TALANTA
·
2023
·
IF 6.1
Chihong Ma,Qin Zhou,Jinjin Shi,Hua Gao,Di Huang,Huimin Xue,Han Wang,Zhenzhong Zhang,Sen Yang,Junli Zhang,Kaixiang Zhang
The clustered regularly interspaced short palindromic repeats (CRISPR) system provides a new molecular diagnostic tool for construction of biosensor platforms due to its high programmability and target specificity. Herein, we developed a CRISPR-empowered electrochemical biosensor by combining the advantages of CRISPR/Cas13a and primer exchange reaction (PER), named PER-E-CRISPR, for target amplification-free and sensitive detection of miR-21. Dual-signal amplification procedures involve the binding of target miR-21 induced by CRISPR-based amplification, along with the hybridization of multiple short single-stranded DNA strands with PER concatemers. When target miR-21 is present, CRISPR/Cas13a specifically recognizes the target miRNA , triggering the trans -cleavage activity of CRISPR/Cas13a. Then Cas13a/crRNA/miRNA cleaved the predesigned ribonucleotide site in hairpin 1 (HP1) and released trigger to open hairpin 2 (HP2) modified on the electrode surface. Then PER bridge sequence contained in HP2 is exposed and hybridized with PER concatemers, following multiple short single-stranded DNA tagged with methylene blue (ssDNA-MB) bond with the PER concatemers. Under optimized conditions, PER-E-CRISPR assay for detecting miR-21 exhibits linearity in dynamic range from 10 −13 to 10 −7 M, and we obtained a limit of detection (LOD) of 30.2 fM. The established PER-E-CRISPR biosensor shows perfect practical performance in actual plasma, which may have great promising prospects for miRNA detection in the field of molecular diagnosis.
研究领域:
分子诊断
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生物技术
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电化学
·
纳米技术
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DOI
PubMed
高影响因子
Sensors and Actuators Reports
·
2025
·
IF 7.6
Junting Liang,Dihan Duan,Lubing Sun,Jinming Li,Mengke Wang,Ziyi Chang,Rick F. Thorne,Chuanliang Chen,Demin Duan
Cysteine (Cys) is essential for numerous physiological processes and play a vital role in disease diagnostics, underscoring the necessity for effective and sensitive detection techniques for Cys. In this research, we introduce an ultrasensitive colorimetric approach based on oxidase-like Mn 3 O 4 nanozymes. The nanozymes were synthesized through a straightforward wet chemical method and displayed a flower-like globular morphology, showing remarkable oxidase-like catalytic performance with a low K m value of 0.1896 mM and a high V max of 2.8 × 10⁻⁷ M s⁻¹. By leveraging the outstanding catalytic activities of Mn 3 O 4 nanozymes and optimizing the TMB concentration, we developed a novel and sensitive colorimetric sensor for Cys detection. Experimental results indicated a detection range for Cys between 0.5 and 45 μM, with a lower limit of detection (LOD) of 0.02636 μM. This established method for detecting Cys reveals significant promise for the advancement of effective sensing systems.
研究领域:
生物化学
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分析化学
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纳米技术
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疾病诊断
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Huifang Wu,Yuwei Ling,Shiying Ju,Yubing Chen,Mengqi Xu,Yanfeng Tang
Phosphate (Pi) plays an essential role in aquatic ecosystems as well as in physiological processes. Here, a dual-emission probe for the sensitive, specific and visual analysis of Pi is fabricated by coordinating Eu 3+ with luminol and 2,6-pyridinedicarboxylic acid (DPA). Pi can significantly enhance the characteristic fluorescence of Eu 3+ at 615 nm by promoting energy transfer from DPA to Eu 3+ and reducing the quenching effect of water molecule, luminol with inherent emission at 423 nm further enhances the Eu 3+ fluorescence. Accordingly, ratiometric detection of Pi can be achieved with the fluorescence ratio F 615 /F 423 as a function of Pi concentration. Linearity between F 615 /F 423 and Pi concentration in the range of 0.1–25 μM is shown, and the limit of detection (LOD, 3σ/K) for Pi is 0.027 µM. In addition, a continuous change in the fluorescence color of the probe from blue to red is observed with increasing Pi concentration under a UV lamp, and a smartphone-based visual method is used for the convenient and effective semi-quantitative determination of Pi. The dual-emission probe has been successfully applied to ratiometric and visual analysis of Pi in human urine and environmental water samples, and adequate results are obtained.
研究领域:
分析化学
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环境科学
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生物医学工程
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DOI
PubMed
高影响因子
JOURNAL OF APPLIED ELECTROCHEMISTRY
·
2022
·Vol. 53
·期 3
·559-569
·
IF 2.925
Li Shun,Niu Airong,Lan Cuixia,Xu Xuanming,Sun Shukai,Xuan Chao,Zhao Peng,Tian Qingwu,Zhou Tingting
A novel electrochemical sensor of multi-walled carbon nanotube (MWCNT) and stearyl trimethyl ammonium chloride (STAC) modified acetylene black paste electrode (AB-PE) was successfully established. An AB/MWCNT/STAC-paste electrode was constructed using the AB electrode with MWCNT and STAC as the electrochemical sensor materials. The performance of the prepared sensor was characterized by electrochemical impedance spectroscopy and cyclic voltammetry. Some factors, such as sensor composition, adsorption, and detection conditions, which affect the property of the sensor, were investigated in detail. The results demonstrated that the prepared sensor has satisfactory selectivity, repeatability, and stability for methotrexate (MTX) detection. The linear range of the method for MTX detection was 0.02–8.0 μM with a limit of detection (LOD) of 1.81 nM. Finally, the sensor was successfully used for detecting the MTX concentrations in the samples of serum and saliva. The prepared sensor could be a fresh platform for the swift and accurate MTX detection in clinical samples. Graphical abstract
研究领域:
电化学
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纳米技术
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分析化学
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临床化学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Yuanyuan Liu,Bangyan Liu,Lian Xia,Hongyan Yu,Qiaodie Wang,Yuangen Wu
Although methods based on mass spectrometry or immunoassays can meet the demands of paraquat detection, their application is limited by a lack of convenience or stability. Herein, a facile visual aptamer-based chromatographic strip (CS) was fabricated for the rapid paraquat detection using the cationic polyelectrolyte poly(diallyl dimethyl ammonium chloride) (PDDA) as a powerful capture molecule at test-line (T-line). The PDDA could capture the gold nanoparticles (AuNPs) through strong electrostatic interactions for visual detection. The proposed CS showed an excellent response for paraquat in the range of 20–150 μg·L −1 , and the limit of detection (LOD) was as low as 4.28 μg·L −1 . This detection method has good stability and specific selectivity , and can visually determine paraquat residues more than 30 μg·L −1 by naked eye within 3 min. The results demonstrated that the prepared CS based on aptamer and cationic polyelectrolyte is an accurate and practicable tool for rapid analysis of pesticide residues .
研究领域:
分析化学
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环境科学
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农业科学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Haoyu Wang,Shiting Chen,Jiqing Liu,Wanyue Liu,Wei Chen,Lin Huang,Chao Yan
In recent years, RPA-CRISPR/Cas12a-based electrochemical biosensors have emerged as highly effective tools for nucleic acid detection. However, the random trans -cleavage characteristics of Cas12a on single-stranded nucleic acid sequences can result in the presence of uncertain nucleic acid residues on the electrode surface post-reaction. This situation complicates subsequent sensitization strategies. In this study, we devised a universal sensitization protocol mediated by terminal deoxynucleotidyl transferase (TdT) , a template-independent DNA polymerase and G-rich spherical nucleic acids (G-rich SNAs) with superior performance. Initially, Cas12a was activated by the specific RPA amplification product and cleaved the closed single stranded DNA (C-ssDNA) on the electrode surface. Then, the TdT enzyme extended residual single stranded DNA (R-ssDNA) on the electrode surface, forming G-Quadruplex structures. Subsequently, G-rich SNAs were immobilized on the electrode surface via π-π stacking interactions with the G-quadruplexes. Consequently, a substantial accumulation of hemin occurred within the G-quadruplex cluster structures, generating significant electrochemical signals. We applied this method to the trace detection of Staphylococcus aureus ( S. aureus ) and achieved a good linear calibration curve with concentrations ranging from 0 CFU/mL to 10 4 CFU/mL. Notably, the limit of detection (LOD) was as low as 1.29 CFU/mL, surpassing that of most similar sensors. These results demonstrate that our proposed universal sensitization method effectively addresses the limitations associated with the random trans -cleavage characteristics of Cas12a.
研究领域:
生物传感
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纳米技术
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分子生物学
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电化学
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产品关联
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2021
·
IF 4.556
Kangkang Wang,Meng Wang,Yuli Kou,Changyan Guo,Xin Ma,Bingren Tian,Ying Qi,Wei Wang,Jide Wang
A rapid and sensitive analytical method has been proposed to monitor simultaneously four nitrofuran metabolites (NFMs) in meat products by ultra-high performance liquid chromatography–fluorescence detection (UHPLC-FLD). These NFMs were derivatized by 4-( N,N diphenylamino)benzaldehyde (DABA) by thermostatic ultrasonication within 20 min. The results confirmed that the four NFMs derivatives had excellent fluorescence response at specific excitation (λ ex = 260 nm) and emission (λ em = 500 nm) wavelengths, and all NFMs derivatives could be separated entirely on the reversed-phase Syncronis C18 column within 10 min. The limits of detection (LOD) and quantitation (LOQ) in this new method were 0.21 to 0.28 and 0.69 to 0.92 μg/kg, respectively. The four NFMs displayed excellent linearity (R > 0.998), and the recovery rate was satisfactory, ranging from 91.6% to 104.0%. This method successfully determined the NFMs in fish, shrimp and chicken. The method is highly sensitive and accurate, therefore, it may have potential for detecting four NFMs residues in other foodstuffs.
研究领域:
分析化学
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食品科学
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药物分析
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产品关联
DOI
高影响因子
DYES AND PIGMENTS
·
2023
·
IF 4.5
Yanzhu Liu,Ming Tu,Wei Lang,Qing Guo,Yongxiu Li,Qian-Yong Cao
A new amphiphilic polynorbornene-based copolymer P1 was designed and synthesized successfully by ring-opening metathesis polymerization (ROMP) of polyethylene glycol (PEG) containing monomer NB-PEG and ferreocene-anchored monomer NB-Fc . The copolymer P1 can bind with anionic uranine dye ( UD ) through multiple interactions to form no fluorescent P1 / UD nano-structure ensemble in aqueous solution. The P1 / UD ensemble can be used as indicator displacement assay (IDA) for ATP detection with a high sensitivity and selectivity. Upon the addition of ATP, the fluorescence is significant enhanced for displacement of UD from P1 / UD ensemble. The LOD value of P1 / UD ensemble toward ATP is as low as 35.9 nM. The P1 / UD ensemble exhibits an excellent photo-stability and biocompatibility, and has been successfully used for imaging ATP in living cells.
研究领域:
高分子化学
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分析化学
·
生物医学工程
·
材料科学
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产品关联
DOI
高影响因子
LANGMUIR
·
2024
·
IF 3.7
Jingyao Mao,Yuteng Zhang,Shensong Zhang,Bo Song
The fluorescent probe method has attracted significant research attention due to its high sensitivity and reproducibility in detecting bovine serum albumin (BSA). In this study, we constructed a fluorescent probe for BSA detection by assembling an amphiphilic organic fluorescent molecule, termed 2-(2’-hydroxyphenyl) benzothiazole (HBT-11), with BSA. In an aqueous solution, HBT-11 exhibited a weak fluorescence emission at 501 nm. However, the addition of BSA substantially enhanced the fluorescence emission at 501 nm, indicating that the assembly was driven by electrostatic interactions between HBT-11 and BSA. HBT-11, serving as a fluorescent probe for BSA detection, demonstrated a limit of detection (LOD) as low as 3.92 nmol L–1, excellent photostability, high selectivity, and robust anti-interference capability. Notably, we successfully applied HBT-11 for detecting BSA in fetal bovine serum and selectively imaging BSA in HeLa cells.
研究领域:
分析化学
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生物化学
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分子生物学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 9
·1-10
·
IF 6.408
Wang Yuexin,Chen Rui,Shen Bo,Li Cai,Chen Junman,Wang Yanshuang,Tian Shen,Li Xuemiao,Luo Nini,Liu Rui,Ding Shijia,Zhu Chuanlong,Xia Qianfeng
A multifunctional catalytic nanomaterial (Co-MOF@AuNP@ABEI) composed of cobalt-doped metal–organic frameworks (Co-MOF), gold nanoparticles (AuNP), and N -(4-aminobutyl)- N -(ethylisoluminol) (ABEI) is reported. Co-MOF@AuNP@ABEI exhibits high synergistic and zero-distance catalytic properties, which are beneficial to the improvement of the detection sensitivity of an electrochemiluminescent (ECL) biosensor. After coupling with the ECL system and 3D magnetic walking nanomachine amplification strategy, the Co-MOF@AuNP@ABEI can achieve an ultrasensitive ECL assay of Burkholderia pseudomallei with the limit of detection (LOD) of 60.3 aM, which is 2 and 4 orders of magnitude lower than individual ECL system without the nanomachine (4.97 fM) and individual walking nanomachine (340 fM), and superior to the pathogenic bacteria analyses in the previous report. Moreover, the LOD of the proposed ECL detection system for the determination of B. pseudomallei in serum sample was as low as 9.0 CFU mL −1 . The relative standard deviations (RSD) of ECL intensity for the detection of five B. pseudomallei -spiked serum samples were 4.02%, 0.84%, 0.84%, 1.55%, and 0.21%, respectively. The recoveries of the ECL biosensor for the detection of B. pseudomallei DNA-spiked serum samples were 93.63 ~ 107.83%. Therefore, this work demonstrated that the developed multifunctional catalytic nanomaterial with synergistic and zero-distance catalytic properties can be used as excellent ECL signal reporter to improve the detection sensitivity of ECL biosensor. Graphical abstract
研究领域:
生物传感器
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分析化学
·
纳米技术
·
微生物学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2022
·
IF 12.545
Xiangqin Meng,Sijia Zou,Dandan Li,Jiuyang He,Ling Fang,Haojue Wang,Xiyun Yan,Demin Duan,Lizeng Gao
The coronavirus disease 2019 (COVID-19) pandemic has created a huge demand for sensitive and rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The current gold standard for SARS-CoV-2 detection is reverse transcription-polymerase chain reaction (RT-PCR)-based nucleic acid amplification. However, RT-PCR is time consuming and requires specialists and large instruments that are unattainable for point-of-care testing (POCT). To develop POCT for SARS-CoV-2, we combined recombinase polymerase amplification (RPA) and FeS 2 nanozyme strips to achieve facile nucleic acid amplification and subsequent colorimetric signal enhancement based on the high peroxidase-like activity of the FeS 2 nanozymes. This method showed a nucleic acid limit of detection (LOD) for SARS-CoV-2 of 200 copies/mL, close to that of RT-PCR. The unique catalytic properties of the FeS 2 nanozymes enabled the nanozyme-strip to amplify colorimetric signals via the nontoxic 3,3′,5,5′-tetramethylbenzidine (TMB) substrate. Importantly, the detection of clinical samples of human papilloma virus type 16 (HPV-16) showed 100% agreement with previous RT-PCR results, highlighting the versatility and reliability of this method. Our findings suggest that nanozyme-based nucleic acid detection has great potential in the development of POCT diagnosis for COVID-19 and other viral infections.
研究领域:
分子生物学
·
病毒学
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即时诊断
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Yaqi Yang,Jiejun Li,Zhiwang Luo,Xingnian Li,Jiayi Zou,Chunyan Li,Chao Chen,Yixi Xie,Pengcheng Zhao,Junjie Fei
One-dimensional (1D) ordered mesoporous carbon hybrid nanotubes loaded with bimetallic oxide hollow nanospheres are expected to be very attractive electrocatalysts . In this study, through high-temperature pyrolysis , we generated Mo 2 C@C PNTs with outstanding electrical conductivity , a significant surface area , and effective catalytic activity by wrapping ordered mesoporous polypyrroles on the surface of 1D MoO 3 nanotubes using block polymers that self-assembled. FeMnO 3 hollow porous nanospheres have a large specific surface area, many electroactive sites, and excellent molecular/charge diffusion properties, which can be compounded with 1D ordered mesoporous nitride-rich carbon to solve the problem of poor electrical conductivity of metal oxides and to prevent the accumulation of 1D nanotubes. The composite showed excellent electrocatalytic activity towards rutin due to the synergistic interaction between Mo 2 C@C PNTs and FeMnO 3 . In the best case, rutin has a limit of detection (LOD) as low as 0.35 × 10 −9 M and a wide linear range (3 × 10 −9 to 3.1 × 10 −6 M). The sensor displays a remarkable level of selective response to rutin in the presence of other co-interfering species. Furthermore, it has superb repeatability and reproducibility. The sensor has shown outstanding recovery (95.7–104.1 %) in the practical viability of detecting rutin in beverages. This work not only provides a new candidate for the accurate detection of rutin but also provides insight into the exploration of advanced sensing materials.
研究领域:
材料科学
·
电化学
·
纳米技术
·
分析化学
·
传感器
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）