FOOD CHEMISTRY
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2023
·
IF 8.8
Zihua Guo,Bin Yang,Jian Zhu,Shuyan Lou,Huimin Hao,Weiyi Lu
The intake of estradiol residue from food will lead to health problems, so the rapid and reliable detection of estradiol residue is essential. Multi-mode assays are inherently self-correcting and self-validating, providing more reliable, interference-resistant, high-fidelity results. Here, we developed a dual-mode method to achieve a rapid, reliable, and sensitive detection of estradiol. The binding of thioflavin T to the cavity sites of estradiol aptamer not only generates a strong fluorescence signal, but also provides light-activated oxidase activity to produce a blue oxidation product. But the specific binding between aptamer and estradiol will compete with the above process. Thus a dual-mode fluorescence and colorimetric detection of estradiol was realized with a detection limit of 0.15 μM or 0.27 μM. Besides, the dual-mode method showed a good selectivity for estradiol detection. The method could detect estradiol in meat food, showing a good application potential to monitor endocrine-disrupting chemical.
研究领域:
生物化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF SOLID STATE CHEMISTRY
·
2021
·
IF 3.498
Han Zhang,Guan-Yu Ding,Afifa Yousaf,Li Chen,Xin-Long Wang,Guo-Gang Shan,Chun-Yi Sun,Zhong-Min Su
Luminescent sensors have received extensive attention due to their fast-response and easy-operation and when integrating multifunctions in a sensor, the cost will be reduced substantially. Covalent organic polymers (COPs) as multifunctional luminescent sensor with outstanding performance are still underexplored. In this work, a typical COP named as COP-2 show preponderant performance in the detection of Fe 3+ , MnO 4 − , and TNP. COP-2 has the highly efficient ability to selectively detect Fe 3+ among the other metal ions , and the limit of the detection (LOD) is 0.89 μ M, which outperforms the reported covalent organic materials. Anion sensing shows the COP-2 has the highly anti-interference ability and recyclicity to detect MnO 4 − . Explosive analogs sensing demonstrates that COP-2 owns the superior ability in sensing TNP in the knockdown concentration with 0.0067 ppm LOD, which superior to the values in the reported literature. In addition to the above superb sensing performance, COP-2 can be utilized as a white light-emitting diode (WLED) when combined with the commercial blue emission LED and presents a Commission Internationale de l’Eclairage (CIE) coordinate of (0.31, 0.32). These results show the COP-2 not only has an appealing performance in multifunctional luminescent sensing application, but also has a possible application in white light emitting.
研究领域:
材料科学
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化学传感
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光学器件
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Hui Dong,Weitian Chen,Ke Xu,Linlin Zheng,Bingyu Wei,Ruiyu Liu,Jingru Yang,Tao Wang,Yanli Zhou,Yintang Zhang,Maotian Xu
Parkinson’s disease (PD) is a progressive neurodegenerative disorder characterized by the loss of dopaminergic neurons and the accumulation of alpha-synuclein. Glutathione (GSH), a key antioxidant, is significantly depleted in PD patients. This study presents a dual-mode detection strategy for selectively determining GSH using a single probe. A series of “turn-on” electrochemical and fluorescent probes were developed, with resorufin (Re) serving as the reporting unit and featuring specific GSH recognition sites. Among these, the 7-(3,5-dinitrophenoxy)-3H-phenoxazin-3-one (Re-DNP) probe was selected for its high selectivity as both a fluorescent and electrochemical probe. Its response to GSH was superior in comparison to that observed for hydrogen sulfide (H2S) and cysteine (Cys). For electrochemical detection using screen-printed carbon electrode (SPCE)/carbon nanotube (CNT) modified electrodes, the detection limit for GSH was 5 μM, with a linear range of 25–500 μM. In fluorescence detection, the probe produced a 78-fold increase in emission at 630 nm in the presence of GSH, with a strong linear correlation between fluorescence intensity and GSH concentration in the range of 10–700 μM, and a detection limit of 2 μM. When applied to real clinical serum samples, the probe demonstrated significantly lower GSH levels in both PD mice and human patients compared to healthy controls. This dual-mode detection method provides a sensitive and accurate tool for GSH detection, with potential applications in understanding GSH’s role in PD and related neurodegenerative diseases.
研究领域:
医学
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神经科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Xiaoyun Bi,Manzhao Yao,Zhaoyan Huang,Zuhao Wang,Huahao Shen,Ching-ping Wong,Can Jiang
Electronic skin (e-skin) is showing a huge potential in human–computer interaction, intelligent robots, human health, motion monitoring, etc. However, it is still challenging for e-skin to realize distinguishable detection of stretching strain, vertical pressure, and temperature through a simple noncoupling structure design. Here, a stretchable multimodal biomimetic e-skin was fabricated by integrating layer-by-layer self-assembled crumpled reduced graphene oxide/multiwalled carbon nanotubes film on natural rubber (RGO/MWCNTs@NR) as stretchable conductive electrodes and polyacrylamide/NaCl ionogel as a dielectric layer into an ionotropic capacitive mechanoreceptor. Unlike natural skin receptors, the sandwich-like stretchable ionogel mechanoreceptor possessed a distinct ionotropic capacitive behavior for strain and pressure detection. The results showed that the biomimetic e-skin displayed a negative capacitance change with superior stretchability (0–300%) and a high gauge factor of 0.27 in 180–300% strain, while exhibiting a normal positive piezo-capacitance behavior in vertical pressure range of 0–15 kPa with a maximal sensitivity of 1.759 kPa–1. Based on this feature, the biomimetic e-skin showed an excellent synchronous detection capability of planar strain and vertical pressure in practical wearable applications such as gesture recognition and grasping movement detection without a complicated mathematical or signal decoupling process. In addition, the biomimetic e-skin exhibited a quantifiable linear responsiveness to temperature from 20–90 °C with a temperature coefficient of 0.55%/°C. These intriguing properties gave the biomimetic e-skin the ability to perform a complete function similar to natural skin but beyond its performance for future wearable devices and artificial intelligence devices.
研究领域:
材料科学
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生物医学工程
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传感器技术
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产品关联
DOI
PubMed
高影响因子
MEASUREMENT SCIENCE and TECHNOLOGY
·
2024
·Vol. 36
·期 1
·015903
·
IF 2.7
Qingqing Wang,Yu Pan,Yanyan Wang,Kaikai Zhang,Shengzhe Shi,Sheng Liu
Traditional electrochemical workstations are costly, complex, bulky, and primarily used in laboratories. This study develops a reliable, practical, and cost-effective portable wireless potentiostat to achieve real-time detection on-site and overcome the limitations of traditional electrochemical workstations. The system employs a general-purpose microcontroller unit, a dual-mode bluetooth module and cost-effective multi-analog-to-digital converter to achieve differential sampling of the LMP91000. The system is equipped with buttons and OLEDs, enabling connection to mobile phones and computers for in-depth data analysis or independent operation. The system was successfully tested with [Fe(CN)6]3−, C6H12O6, and C3H6O3 solutions at concentrations ranging from 0 to 20 mm. The goodness-of-fit (R2) values are 0.984, 0.996, and 0.998, respectively. The average relative standard deviation of the three blank solutions is approximately 3.22%. The detection limits measured (0.003, 0.009, and 0.005 mm) are all lower than the minimum detection concentration (0.2, 0.1, and 0.1 mM). The coefficient of variation for repetitive experiments is less than 5.53%. The device accurately executed chronoamperometry (applied voltage range is ± 1.2 V, current range is ± 882 μA, accuracy is ±1%) with high sensitivity and good repeatability. Based on this circuit, a lactic acid detector and a urine glucose detector were developed, which work stably and support long-term operation, proving the stability and reliability of the circuit. Compared to commercial electrochemical workstations, portable wireless electrochemical system offers remarkable advantages in cost (<$6.4), size (41.5 mm × 76.5 mm), and practicality, making it suitable for a range of applications, including biomedical analysis, food safety, environmental monitoring, and smart wearables.
研究领域:
生物医学工程
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分析化学
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产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
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2022
·Vol. 46
·期 27
·13021-13029
·
IF 3.925
Xinlei Zhang,Peng Liu,Bohan Li,Xianliang Li,Yan Xu
Hydrogen-bond driven self-assembly of carbon dot-based luminescent materials has been widely studied in the fields of sensor and optoelectronic devices. However, the uniform confinement of carbon-dots (CDs) into crystalline porous materials through hydrogen bond interactions has rarely been reported. Herein, an O-phenylenediamine (O-PD) mediated Zr-based MOF material denoted as CDs@UiO-66(COOH)2 was successfully synthesized via an efficient solvent-free synthetic method. The O-PD molecules were grafted to the structural pyromellitic acid ligands in the UiO-66(COOH)2 framework via H-bonding interactions, and during the subsequent thermal treatment process the O-PD molecules were partially decomposed and converted into luminescent CDs. The resultant CDs@UiO-66(COOH)2 composites retained the crystallinity of UiO-66(COOH)2, and the confined CDs in UiO-66(COOH)2 show excitation-independent stable green emitting properties when dispersed in a variety of solvents. The CDs@UiO-66(COOH)2 composite exhibits excellent chlortetracycline detection performance with high selectivity, anti-interference and low limit of detection (LOD = 0.102 μM) in a wide linear range of 0–100 μM. Also, it shows an excellent stimuli-response in fluorescence intensities toward pH and temperature changes. Acting as an optical thermometer, the maximum relative temperature sensitivity (Sr) is 0.82% °C−1 in the range of 10–80 °C. This work opens a new way for exploring fluorescent CD-integrated MOF composites via a hydrogen-bond driving strategy for multifunctional fluorescent sensing applications.
研究领域:
材料科学
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分析化学
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传感技术
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Yuping Zeng,Xiangyu Gan,Zhourui Xu,Xiaoxiang Hu,Chenxuan Hu,Hanbin Ma,Hangjia Tu,Bao Chai,Chengbin Yang,Siyi Hu,Yujuan Chai
Background Sensitive and rapid antigen detection is critical for the diagnosis and treatment of infectious diseases, but conventional ELISAs including chemiluminescence-based assays are limited in sensitivity and require many operation steps. Fluorescence immunoassays are fast and convenient but often show limited sensitivity and dynamic range. Results To address the need, an aggregation-induced emission fluorgens (AIEgens) enhanced i mmunofluorescent assay with beads-based quantification on the digital microfluidic (DMF) platform was developed. Portable DMF devices and chips with small electrodes were fabricated, capable of manipulating droplets within 100 nL and boosting the reaction efficiency. AIEgen nanoparticles (NPs) with high fluorescence and photostability were synthesized to enhance the test sensitivity and detection range. The integration of AIEgen probes, transparent DMF chip design, and the large magnetic beads (10 μm) as capture agents enabled rapid and direct image-taking and signal calculation of the test result. The performance of this platform was demonstrated by point-of-care quantification of SARS-CoV-2 nucleocapsid (N) protein. Within 25 min, a limit of detection of 5.08 pg mL −1 and a limit of quantification of 8.91 pg mL −1 can be achieved using <1 μL sample. The system showed high reproducibility across the wide dynamic range (10–10 5 pg mL −1 ), with the coefficient of variance ranging from 2.6% to 9.8%. Significance This rapid, sensitive AIEgens-enhanced immunofluorescent assay on the DMF platform showed simplified reaction steps and improved performance, providing insight into the small-volume point-of-care testing of different biomarkers in research and clinical applications.
研究领域:
生物医学工程
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分析化学
·
分子诊断
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Yu Fan,Jie Yao,Mengke Huang,Chenxi Linghu,Jinlin Guo,Yang Li
Due to the biochemical and pharmacological activities, the convenient and effective detection of quercetin (Qc) is very important for biochemistry, pharmaceutical chemistry and clinical medicine. A kind of non-conjugated polymer dots (NCPDs) was used as a versatile and sensitive dual-mode optical output for Qc detection, which was synthesized by hyperbranched poly(ethylenimine) (PEI) and l -threonine via environmentally friendly way. The dual-mode method proposed in this work had high sensitivity and definite selectivity for Qc detection. Additionally, it was convenient for the naked eyes to observe the fluorescence brightness and color change.
研究领域:
分析化学
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生物医学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SYNTHETIC METALS
·
2016
·
IF 2.299
Li Yuan Qin,Ling Jie Hou,Jia Feng,Jian Bin Chao,Yu Wang,Wei Jun Jin
A reversible fluorescent-colorimetric Schiff-base receptor (L) derived from julolidine and aminourea was developed for the selective sensing of Al 3+ ions in buffered aqueous medium. Sensor L demonstrated the presence of Al 3+ ions by both fluorescence enhancement and an instantaneous color change of solution from colorless to yellow. L forms complex with Al 3+ in 1:1 ratio as supported by Job’s plot analysis and mass spectroscopic measurement. The sensitivity of the fluorescent based assay (4.67 nM) for Al 3+ is far below the WHO acceptable limit (7.41 μM) in drinking water. Furthermore, L was employed for imaging the Al 3+ ion in living biological cells.
研究领域:
分析化学
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生物医学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2023
·
IF 4.1
Bangjie Yin,Zheng Zhang,Yingchun Wang,Hui Zeng,Jin Xu,Hongzhou Li,Yan Li,Min Zhang
The growing demand for tailored detectors in capillary electrophoresis (CE), addressing tasks like field deployment or dual-detection analysis, emphasizes the necessity for compact detection cells. In this work, we propose cost-effective and user-friendly additive manufacturing (3D-printing) approaches to produce such miniaturized detection cells suitable for a range of CE applications. Firstly, capacitively-coupled contactless conductivity detection (C 4 D) cells of different sizes are fabricated by casting low-melting-point alloy into 3D-printed molds. Various designs of Faraday shields are integrated within the cells and compared. A mini-C 4 D cell (9.5×7.0×7.5 mm 3 ) is produced, with limits of detection for alkaline cations ranging from 8-12 μM in a short–capillary based CE application. Secondly, ultraviolet photometric (UV-PD) detection cells are fabricated using 3D printing. These cells feature two narrow slits with a width of 60 μm, which are positioned along the path of incident and transmission light to facilitate collimation. A deep UV-LED (235 nm or 255 nm) is employed as the light source, and black resin is determined to be the optimal material for 3D printing the UV-PD cell, owing to its superior UV light absorption capabilities. The UV-PD cell is connected to the LED and photodetector through two optical fibers, making it easy to switch the light source and detector. The effective pathlength and stray light percentage for detecting on a 75 μm id capillary are 74 μm and 0.5 %, respectively. Thirdly, a dual-detection cell that combined C 4 D and UV-PD at a single detection point is proposed. The performance of direct detection by C 4 D and indirect detection by UV-PD is compared for detecting organic acids. The strategies for developing cost-effective compact detection cells facilitate the versatile integration of multiple detection methods in CE analysis.
研究领域:
分析化学
·
生物分析
·
仪器科学与技术
✓ 已验证
DOI
PubMed
高影响因子
Materials Advances
·
2024
·
IF 5.2
Premkumar Sellan,Manigandan Selvan,Abida Perveen,Din Nasrud,Sakthivel Chandrasekar,Pitchaikannu Venkatraman,Devaraj Nataraj,Byung Seong Bae,Damian Chinedu Onwudiwe,Lei Wei
Perovskite materials are potential photo-absorbers, and their solution-processed perovskite layers are widely reported in photodetection devices. However, defects in perovskites pose a serious challenge to device performance. One possible way to overcome this set-back is growing a perovskite single crystal and deploying it in photon detection devices. Herein, we report the growth of pure and Bi-doped CsPbBr3 perovskite single-crystals and their high-energy photodetection characteristics. The Bi-doped CsPbBr3 perovskite single-crystals exhibited good photodetection characteristics in the visible, X-ray and gamma-ray regions. Under an illumination of 470 and 530 nm, the Bi-doped CsPbBr3 perovskite single-crystal photodetector displayed a linear response for the biasing voltage ranging from 2 V to 5 V. The device displayed a higher magnitude of photocurrent and exhibited a better ON/OFF ratio of ∼596 at λ = 470 nm and ∼24 at λ = 530 nm. The gamma ray detection properties of the Bi-doped CsPbBr3 perovskite single crystal were studied using two different radioactive sources with varying activity levels. A characterization study using a portable X-ray device revealed an excellent response to varying tube voltages and currents. The present study reveals the versatility of the Bi-doped CsPbBr3 perovskite single-crystals in photon detection under a wide range of illumination.
研究领域:
材料科学
·
辐射检测
·
光电子学
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Xiaohui Wang,Yuanxiangyi Feng,Zhe Li,Jinhua Liu,Dandan Sun,Wei Yang,Xiaowei Xing,Wenjun Liu,Hongxin Zhang
A fluorescent capillary sensor for detecting the bioanalytically and biologically relevant analytes pH, O 2 , and CO 2 has been designed. The sensor is based on single capillary tube with 2 mm inner diameter, which is simultaneous doped with emissive O 2 -sensitive indicator Ru(dpp) 3 (PF6) 2 (RuDP), pH-sensitive and CO 2 -sensitive indicator 8-hydroxypyrene-1, 3,6-trisulfonic acid trisodium salt (HPTS). The multifunctional sensor can be excited at the same wavelength and realize multi-parameter optical monitoring at the different sensing position. Studies in phosphate-buffered solutions display the excellent feasibility of the capillary sensor for fluorescence detection of pH, O 2 , and CO 2 . Overall, the multifunctional capillary sensor exhibits great potential in real-time blood gas analysis, and clinical application of multi-parameter biological detection.
研究领域:
生物分析学
·
医学工程
·
化学传感
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）