SENSORS AND ACTUATORS B-CHEMICAL
·
2019
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IF 6.393
Peng Yu,Yuanming Zhai,Rui-Ying Bao,Zheng-Ying Liu,Ming-Bo Yang,Wei Yang
In chemical industry, elemental sulfur (S) is widely used and always causes environmental and pollution concerns. Hence, the rapid and simple detection of S has become an important issue. In this work, we report an effective approach for visible determination of S. The color of the detection reagent, which was readily prepared by mixing N, N-Dimethylformamide and hydrazine hydrate in a certain ratio, changes from colorless to blue and gradually fades after getting in contact with trace S. The detection reagent can be used for repeated S detection because of the color fading behavior. By measuring the characteristic absorption intensity during detection or the fading time of the mixture, the content of S in the sample can be readily determined and the detection limit can be as low as 0.32 mg/L. An unstable chromogenic organic clathrate generated during detection was found to be responsible for the chromogenic reaction, as revealed by UV–vis, FTIR, NMR, high resolution mass spectrometry measurements and simulation. Thus, this work presents a simple, fast and device-independent approach that can realize semi-quantitative detection of trace S in water, alcohol, food and environmental samples.
研究领域:
化学工业
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环境科学
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分析化学
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产品关联
DOI
高影响因子
TALANTA
·
2023
·
IF 6.1
Tao Peng,Lianhua Dong,Xiaoli Feng,Yi Yang,Xia Wang,Chunyan Niu,Zhanwei Liang,Wang Qu,Qingcui Zou,Xinhua Dai,Minghua Li,Xiang Fang
More than forty antigen testing kits have been approved to response the prevalence of SARS-CoV-2 and its variant strains. However, the approved antigen testing kits are not capable of quantitative detection. Here, we successfully developed a lateral flow immunoassay based on colloidal gold nanoparticles (CGNP-based LFIA) for nucleocapsid (N) protein of SARS-CoV-2 quantitative detection. Delta strain (NMDC60042793) of SARS-CoV-2 have been cultured and analyzed by our developed digital PCR and LFIA methods to explore the relationship between N protein amount and N gene level. It indicated that the linear relationship (y = 47 ×) between N protein molecule number and N gene copy number exhibited very well (R 2 = 0.995), the virus titers and N protein amount can be roughly estimated according to nucleic acid testing. Additionally, detection limits (LODs) of nine approved antigen testing kits also have been evaluated according to the Guidelines for the registration review of 2019-nCoV antigen testing reagents. Only three antigen testing kits had LODs as stated in the instructions, the LODs of Kits have been converted into the N gene and N protein levels, according to the established relationships among virus titer vers. N gene and antigen. Results demonstrated that the sensitivity of nucleic acid testing is at least 1835 times higher than that of antigen testing. We expect that the relationship investigation and testing kits evaluation have the important directive significance to precise epidemic prevention.
研究领域:
病毒学
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分子生物学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
Micromachines
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2024
·Vol. 15
·期 3
·416
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IF 3
Hongzhuang Guo,Xiuyan Jin,Hao Zhang,Ping Gong,Xin Wang,Tingting Sun
In addressing the detection of drug resistance inHelicobacter pylori, we have successfully developed an efficient and highly accurate detection methodology. Initially, we designed and fabricated a microarray chip, which underwent finite element analysis for its optical and thermal characteristics. Ultimately, COC material was chosen as the processing material for the chip, ensuring superior performance. Subsequently, we established a comprehensive detection system and validated its performance. Following that, comparative experiments were conducted for detecting drug resistance inH. pylori. The experimental results indicate that our established methodology aligns with the results obtained using the E-test detection kit, achieving a concordance rate of 100%. In comparison to the E-test detection kit, our methodology reduces the detection time to 1.5 h and provides a more extensive coverage of detection sites.
研究领域:
生物技术
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医学诊断
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微生物学
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材料科学
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2023
·
IF 13.6
Shuai Liu,Hao Qu,Yu Mao,Lili Yao,Baolei Dong,Lei Zheng
Propyl gallate (PG) is a commonly used synthetic phenolic antioxidant in foodstuffs and industrial products. Due to the potential health risk of PG, rapid and on-site detection in food and environment samples are important to guarantee human health. Herein, we demonstrated rapid monitoring of PG by a fluorescence turn-on strategy based on a specific fluorogenic reaction between PG and polyethyleneimine (PEI). Specifically, Ce 4+ with oxidase-mimicking activity oxidized PG to its oxides, which then reacted with PEI through the Michael addition to generate the fluorescent compound. The proposed fluorogenic reaction had good specificity for PG, which could distinguish PG from other phenolic antioxidants and interferences. Furthermore, portable and low-cost organogel test kits were prepared using poly(ethylene glycol) diacrylate for quantitative and on-site detection of PG via a smartphone-based sensing platform. The organogel-based assay detection limit was 1.0 μg mL –1 with recoveries ranging from 80.2% to 106.2% in edible oils and surface water. Suitability of the developed assay was also validated by high-performance liquid chromatography . Our study provides an effective fluorescent approach to rapid, specific, and convenient monitoring of PG, which is useful for diminishing the risk of PG exposure.
研究领域:
分析化学
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环境科学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）