FITOTERAPIA
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2025
·
IF 2.6
Wenyuan Li,Lin Chen,Minpeng Li,Kaiye Peng,Xuemei Lin,Yifan Feng,Yun Zou,Xia Wu
In this study, the chemical constituents of branch bark and root bark of Morus alba L. were first qualitatively analyzed by UPLC-Q/TOF-MS. A total of 40 compounds were identified, including 20 flavonoids, 3 stilbenes, 1 phenolic acid, 1 alkaloid, 5 arylbenzofurans and 10 Diels-Alder adducts. Of these, 19 compounds were specific to the root bark, 10 to the branch bark, and 11 were common to both. Total phenolic and flavonoid contents were determined, and anti-inflammatory activities were evaluated. Ethyl acetate extracts of both significantly inhibited NO secretion, with the root bark showing a stronger effect than the branch bark. Chemical components of branch bark were further separated, and the anti-inflammatory activities of these isolated compounds were investigated. Eight compounds were isolated from the ethyl acetate extract of the branch bark of Morus alba L. . These compounds were able to inhibit the release of inflammatory factors, including NO, IL-6, and IL-1β, in a concentration-dependent manner. Among them, compounds SZP-1, SZP-6 and SZP-8 exhibited superior in vitro anti-inflammatory activity, comparable to the positive drug meloxicam at high concentrations. In addition, the quality standard of the branch bark of Morus alba L. was established for the first time according to the 2020 edition of the Chinese Pharmacopoeia. This study provides valuable insights into the medicinal value of the branch bark of Morus alba L. and promotes the comprehensive utilization of mulberry branch resources.
研究领域:
药理学
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植物化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Plants-Basel
·
2025
·Vol. 14
·期 7
·1126
·
IF 4.1
Haonan Li,Michael Ackah,Frank Kwarteng Amoako,Aaron Tettey Asare,Jianbin Li,Zhenjiang Wang,Qiang Lin,Changyu Qiu,Mengdi Zhao,Weiguo Zhao
Vegetative propagation of mulberry (Morus albaL.) via sapling methods, due to the ability to exponentially multiply lateral buds on stem cuttings to enhance rapid shoot formation, is crucial for sericulture industries. The sprouting of mulberry using stubbles is an emerging method for rapid and mass production of mulberry leaves, but the growth mechanisms associated with its use remain obscure. This study is the first to report how the differential stubble lengths from mulberry plants alter and modulate phytohormones and the associated mechanisms. This study seeks to evaluate the growth mechanisms by elucidating the phytohormone signature modulation in response to differential stubble lengths of 0 cm, 5 cm, 10 cm, 20 cm, and a control via targeted metabolomics analysis in mulberry leaves. The results consistently show that the use of differential stubble lengths of mulberry promoted growth, the number of buds, aboveground biomass, and branch and leaf weights by improving the net photosynthesis, transpiration rate, stomatal conductance, and intercellular CO2relative to the control. The differential stubble lengths not only caused contrasting responses in the contents of plant hormones, including salicylic acid (SA), abscisic acid (ABA), indole-3-acetic acid (IAA), jasmonic acid (JA), and gibberellin (GA), but also modulated higher elemental contents relative to the control. The results further reveal significant and positive correlations between the phytohormones and all growth, biomass, and photosynthetic parameters, highlighting the role of phytohormones in the sprouting and rejuvenation of mulberry stubbles. Meanwhile, the targeted metabolomics analysis identified a total of 11 differentially accumulated phytohormones in response to the differential stubble lengths, which were significantly implicated and enriched in three major pathways, including the biosynthesis of plant hormones (ko01070), metabolic pathways (ko01100), and the plant hormone signal transduction pathway (ko04575). The use of stubbles for rapid leaf production in mulberry plants is of great importance to improve early sprouting and cutting survival, as well as shortening growth and rooting time, and is highly recommended for the sericulture industries.
研究领域:
植物学
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代谢组学
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农学
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DOI
PubMed
高影响因子
MOLECULES
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2018
·Vol. 23
·期 3
·554
·
IF 3.098
Qianqian Liang,Qiuying Wang,Yuan Wang,Ya-nan Wang,Jia Hao,Miaomiao Jiang
The primary metabolites in aqueous extract of mulberry (Morus albaL.) leaves were characterized by using proton nuclear magnetic resonance (1H-NMR) spectroscopy. With the convenience of resonance assignment, GABA together with the other 10 primary metabolites was simultaneously identified and quantified in one1H-NMR spectrum. In this study, external calibration curves for metabolites were employed to calculate the concentrations of interests. The proposed quantitative approach was demonstrated with good linearity (r2ranged in the interval of 0.9965–0.9999), precision, repeatability, stability (RSD values in the ranges of 0.35–4.89%, 0.77–7.13% and 0.28–2.33%, respectively) and accuracy (recovery rates from 89.2% to 118.5%). The established1H-NMR method was then successfully applied to quantify 11 primary metabolites in mulberry leaves from different geographical regions within a rapid analysis time and a simple sample preparation procedure.Keywords:quantitative1H-NMR;Morus alba;amino acids;saccharides;organic acids
研究领域:
分析化学
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植物科学
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代谢组学
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DOI
PubMed
高影响因子
Metabolites
·
2022
·Vol. 12
·期 2
·106
·
IF 5.581
Cui Wu,Huijun Wang,Zhenying Liu,Bo Xu,Zhuojun Li,Pingping Song,Zhimao Chao
The differences in metabolites in male and female individuals of dioeciousMorus albaL. (Moraceae) are usually ignored and lack study. In the present study, 58 leaves and 61 stem barks from male and female individuals were analyzed by untargeted metabolomics via headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) coupled with chemometrics, including principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA). A total of 66 and 44 metabolites were identified from leaves and stem barks, respectively. Four and eight differential metabolites among candidate metabolites in leaves and stem barks from male and female individuals were identified. Moreover, females possessed stronger antioxidant activity than males. This is the first report where untargeted metabolomics coupled with chemometrics was used to analyze the different metabolites and to discriminate the gender of leaves and stem barks of dioeciousM. alba. It provided the basis for further study ofM. albaand reference value for researching dioecious plants.Keywords:Morus albaL.;dioecious plant;leaves;stem barks;untargeted metabolomics;chemometrics
研究领域:
植物学
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代谢组学
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药用植物研究
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DOI
PubMed
高影响因子
Plants-Basel
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2024
·Vol. 13
·期 4
·482
·
IF 4
Ping Li,Siyi Wang,Mengyuan Liu,Xue Dai,Huicong Shi,Weihong Zhou,Sheng Sheng,Fuan Wu
Bacterial wilt is a significant soil-borne disease that poses a threat to mulberry production yield and quality of agricultural production worldwide. However, the disease resistance mechanisms dependent on root exudates are not well understood. In this present study, we investigated the antibacterial mechanisms of the main active substances (erucamide, oleamide, and camphor bromide) present in mulberry root exudates (MRE) againstRalstonia pseudosolanacearum(Rp), the causal agent of bacterial wilt. Our findings revealed that these three active substances inhibited the growth activity ofRpby affecting the cell morphology and extracellular polysaccharide content, as well as triggering a burst of reactive oxygen species. The active substances induced oxidative stress, leading to a decrease inRpgrowth. Additionally, the expression levels of key genes in the hrp gene cluster (hrpB,hrpX, andhrpF) and other virulence-related genes (such asripAW,ripAE,Rs5-4819,Rs5-4374,ace,egl3, andpehB) were significantly reduced upon treatment with the active substances. Further pathogenicity experiments demonstrated that root exudates (at a concentration of 1.5 mg·mL−1) delayed or slowed down the occurrence of bacterial wilt in mulberry. These findings provide valuable insight into the antimicrobial mechanisms of MRE againstRpand lay a theoretical foundation for the development and application of biocontrol agents to control mulberry bacterial wilt.
研究领域:
植物病理学
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微生物学
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DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2025
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IF 4
Siye Zhou,Jiayun Li,Dongping Chen,Zhixuan Xu,Kudousi Kuerban,Qiang Liu,Muhammad Irfan Waris,Wenwu Wu,Nannan Lv,Jianting Fan
Hyphantria cunea , a globally significant quarantine pest, causes severe ecological and economic impacts in invaded regions. Attractants are environment-friendly and valuable pest management tools for H. cunea control. In this study, we identified that the combination of β-ocimene with sex pheromone components was strongly attractive to H. cunea males. This attractant combination activates the Ras signaling pathway and stimulates Ca 2+ in male adults following exposure. Feeding and oviposition behavior results demonstrated that H. cunea prefers Morus alba over Platanus × acerifolia , Metasequoia glyptostroboides , and Taxodium distichum . In the fresh branches of M. alba with leaves, the relative abundance of β-ocimene (36.33 ± 0.41 %) was significantly higher than the other hosts. Electroantennography recordings, behavioral choice assays, and field trapping trials showed that the combination of β-ocimene with sex pheromone components significantly enhanced male response and attraction. The number of trapped males using β-ocimene/ sex pheromone blend was 3.7-fold higher than that of sex pheromone or β-ocimene alone in the field, indicating a synergistic effect of adding β-ocimene to sex pheromone. The molecular mechanism of β-ocimene/sex pheromone synergism was further analyzed. After exposure to the attractant, the Ras signaling pathway in the heads of males are activated ( HcILP and HcPLCε1 are upregulated, while HcRasGAP and HcPLD are downregulated), which further stimulates the expression of IP3 and Ca 2+ . The activation of Ca 2+ may be the key reason for its higher attraction to males. These findings provide a theoretical basis for the selection and mechanistic understanding of attractants for H. cunea , offering insights for attractant-based pest control strategies.
研究领域:
昆虫学
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害虫管理
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分子生物学
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生态学
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DOI
PubMed
高影响因子
Heritage Science
·
2018
·Vol. 6
·期 1
·1-14
·
IF 1.58
Wei Le,Chen Wenjia,Jin Gaowa,Guo Zhimou,Wang Yunli,Kang Baoqiang,Wang Na,Gu An,Zhang Yun,Lei Yong
Tie luo (affixed hanging) was an expressive form of traditional Chinese calligraphy or paintings, and popular in the imperial palaces for interior decorations in Qing Dynasty (1644–1911 C.E.). A piece of calligraphic tie luo , written by an eminent calligrapher Gu Gao (1763–1832 C.E.), was restored recently in the Palace Museum, Beijing, China. The paper with pigment coating specially made for this calligraphic piece was named as fenjian . This article presented results from materials identification prior to the restoration. Multianalysis with scientific approaches revealed how tie luo was manufactured. It could be concluded that the investigated tie luo used a paper made from bast fibers of mulberry trees. And the decorative ground layer for the piece was also studied, which indicated that a type of organic red pigments were used for the masterpiece. White lead was considered as the main pigment, mixed with a red dye extracted from sappanwood. Meanwhile, animal glue, drying oils and beeswax were confirmed as the organic binding media. In dye analysis, protosappanin B and brazilin as well as brazilein were identified, which implied that sappanwood was used for the organic manufacture of pigments. In addition, both Nowik type A and C were found in the research, which were characteristic of sappanwood for identification.
研究领域:
文物保护
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材料科学
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历史文物研究
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DOI
高影响因子
CHEMISTRY & BIODIVERSITY
·
2021
·Vol. 19
·期 2
·e202100735
·
IF 2.408
Zhipeng Qi,Xianyu Fan,Chunyi Zhu,Dongsheng Chang,Jianjun Pei,Linguo Zhao
Synthesis of β-ionone in microbial cell factories is limited by the efficiency of carotenoid cleavage dioxygenases (CCDs). To obtain genes responsible for specific cleavage of carotenoids generating β-ionone, a novel carotenoid cleavage dioxygenase 1 from Morus notabilis was cloned and overexpressed in Escherichia coli . The MnCCD1 protein was able to cleave a variety of carotenoids at the positions 9, 10 (9′, 10′) to produce β-ionone, 3-hydroxy-4-oxo-β-ionone, 3-hydroxy-β-ionone, and 3-hydroxy-α-ionone in vitro . MnCCD1 could also cleave lycopene and β-carotene at the 9, 10 (9′, 10′) bind bond to produce pseudoionone and β-ionone, respectively, in E. coli accumulating carotenoids. The enzyme activity of MnCCD1 was reached 2.98 U/mL at optimized conditions (temperature 28 °C, IPTG 0.1 mM, induction time 24 h). The biochemical characterization of MnCCD1 revealed the optimal activities were at pH 8.4 and 35 °C. The addition of 10 % ethanol could increase enzyme activity at above 15 %. However, an obvious decline was observed on enzyme activity as the concentration of Fe 2+ increased (0–1 mM). The V max for β-apo-8′-carotenal was 72.5 U/mg, while the K m was 0.83 mM. The results provide a foundation for developing the application of carotenoid cleavage dioxygenases as biocatalysis and synthetic biology platforms to produce volatile aroma components from carotenoids.
研究领域:
植物生物化学
·
合成生物学
·
酶学
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DOI
PubMed
高影响因子
Food & Function
·
2019
·Vol. 10
·期 10
·6915-6926
·
IF 3.241
Sen Guo,Li Liu,Shanshan Zhang,Chuang Yang,Wenping Yue,Haoan Zhao,Chi-Tang Ho,Junfeng Du,Hai Zhang,Naisheng Bai
Morus species, commonly known as mulberry, is widely distributed in China. The mulberry tree is a high-value plant in agriculture. Morus australis is one of the major Morus species growing in Northern China. However, the biological properties of the main constituents of M. australis roots were not well studied. In the present study, through extensive chromatographic and spectral analysis, 12 phenolic compounds were isolated and identified from the M. australis roots. Compounds 1, 2, 8, 9 and 12 were isolated from M. australis roots for the first time. Antitumor activities of these polyphenols were studied on the A549 cell line. Compounds 1, 5 and 6 exhibited cytotoxicity on A549 cells and induced apoptosis in A549 cells via the intrinsic mitochondrial pathway. They also mediated inhibition of autophagic flux contributed cell death via the PI3k/Akt/mTOR pathway. In order to explore more potential bioactivities of these isolates, α-glucosidase, acetylcholinesterase and tyrosinase inhibitory activities were studied, and the results demonstrated that the inhibitory activity of these polyphenols on enzymes was not defined by their basic structural skeletons, but by the substituted position.
研究领域:
药理学
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生物化学
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植物科学
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癌症研究
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Lin-Lin Tian,Yan-Xue Bi,Hua Zhang
Fifteen stilbenoid derivatives, including five previously undescribed ones (albaphenols A–E, 1–5) with diverse scaffolds, were obtained from the well-known agricultural economic tree Morus alba. Their structures, including absolute stereochemistries, were fully characterized by detailed interpretation of spectroscopic data and quantum chemical computational analyses of nuclear magnetic resonance (NMR) and electric circular dichroism (ECD). Albaphenol A (1) features an unprecedented rearranged carbon skeleton incorporating a novel 2-oxaspiro[bicyclo[3.2.1]octane-6,3′-furan] motif; albaphenol C (3) is likely derived from a cometabolite through an interesting intramolecular transesterification reaction; and albaphenol E (5) bears a cleavage-reconnection scaffold via a dioxane ring. All of the compounds exhibited significant inhibition against the diabetic target α-glucosidase, with low to submicromole IC50 values (0.70–8.27 μM), and the binding modes of selected molecules with the enzyme were further investigated by fluorescence quenching, kinetics, and molecular docking experiments. The antidiabetic effect of the most active and abundant mulberrofuran G (6) was further assessed in vivo in diabetic mice, revealing potent antihyperglycemic activity and comparable antidiabetic efficacy to the clinical drug acarbose.
研究领域:
药理学
·
有机化学
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生物化学
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药物化学
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产品关联
DOI
PubMed
高影响因子
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