respectively. Furthermore, the adsorption rate of Sb(III) was faster than that of Sb(V) due to inner-sphere complex formation, and the Fe–O bonds in the asymmetric tetrahedron structure of Sb(III) were easier to break due to a lower energy barrier (0.863 eV). Consequently, a simultaneous removal mechanism of Sb(III) and Sb(V) was proposed. Finally, n Fe was used practically to remove Sb in mining wastewater with a removal efficiency of 93.5%, demonstrating that n Fe have significant potential to remove Sb in contaminated mining wastewaters.
研究领域:
环境科学
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纳米技术
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水处理
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材料科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）