ANALYTICAL SCIENCES
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2021
·Vol. 37
·期 2
·315-320
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IF 2.081
Siqingaowa HAN,Chen ZHANG,Zhijie CHEN,Xuanyu SHA,Wuliji HASI
This paper describes a method based on surface enhanced Raman spectroscopy (SERS) technology for rapid detection of dezocine in urine and serum. Firstly, an Ag colloid substrate was prepared and characterized. Then the Raman characteristic peaks of dezocine were assigned from both theoretical and experimental aspects. Finally, the Raman peak at 661 cm−1 was selected as its characteristic peak to perform SERS detection on dezocine in urine and serum, and the detection limits of dezocine in urine and serum were determined. The relationships between the characteristic peak intensity and the concentration of dezocine in urine and serum were fitted and the recovery rates were calculated. This rapid, accurate and non-destructive method establishes a good foundation for rapid on-site detection of dezocine in biological samples.
研究领域:
分析化学
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生物医学工程
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药物分析
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DOI
PubMed
高影响因子
Analytical Methods
·
2014
·Vol. 7
·期 3
·1036-1045
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IF 1.938
Xiaozhi Wang,Weijun Zhao,Lingfeng Li,Xiangyang Wang,Peng Li,Yu Wang,Jikui Luo
Sulfites (sulfur dioxide) and inorganic sulfites are types of food additives and preservatives, widely used in the production of food and herbal medicines (HMs). However, the overingestion of sulfites and its associates are harmful to human health and may cause medical complications. Various methods and instruments have been developed for measuring sulfites in foods and HMs, but they typically suffer from many issues, such as high detection limitation, inaccurate and non-reliable results, time and labor-intensive sample preparation, and high cost. Herein, we present a fast, sensitive, and quantitative method to determine sulfites in HMs using field asymmetric-wave ion mobility spectrometry (FAIMS) coupled with a headspace bubbling method. The headspace air bubbling method is effective and efficient at generating stable sulfur dioxide (SO2) in the gas phase for FAIMS analysis. It allows sulfites with a concentration down to 1 mg kg−1 to be easily detected by this new method in 20 min, much shorter than with current technologies. The limits of detection (LOD) and limits of quantification (LOQ) are 1 mg kg−1 and 3 mg kg−1 in HMs, respectively. The new method is of great significance to ensure medical safety and for HM production quality control.
研究领域:
分析化学
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药学
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食品安全
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2021
·
IF 4.098
Zichen Yang,Chaoqun Ma,Jiao Gu,Yamin Wu,Chun Zhu,Lei Li,Hui Gao,Wenzhi Yin,Zirui Wang,Ye Zhang,Yunpeng Shang,Chengwei Wang,Guoqing Chen
Benzoic acid, which has a pivotal role in food additive, is prohibited to add as a preservative in dairy products. China, Brazil, and other countries have proposed standard methods to detect the addition of benzoic acid in food. Surface-enhanced Raman scattering (SERS) is an upcoming spectral detection technique, which has been widely used in the field of material analysis with the advantages of non-invasive, fast detection speed and complex environment with little interference. To detect the illegal use of benzoic acid in dairy industry, we developed Ag-COF (covalent-organic framework) material as SERS substrate to detect benzoic acid in liquid milk. The great enhancement ability of Ag-COF substrate is controlled by the addition of acetic acid and complex interplay between COF material and benzoic acid. This detection method has high sensitivity and reliability that allows us to achieve limit of detection (LOD) of 0.13 μg/mL in milk and 0.00372 μg/mL in water by applying this method. In experiment on recovery rate of real samples, the detection time is less than 15 minutes and the relative standard deviation (RSD) ranged from 2.82% to 5.69%. Therefore, this method has practical significance of the detection of benzoic acid in dairy products.
研究领域:
食品科学
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分析化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
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2022
·Vol. 27
·期 19
·6749
·
IF 4.927
Zhuang Xiang,Yuxiang Jiang,Chen Cui,Yuanping Luo,Zhili Peng
In this study, C-dots were facilely synthesized via microwave irradiation using citric acid and ethylenediamine as carbon precursors. The fluorescence emissions of the C-dots could be selectively quenched by Fe3+, and the degree of quenching was linearly related to the concentrations of Fe3+presented. This phenomenon was utilized to develop a sensitive fluorescence assay for Fe3+detection with broad linear range (0–250, 250–1200 μmol/L) and low detection limit (1.68 μmol/L). Most importantly, the assay demonstrated high reliability towards samples in deionized water, tap water and lake water, which should find potential applications for Fe3+monitoring in complicated environments.Keywords:Fe3+;fluorescence sensing;carbon dots;lake water;limit of detection
研究领域:
环境科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2025
·
IF 11.3
Shengjie Hu,Rumeng Zhao,Yuchen Tang,Tingjuan Gao
Soil and groundwater contamination by lead and cadmium poses significant environmental and health risks, necessitating the development of rapid and accurate field monitoring techniques, which are currently lacking. Although solid-phase colorimetric microProbing imaging offers a promising solution, fine-tuning the hydrophilic/hydrophobic microenvironment of lead or cadmium molecular sensors within the microProbing material is essential for achieving rapid and accurate detection. Here, we report a method to covalently bond xylenol orange (a lead sensor) or physically adsorb dithizone (a cadmium sensor) onto the hydrophilic or hydrophobic entities of TentaGel resins, enabling uniform sensor loading, selective sequestration, and rapid microProbing of soil and groundwater lead or cadmium. Through microenvironment fine-tuning, the method achieved high sensitivity, with detection limits of 28 ppm and 9.9 ppb for soil and groundwater lead, and 50 ppb and 6.8 ppb for soil and groundwater cadmium, respectively. It accurately detected soil and groundwater lead from an economic development zone in Shenzhou (Hebei Province) and cadmium from a mine in Daye (Hubei Province), China. A portable microProbing device was also custom-designed and fabricated. With this device, the microProbing method shows great promise for field monitoring of other heavy metals in contaminated lands.
研究领域:
环境科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL PHYSICS LETTERS
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2025
·
IF 3.1
Yingying Kou,Zhuang Wu,Chenling Yan,Jinggang Wang,Fan Liu,Donghai Yuan
Development of a new sensor for the simple detection of Hg 2+ , which improves on the shortcomings of conventional mercury detection.The size of the nanogold(AuNPs) particles was controlled by controlling the amount of sodium citrate and gold salt. AuNPs was also functionalized by arginine and melamine. The functionalized nanogold was sensitive and selective for Hg 2+ . It is also useful in real water samples. The 32 nm nanogold modification had the lowest limit of detection (LOD). The melamine-functionalized 32 nm AuNPs had a LOD of 4.17 μmol/L, which was better than the arginine-functionalized 32 nm AuNPs with a LOD of 5 μmol/L.
研究领域:
分析化学
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环境科学
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产品关联
DOI
高影响因子
Journal of Inorganic and Organometallic Polymers and Materials
·
2025
·
IF 4.9
Liu Deyan,Zhu Manying,Zhang Nanhai,Song Qinghe,He Guangjun,Wang Shixing,Zhang Libo
It is of urgent significance to develop highly stable and efficient CrO 4 2− detection technology in the fields of drinking water distribution and sewage treatment. A highly stable europium metal-organic framework (Eu-MOF) was designed as fluorescent probe for the efficient detection of trace CrO 4 2− in water solutions. This was achieved through the self-assembly of the lanthanide Eu 3+ and a rigid conjugated organic ligand [2,2’- biquinoline]-4,4’-dicarboxylic acid (H 2 L) under hydrothermal conditions. The obtained Eu-MOF exhibits excellent water stability, thermal stability and photostability. The Eu-MOF can quantitatively detect trace amounts of CrO 4 2− through fluorescence quenching effect. Its detection sensitivity is 4.48 × 10 4 M − 1 , with a detection limit as low as 0.06 µM (9.7 µg/L). This is one of the lowest values for the detection limit of CrO 4 2− in aqueous solution and is far below the allowable level for drinking water specified by World Health Organization. The Eu-MOF sensor also demonstrates excellent selectivity, anti-interference ability, repeatability (after 4 cycles), reproducibility and practical feasibility. Furthermore, the fluorescent luminescence and senseing mechanism were studied in detail. The fluorescence senseing mechanism of the Eu-MOF for CrO 4 2− detection involves the competitive absorption of excitation energy. The fluorescence-based sensing technology using the Eu-MOF provides a reference for environmental pollution detection and holds potential practical value.
研究领域:
环境科学
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材料科学
·
分析化学
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产品关联
DOI
高影响因子
NANO
·
2017
·
IF 1.025
Xiqing Liu,Xiao Wei,Yeqing Xu,Hongji Li,Kai Lu,Kun Wang,Yongsheng Yan
In this paper, a novel fluorescent nanoswitch based on carbon dots (CDs) was developed for the sensitive and selective determination of Hg 2 + and I − . The CDs were obtained by simple hydrothermal process and had a strong fluorescence emission at 440 nm. The fluorescence of the CDs can be selectively quenched by Hg 2 + ion, and then the I − was added into the system, which can interact with Hg 2 + and recover fluorescence of the CDs. Under optimal conditions, the quenching fluorescence intensity on addition of Hg 2 + has obtained a satisfactory linear relationship covering the linear range of 0–50 μ M with the linear relationship ( R 1 2 = 0 . 9 9 9 1 ), and the limit of detection is 0.047 μ M. The additions of I − could lead to the fluorescence intensity of the solution of CDs and Hg 2 + (50 μ M) recover rapidly, which is linearly related ( R 2 2 = 0 . 9 9 5 7 ) to the concentration of I − in the range from 0 to 70 μ M, the detection limit for I − was calculated to be 0.084 μ M. Moreover, the developed method to detect Hg 2 + and I − was evaluated in real examples, and the fluorescence switching can sensitively and selectively detect Hg 2 + and I − over some potentially interfering ions, the recoveries were up to 97.8–107.0% and 96.7–106.6%, respectively.
研究领域:
分析化学
·
环境科学
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产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2024
·
IF 4
Hao Hu,Lei Xue,Yelin Yao,Linlong Deng,Haibin Wang
A novel fluorescent probe, WH , was successfully designed and synthesized. WH demonstrated continuous detection capabilities for Cu 2+ and EDTA in the HEPES system. Specifically, the introduction of copper ions resulted in fluorescence quenching, while the addition of EDTA led to the recovery of fluorescence. The detection limits of WH for Cu 2+ and EDTA were calculated to be 29.66 and 172.13 nmol· L −1 , respectively. Moreover, WH exhibited AIE property in THF/H 2 O system. According to the Job's plot, 1 H NMR titration and mass spectrometry, WH could form a complex with Cu 2+ in a 1:1 ratio, and the combinding constant was 8.2 × 10 3 L mol −1 . WH exhibited outstanding selectivity, high sensitivity, large Stokes shift and strong anti-interference ability. Furthermore, WH had been successfully applied in the detection of Cu 2+ in beer and red wine, showcasing its promising potential for practical application.
研究领域:
分析化学
·
材料科学
·
环境科学
✓ 已验证
DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2025
·
IF 4.8
Guangwei Zhao,Shengshuo Zhang,Yujie Zou,Nan Jia,Liyuan Zheng,Jinhua Dong
Introduction: Testosterone is a steroid hormone, which plays a pivotal role in regulating metabolism and protein synthesis in the body. The detection of testosterone is of paramount importance for diagnostic purposes in clinical settings, as well as for monitoring athletes’ physiological parameters and ensuring the integrity of sports competitions.Methods: Herein, we present a phage display-derived biosensing platform through genetic engineering of the TS77 antibody variable domains. The variable region genes of the heavy and light chains from TS77 antibody were cloned into the pDong1 plasmid and displayed on the phage surfaces through phage display technology. Subsequently, a novel non-competitive open-sandwich ELISA (OS-ELISA) was developed for testosterone detection, leveraging the antigen-induced interaction changes in antibody variable regions.Results: OS-ELISA based on anti L-chain antibody achieved a limit of detection (LOD) of 2.71 nM and a half-maximal effective concentration (EC50) of 0.22 μM for testosterone detection. Furthermore, the enhanced OS-ELISA platform incorporating purified maltose binding protein fused with VL (MBP-VL) and VH phage demonstrated a LOD of 1.07 pM and a wide working range from 1 pM to 10 mM.Discussion: The OS-ELISA developed in this study exhibits high sensitivity and a broad dynamic range for testosterone quantification, showing significant potential for clinical diagnostics and athlete monitoring applications.
研究领域:
生物化学
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免疫学
·
临床诊断
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DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2022
·
IF 4.52
Xiaobin Lin,Chengyi Hong,Zhengzhong Lin,Zhiyong Huang
Histamine is an organic nitrogen-containing compound produced by the decarboxylation of histidine and is toxic in high intakes. Therefore, Histamine detection is an important work for food safety. In the present study, a rapid detection method for histamine in fish was established based on the fluorescence activity of graphite phase carbon nitride (g-C 3 N 4 ). After calcinating the mixture of melamine , potassium chloride , and sodium chloride, g-C 3 N 4 was obtained with strong fluorescence and good dispersity in solutions. The strong blue fluorescence of g-C 3 N 4 at 420 nm was fairly selectively quenched by histamine with linear range from 5 μmol L −1 to 800 μmol L −1 , and the detection limit was 0.61 mg kg −1 (3σ/K, n = 9). The fluorescent method was applied to histamine detection in different kinds of fish, and the data were validated by the method of high-performance liquid chromatography. The results indicated that the established fluorescent method was suitable for the rapid detection of histamine in fish with high accuracy, sensitivity, and stability.
研究领域:
食品安全
·
分析化学
·
生物化学
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DOI
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2024
·
IF 4.1
Bowen Zha,Xinhui Huang,Liangbin Lin,Hongyu Guo,Fafu Yang
Although some detection methods for folic acid have been developed based on the conventional instruments, the rapid, simple and in-situ detection for folic acid was rarely reported. In this work, a “turn-on” and “color-changing” fluorescence sensor ( TBCS ) on detecting folic acid was designed and synthesized. TBCS emits the green fluorescence, which transferred to the strong blue fluorescence after binding folic acid. The high sensing selectivity was observed among various ions and biomolecules. The detection limitation of TBCS for folic acid was 4.32 × 10 −7 M. The sensing mechanism was proposed as that the ICT effectiveness of TBCS was interrupted by sensing folic acid. The application experiments on test paper, folic acid tablets and simulated sample displayed the good in-situ detecting ability of TBCS for folic acid, suggesting the good application prospect on rapid, simple and in-situ detecting folic acid in real environments.
研究领域:
分析化学
·
生物医学
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DOI
高影响因子
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