RSC Advances
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2021
·Vol. 11
·期 41
·25646-25652
·
IF 3.361
Qian-Hui Shen,Qi Huang,Ju-Ying Xie,Kun Wang,Zheng-Ming Qian,De-Qiang Li
In the present study, a simple and efficient approach based on the online extraction-high performance liquid chromatography coupled with ABTS antioxidant assay (OLE-HPLC-ABTS) was established to quickly and directly analyze the antioxidants in S. baumii. Through this system, the HPLC mobile phase via a guard column packed with a S. baumii sample was used for online extraction (OLE). The separation was performed on an Agilent Poroshell EC-C18 column with a gradient elution using 0.1% formic acid (A) and 0.1% formic acid–acetonitrile (B) as mobile phase systems and detected at a wavelength of 254 nm. Then, the separated compounds were reacted with the antioxidant solution (ABTS), and the response was recorded at a wavelength of 400 nm. The developed analytical method was successfully applied to S. baumii samples, and eight antioxidants were identified. The established system integrated the online extraction, separation and online antioxidant detection, which is rapid, efficient, and suitable for the rapid screening of antioxidant compounds from solid sample mixtures.
研究领域:
分析化学
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药用真菌学
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产品关联
DOI
PubMed
高影响因子
BIOORGANIC CHEMISTRY
·
2023
·
IF 5.1
Jiling Song,Zhanwei Wang,Yu Chi,Yong Zhang,Chenyi Fang,Yuting Shu,Jing Cui,Helong Bai,Jing Wang
Xanthine oxidase (XO) plays a critical role in the progression of gout. We showed in a previous study that Sanghuangporus vaninii ( S. vaninii ), a perennial, medicinal, and edible fungus traditionally used to treat various symptoms, contains XO inhibitors. In the current study, we isolated an active component of S. vaninii using high performance countercurrent chromatography and identified it as davallialactone using mass spectrometry with 97.726 % purity. A microplate reader showed that davallialactone had mixed inhibition of XO activity with a half-inhibitory concentration value of 90.07 ± 2.12 μM. In addition, the collision between davallialactone and XO led to fluorescence quenching and conformational changes in XO, which were mainly driven by hydrophobicity and hydrogen bonding. Molecular simulations further showed that davallialactone was located at the center of the molybdopterin (Mo-Pt) of XO and interacted with amino acid residues Phe798, Arg912, Met1038, Ala1078, Ala1079, Gln1194, and Gly1260, suggesting that entering the enzyme-catalyzed reaction was unfavorable for the substrate. We also observed face-to-face π-π interactions between the aryl ring of davallialactone and Phe914. Cell biology experiments indicated that davallialactone reduced the expression of the inflammatory factors, tumor necrosis factor alpha and interleukin-1 beta ( P < 0.05), can effectively alleviate cellular oxidative stress. This study showed that davallialactone significantly inhibits XO and has the potential to be developed into a novel medicine to prevent hyperuricemia and treat gout.
研究领域:
药理学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2020
·
IF 3.067
Jing Huang,Kun Wang,Song Zuo,Lei Chen,Zhongyang Ding,Mohamed El-shazly,Bo-Bo Zhang
Sanghuangporus species are precious edible and medicinal fungi that are traditionally used in Asian countries. Triterpenoids, the major bioactive constituents of Sanghuangporus spp. are currently receiving a lot of attention from the scientific community due to their potent pharmacological activities. However, very few reports have been conducted about the possibility of adjusting the fermentation conditions to increase the triterpenoids yield from certain fungi such as Sanghuangporus baumii . In this study, we examined the effects of four different extracts from mulberry shavings on the production of triterpenoids and traced the major components from the effective extracts by GC-MS to provide a reference for further exploration of the triterpenoids synthetic mechanism. It was found that unsaturated fatty acids significantly promoted the production of triterpenoids. The results suggested a direct correlation between the degree of unsaturation of fatty acid and the triterpenoids yield. The best stimulatory additive was linolenic acid . It increased the triterpenoids yield to 573 mg/L, which was 2.53 times higher than the control. This study not only showed that addition of mulberry shavings extract was an efficient fermentation protocol to improve the triterpenoids yield of S. baumii , but it also highlighted the importance of fatty acid content in the production of triterpenoids.
研究领域:
微生物学
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药理学
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生物化学
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DOI
高影响因子
Genes
·
2025
·Vol. 16
·期 2
·184
·
IF 2.8
Ruipeng Liu,Tingting Sun,Pengyu Du,Zengcai Liu,Yawei Li,Xinyu Tong,Li Zou
Background: Basic helix–loop–helix (bHLH) transcription factors (TFs) widely exist in eukaryotic organisms and play a key role in plant growth and development in response to environmental stresses.Sanghuangporus baumii, an important medicinal mushroom known for its anticancer properties, has limited research on the bHLH gene family. Methods: This research utilized the genomic data fromS. baumiito identify bHLH family members, and their gene structure, conserved motifs, and phylogenetic relationship were characterized. Additionally, we conducted an analysis of promoter cis-elements and predicted protein interaction networks. We also examined the expression profiles of bHLH genes during different developmental stages and in response to four abiotic stresses: heat, cold, oxidative stress, and heavy metal exposure. Finally, we overexpressed the candidate geneSbbHLH3in yeast to assess its tolerance to these different stress conditions. Results: A total of 12SbbHLHgenes were identified inS. baumii, and the members of the bHLH gene family displayed a variety of physicochemical characteristics, reflecting their diverse array of functions. Based on homology, the SbbHLH proteins are more closely related to those found inLentinula edodesandPleurotus ostreatus. The analysis of promoter cis-elements showed thatSbbHLHs contain several elements associated with abiotic stress response, and a network prediction identified 28 bHLH-interacting proteins. Expression pattern analysis revealed that mostSbbHLHgenes exhibited a positive response to different developmental stages and abiotic stresses. Notably, the overexpression ofSbbHLH3significantly enhanced stress tolerance in yeast. Conclusions: This study provides a comprehensive assessment of the bHLH family inS. baumii, delivering new genetic resources for breeding resistant varieties.
研究领域:
真菌学
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分子生物学
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遗传学
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DOI
PubMed
高影响因子
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
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2021
·Vol. 69
·期 6
·2561-2572
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IF 2.431
Yongjun Xia,Caiyun Yang,Xiaofeng Liu,Guangqiang Wang,Zhiqiang Xiong,Xin Song,Yijin Yang,Hui Zhang,Lianzhong Ai
There have been many studies on the activities and polysaccharide production of Sanghuangporus vaninii . However, few studies have looked at triterpene production from S. vaninii using liquid-state fermentation. A method for enhancing the production of triterpenes by in situ extractive fermentation (ISEF) was studied. Eight solvents were investigated as extractants for triterpene production in the ISEF system. The results showed that using vegetable oil as an extractant significantly increased the yield of total triterpenes and biomass of S. vaninii YC-1, reaching 18.98 ± 0.71 and 44.67 ± 2.21 g/L, respectively. In 5 L fermenter experiments, the added vegetable oil improved the dissolved oxygen condition of the fermentation broth and promoted the growth of S. vaninii YC-1. Furthermore, adding vegetable oil increased the expression of fatty acid synthesis-related genes such as FAD2 and SCD , thereby increasing the synthesis of unsaturated fatty acids in the cell membrane of S. vaninii YC-1. Therefore, the cell membrane permeability of S. vaninii YC-1 increased by 19%. Our results indicated that vegetable oil increased the permeability of S. vaninii YC-1 cell membranes to promote the production of total triterpenes. The use of vegetable oil as an extractant was thus effective in increasing the yield of triterpenes in the ISEF system.
研究领域:
微生物学
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生物技术
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药用真菌研究
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DOI
PubMed
高影响因子
Fermentation-Basel
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2023
·Vol. 9
·期 3
·308
·
IF 3.7
Tian Li,Yuxia Mei,Ji Li,Wendi Yang,Fanfan He,Jiaxin Ge,Fei Chen,Yicheng Yang,Aowen Xie,Yangyang Liu,Yunxiang Liang
Sanghuang, a traditional Chinese medicinal herb obtained from numerous related fungal species in the genusSanghuangporus, contains many bioactive substances that display a variety of beneficial pharmacological activities, including antioxidant, antitumor, and antidiabetic. We collected wild fruiting bodies from various Chinese localities, obtained nine pure sanghuang strains (termed S1 to S9), cultured the strains by solid-state fermentation, extracted and purified sanghuang flavonoids (termed SHFs) from mycelia, and analyzed their antioxidant abilities and α-amylase inhibitory (α-AI) activities. SHFs from strains S2, S6, S7, and S9 displayed strong DPPH radical scavenging abilities and iron reducing abilities, while SHFs from S1, S3, S5, and S8 had strong α-AI activities. SHF components were analyzed by HPLC in combination with a Chinese medicine fingerprint similarity evaluation system and statistical analyses. SHFs from the nine strains showed high fingerprint similarity. Fifteen peaks in the chromatograms (termed 1–15) were subjected to cluster analysis, which revealed that differences in SHF composition were related to geographic origin and host species. The strains with strong antioxidant activities had relatively large peak 5 and peak 9 areas, while those with strong α-AI activities had relatively large peak 13 areas. Such variation in SHF activities is attributable to differences in their components. Our findings indicate that careful selection of SHFs based on these activities will strengthen their potential development as antioxidant and antidiabetic agents.Keywords:sanghuang;solid-state fermentation;flavonoids;antioxidant activity;α-amylase inhibitory activity
研究领域:
中药学
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生物化学
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药理学
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产品关联
DOI
高影响因子
JOURNAL OF FOOD SCIENCE
·
2022
·Vol. 87
·期 4
·1575-1585
·
IF 3.693
Wei Zhang,Yunjing Luo,Ziqi Xie,Chenchen Kong,Zhen Na
In this study, morin magnetic molecularly imprinted polymers (Morin-MMIPs) were synthesized based on magnetic nanoparticles and surface molecularly imprinted technology with superparamagnetism and extraction selectivity. The polymers allowed the separating of morin from complex matrices in the presence of an external magnetic field with no need for centrifugation or filtration. The microstructure of the polymers was characterized by scanning electron microscopy and transmission electron microscopy. Meanwhile, the functional group and magnetic properties of the polymers were characterized using Fourier transform infrared spectroscopy (FT-IR) and magnetic vibration meter (VSM). The maximum adsorption capacity of MMIPs was 3.24 mg/g, which was 2.55 times higher than that of MNIPs (1.27 mg/g). Morin was quantified by HPLC-DAD, which showed good linearity in the concentration range of 0.05–60 µg/ml with the correlation coefficient R 2 = 0.9993. The limit of detection (LOD) was 0.08 µg/ml, and the spiked recoveries were 87.5–106.8%. The calculation of the adsorption isotherm and kinetic model revealed the adsorption mechanisms, and the adsorption process was consistent with the Langmuir adsorption isotherm and pseudo-secondary kinetic models. Likewise, the material has been successfully used to extract and separate morin from food samples. The method reported in this paper has the advantages of fast adsorption speed, high selectivity, and environmental friendliness. It provided a reliable method for the separation and detection of morin or other natural products.
研究领域:
分析化学
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生物技术
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药学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
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2024
·Vol. 29
·期 19
·4564
·
IF 4.2
Yanglan Luo,Naixin Cao,Liling Huang,Lanlan Tang,Xuzhou Liu,Wenlong Zhang,Shilv Huang,Xiuchao Xie,Yong Yan
Sanghuang as a medicinal fungus in China has a history of more than 2000 years, and is known as the “forest gold”. Most notably, the polysaccharides of Sanghuangporus sp. have attracted widespread attention due to their significant bioactivity in recent years. At present, extensive studies are being carried out on the extraction methods, structural characterization, and activity evaluation of polysaccharides. Here, we aimed to evaluate the structure and bioactivity of LEPS-1, an exopolysaccharide derived from the S. sanghuang JM-1 strain. The structure was elucidated by chromatography/spectral methods and hydrolyzation, and the solubility, the antioxidant activity, hypoglycemic activity and immunomodulatory activity were investigated. Results showed that LEPS-1 contained a →2)-α-Manp(1→6)-α-Galp(1→[2)-α-Manp(1→] n →2,6)-α-Manp(1→6,2)-α-Manp(1→3)-α-Manp(1→ backbone substituted at the O -6 and O -2 positions with side chains. These two branching fragments were β-Manp(1→. The molecular weight of LEPS-1 is 36.131 kDa. The results of biological activity analysis suggested that LEPS-1 was easily soluble in water, with reducing capability and DPPH radical scavenging capability. Furthermore, the IC 50 values of LEPS-1 against α-amylase and α-glucosidase were 0.96 mg/mL and 1.92 mg/mL. LEPS-1 stimulated RAW264.7 cells to release NO, TNF-α and IL-6 with no cytotoxicity, showing potent potential for immunomodulatory activity. These findings describe a potential natural exopolysaccharide with medicinal value and a basis for the development of S. sanghuang exopolysaccharides.
研究领域:
药物化学
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药理学
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生物化学
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真菌学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Lihong Zhang,Shuang Zhao,Jingxuan Wang,Jiaxin Zhang,Tian Zheng,Jingyi Li,Chuangui Ma,Jingxin Liu
Polysaccharides are of interest due to their multiple biological activities. However, the molecular weight of natural polysaccharides is excessively high, resulting in low bioavailability. In this study, β-glucanase was used to degrade the polysaccharides of Sanghuang vaninii , and the hydroxyl radical scavenging rate was used as an indicator to optimize the enzymatic process. The monosaccharide composition, molecular weight, thermal stability, infrared spectra, and microstructure of Sanghuang vaninii polysaccharides (SVP) were characterized before and after enzymatic degradation under optimal conditions. Their properties, including antioxidant, hypoglycemic, and anti-inflammatory effects, were also measured. The findings indicated that for SVP, the optimal enzyme digestion conditions were an enzyme activity of 63 U/mL, a digestion time of 3.5 h, a pH value of 5.5, and a temperature of 50 °C. After undergoing enzymatic digestion, the SVP exhibited a hydroxyl radical scavenging rate of 87.3 %. No significant changes were observed in the monosaccharide composition and basic structure before and after enzymatic hydrolysis; however, the molecular weight decreased from 7.473 × 10 4 Da to 5.533 × 10 3 Da, and the original smooth lamellar structure was disrupted. In addition, the antioxidant, hypoglycemic, and anti-inflammatory activities of SVP were significantly enhanced after enzymatic digestion. This study provides a reference for the industrialization and activity research of SVP.
研究领域:
食品科学
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生物化学
·
药理学
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微生物学
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生物技术
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Xing Luo,Ji-Hang Jiang,Shi-Liang Liu,Jian-Yun Gao,Li-Wei Zhou
Rice, a primary staple food, may be improved in value via fermentation. Here, ten medicinal basidiomycetous fungi were separately applied for rice fermentation. After preliminary screening, Ganoderma boninense , Phylloporia pulla , Sanghuangporus sanghuang and Sanghuangporus weigelae were selected for further LC-MS based determination of the changes in metabolic profile after their fermentation with rice, and a total of 261, 296, 312, and 355 differential compounds were identified, respectively. Most of these compounds were up-regulated and involved in the metabolic pathways of amino acid metabolism, lipid metabolism, carbohydrate metabolism and the biosynthesis of other secondary metabolites. Sanghuangporus weigelae endowed the rice with the highest nutritional and bioactive values. The metabolic network of the identified differential compounds in rice fermented by S. weigelae illustrated their close relationships. In summary, this study provides insights into the preparation and application of potential functional food via the fermentation of rice with medicinal fungi.
研究领域:
食品科学
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微生物学
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生物化学
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营养学
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DOI
PubMed
高影响因子
Current Analytical Chemistry
·
2024
·Vol. 20
·期 4
·264-274
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IF 1.7
Qu Yunhe,Zhang Pingping,Cui Jing,Ni Xiuzhen,Song Kai,Shi Dongfang
Introduction: In this study, we optimized the extraction process, analyzed the structure and assessed the antioxidant activity of Sanghuangporus baumii polysaccharide (SBP). The present results provide important information for the Sanghuangporus baumii polysaccharides
in potential natural antioxidant effects. Background: The extraction and structural analysis of polysaccharides from Sanghuangporus has gained significant attention in the fields of chemistry, medicine, and life sciences. There is great significance in maximizing the extraction
of polysaccharides from Sanghuangporus and developing their potential products in a scientific and rational manner. Objective: The study was designed to establish an efficient and practical extraction process for SBP, and then investigated the structure and the antioxidant activity.
Methods: The Response Surface Methodology (RSM) based on Box-Behnken design was used to explore the ultrasound-assisted extraction of SBP, and the structure of SBP was studied by ultraviolet spectroscopy, infrared spectroscopy and other instrumental analysis methods. The total antioxidant
capacity of SBP was studied by the Ferric Reducing Ability of Plasma (FRAP) method, and the scavenging capacity of ABTS+·, DPPH· and OH· was carried out as the index to investigate its antioxidant activity in vitro. Results: The statistical analysis
results showed that the optimal conditions for extracting SBP were an ultrasound time of 20.74 min, ultrasound power of 268.40 W and material-liquid ratio of 1:25.71. Under optimal conditions, the experimental yield of SBP was 3.36 ± 0.01%. The RSM optimization process was applied to
the experiment of complex enzyme extraction of SBP, and the yield increased to 4.72 ± 0.03%. Structural analysis showed that SBP mainly consisted of glucose, a small amount of mannose and galactose, and the molecular weight distribution was uneven, mainly concentrating in the three
parts of 24.5,6.4,2.5 kDa. Moreover, SBP exhibited dose-dependent and strong reducing power and radical scavenging activity. For DPPH·, ABTS+· and OH· radical scavenging assays, IC50 values were 1.505 ± 0.050 mg/ml, 0.065 ± 0.036 mg/ml
and 0.442 ± 0.007 mg/ml, respectively. Conclusion: In the present study, a β-linked heteroglucan (SBP) was extracted using the optimized process combining enzymes from the fruiting bodies of Sanghuangporus baumii. SBP exhibited effective and dose-dependent antioxidant
activities. Our findings were of great value in terms of developing polysaccharides with potential natural antioxidants.
研究领域:
化学
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医学
·
生命科学
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药理学
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生物化学
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DOI
高影响因子
Frontiers in Microbiology
·
2022
·
IF 6.064
Huo Jinxi,Sun Yuqing,Pan Meiliang,Ma Huanyan,Lin Tianbao,Lv Zhiqiang,Li Yougui,Zhong Shi
Sanghuangprous vaninii is a wood-inhabiting fungus, and its mycelium and fruiting body show excellent medicinal values. Mulberry is one of the major hosts of S. vaninii, however, the mechanism of mulberry affecting the growth of S. vaninii has not been reported. In the present study, a mulberry-inhabiting strain of S. vaninii was selected to explore the effects of mulberry branch extracts (MBE) on the growth of the strain. Results showed that MBE could significantly promote the growth of S. vaninii mycelium at the concentration of 0.2 g/l. After 16 days of liquid culture, the dry weight of mycelium in 0.2 g/l MBE medium was higher by three times compared with that in the control. The non-targeted metabonomic analysis of the culture medium at different culture times and concentrations was conducted to find the key components in MBE that promoted the growth of S. vaninii mycelium. Under the different concentrations of MBE culture for 10 and 16 days, 22 shared differential metabolites were identified. Next, in accordance with the peak value trend of these metabolites, HPLC–MS and liquid culture validation, four components derived from MBE (i.e., scopoletin, kynurenic acid, 3,5-dihydroxybenzoic acid and 2,4-dihydroxybenzoic acid) could significantly increase the growth rate of mycelium at the concentration of 2 mg/l. Transcriptomic and qRT-PCR analyzes showed that MBE could upregulate hydrolase-related genes, such as serine–glycine–asparaginate–histidine (SGNH) hydrolase, alpha-amylase, poly-beta-hydroxybutyrate (PHB) depolymerase, glycosyl hydrolase family 61, cerato-platanin protein and Fet3, which might enhance the nutrient absorption ability of S. vaninii. Importantly, MBE could significantly increase the content of harmine, androstenedione and vesamicol, which have been reported to possess various medicinal effects. Results suggested that MBE could be an excellent additive for liquid culture of S. vaninii mycelium, and these hydrolase-related genes also provided candidate genes for improving the nutrient absorption capacity of S. vaninii.
研究领域:
真菌学
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代谢组学
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转录组学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）