PROTEIN AND PEPTIDE LETTERS
·
2018
·Vol. 25
·期 8
·776-782
·
IF 1.039
Huang Xiaoting,Liu Haiyang,Fang Wei,Lin Yan,Tan Ying
Background: Carbohydrate antigen 15-3 (CA15-3), a specific breast cancer-related biomarker in serum, can provide direct information to monitor a patient's postoperative status and can predict recurrence and metastasis of breast cancer. The conventional Enzyme-Linked Immunosorbent Assay
(ELISA) is an effective approach for CA15-3 detection, but there is a high limit of detection which is more than 1 U mL-1. Therefore, a convenient, highly sensitive and specific CA15-3 detection method is needed for earlier prognoses of breast cancer. Objective: In this work, we aim to
develop a novel sensitive and selective immunofluorescence assay for the highly effective detection of CA15-3. Method: We pre-coat the antibody I onto the solid phase to capture antigen CA15-3. The A10254- labeled antibody II identified by electrophoresis and fluorescence spectra recognized
the antigen CA15-3 and formed the sandwich format. The fluorescence signal of A10254 directly relating to the amount of antigen could be detected and quantified by the microplate reader excited at 440nm. Results: The obtained method for CA15-3 detection showed lower limits of detection
(0.11 and 0.18 U mL-1) in a linear range of 0.25-14 U mL-1 in the buffer and 2% serum, respectively. This assay exhibited a good specificity and reproducibility in 2% serum with lower relative standard deviations in different tested concentrations. Conclusion: The developed immunofluorescence
assay enhanced the performance of the conventional CA15-3 ELISA kit. Moreover, the acceptable accuracy and good reproducibility in diluted serum indicated the assay has great potential for the diagnosis of breast cancer in clinical practice.
研究领域:
癌症诊断
·
生物医学工程
·
临床化学
✓ 已验证
DOI
PubMed
高影响因子
ACS Omega
·
2018
·
IF 0
Jinting Gao,Jinjin Yin,Zhanhui Tao,Yaqing Liu,Xiaodong Lin,Jiankang Deng,Shuo Wang
Whether short-term or long-term, overexposure to an abnormal amount of copper ion does significant harm to human health. Considering its nonbiodegradability, it is critical to sensitively detect copper ion. Herein, a novel fluorescent strategy with a “turn-on” signal was developed for highly sensitive and specific detection of copper ion (Cu2+). In the present investigation, we found that Cu2+ exhibits excellent peroxidase-like catalytic activity toward oxidizing the nonfluorescent substrate of Amplex Red into the product of resofurin with outstanding fluorescence emission under the aid of H2O2. Thus, an enzyme-free and label-free sensing system was constructed for copper ion detection with quite simple operation. To ensure the detection sensitivity and reproducibility, the amount of H2O2 and incubation time were optimized. The limit of detection can reach as low as 1.0 nM. In addition, the developed assay demonstrated excellent specificity and could be utilized to detect copper ion in water samples including tap water and bottled purified water without standing recovery.
研究领域:
分析化学
·
环境科学
·
生物医学
✓ 已验证
DOI
Small
·
2024
·
IF 13
Yiming Dong,Niu Feng,Puyue Liu,Qiaoling Wei,Xuewen Peng,Feng Jiang,Yiping Chen
The improper use and overuse of antibiotics have led to significant burdens and detrimental effects on the environment, food supply, and human health. Herein, a magnetic solid-phase extraction program and an optical immunosensor based on bimetallic Ce/Zr-UiO 66 for the detection of antibiotics are developed. A magnetic Fe 3 O 4 @SiO 2 @Ce/Zr-UiO 66 metal-organic framework (MOF) is prepared to extract and enrich chloramphenicol from fish, wastewater, and urine samples, and a horseradish peroxidase (HRP)-Ce/Zr-UiO 66@bovine serum protein-chloramphenicol probe is used for the sensitive detection of chloramphenicol based on the dual-effect catalysis of Ce and HRP. In this manner, the application of Ce/Zr-UiO 66 in integrating sample pretreatment and antibiotic detection is systematically investigated and the associated mechanisms are explored. It is concluded that Ce/Zr-UiO 66 is a versatile dual-track material exhibiting high enrichment efficiency (6.37 mg g −1 ) and high sensitivity (limit of detection of 51.3 pg mL −1 ) for chloramphenicol detection and serving as a multifunctional MOF for safeguarding public health and hygiene.
研究领域:
环境科学
·
分析化学
·
公共健康
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 44
·27541-27546
·
IF 3.361
Qingquan Zhang,Jiajia Li,Yuting Su,Xiaoyan Pan,Hongwei Gai
The relatively low sensitivity is the limitation of ELISA in early screening of diseases. Various signal enhancing methods are developed to increase detection sensitivity, but most of them require professional operation and are not compatible with commercial reagent kits or automatic analysis instruments, limiting their application in clinical testing. Here, we present a contactless and user-friendly ball-lens assisted fluorescence enhancing (BFE) method to improve immunoassay sensitivity. The BFE effect is observed in experiment and demonstrated by the simulation. Based on the BFE effect, the detection sensitivity of the immunoassay is improved about 3.6-fold and the limit of detection is lowered by more than 3-fold using commercial reagents and standard ELISA processes. In addition, the BFE effect causes no damage to the linear dependence, specificity and accuracy of clinical plasma measurement. The established method shows a high compatibility with automatic microfluidic immunoassay systems and other signal enhancing techniques, displaying great potential in multi-technique coupling assays and clinical applications.
研究领域:
生物医学工程
·
分析化学
·
临床检验
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2021
·Vol. 44
·期 18
·3398-3406
·
IF 3.645
Tiantian Pang,Xiaohui Chen,Kai Hu,Yongxia Cui,Wenjie Zhao,Huahui Zeng,Zhenqiang Zhang,Shusheng Zhang
In this study, a new Ti 3 C 2 T x -coated fiber was synthesized and utilized as coatings for solid-phase microextraction of seven polychlorinated biphenyls. The as-produced multilayered Ti 3 C 2 T x MXene was characterized by X-ray diffractometer, thermos-gravimetric analysis, scanning electron microscopy, and energy dispersive spectroscopy. It is noteworthy that the Ti 3 C 2 T x showed some attractive features including unique 2D layered structures, large surface area, good hydrophilicity, and rich active recognition sites, endowing it has a high affinity towards the target polychlorinated biphenyls. Subsequently, the affecting parameters on the extraction efficiency of polychlorinated biphenyls were optimized. Under the optimal conditions, a novel method for the analysis of polychlorinated biphenyls in water samples was proposed. The Ti 3 C 2 T x -coated fiber-based solid-phase microextraction method showed good linearity ( r 2 > 0.9928), high enrichment factors (268–442), low limits of detection (0.06–0.15 ng/L), and satisfactory repeatability (RSDs < 7.5%) for the polychlorinated biphenyls. The excellent method recoveries were in the range of 90.0–98.4, 92.0–98.2, and 92.0–98.0% for river water, lake water, and tap water samples, respectively. These results suggested that the proposed Ti 3 C 2 T x -coated fiber-based method represents a promising alternative for the analysis of polychlorinated biphenyls.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2025
·
IF 6.3
Kai Chen,Bin Xiao,JianZhi Luo,XueWei Yuan,Juan Zhang,Songxue Wang,Rui Zhang,Nan Wang,LiQiang Liu,AiKe Li,Ailiang Chen
Edible oil adulteration is a significant food safety concern in today's food fraud landscape. DNA-based detection methods emerging as the most reliable approach for identifying adulterated products. However, the processed oil matrix contains only trace amounts of highly degraded DNA, which significantly increases the difficulty of DNA extraction and greatly reduces the success rate of PCR amplification. To address these technical challenges, we designed species-specific primers for amplicons of varying lengths and systematically evaluated their performance in real-time PCR using DNA extracted from soybean, peanut, and rapeseed oils, followed by application in adulterated oil detection. All primer sets demonstrated high specificity, sensitivity and, most importantly, equal amplification efficiency. Results indicated that shorter amplicons were consistently detectable across all three vegetable oils, while longer amplicons were more likely to yield false negatives in real-time PCR reactions. Soybean oil showed CT values of 29 (74 bp) versus 38 (170 bp), peanut oil exhibited a CT value of 31 (54 bp) with no amplification for 86 bp, and rapeseed oil displayed a CT value of 34 (66 bp) with no signal for 101 bp. The use of shorter primers successfully facilitated the detection of adulterated oil.These findings suggest that amplicon length being a critical determinant for reliable DNA-based identification and authentication of edible oils. Thus, designing amplicons within the 50–80 bp range can significantly enhance the success rate of DNA-based identification and authentication of edible oils.
研究领域:
食品科学
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分子生物学
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产品关联
DOI
高影响因子
Nanoscale
·
2014
·Vol. 7
·期 7
·3100-3108
·
IF 6.739
Yajing Chen,Zhichao Xiong,Lingyi Zhang,Jiaying Zhao,Quanqing Zhang,Li Peng,Weibing Zhang,Mingliang Ye,Hanfa Zou
Highly selective and efficient capture of glycosylated proteins and peptides from complex biological samples is of profound significance for the discovery of disease biomarkers in biological systems. Recently, hydrophilic interaction liquid chromatography (HILIC)-based functional materials have been extensively utilized for glycopeptide enrichment. However, the low amount of immobilized hydrophilic groups on the affinity material has limited its specificity, detection sensitivity and binding capacity in the capture of glycopeptides. Herein, a novel affinity material was synthesized to improve the binding capacity and detection sensitivity for glycopeptides by coating a poly(2-(methacryloyloxy)ethyl)-dimethyl-(3-sulfopropyl) ammonium hydroxide (PMSA) shell onto Fe3O4@SiO2 nanoparticles, taking advantage of reflux-precipitation polymerization for the first time (denoted as Fe3O4@SiO2@PMSA). The thick polymer shell endows the nanoparticles with excellent hydrophilic property and several functional groups on the polymer chains. The resulting Fe3O4@SiO2@PMSA demonstrated an outstanding ability for glycopeptide enrichment with high selectivity, extremely high detection sensitivity (0.1 fmol), large binding capacity (100 mg g−1), high enrichment recovery (above 73.6%) and rapid magnetic separation. Furthermore, in the analysis of real complicated biological samples, 905 unique N-glycosylation sites from 458 N-glycosylated proteins were reliably identified in three replicate analyses of a 65 μg protein sample extracted from mouse liver, showing the great potential of Fe3O4@SiO2@PMSA in the detection and identification of low-abundance N-linked glycopeptides in biological samples.
研究领域:
生物化学
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2017
·
IF 4.162
Pan Fang,Jian-Zhang Pan,Qun Fang
In this paper, we describe a robust sheath flow-based CE-MS interface with minimal interface dead volume based on an extended pattern. A 20 µm i.d. × 90 µm o.d. fused-silica capillary with a chemically-etched thin-wall tip (30 µm o.d.) was used as the separation capillary as well as electrospray emitter, and a 200 µm i.d. × 375 µm o.d. capillary with a tapered tip (40 µm o.d.) was used as the sheath flow capillary. An extendable sheath-flow interface mode was adopted by decreasing the thickness of separation capillary tip and extending the separation capillary tip out from the sheath flow capillary tip, and allowing the sheath flow to be transferred to the separation capillary tip along its outer surface, forming a surface sheath flow to mix with sample flow at the separation capillary tip. Such a strategy could significantly reduce the interface dead volume and thus improve the CE separation efficiency and detection sensitivity, as well as evidently enhance the working reliability of the CE-MS interface. We investigated various factors affecting the interface performance, including capillary extending distance, emitter diameters, sheath flow capillary shape , and sheath flow rate . Under the optimized conditions, a minimal interface dead volume of ca. 4 pL was obtained which is the smallest one compared with previously-reported sheath flow-based CE-MS interfaces. The feasibility and applicability of the present CE-MS interface were demonstrated in the separation of a peptide mixture with high separation efficiency of 2.07–3.38 µm plate heights and good repeatabilities (< 6.1% RSD, n = 5). We except such a simple and robust interface could provide a possible solution for the development of commercial CE-MS interfaces differing from the currently-used ones, and has the potentials to be applied in routine analytical laboratories for various studies such as proteomics, metabolomics, or single cell analysis.
研究领域:
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Luqman Ali,Jie Wei,Fan-Yi Meng,Muhammad Waqas Qureshi,Kishor Kumar Adhikari,Ming-Yu Li,Jun-Ge Liang,Xiao-Long Wang,Xu-Min Ding,Nam-Young Kim,Cong Wang
This work presents the noncontact immobilization of Ti 3 C 2 T x MXene on a planar microwave resonator sensor via a polyimide film to enhance the gas detection sensitivity, enabling the characterization of the part per million-level of acetone gas. Sandwiching a polyimide (PI) film confined an intense electromagnetic field between the resonator’s interdigital capacitor structure and the MXene layer, maintaining a significant interaction between the acetone gas molecules and the resonator’s EM field via the MXene layer. Therefore, contactless immobilization of the MXene layer enhanced the acetone gas detection sensitivity by 110% compared to the resonator gas sensor with MXene deposited directly on the resonator surface. As a proof of concept , two sensor prototypes were implemented using resonators with layers of PI film and MXene and resonators with MXene only and were applied to detect a wide range of acetone gas concentrations. Immobilizing the layers of PI film and MXene on the microwave resonator achieved a linear correlation between the resonant frequency and acetone gas concentration with high sensitivity (17.85 kHz/ppm, a fast response time (60 s), and recovery time (85 s).
研究领域:
材料科学
·
传感技术
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Food Analytical Methods
·
2025
·
IF 3
Na Guanqiong,Chen Ping,Qian Yanya,Meng Danqun,Liu Zhipeng,Shan Yujuan
Traditional fluorescent immunosensors usually suffer from high background signals in food samples, which significantly reduces their detection sensitivity. In this study, δ-iron oxyhydroxide (δ-FeOOH) nanosheets with anti-bacitracin zinc (BAC) monoclonal antibodies (mAb) were conjugated as fluorescence receptors, while amino-functionalized carbon quantum dots (CQDs) as fluorescence donors for BAC. A novel fluorescence immunoassay was developed that eliminated background noise based on the “turn on” principle. Upon introduction of BAC into the sample, the nano-assembled probe showed an increase in fluorescence recovery, leading to higher fluorescence intensity at elevated BAC concentrations. This strategy exhibited a linear response for BAC concentrations ranging from 5 to 40 ng/mL, with a limit of detection (LOD) of 2.04 ng/mL. Our findings present a promising immunological approach for the sensitive and selective detection of BAC residues in food.
研究领域:
分析化学
·
食品科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Xiaoting Song,Changzheng Shi,Huan Chen,Fanqiang Meng,Zhaoxin Lu,Xiaomei Bie
Detection of foodborne pathogens is of great significance for ensuring food safety and human health. Therefore, we developed a new detection method, which combined strand exchange isothermal amplification (SEA) with lateral flow assay strip (LFS), for the rapid and simple detection of Bacillus cereus in food. SEA technique is an isothermal nucleic acid amplification method based on the respiration of DNA denatured bubble to achieve a dynamic dissociation state of DNA double strand. The reaction requires only a pair of primers and DNA polymerase. The primers involved in the reaction were modified with biotin and 6-carboxyfluorescein (6-FAM) respectively, which could combine the reaction system with lateral flow assay strip to achieve the result of visualization of reaction results. In this reaction system, the detection sensitivity of B . cereus pure bacterial solution could reach 7.5×10 2 CFU/mL, and the specificity of the reaction system was good. Without enrichment treatment, the detection sensitivity of artificially contaminated rice cake samples and milk samples could reach 8.3×10 3 CFU/mL and 8.3×10 2 CFU/mL, respectively. The results of test strip and amplification were identical, which proved that the system was suitable for the detection of real samples. In addition, the established detection system has great potential as a preliminary detection tool in resource-limited areas.
研究领域:
微生物学
·
分子生物学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Jiaxin Lu,Shuzhen Dou,Chunning Chen,Yalei Wang,Jingtong Zhai,Hongkun Zhao,Nan Lu
Surface-Assisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) is a prominent tool for detecting small molecules; however, enhancing its detection sensitivity remains a significant challenge. Two strategies are commonly employed to enhance detection sensitivity: enriching analytes through substrate modification and improving the laser desorption/ionization efficiency of the substrate. In this study, we developed a patterned composite hierarchical structure as a SALDI-MS substrate to improve detection sensitivity. The substrate consists of Si nanopillars and Au nanoparticles, and is patterned with hydrophilic spots and hydrophobic surrounding area. The signal intensity of tetrabutylammonium iodide and sulfacetamide is enhanced by 100-fold and 60-fold, respectively, compared to testing on the Si nanopillars array. The sensitivity enhancement on this substrate is primarily attributed to two factors: first, the analytes are enriched on the hydrophilic spots; and second, the laser desorption/ionization efficiency is improved by the introduction of Schottky barriers through the deposition of Au nanoparticles on Si nanopillars, which extends the lifetime of electron-holes. This design offers high sensitivity, with the lowest detection concentrations for dyes, amino acids and sulfonamides reaching the attomole level. Patterned modifications overcome common issues with super-hydrophobic substrate, such as difficult analyte addition and droplet slippage. The pattern also ensures excellent detection reproducibility, with relative standard deviations (RSD) of 2.4 % across different areas of the same substrate and 6.36 % across different substrates. The substrate is suitable for detecting trace levels of dyes and sulfonamides in river water and seafood extract, demonstrating its potential for analyzing real samples.
研究领域:
分析化学
·
质谱技术
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）