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PEST MANAGEMENT SCIENCE · 2022 ·Vol. 79 ·期 1 ·173-182 · IF 4.462

Dynamics of gut microflora across the life cycle of Spodoptera frugiperda and its effects on the feeding and growth of larvae

Dongbiao Lü,Yanlu Dong,Zizheng Yan,Xueying Liu,Yongjun Zhang,Daibin Yang,Kanglai He,Zhenying Wang,Ping Wang,Xiangqun Yuan,Yiping Li

BACKGROUND Spodoptera frugiperda is an important invasive agricultural pest that causes huge economic losses worldwide. Gut microorganisms play a vital role in host feeding, digestion, nutrition, immunity, growth and insecticide resistance. Illumina high-throughput sequencing was used to study the gut microbial community dynamics across the life cycle (egg, 1st to 6th instar larvae, pupae, and male and female adults) of S. frugiperda fed on maize leaves. Furthermore, the gut microbial community and food intake of the 5th instar S. frugiperda larvae were studied after feeding them antibiotics. RESULTS Enterobacteriaceae and Enterococcaceae dominated the gut during growth and feeding of the larvae. The relative abundance of Enterobacteriaceae was higher in the 4th and 6th instar larvae. With the increase in larval feeding, the relative abundance of Enterococcaceae gradually increased. In addition, principal coordinate analysis and linear discriminant effect size analysis confirmed differences in the structure of gut microbiota at different developmental stages. After antibiotic treatment, the relative abundance of Firmicutes, Proteobacteria and Fusobacteriota decreased. The relative abundance of Enterococcus and Klebsiella decreased significantly. Antibiotic treatment inhibited the gut flora of S. frugiperda , which decreased larval food intake and body weight gain, and prolonged the larval stage. CONCLUSION The composition of the gut bacterial community plays an important role in the growth, development, and feeding of S. frugiperda . The results have a certain theoretical value for the development of bio-pesticides targeting intestinal flora. © 2022 Society of Chemical Industry.

研究领域: 昆虫学 · 微生物学 · 农业科学
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FOOD HYDROCOLLOIDS · 2023 · IF 10.7

In vitro fermentation characteristics of oxidized konjac glucomannan and its modulation effects on gut microbiota

Yao Li,Ting Gong,Hongjia Lu,Senquan Ma,Xiong Liu

Depolymerized konjac glucomannan (KGM) had better health benefits than natural KGM. Oxidation and enzyme-hydrolysis were two effective ways to prepare depolymerized KGM. Oxidized KGM (OKGM) and enzyme-hydrolyzed KGM (EKGM) varied in molecular structures, verified by nuclear magnetic resonance and atomic force microscope. The differences in chemical structure definitely influenced the fermentability of depolymerized KGM. Herein, effects of OKGM on in vitro fecal fermentation was investigated, comparing with EKGM at approximately same molecular weights. During fermentation, OKGM groups displayed slightly lower reducing sugar concentrations and higher residual carbohydrate contents than EKGM groups. After fermentation, OKGM groups produced more acetic acids and propionic acids than EKGM groups, while EKGM groups generated more butyric acids than OKGM groups. Meanwhile, relative abundances of the Firmicutes and Lactobacillus in OKGM groups were higher, while relative abundances of the Actinobacteria , Fusobacteria and Bacteroides was higher in EKGM groups. OKGM and EKGM differed in modulating gut microbiota.

研究领域: 食品科学 · 微生物学 · 营养学
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