CIENCIA RURAL
·
2022
·
IF 0.901
Liu Zhenyi,Wang Jing,Yin Meijuan,Liu Mengsheng,Gao Weijuan
The Eucalyptus plant releases allelopathic chemicals into the environment mostly through the essential oils volatilized from the leaves. This study discussed the composition of the leaf oils of few seven-year-old varieties like Eucalyptus pellita (E. pel), Eucalyptus camaldulensis (E. cama), Eucalyptus grandis (E. gra), Eucalyptus dunni (E. dun), Eucalyptus saligna (E. sal), and E. grandis × E. urophylla (E. gra×E. uro) and three-year-old E. grandis × E. urophylla (E. gra × E. uro (three)). It determined the allelopathic mechanism and the types of chemical components playing the leading role. Essential oil was obtained by hydrodistillation and analyzed by the Gas Chromatography-Mass Spectrometry (GC-MS) method. In order to determine the effect of allelopathy, seed germination experiments were carried out at different concentrations (10-100 mL/L) of the E. Gra × E. uro leaf oil (EO) and the major components. The wheat seeds germinated by adding 1,8-cineole were used to determine the activity of α-amylase. Moreover, the mRNA expression of α-amylase in seeds was studied. The major chemical class in the essential oil was oxygenated monoterpene; 1,8-cineole (20.2-67.5%) displayed the highest content. Other substances that were high in content and ubiquitous included α-pinene (0.3-21.8%), α-terpineol (0.44-19.24%), and borneol (0.81-3.05%). The four chemical constituents and EO influenced the germination and growth of the three plants. Among them, 1,8-cineole exhibited the strongest inhibitory effect. The α-amylase activity of the 1,8-cineole-treated wheat seeds had decreased significantly. Molecular evidence suggested that 1,8-cineole decreased the α-amylase gene (AMY) expression.
研究领域:
植物学
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生态学
·
分子生物学
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产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2017
·
IF 5.651
Xiudong Zhang,Yuanyuan Bai,Xuefei Cao,Runcang Sun
Herein, an efficient biphasic pretreatment process was developed to improve the production of furfural (FF) and glucose from Eucalyptus . The influence of formic acid and NaCl on FF production from xylose in water and various biphasic systems was investigated. Results showed that the addition of formic acid and NaCl significantly promoted the FF yield, and the biphasic system of MIBK (methyl isobutyl ketone)/water exhibited the best performance for FF production. Then the Eucalyptus was pretreated in the MIBK/water system, and a maximum FF yield of 82.0% was achieved at 180 °C for 60 min. Surface of the pretreated Eucalyptus became relatively rough and loose, and its crystallinity index increased obviously due to the removal of hemicelluloses and lignin. The pretreated Eucalyptus samples showed much higher enzymatic hydrolysis rates (26.2–70.7%) than the raw Eucalyptus (14.5%).
研究领域:
生物化学
·
可再生资源工程
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产品关联
DOI
PubMed
高影响因子
AICHE JOURNAL
·
2024
·Vol. 70
·期 9
·e18495
·
IF 3.5
Jialin Tan,Hao Huang,Hengde Li,Xiaolan Cai,Guangping Zhou,Boxi Zeng,Jinrui He,Xiang Wang,Shaoqu Xie
The biobutanol-based biofuel fermentation is hindered by the challenge of inadequate fermentation broth concentration and the azeotropic systems of water. The application of eucalyptus oil in diesel fuel makes it a promising extractant for biobutanol extraction and dehydration, thus resulting in product recovery and the development of fuel blend technology. Eucalyptus oil-derived 1,8-cineole was applied to acetone-butanol-ethanol (ABE) and isobutanol systems to enhance the butanol recovery and dehydration. The recovery of n-butanol and isobutanol reached 92% and 95%, respectively, while the water removal in a 2% butanol system exceeded 99%. These results demonstrated that 1,8-cineole exhibited a high selectivity toward butanol, thus providing a robust theoretical foundation for the fermentation-based biofuel recovery and the elimination of azeotropic systems. The introduction of high-density diesel-fuel-like eucalyptus oil into low-energy-density biobutanol will increase the energy density of fermentation-based alcohols, yielding linear and cyclic biofuel blends.
研究领域:
生物化学
·
能源生物学
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产品关联
DOI
高影响因子
Frontiers of Chemical Science and Engineering
·
2024
·Vol. 18
·期 11
·1-14
·
IF 4.3
Mo Shuhua,Zheng Yao,Gong Jianyu,Lu Minsheng
The efficient fractionation and recovery of monosaccharides (xylose and glucose) from lignocellulosic biomass facilitates subsequent sugar-based derivative production. This study introduces a one-pot γ -valerolactone/ CuCl 2 biphasic pretreatment system (100-mmol·L -1 CuCl 2 , 180 °C, 60 min) capable of achieving removal rates of 92.25% and 90.64% for xylan and lignin, respectively, while retaining 83.88% of cellulose. Compared to other metal chlorides (NaCl, LiCl, FeCl 3 , and AlCl 3 ), the γ -valerolactone/CuCl 2 system recovered 121.2 mg·(g eucalyptus) -1 of xylose and 55.96 mg·(g eucalyptus) -1 of glucose during the pretreatment stage and 339.2 mg·(g eucalyptus) -1 of glucose during the enzymatic hydrolysis stage (90.78% of glucose yield), achieving a total monosaccharide recovery of 86.31%. In addition, the recovery of γ -valerolactone was 79.33%, exhibiting minimal changes relative to the pretreatment performance. The method proposed in this study allows a high total monosaccharides recovery and a circular economy-oriented pretreatment approach, offering a viable pathway for biorefinery.
研究领域:
生物工程
·
可再生资源技术
✓ 已验证
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2021
·
IF 9.642
Yadan Luo,Yan Li,Liming Cao,Jiatian Zhu,Baojuan Deng,Yajun Hou,Chen Liang,Caoxing Huang,Chengrong Qin,Shuangquan Yao
Glycolic acid has chemical properties similar to those of formic acid. Therefore, similar to formic acid pretreatment, glycolic acid pretreatment has the separation effect of hemicellulose. In this study, eucalyptus hemicellulose was effectively separated by glycolic acid pretreatment. The effects of glycolic acid concentration, temperature and time on the separation of cellulose, hemicellulose and lignin were investigated. The optimum conditions were acid concentration 5.40%, temperature 140 °C, time 3.0 h. The highest yield of xylose was 56.72%. The recovery rate of glycolic acid was 91%. Compared to formic acid, the yield of xylose increased to 10.33% while that of lignin decreased to 11.08%. It showed high selectivity for hemicellulose separation, yielding 65.48% hemicellulose with 72.08% purity. The depolymerization and repolymerization of lignin were inhibited. The integrity of the cellulose structure was preserved. It provides theoretical support for the fractional separation and high-value transformation of lignocellulosic biomass.
研究领域:
生物化学
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生物质能
·
林业工程
✓ 已验证
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2019
·
IF 4.191
Xiaoyun Li,Jiaxin Yang,Rui Xu,Lefu Lu,Fangong Kong,Min Liang,Lijie Jiang,Shuangxi Nie,Chuanling Si
The production of platform chemicals from lignocellulosic biomass is scientifically valuable but technologically challenging. With the aim to establish a green reaction system for the production of furfural from lignocellulosic biomass, in the current work, H-SAPO-34 was investigated for catalysis of the conversion of Eucalyptus sawdust to furfural in γ-valerolactone (GVL). H-SAPO-34 was prepared and characterized by XRD, SEM, FTIR, BET, TGA and ammonia adsorbed FTIR. H-SAPO-34 possesses Brønsted and Lewis acid sites and a large specific surface area. The average pore diameter of H-SAPO-34 was 0.86 nm, which is beneficial for the spread of xylose and furfural and the inhibition of humin production. In this work, a peak furfural yield of 99.28% from Eucalyptus was achieved at 210 °C in 120 min with 30 g/L H-SAPO-34. The Brønsted/Lewis acid bifunctionality and the solvent effect of γ-valerolactone possibly contributed to the high furfural yield from real biomass. The kinetics of the conversion of Eucalyptus sawdust to furfural was also studied, and the activation energy for furfural production was calculated. Additionally, the catalytic activity and stability of the regenerated catalyst were estimated.
研究领域:
化学工程
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可再生资源与环境科学
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产品关联
DOI
高影响因子
PLANT CELL AND ENVIRONMENT
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2024
·
IF 6
Kai Wang,Wenzi Ren,Liang Hong,Qingao Wang,Rajendra Ghimire,Matti Haapanen,Minna Kivimäenpää,Pengfei Wu,Xiangqing Ma,Fred O. Asiegbu
Norway spruce is an important coniferous species in boreal forests. Root and stem rot diseases caused by the necrotrophic pathogen Heterobasidion parviporum threaten the wood production of Norway spruce which necessitates the search for durable control and management strategies. Breeding for resistant traits is considered a viable long-term strategy. However, identification of potential resistant traits and markers remains a major challenge. In this study, short-term disease resistance screening was conducted using 218 Norway spruce clones from 17 families. Disease resistance was evaluated based on the size of necrosis lesion length following infection with the pathogen. A subset of needles/branches from clones with small (partial resistant) or large (susceptible) lesions were used for terpene analysis and transcriptomic profiling. The results revealed that the content of monoterpene linalool and 1,8-cineole and their respective encoded genes were significantly more abundant and highly expressed in the partial resistant group. Furthermore, linalool and 1,8-cineole were demonstrated to have inhibitory effect on the growth of the pathogen H. parviporum , with morphological distortion of the hyphae. RNAseq analysis revealed that transcript of pathogen genes involved in the regulation of carbohydrate metabolism and stress responses were significantly decreased in presence of the terpenes. The results suggest the relevance of monoterpenes together with jasmonic acid precursor and some genes involved in phenylpropanoid biosynthesis, as constitutive tolerance factors for Norway spruce tolerance against necrotrophic pathogen. The high level of necrosis related cell death gene expression might be factors critical for host susceptibility and disease development.
研究领域:
植物学
·
分子生物学
·
林学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2022
·
IF 6.449
Yachong Zhu,Yehong Chen,Chaojun Wu,Dongmei Yu,Qijun Ding,Ronggang Li
In this study, cellulose nanofibrils (CNFs) were directly prepared after autohydrolysis of eucalyptus raw materials. The effect of different autohydrolysis times on the hydrogen bond structure, micro-structure and properties of CNFs were analyzed by FTIR peak fitting, two-dimensional infrared, TGA, TEM and X-ray diffractometer. The results showed that the autohydrolysis treatments could obviously lead to the sequence of hydrogen bond changes in CNFs as follows: v (weak hydrogen bond) > v (free hydroxyl) > v (O(3)H.O(5)) > v (O(2)H.O(6)) > v (O(6)H.O(3′)), and further impact in crystallite size of CNFs. With the increase of the autohydrolysis strength, the crystallite size of CNFs on the 1 1 ¯ 0 crystal plane could be reduced by up to 26%, but the crystallite size on the 110 crystal plane and the 200 crystal plane increased by 24.5% and 22.6%, respectively. These microstructure changes finally increased the crystallinity index of CNFs, and then resulted in their high thermal stability.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Journal of Soil Science and Plant Nutrition
·
2024
·
IF 3.4
Ullah Saif,Liao Chengrui,Xu Yuanyuan,Li Wannian,Ali Izhar,Han Xiaomei,Ye Shaoming,Yang Mei
Purpose: This study explores the accumulation of phenolic acids in soil within monoculture plantations of Eucalyptus , Acacia mangium , contrasting with mixed species plantations containing both species, across various seasons. The research aims to provide insights into how different plantation types and species compositions influence the presence and levels of phenolic acids in soil. Methods: Soil phenolic acid concentrations were determined using HPLC, analyzing seven phenolic acids, including p-hydroxybenzoic, ferulic, coumaric, and benzoic acids. The kinetic adsorption experiments evaluated phenolic acid adsorption rates and quantities across various soil types. An adsorption kinetic model compared these concentrations between monoculture and mixed forest soils. Results: Our findings showed that plantation types, soil positions and seasons significantly impact phenolic acid accumulation. Non-rhizosphere soil in monoculture Eucalyptus plantations exhibited the highest phenolic acid concentration an average (32 µg g -1 ) across all seasons compared to mixed species plantations. Conversely, the rhizosphere soil of monoculture Acacia mangium displayed the highest content, reaching 71 µg g -1 in March. Notably, four phenolic acids (p-hydroxybenzoic, ferulic, coumaric, and benzoic acids) varied significantly between monoculture and mixed forests. Additionally, adsorption kinetic studies revealed that monoculture Eucalyptus and Acacia mangium soils had higher adsorption capacity compared to mixed species soils. The application of Elovich model yielded the best fit for ferulic and coumaric acids (R 2 > 0.45). Conclusion: Mixed species plantations of Eucalyptus and Acacia mangium significantly influence soil phenolic acid levels compared to monoculture forests and induce alterations in soil adsorption characteristics for phenolic acids, potentially impacting soil fertility and productivity.
研究领域:
生态学
·
土壤学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Xiaoxiao Wei,Yi Liu,Yadan Luo,Zheng Shen,Shuangfei Wang,Mingfu Li,Liming Zhang
In this study, three lignin fractions F1, F2, and F3 were obtained from eucalyptus kraft lignin (EKL) by solvent extraction with ethyl acetate, acetone, and acetone/water. The antioxidant activity of these lignin fractions were measured and evaluated by using the DPPH radical scavenging activity, ABTS radical cation scavenging activity and ferric reducing antioxidant power analysis. These lignin fractions were also characterized by applying Gel permeation chromatography , Fourier transform infrared, 31 P NMR and 2D HSQC NMR techniques. The three different lignin fractions had rather different average molecular sizes, as well as different phenolic and methoxy functional contents. The ethyl acetate fraction (F1) with the lowest average molecular weight (2342 g/mol) and the highest phenolic hydroxyl content (4.2457 mmol/g) and methoxy groups (6.2714 mmol/g) showed high homogeneity and the highest antioxidant activity. Its DPPH scavenging activity, ABTS + scavenging activity, and ferric reducing the antioxidant power were 68.67%, 75.57%, and 91.89 μmol/g, respectively. Moreover, the antioxidant activity of F1 and F2 was found to be higher than that of butylated hydroxytoluene. Therefore, solvent extraction was shown to be an effective way to separate lignin fractions with high homogeneity, high antioxidant activity, which could lead to application of lignin with higher value.
研究领域:
植物化学
·
林产化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
WOOD SCIENCE AND TECHNOLOGY
·
2018
·Vol. 53
·期 1
·177-189
·
IF 1.706
Liu Chao,Li Youming,Hou Yi
Three basic ionic liquids (BILs), 1,3-dimethyl-imidazole dimethyl phosphate([MMIM][DMP]), 1-butyl-3methylimidazolium acetate([BMIM][Ac]) and 1-ethyl-3-methylimidazolium acetate([EMIM][Ac]), were applied to the pretreatment of eucalyptus biomass under microwave conditions with the decreasing alkalinity order of [EMIM][Ac] > [BMIM][Ac] > [MMIM][DMP]. The results showed that the alkalinity of ionic liquids has great effects on the pretreatment of biomass; the greater the alkalinity is, the more capable are the ionic liquids to dissolve hemicellulose, and [MMIM][DMP] with moderately weak alkalinity has a uniquely stronger ability to remove lignin from biomass. Dissolved lignin could easily be separated by using the acetone/water (1:1, v/v), and [MMIM][DMP] pretreatment can extract 41.8% of the total amount of eucalyptus lignin, of which [BMIM][Ac] and [EMIM][Ac] recovered 18.2% and 10.7% of the total amount of raw lignin, respectively. The untreated and ILs-treated eucalyptus samples were characterized by FTIR, XRD, TGA and SEM. Lower thermal stability and changes in crystal structure were observed after pretreatments.
研究领域:
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Polymers
·
2022
·Vol. 14
·期 7
·1333
·
IF 4.967
Jiatian Zhu,Yuqi Bao,Luxiong Lv,Fanyan Zeng,Dasong Du,Chen Liang,Jiayan Ge,Shuangfei Wang,Shuangquan Yao
The preparation of bio-oil through biomass pyrolysis is promoted by different demineralization processes to remove alkali and alkaline earth metal elements (AAEMs). In this study, the hydrothermal pretreatment demineralization was optimized by the response surface method. The pretreatment temperature, time and pH were the response elements, and the total dissolution rates of potassium, calcium and magnesium were the response values. The interactions of response factors for AAEMs removal were analyzed. The interaction between temperature and time was significant. The optimal AAEMs removal process was obtained with a reaction temperature of 172.98 °C, time of 59.77 min, and pH of 3.01. The optimal dissolution rate of AAEMs was 47.59%. The thermal stability of eucalyptus with and without pretreatment was analyzed by TGA. The hydrothermal pretreatment samples exhibit higher thermostability. The composition and distribution of pyrolysis products of different samples were analyzed by Py-GC/MS. The results showed that the content of sugars and high-quality bio-oil (C6, C7, C8 and C9) were 60.74% and 80.99%, respectively, by hydrothermal pretreatment. These results show that the removal of AAEMs through hydrothermal pretreatment not only improves the yield of bio-oil, but also improves the quality of bio-oil and promotes an upgrade in the quality of bio-oil.Keywords:eucalyptus;demineralization;hydrothermal pretreatment;thermostability;pyrolysis products
研究领域:
能源植物学
·
生物质能工程
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）