SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Zheng Dou,Wenjia Zhang,Qian Du,Guohua Liu
Whether the copper ion (Cu 2+ ) exposed to us functions as a necessary part of metabolism or toxicant for organs depends on subtle concentration difference of trace-level. Although miniature sensors have combined various recognition probes and signal transducer moieties to form novel sensing scheme, haunting limits on practicality or analytical specifications like sensitivity and selectivity remain thwarting high Cu 2+ detection performance with excellent cost-effectiveness. In this work, we report a Cu 2+ -specific biosensor integrating Deoxyribozyme (DNAzyme) and single-particle plasmon. The basic principle here is that severity of cleavage corresponding to various Cu 2+ concentration determines these elements’ aggregation capacity. The evaluation criteria is set up on the shift of dark-field Localized Surface Plasmon Resonance (LSPR) wavelengths caused by the binding with free AuNPs. Not only excellent analytical specifications like limit of detection (LOD) of 0.082 nM, broad dynamic range from 0.1 nM–5 μM, ultra-specificity are demonstrated but recovery rate with real sample and term of validity is also investigated to be 101.25 ± 9.15 % at 3 nM and longer than 9 days corroborating its potential for practical scenario. Therefore, the sensor is introduced as an attract alternative to remove hindrances for high sensing performance and to generalize on-chip cleavage-inducing spectral shift to heavy metal ions concentration measurement.
研究领域:
分析化学
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生物化学
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纳米技术
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2023
·
IF 6.1
Jiandong Liu,Bin Liu,Juan Liu,Xing-Dao He,Jinhui Yuan,Zabih Ghassemlooy,Hamdi Torun,Yong-Qing Fu,Xuewu Dai,Wai Pang Ng,Richard Binns,Qiang Wu
A critical challenge to realize ultra-high sensitivity with optical fiber interferometers for label free biosensing is to achieve high quality factors (Q-factor) in liquid. In this work a high Q-factor of 10 5 , which significantly improves the detection resolution is described based on a structure of single mode -core-only -single mode fiber (SCS) with its multimode (or Mach-Zehnder) interference effect as a filter that is integrated into an erbium-doped fiber laser (EDFL) system for excitation. In the case study, the section of core-only fiber is functionalized with porcine immunoglobulin G (IgG) antibodies, which could selectively bind to bacterial pathogen of Staphylococcus aureus ( S. aureus ). The developed microfiber-based biosensing platform called SCS-based EDFL biosensors can effectively detect concentrations of S. aureus from 10 to 10 5 CFU/mL, with a responsivity of 0.426 nm wavelength shift in the measured spectrum for S. aureus concentration of 10 CFU/mL. The limit of detection (LoD) is estimated as 7.3 CFU/mL based on the measurement of S. aureus with minimum concentration of 10 CFU/mL. In addition, when a lower concentration of 1 CFU/mL is applied to the biosensor, a wavelength shift of 0.12 nm is observed in 10% of samples (1/10), indicating actual LoD of 1 CFU/mL for the proposed biosensor. Attributed to its good sensitivity, stability, reproducibility and specificity, the proposed EDFL based biosensing platform has great potentials for diagnostics.
研究领域:
光学
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生物传感
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光子学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Yansheng Liu,Zhenle Qin,Xiaobo Jia,Jin Zhou,Hongli Li,Xiaohong Wang,Guofu Wang
Plastics as very important materials have been extensively utilized, and they make people’s lives comparative and economic. However, recent studies find that the micro/nano-plastic particles degenerating from plastic materials especially for food containers may lead to some unknown diseases when they enter into the human lung or stomach. The methods of detecting micro/nano-plastic particles are urgently required, and conventional methods necessitate intricate pre-processing steps and specialized equipment. Hence, this paper introduces a highly sensitive and direct detection method based on Surface-Enhanced Raman Spectroscopy (SERS) in quantitatively and qualitatively analyzing polystyrene (PS) nanoplastics that are drop cast on substrate. The mechanism of SERS performance of the proposed Ag core embedded Au film (Ag@AuFilm) SERS substrate is studied by using the finite-different time-domain (FDTD) method. By optimizing the gap distance between the Ag core and the Au film, PS nanoplastics with three diameters were used to validate the effectiveness of the SERS substrate. The limits of detection (LOD) for PS nanoparticles with diameters of 50 nm, 70 nm, and 310 nm in DI water and real-river water were the same and the corresponding LODs were 50, 50, and 25 μg/mL, respectively. Thus, this study proposes an easily fabricated SERS substrate and provides a rapid, quantitative, and qualitative method for detecting PS nanoplastics. This new way of addressing the pervasive issue of nanoplastics pollution provided an accurate, efficient, and rapid detection of the nanoplastics.
研究领域:
环境科学
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分析化学
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 21
·16036-16041
·
IF 3.84
Jingting Hu,Pengjuan Ni,Haichao Dai,Yujing Sun,Yilin Wang,Shu Jiang,Zhuang Li
AuNPs possess peroxidase-like activity that could catalyze 3,3,5,5-tetramethylbenzidine (TMB) in the presence of H2O2, leading to color change of the solution. Herein we propose a simple and sensitive colorimetric aptasensor for the quantitative analysis of ricin by using AuNPs. It is shown that the peroxidase-like activity of AuNPs can be improved by surface activation with target-specific aptamers. However, with target molecules, the aptamer is desorbed from the AuNPs' surface, resulting in a decrease of the catalytic abilities of AuNPs. The color change of the solution was relevant to the target concentration, and this can be judged by the naked eye and monitored by UV-vis spectrometry. The linear range for the current analytical system was from 0.05 nM to 10 nM. The corresponding limit of detection (LOD) was 0.05 nM. Some other proteins such as thrombin (Th), glucose oxidase (GOx), and bovine albumin (BSA) all had a negligible effect on the determination of ricin. What is more, several practical samples spiked with ricin were analyzed using the proposed method with excellent recoveries. This colorimetric aptasensor is superior to the other conventional methods owing to its simplicity, low cost, and high sensitivity.
研究领域:
分析化学
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生物传感器
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毒理学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Bolong Fang,Xin Liu,Juan Peng,Yuzhi Li,Zhiyong Gong,Weihua Lai
In this study, a type of luminescent porous coordination network-224 (PCN-224) in alkaline conditions was synthesized with the dramatic fluorescence enhancement by 20.4 times, which was explained by the fact that the decrease of Zr 4+ content in alkaline conditions resulted in the partial recovery of the electron cloud density of 4,4′,4′′,4′′′-(Porphine-5,10,15,20-tetrayl) tetrakis(benzoic acid) (TCPP). Given the large overlap between the excitation spectrum of PCN-224 and the absorption band of Ag nanoparticles (Ag NPs), the coating of the Ag layer on PCN-224 triggered the fluorescence quenching effect, which was applied to “turn off” fluorescence immunoassay for sensitive detection of Escherichia coli O157:H7 ( E. coli O157:H7) in milk. The proposed immunoassay reached a low limit of detection (LOD) of 3.3 × 10 2 CFU mL −1 , 29.7 times more sensitive than the conventional ELISA. It will provide a novel alternative strategy for sensitively detecting pathogenic bacteria in the field of food safety.
研究领域:
材料科学
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分析化学
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食品安全
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生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
·
2024
·Vol. 14
·期 3
·135
·
IF 4.9
Hui Chen,Zhiyuan Zhuang,Naihan Xu,Ying Feng,Kaixin Fang,Chunyan Tan,Ying Tan
The ongoing COVID-19 pandemic, driven by persistent SARS-CoV-2 transmission, threatens human health worldwide, underscoring the urgent need for an efficient, low-cost, rapid SARS-CoV-2 detection method. Herein, we developed a point-of-care SARS-CoV-2 detection method incorporating recombinase polymerase amplification (RPA) and DNA–protein crosslinking chemiluminescence (DPCL) (RPADPCL). RPADPCL involves the crosslinking of biotinylated double-stranded RPA DNA products with horseradish peroxidase (HRP)-labeled streptavidin (SA-HRP). Modified products are captured using SA-labeled magnetic beads, and then analyzed using a chemiluminescence detector and smartphone after the addition of a chemiluminescent substrate. Under optimal conditions, the RPADPCL limit of detection (LOD) was observed to be 6 copies (within the linear detection range of 1–300 copies) for a plasmid containing the SARS-CoV-2 N gene and 15 copies (within the linear range of 10–500 copies) for in vitro transcribed (IVT) SARS-CoV-2 RNA. The proposed method is convenient, specific, visually intuitive, easy to use, and does not require external excitation. The effective RPADPCL detection of SARS-CoV-2 in complex matrix systems was verified by testing simulated clinical samples containing 10% human saliva or a virus transfer medium (VTM) spiked with a plasmid containing a SARS-CoV-2 N gene sequence or SARS-CoV-2 IVT RNA. Consequently, this method has great potential for detecting targets in clinical samples.
研究领域:
分子诊断
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病毒学
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生物医学工程
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2019
·
IF 2.398
Zijian Chen,Kai Zhou,Wuzu Ha,Peihong Chen,Huijun Fu,Yudong Shen,Yuanming Sun,Zhenlin Xu
Melatonin (MT) is a hormone secreted by the pineal gland which regulates sleep rhythm and is widely used in health foods. However, MT is sometimes added illegally and excessively in certain health foods, which might cause undesirable side-effects. In this work, an easy-to-use immunochromatographic assay (ICA) based on mouse monoclonal antibody (mAb) for rapid detection of MT was developed for the purpose of safety. Under the optimal conditions, the developed ICA exhibited a visual limit of detection (VLOD) of 10,000 ng/mL and a LOD of 50 ng/mL (strip reader) with the linear range from 50 ng/mL to 5000 ng/mL. Good correlation was obtained between the results of ICA and standard liquid chromatography (LC) in matrix test and blind samples test. The developed ICA can be applied for rapid detection of samples within 30 min, and is potentially an excellent tool for screening of MT in health foods.
研究领域:
分析化学
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食品科学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Di Shao,Shuyun Bi,Rui Zhao,Xiaoyue Sun,Xu Li,Jingfu Yu
Two sensitive surface-enhanced Raman spectroscopy (SERS) analytical methods for dinitolmide and toltrazuril were developed by silver nanoparticles (AgNPs). The dinitolmide and toltrazuril were determined in 0.0565 mol L −1 and 0.0847 mol L −1 NaOH solutions respectively. The enhancement factors (EF) were 1.76 × 10 5 for dinitolmide and 1.27 × 10 5 for toltrazuril. Under optimum conditions, the methods had good linear relationship in the concentration ranges of 0.30–33.30 nmol L −1 for dinitolmide ( I SERS = 1522.52 c + 27,890.46, c :nmol L −1 , R 2 = 0.998) and 0.30–66.70 nmol L −1 for toltrazuril ( I SERS = 1334.29 c + 46,347.83, c :nmol L −1 , R 2 = 0.999). The limits of detection (LOD) (S/N = 3) were 0.915 nmol L −1 for dinitolmide and 1.03 nmol L −1 for toltrazuril. The effects of interferences on the determinations of dinitolmide and toltrazuril were studied. These SERS methods were successfully used to measure the content of dinitolmide and toltrazuril in the duck and chicken samples. The relative standard deviation (RSD) for the detection of dinitolmide and toltrazuril samples could reach 1.45 % ( n = 5). The recoveries were 95.67%–105.39% ( n = 5) and 94.79%–99.44% ( n = 5) for dinitolmide and toltrazuril system respectively.
研究领域:
分析化学
·
兽医学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2022
·
IF 6.556
Xin Wang,Min Zhang,Xiaoxu Pang,Kunlun Huang,Zhiyi Yao,Xiaohong Mei,Nan Cheng
Staphylococcus aureus is a frequent pathogenic bacterium that has a significant detrimental influence on the health of the human body. Therefore, developing a practical and portable detection platform is critical to ensuring food safety. Nanozymes are a kind of engineered nanomaterials with superior enzyme-like activities, providing infinite possibilities for the development of highly sensitive analytical assays. In this study, mesoporous core−shell [email protected] ( [email protected] ) nanozymes were synthesized and then applied as a signal amplifier in Staphylococcus aureus colorimetric immunoassay . At the same time, a careful comparative study of the catalytic performance with natural horseradish peroxidase (HRP), [email protected] nanozymes and their complexes [email protected] (HRP coupling with [email protected] nanozymes) were firstly performed, as well as clever using a colorimeter to achieve portable signal output. [email protected] bioprobes enable remarkable peroxidase-like catalytic activity, resulting in the highest sensitivity with a limit of detection (LOD) improved from 1 × 10 5 to 1 × 10 3 CFU/mL. In all, this work conducted a valuable comparative study and presented an improved strategy for the portable detection of pathogenic bacteria, which was expected to be used in a wide range of applications in food safety regulation and biomedicals arenas.
研究领域:
分析化学
·
生物技术
·
食品安全
·
生物传感器
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Xiaowei Chen,Yujun Feng,Yuanyuan Wang,Xin Yang,Xingping Zhang,Hualin Yang,Yu Zhou
With the gradual increase of oxytetracycline (OTC) residue in the environment, it is particularly important to establish a sensitive and efficient method to detect OTC. In this work, a novel colorimetric and fluorescence dual-mode detection method based on hemin/G-quadruplex (hemin/G4) DNAzyme and zeolitic imidazolate framework-8 (ZIF-8) is developed. OTC can compete with G4 to bind hemin to reduce the amount of hemin/G4. Thus, the oxidation of 3, 3′, 5, 5′-tetramethylbenzidine (TMB) to blue color by hemin/G4 is inhibited. In addition, the OTC or OTC-hemin can be aggregated with the help of ZIF-8 to produce strong green fluorescence due to the aggregation-induced emission (AIE) effect. In colorimetric and fluorescence mode, the linear ranges of the new method are 0.375–18 μM and 0–52.5 μM, with a limit of detection (LOD) of 0.116 μM and 0.040 μM, respectively. Besides, this new method has a good selectivity and recovery rate in the detection of real samples. Therefore, the method can be used for the efficient and sensitive detection of OTC.
研究领域:
分析化学
·
环境科学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Beibei Lu,Jialei Bai,Jingyang Zhang,Huijing Shen,Minglin Wang,Yujing Lian,Zhixian Gao,Yuan Peng
Colorimetric sensing materials have a rising demand in detection applications, especially in the field of naked-eye detection. Herein, a novel iridescent polymeric film that can respond to different Staphylococcus aureus enterotoxin B (SEB) concentrations in the visible range is reported. This film is fabricated by polymerization of methacrylic acid (MAA) and ethylene dimethacrylate (EGDMA) owing an inverse opal array (IOA) structure, and gold nanoparticles (AuNPs) are enchased on the polymer skeleton. This composite structure facilitates the film to respond to SEB at a LOD of 4.29 fg mL −1 , making it the best performing colorimetric material to date. Cryo-Scanning Electron Microscopy and 3D AFM were used to observe the morphology change of the iridescent polymeric film after SEB binding. This film possesses great advantages for ultrasensitive visual detection of real samples. In addition, the iridescent polymeric film has the potential to become a universal sensing material, realizing the ultrasensitive visual detection of hazardous substances without equipment in the field by replacing the recognition element.
研究领域:
材料科学
·
分析化学
·
生物传感
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Hong Chen,Junyan Liu,Mengna Wan,Shangyi Zhao,Xiaolong Li,Chuan Liu,Xuanping Zhao,Junjie Li,Qingzhen Ma,Cuixiang Li,Xueqin Wang
Fruit juice is kind of popular beverage food worldwidely, and its total antioxidant capacity (TAC) is generally considered as an important index for people’s rational diet, thus accurate evaluation of its TAC is essential for human health guidance. Herein, we constructed a sensitive Fe 3 O 4 @MnO 2 nanocatalyst that was delicately cored with magnetite nanoparticles (Fe 3 O 4 NPs) and encapsulated with manganese dioxide (MnO 2 ) nanoshells for fast and accurate TAC colorimetric analysis in fruit juice. The data showed the fabricated Fe 3 O 4 @MnO 2 nanocatalysts performed with remarkable oxidase mimetic activities and could catalyze oxidization of colorimetric substrate without additional H 2 O 2 , and the limit of detection (LOD) was about 0.39 μM for ascorbic acid (AA) evaluation. Applied in commercial fruit juice products, the fabricated Fe 3 O 4 @MnO 2 nanocatalysts showed a reliable and visual assessment for the accurate TAC analysis. In addition, the fabricated Fe 3 O 4 @MnO 2 nanocatalysts could be easily recycled due to their intrinsic superparamagnetic property. Together, an intellegent Fe 3 O 4 @MnO 2 nanocatalyst was well developed for visual TAC analysis in fruit juice with convenient applications in future.
研究领域:
纳米技术
·
分析化学
·
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）