Food Analytical Methods
·
2021
·Vol. 14
·期 11
·2420-2429
·
IF 3.366
Cao Jinping,Cheng Yuanzhi,Xu Bocheng,Wang Yizhen,Wang Fengqin
To ensure reasonable intake of selenium for humans and provide insights on the development and nutritional analysis of high-quality selenium-enriched food, we established a reliable and highly sensitive method for the determination of selenium species in Z0206 selenium-enriched polysaccharide (Se-EPS). By optimizing the pre-treatment conditions (different enzymes, enzyme combinations, enzymatic time, concentration of enzymes, and centrifugation time), we found that Se-EPS can be effectively hydrolyzed by proteinase K for 18 h, followed by HPLC-ICP-MS determination. The developed method presented high sensitivity with the limit of detection and quantitation in the range of 0.44–2.35 μg L −1 and 1.54–7.21 μg L −1 . The relative standard deviation (RSD) was all less than 10% and recovery was greater than 80% except for SeCys2, which further validated the repeatability and accuracy of the proposed method. Under the optimized conditions, we could determine 4 selenium species: elemental selenium (78.37%), seleno-methionine (14.27%), selenocystine (0.03%), and Se-methylselenocysteine (5.22%) in Se-EPS. This work provides new insights into the determination of different selenium species in selenium-enriched food.
研究领域:
分析化学
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营养科学
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食品科学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Ziyi Wang,Ruimin Song,Weigen Chen,Jianxin Wang,Pinyi Wang,Zifeng Ming,Zhixian Zhang
In order to improve the limit of detection (LOD) of trace substances, a general signal analysis and processing method based on cross-correlation theory is proposed, including correlation detection methods and multi-channel signal modified methods. In this paper, both methods were validated on Raman and GC-MS detection, trace substance in insulating oil (dibenzyl disulfide) is introduced as an example, and verification test of multi-substances detection is also conducted. More generally, the cross-correlation theory based method can be applied to all detection based on spectral peak areas. After applying this method, the LOD can be considerably improved. The traditional detection method based on linear fitting between characteristic peak area and trace substance concentration is implemented as a benchmark. The LOD of Raman detection in traditional way is 338.51 ppm, The LOD of GC-MS detection in traditional way is 317.26 ppb. The correlation detection results show that the LOD of Raman spectroscopy and GC-MS are respectively increased by 1.90/2.76 times compared with traditional way. And when the multi-channel signal modified method is introduced, the qualitative LOD of Raman detection reaches 9.8 ppm (number of channel is 100), the qualitative LOD of GC-MS detection reaches 39.1 ppb (number of channel is 10). Comparative study with conventional noise reduction methods have also been carried out, and the results show that correlation detection and multi-channel signal modified method are superior to them.
研究领域:
分析化学
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信号处理
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光谱学
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DOI
高影响因子
Nanoscale
·
2019
·Vol. 11
·期 30
·14322-14329
·
IF 6.97
Baosheng Du,Chengchun Tang,Dan Zhao,Hong Zhang,Dengfeng Yu,Miao Yu,Krishna C. Balram,Henkjan Gersen,Bin Yang,Wenwu Cao,Changzhi Gu,Flemming Besenbacher,Junjie Li,Ye Sun
Development of fluorescence enhancement (FE) platforms based on ZnO nanorods (NRs) has sparked considerable interest, thanks to their well-demonstrated potential in chemical and biological detection. Among the multiple factors determining the FE performance, high-order waveguide modes are specifically promising in boosting the sensitivity and realizing selective detection. However, quantitative experimental studies on the influence of the NR diameter, substrate, and surrounding medium, on the waveguide-based FE properties remain lacking. In this work, we have designed and fabricated a FE platform based on patterned and well-defined arrays of vertical, hexagonal prism ZnO NRs with six distinct diameters. Both direct experimental evidence and theoretical simulations demonstrate that high-order waveguide modes play a crucial role in FE, and are strongly dependent on the NR diameter, substrate, and surrounding medium. Using the optimized FE platform, a significant limit of detection (LOD) of 10−16 mol L−1 for Rhodamine-6G probe detection is achieved. Especially, a LOD as low as 10−14 g mL−1 is demonstrated for a prototype biomarker of carcinoembryonic antigen, which is improved by one order compared with the best LOD ever reported using fluorescence-based detection. This work provides an efficient path to design waveguiding NRs-based biochips for ultrasensitive and highly-selective biosensing.
研究领域:
纳米技术
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生物光子学
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生物传感器
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分析化学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 4
·1-9
·
IF 5.7
Tu Xinman,Deng Lamei,Ren Boyuan,Xu Zihan,Zhang Li
A conventional colorimetric method based on CuS-catalyzed H 2 O 2 is improved by a dual-modification strategy and employed for thioglycolic acid (TGA) determination. The doping of Co(II) can enhance ion exchange efficiency. Meanwhile, the modification of g-C 3 N 4 can increase specific surface area and decrease unspecific aggregation. The constructed g-C 3 N 4 /Co-CuS nanocomposite exhibited a favorable catalytic feature. A Michaelis constant (K m ) value of 0.02 mM has been achieved, which is 1/160 of those of CuS and horseradish peroxidase (HRP). The g-C 3 N 4 /Co-CuS displays a rapid color response in 3 min and resulted in a stable measurable signal within 10 min. In the determination procedure, the sulfhydryl contained in TGA is capable of preventing TMB oxidation via competing the · OH produced by catalysis and caused a color distinction that is related to the TGA amount. The distinctions of absorbance (λ max = 652 nm) of different concentrations of TGA are recorded. Linearity is obtained in the ranges of 2.5 – 20 µM and 20 – 160 µM, and the LOD is 0.14 µM. In the real sample assays of perm agent and Qianhu lake water, the recoveries were 96.70 – 106.84% and 100.21 – 101.90%, respectively. This demonstrates that the proposed dual-modification strategy for CuS contributes to highly efficient and convenient determination of TGA in daily cosmetics and water analysis. Graphical Abstract
研究领域:
分析化学
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材料科学
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环境科学
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化妆品科学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Tianqi Liu,Xuejuan Wan,Youwei Yao
A Rhodamine B based dual-response sensor was developed for the independent sensitive and selective fluorescence sensing of Pb 2+ and Al 3+ . Initially, TQ1 exhibited one major fluorescence emission peak centered at 461 nm in ethanol/HEPES solution (1/5, pH 7.4), where HEPES was 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid. After the addition of Pb 2+ , an obvious red shift of 55 nm in fluorescence emission can be clearly observed, accompanied by a fluorescence color conversion, from bluish violet to greenish yellow under 365 UV light. As for Al 3+ , the fluorescence emission of TQ1 experienced a much more remarkable red shift of 124 nm, and its fluorescence color also changed to red. Furthermore, the fluorescence sensing process of Pb 2+ and Al 3+ were completely independent and free from interference of each other via changing the buffer concentration. Detection limit of the probe TQ1 towards Pb 2+ and Al 3+ were 8.3590 × 10 −7 M (linear range 1.6–10.0 μM) and 1.0568 × 10 −8 M (linear range 1.0–10.0 μM) respectively. The complexation properties of TQ1 toward Pb 2+ and Al 3+ were studied in detail.
研究领域:
分析化学
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环境毒理学
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生物传感
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分子探针
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重金属检测
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DOI
高影响因子
OPTICAL MATERIALS
·
2021
·
IF 3.08
Meng-Xia Wei,Na Wei,Lan-Fang Pang,Xiao-Feng Guo,Hong Wang
Through facile and straightforward hydrothermal treatment of quercetin, green-emitting carbon dots (g-CDs) were synthesized. The as-prepared g-CDs exhibited good water solubility and stability. Moreover, the strong fluorescence of obtained g-CDs at 535 nm could be quenched by dopamine (DA), which was proven to be effective in detecting DA ranging from 2.0 to 40.0 μM with a low limit of detection (LOD) of 0.52 μM. Additionally, the g-CDs further showed its feasibility for DA determination in human serum with satisfactory recoveries (95.58%–106.87%), indicating that the g-CDs can be used as a promising tool for the detection of DA in human serum.
研究领域:
分析化学
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生物化学
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纳米技术
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Jia Hui He,Yun Ying Cheng,Tong Yang,Hong Yan Zou,Cheng Zhi Huang
Mercury ions (Hg 2+ ) are one of the compulsory items in the quality control of herbal medicines for its serious toxicity to human health. Highly selective and sensitive Hg 2+ detection, especially in complex real samples, is still challenging. In this work, Fluorescent (FL) carbon dots (CDs) with a core-shell structures composed of the crystalline core of stacked sp2-hybridized carbon layers and the shell of functional groups on the periphery of carbon layers are facilely prepared through a one-step hydrothermal synthetic route. They can specifically interact with Hg 2+ in aqueous medium to form aggregates, during which coordination of carboxyl functional groups on the surface of CDs with Hg 2+ occurred, which facilitated electron transfer from the CDs to Hg 2+ . As a result, fluorescence of the CDs was quenched with a high efficiency, making the detection of Hg 2+ highly sensitive with the limit of determination (LOD) of 2.2 nM (3σ). With that, detection of Hg 2+ in the complex compound herbal medicines samples with highly reproducible results has been successfully realized by using the as-prepared CDs, showing that fluorescent CDs-based probe may have great potential in the quality controls of heavy metals for pharmaceutical analysis.
研究领域:
分析化学
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药物分析
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环境科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2020
·
IF 3.721
Lingfei Liu,Qin Hu,Huijuan Sun,Jie Han,Yangni Pan,Zhen-quan Yang
This work reports the development of a new analytical method for the rapid detection of curcumin in food samples by using bluish green emissive fluorescence carbon quantum dots (BG-CQDs) as nanoprobe. The sensing mechanism behind the detection work is the quenching effect of curcumin on the fluorescence intensity of BG-CQDs, where curcumin and BG-CQDs interact via a combined effect of inner filter effect (IFE) and dynamic interaction process. After optimisation of the experimental parameters, the developed analytical method is capable of detecting curcumin with good selectivity and high sensitivity, achieving good linearity in the concentration range of 0.01–25.0 μM. A limit of detection (LOD) of 28.7 nM was obtained, which is lower than that of any of the previously reported CQDs-based fluorescence methods. The developed analytical method was applied to the analyses of food samples with a high degree of accuracy and precision. This work highlights a robust BG-CQDs-based analytical platform of rapid response, low cost, great simplicity, high selectivity and favourable sensitivity, which exhibits immense potential for the accurate monitoring of the curcumin content in dietary food samples.
研究领域:
分析化学
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食品科学
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纳米技术
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产品关联
DOI
高影响因子
ANALYST
·
2020
·Vol. 146
·期 3
·956-963
·
IF 3.978
Jun Ma,Gege Feng,Ying Ying,Yong Shao,Yongxin She,Lufei Zheng,A. M. Abd EI-Aty,Jing Wang
Herein, an indirect SERS sensing assay was developed for the determination of glyphosate (Gly) in tap water. The mechanism of detection was based on relieving the inhibitory effect of L-cysteine (L-cys) on a Au–Pt nanozyme by combining Gly with L-cys through divalent copper ions (Cu2+). In this method, a novel nano-chain-like Au–Ag composite with good repeatability was successfully fabricated to detect SERS signals of oxTMB without disturbing TMB Raman signals. Under optimal conditions, the SERS signal intensity of oxTMB (at 1605 cm−1) was proportional to the concentration of Gly. The results showed a good linear response over the concentration ranges of 10 μg L−1 to 1000 mg L−1. The limit of detection and limit of quantitation of Gly were found to be 5 μg L−1 and 10 μg L−1, respectively. In addition, good anti-interference ability against interfering cations and structural analogues deserves to be mentioned. This SERS assay can be used for detection of Gly in tap water that can meet the needs of practical detection.
研究领域:
分析化学
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环境科学
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Wen Yun,Wanshan Wang,Yiyan Lin,Ruiqi Wang,Xia Ha,Li Yang,Yue Jiang,Xin Zhang
A catalytic hairpin self-assembly (CHA) amplification method was developed for CAP detection based on cross-shaped DNA and UiO-66. MOF was used to quench the fluorescent signal of FAM labeled DNA. Cross-shaped DNA with four fluorophore group (FAM) was utilized to enhance the fluorescent intensity. CAP could open hairpin structure of H-apt and induce CHA reaction. The product of CHA hybridized with cross-shaped DNA, resulting its leaving from the surface of UiO-66 and recovery of fluorescent signal. The limit of detection (LOD) was low to 0.87 pM. This method had been successfully applied for the detection of CAP in actual samples. Importantly, the high sensitivity was attributed to the great amplification efficiency of CHA, strong fluorescent intensity of cross-shaped DNA structure and great fluorescent quenched efficiency of UiO-66.
研究领域:
分析化学
·
纳米技术
·
生物技术
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Shan Xu,Peifang Chen,Xichi Lin,Imran Mahmood Khan,Xiaoyuan Ma,Zhouping Wang
Histamine is a significant biomarker to assess the freshness of fish products. In this study, a novel MOF-based SERS sensor for histamine determination was synthesized by wrapping PVP-capped Au nanoflowers with a ZIF-67 shell (Au [email protected] ). The highly branched Au NFs core exhibited a strong electromagnetic field enhancement effect and provided an ultra-sensitive SERS fingerprint spectrum, while ZIF-67 shell was the contributor to enrich the target and stabilize the substrate. The morphology of the core-shell structures can be easily controlled by the concentrations of the capping agent PVP and MOF precursor Co ion . Consequently, 4-MBA pre-grafted on the optimized SERS substrate can act as the Raman internal standard (IS) to eliminate signal fluctuations through standardizing all spectra against its peak at 1074 cm −1 . Moreover, as the specific receptor for histamine molecules, 4-MBA helped reach the low detection sensitivity, where the SERS intensity ratio, I 1172 /I 1074 presented a good linear relationship towards the histamine concentrations (10 −3 -10 −7 M) with the LOD of 0.87 × 10 −7 M (R 2 = 0.9930). Furthermore, the application in monitoring fish spoilage process demonstrated the feasibility and reliability of the developed sensor. This work provided a facile strategy to construct MOF-based SERS substrate as a potential platform for the shelf-life prediction of fish products.
研究领域:
材料科学
·
分析化学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2023
·Vol. 15
·期 41
·5483-5491
·
IF 3.1
Shengyu Cao,Shanshan Huang,Chudi Yang,Lili Lian,Minhong Ren,Dazhi Sun
Phenoxy carboxylic acid (PCA) herbicides are commonly used herbicides that can easily accumulate in soil, groundwater, crops, and vegetable surfaces. Thus, they pose a serious risk to human health. Accurate detection of trace amounts of PCAs in various matrixes is crucial. Herein, ZIF-67-modified magnetic nanoparticles (MNPs, ZIF-67@Fe3O4) were prepared by growing ZIF-67 on the surface of Fe3O4 MNPs. The introduction of ZIF-67 improved the dispersion of Fe3O4 nanoparticles in water and enhanced their extraction performance for PCAs. When an eluent consisting of ammonia water and acetonitrile (5% : 95%; v/v) was employed, 10 mg of ZIF-67@Fe3O4 displayed optimal extraction performance for PCAs in a 20 mL sample solution at a pH of 3. We achieved a limit of detection ranging from 0.014 μg L−1 to 0.056 μg L−1 for four types of PCA herbicides by using the newly developed method. Notably, the values were considerably lower than the maximum concentration levels of PCAs in drinking water set by the Environmental Protection Agency. The relative recovery rate of PCAs using ZIF-67@Fe3O4 ranged from 83.75% to 117.07% when applied to river water and apple samples. These results demonstrate the great potential of ZIF-67@Fe3O4 in determining the residues of organic pesticides in real samples.
研究领域:
环境科学
·
分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）