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Microwave Extraction of New Polysaccharides From Inonotus obliquus, Structural Characterization, and Antioxidant Activity

Hao Xie,Nurmamat Eliyas

To address the low extraction yield and structural degradation issues of conventional methods, this study optimized microwave-assisted extraction (61°C, 64 min, 30:1 mL/g ratio) to obtain Inonotus obliquus polysaccharides (IOP), followed by DEAE-52 and Sephadex G-100 purification to isolate two fractions: neutral IOP-1 (12.67 kDa) and acidic IOP-2 (26.13 kDa). Monosaccharide analysis revealed both as heteropolysaccharides containing mannose, glucose, and galactose with distinct compositional ratios. Structural characterization showed IOP-1 primarily contains 1,4-Glcp, 1,3,6-Glcp, and 1,3-Manp linkages, while IOP-2 features 1,3-Glcp, 1,4-Glcp, 1,4,6-Glcp, and 1,4-Galp linkages. Multiple antioxidant assays demonstrated superior free radical scavenging capacity in both polysaccharides, with IOP-1 exhibiting exceptional activity exceeding typical polysaccharides performance. These findings highlight IOP-1's potential as a novel natural antioxidant for pharmaceutical and food applications. Graphical