FUNGAL GENETICS AND BIOLOGY
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2022
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IF 3.883
Jiazhen Qin,Jianwen Teng,Zhongyu Li,Ning Xia,Baoyao Wei,Li Huang
Similar to Pu-erh tea, Liupao tea is a post-fermented tea that is produced through natural fermentation by microorganisms. Penicillium citrinum is involved in multiple production processes of Liupao tea that can produce citrinin, a secondary metabolite with renal toxicity; however, the effect of P. citrinum on the quality of Liupao tea has not been investigated yet. Citrinin production is regulated by approximately 16 biosynthesis genes. However, little is known about the genetic background of citrinin in the complex Liupao tea system. In the present study, we cultured P. citrinum on potato dextrose agar and Liupao tea powder media and analyzed the changes of its nutritional components in Liupao tea. We selected six citrinin biosynthesis genes identified in Monascus exhibiting homology and high sequence similarity to those in P. citrinum and further analyzed the expression of citrinin biosynthesis genes in Liupao tea and the changes in citrinin yield. The results showed that the changes in nutritional components of Liupao tea were closely related to the growth and metabolism of P. citrinum and the quality of the tea. Decreases in the contents of soluble sugars (from 10.29% to 9.58%), soluble pectins (from 3.71% to 3.13%), free amino acids (from 3.84% to 3.14%), and tea polyphenols (from 22.84% to 18.78%) were noted. The Spearman’s correlation analysis indicated that P. citrinum growth can improve the tea quality to some extent. Quantitative real-time PCR demonstrated that ctnA gene was a positive regulator of citrinin production regardless of the culture medium used. ctnA and orf5 expressions greatly influenced the metabolism of citrinin by P. citrinum in Liupao tea. In conclusion, the citrinin biosynthesis genes, ctnA and orf5 , may be the promising targets for developing strategies to control P. citrinum infection and citrinin biosynthesis in Liupao tea.
研究领域:
食品科学
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微生物学
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分子生物学
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制茶技术
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DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2020
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IF 4.006
Shanshan He,Xin Liu,Yanling Wang,Jianhua Xie,Heng Gao,Xiujiang Li,Zhibing Huang
Monascus mold produces useful bioactive compounds such as Monascus pigments, monacolin K, and ergosterol . In this study, metabolomics analysis was used to investigate the metabolic profile changes in Monascus aurantiacus Li AS3.4384 (MALA) induced by genistein at different fermentation times using UHPLC-Q-TOF-MS/MS. The addition of genistein was found to not only reduce citrinin but also suppress significant differential metabolites. A total of 21 significant differential metabolites were involved in the most significant metabolic pathways (impact >0.1). These metabolites affected the aspartic acid , glutamic acid pathway, tricarboxylic acid (TCA) cycle, pyruvate synthesis pathway, and lysine degradation pathway. Thus, the addition of genistein may change the precursor for citrinin synthesis of Monascus . These findings provide significant insight into the Monascus metabolic pathways affected by genistein and will help to improve the quality control and safety of Monascus in food and supplements.
研究领域:
食品科学
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微生物学
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代谢组学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2019
·Vol. 99
·期 10
·4772-4780
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IF 2.422
Zhibing Huang,Lijuan Zhang,Heng Gao,Yanling Wang,Xiujiang Li,Xinyu Huang,Ting Huang
BACKGROUND Monascus , a filamentous fungus, produces many bioactive substances. However, in the process of fermentation, Monascus also produces the mycotoxin citrinin. Owing to the presence of citrinin, the safety of Monascus products has been questioned and their wide application limited. Using soybean isoflavones (SI) as exogenous additives, alterations in citrinin production by Monascus aurantiacus Li AS3.4384 (MALA) in different media used for liquid state fermentation were investigated. RESULTS Results showed that the citrinin concentration was 95.98% lower than that of the control group after 16-days fermentation when 20.0 g L −1 SI were added to rice powder and inorganic salt medium. Citrinin production was reduced by 97.24% after 12-days fermentation with 10.0 g L −1 SI in starch inorganic salt medium; 82.52% after 20-days fermentation with 20.0 g L −1 SI in starch peptone medium with high starch content; 45.07% after 14-days fermentation with 5.0 g L −1 SI in starch peptone medium with low starch content; and 82.21% after 14-days fermentation with 20.0 g L −1 SI in yeast extract sucrose medium. CONCLUSION The developed method of removing citrinin is simple, safe, and effective, and it can be applied to reduce the citrinin content of Monascus products. © 2019 Society of Chemical Industry
研究领域:
食品科学
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微生物学
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生物工程
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产品关联
DOI
PubMed
高影响因子
FOOD SCIENCE AND BIOTECHNOLOGY
·
2016
·Vol. 25
·期 4
·1115-1122
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IF 0.699
Feng Yanli,Chen Wanping,Chen Fusheng
Monacolin K (MK) produced by Monascus spp. is mostly used to regulate cholesterol level, while it can be contaminated by citrinin, a mycotoxin yielded by some Monascus strains. To develop production of citrinin-free MK, an M . sp. MS-1 strain, identified as M. pilosus by morphologies and internal transcribed spacer sequences analysis, was isolated from red fermented rice. After 14 days of fermentation, yields of citrinin-free MK could be up to 0.58 mg/mL and 16.45 mg/g in liquid- and solidstate fermentation products under optimal conditions, respectively. Further determination revealed that no citrinin biosynthesis related genes such as ctnA , pksCT , ctnE , and ctnR were detected. Thus, HPLC combined with citrinin-related gene analyses can be used for rapid screening of non-citrinin production Monascus strains.
研究领域:
微生物学
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药学
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生物技术
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DOI
PubMed
高影响因子
BIOLOGICAL CONTROL
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2022
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IF 3.857
Meilin Wei,Solairaj Dhanasekaran,Guillaume Legrand Ngolong Ngea,Esa Abiso Godana,Xiaoyun Zhang,Qiya Yang,Xiangfeng Zheng,Hongyin Zhang
Ochratoxin A (OTA), a widespread mycotoxin contaminant in food and feed, threatens human and animal safety and causes significant economic losses. The aims of the present study were to investigate the elimination properties of Cryptococcus podzolicus Y3 on OTA and its co-degradation efficiency on citrinin (CIT) and OTA. C . podzolicus Y3 eliminated 100% OTA (1 μg/mL) in the PM media within 5 d and in grape juice media within 3 d by intracellular substances. The results of HPLC-FLD and LC-MS showed that OTA was efficiently eliminated into non-toxic ochratoxin α (OTα). The substances responsible for OTA degradation were thermostable and worked effectively at 4 °C and 28 °C, and intracellular proteins were participated in the elimination of OTA. In addition, the results showed that C. podzolicus Y3 degraded OTA and CIT together in NYDB media, although the degradation efficiency was lower than single mycotoxin degradation. Generally, our study highlighted that C. podzolicus Y3 could be a potential strain to eliminate OTA and CIT to secure food products and enhance food safety.
研究领域:
食品科学
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毒理学
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微生物学
✓ 已验证
产品关联
DOI
高影响因子
Toxins
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2019
·Vol. 11
·期 10
·605
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IF 3.895
Erjing Chen,Ying Xu,Biao Ma,Haifeng Cui,Chuanxin Sun,Mingzhou Zhang
A fluorescent immunochromatographic test strip (FICTS) based on the use of europium nanoparticles (EuNPs) was developed and applied to detect citrinin (CIT) inMonascusfermented food. The sensitivity of the immunoassay to detect CIT was greatly improved by the use of a specific monoclonal antibody to attach EuNPs to form a probe. Under optimum conditions, the visual detection limit was 2.5 ng/mL, and the detection limit of the instrument was 0.05 ng/mL. According to the results, the IC50was 0.4 ng/mL. Matrix interference from variousMonascusfermented foods was investigated in food sample detection. The immunosensor also demonstrated high recoveries (86.8–113.0%) and low relative standard deviations (RSDs) (1.8–15.3%) when testing spikedMonascusfermented food. The detection results of this method showed a good correlation (R2> 0.98) with high-performance liquid chromatography (HPLC). The results showed that the FICTS method could be used as a rapid, sensitive method to detect CIT inMonascusfermented food.Keywords:Citrinin;Fluorescent immunochromatographic assay;Europium nanoparticles;Monascusfermented foodKey Contribution:In this study, the developed FICTS method greatly improved the sensitivity of detecting CIT inMonascusfermented food.
研究领域:
食品科学
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分析化学
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毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Ovarian Research
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2024
·Vol. 17
·期 1
·1-16
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IF 3.8
Wang Shiwei,Wu Xuan,Yang Juan,Peng Yuwan,Miao Fulu,Li Min,Zeng Juan
Background Sterigmatocystin (STE) is a mycotoxin widely found in contaminated food and foodstuffs, and excessive long-term exposure to STE is associated with several health issues, including infertility. However, there is little information available regarding the effects of STE toxin on the female reproductive system, particularly concerning oocyte maturation.Methods In the present study, we investigated the toxic effects of STE on mouse oocyte maturation. We also used Western blot, immunofluorescence, and image quantification analyses to assess the impact of STE exposure on the oocyte maturation progression, mitochondrial distribution, oxidative stress, DNA damages, oocyte ferroptosis and asymmetric division defects.Results Our results revealed that STE exposure disrupted mouse oocyte maturation progression. When we examined the cellular changes following 100 µM STE treatment, we found that STE adversely affected polar body extrusion and induced asymmetric division defects in oocytes. RNA-sequencing data showed that STE exposure affects the expression of several pathway-correlated genes during oocyte meiosis in mice, suggesting its toxicity to oocytes. Based on the RNA-seq data, we showed that STE exposure induced oxidative stress and caused DNA damage in oocytes. Besides, ferroptosis and α-tubulin acetylation were also found in STE-exposed oocytes. Moreover, we determined that STE exposure resulted in reduced RAF1 protein expression in mouse oocytes, and inhibition of RAF1 activity also causes defects in asymmetric division of mouse oocytes.Conclusion sCollectively, our research provides novel insights into the molecular mechanisms whereby STE contributes to abnormal meiosis.
研究领域:
生殖毒理学
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细胞生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2024
·
IF 8.5
Shi Tang,Baoshan He,Yao Liu,Longdi Wang,Ying Liang,Jinshui Wang,Huali Jin,Min Wei,Wenjie Ren,Zhiguang Suo,Yiwei Xu
Citrinin (CIT) is a mycotoxin with nephrotoxicity and hepatotoxicity, presenting a significant threat to human health that is often overlooked. Therefore, a dual-signal mode (DPV and SWV) aptasensor for citrinin (CIT) detection was constructed based on tetrahedral DNA nanostructures (TDN) in this study. Furthermore, PtPdCo mesoporous nanozymes exhibit catalase-like catalytic functions, generating significant electrochemical signals through a Fenton-like reaction. Meanwhile their excellent Methylene Blue (MB) loading capability ensures independent dual signal outputs. The RecJf exonuclease-assisted (RecJf Exo-assisted) process can expand the linear detection range, enabling further amplification of the signal. Under optimized conditions, the constructed aptaensor exhibited excellent detection performance with limits of detection (LODs) of 7.67 × 10 −3 ng·mL −1 (DPV mode) and 1.57 × 10 −3 ng·mL −1 (SWV mode). Due to its multiple signal amplification and highly accurate dual-signal mode detection capability, this aptasensor shows promising potential for the in situ detection.
研究领域:
分析化学
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纳米技术
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食品安全
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毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2021
·
IF 10.618
Chen Zong,Fan Jiang,Xiaoyu Wang,Ping Li,Linru Xu,Hua Yang
A novel chemiluminescence (CL) immunosensor array in combination with a dual-signal amplification strategy was developed for rapid and ultrasensitive detection of multiple mycotoxins in herbal medicine. The multi-component immunosensor array was constructed by immobilizing different bovine serum albumin combined mycotoxins on the corresponding sites of the aldehyde-modified glass slide. After competitive immunoreactions, CL triggered by signal tags captured on all the sensing sites can be collected by a charge-coupled device simultaneously for joint detection of several mycotoxins. By assembling a high ratio of horseradish peroxidase (HRP) and IgG on the surface of gold nanoparticles (AuNPs), a biofunctionalized complex named [email protected] was prepared and served as the primary signal tag to amplify the CL signals. Then, by introducing tyramine signal amplification (TSA) technique, a new round of HRP could deposit around the [email protected] , which brought the secondary CL amplification. As a proof of concept , the CL imaging sensor array was applied to the trace detection of citrinin , aflatoxin B 1 and ochratoxins A in red yeast rice samples. Under optimal conditions, it exhibited wide linear ranges over 4 orders of magnitude and much lower limits of detection than previous works. Owing to the excellent sensitivity (50–57-fold signal amplification and detection limits down to sub-pM level), acceptable throughput (20 tests h −1 ), small amounts of reagents (3.5 μL for each test), simple sample pretreatment (no necessary of separation for 3 mycotoxins), high selectivity , acceptable stability and accuracy, the proposed sensing platform showed broad prospects in the joint monitoring of low-abundant mycotoxins and safety evaluation of herbal medicines.
研究领域:
分析化学
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生物技术
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食品科学
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毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）