JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2022
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IF 5.895
Zhen Li,Qiang Huang,Yu Zheng,Yong Zhang,Xin Li,Shiqing Zhong,Zhijiang Zeng
Identifying the components of Camellia oleifera honey and pollen and conducting corresponding toxicological tests are essential to revealing the mechanism of Camellia oleifera toxicity to honey bees. In this research, we investigated the saccharides and alkaloids in honey, nectar, and pollen from Camellia oleifera, which were compared with honey, nectar, and pollen from Brassica napus, a widely planted flowering plant. The result showed that melibiose, manninotriose, raffinose, stachyose, and lower amounts of santonin and caffeine were found in Camellia oleifera nectar, pollen, and honey but not in B. napus nectar, pollen, and honey. Toxicological experiments indicated that manninotriose, raffinose, and stachyose in Camellia oleifera honey are toxic to bees, while alkaloids in Camellia oleifera pollen are not toxic to honey bees. The toxicity mechanism of oligosaccharides revealed by temporal metabolic profiling is that oligosaccharides cannot be further digested by honey bees and thus get accumulated in honey bees, disturbing the synthesis and metabolism of trehalose, ultimately causing honey bee mortality.
研究领域:
昆虫学
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毒理学
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植物科学
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农业科学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
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IF 7.7
Yudie Wang,Hang Gao,Xin Wang,Dawei Li,Wen Zhang,Xuan Meng,Lijie Wang,Tao Zhu,Peijie Dong,Yao Chen,Xin Meng
A series of intricate and dynamic physiological healing processes are involved in the healing of skin wounds. Herein, a multifunctional hydrogel is firstly designed and constructed by L -arginine-grafted O -carboxymethyl chitosan (CMCA), catechol-modified oxidized hyaluronic acid (DOHA), and dopamine nanoparticles (pDA-NPs). pDA-NPs were loaded in hydrogel for inherently powerful antimicrobial properties and could be as a cross-linking agent to construct hydrogels. Raffinose (Raf) was further incorporated to obtain CMCA-DOHA-pDA2@Raf hydrogel for its function of modulating epidermal differentiation. The hydrogel has good physicochemical properties and could promote cell proliferation and migration, which shows superior hemostatic capabilities in animal models of hemorrhage. The hydrogel significantly promoted wound healing on rat skin defect models by upregulating VEGF and CD31 and decreasing IL-6 and TNF-α, stimulating neovascularization and collagen deposition in epithelial structures. This multifunctional hydrogel implies the potential to be a dynamic wound dressing.
研究领域:
生物材料
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伤口愈合
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纳米技术
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组织工程
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药学
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PubMed
高影响因子
BMC MUSCULOSKELETAL DISORDERS
·
2024
·Vol. 25
·期 1
·1-13
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IF 2.2
Xu Kuishuai,Zhang Liang,Wang Tianrui,Yu Tengbo,Zhao Xia,Yu Ning,Zhang Yingze
Objective To assess the mechanism of supraspinatus tendinopathy induced by type 2 diabetes mellitus (T2DM) in rats using untargeted metabolomics analysis. Methods The liquid chromatography-mass spectrometry (LC-MS)-based untargeted metabolomics approach was used to screen tendon biomarkers of supraspinatus tendinopathy in rats with T2DM. Seventy-eight Sprague-Dawley rats were divided into normal group (NG) and T2DM groups. Rats in T2DM groups were divided into 12-week (T2DM-12w), and 24-week (T2DM-24w) subgroups according to the time point of the establishment of the T2DM rat model. Histological evaluation (modified Bonar score) and biomechanical testing were used to analyze the adverse effects of type 2 diabetes on the tendon of the supraspinatus muscle in rats.Three comparable groups were set up, including T2DM-12w group vs. NG, T2DM-24w group vs. NG, and T2DM-24w group vs. T2DM-12w group. Differentially expressed metabolites (DEMs) in the supraspinatus tendons in the three groups of rats were analyzed using LC-MS, and data were analyzed using multivariate statistical methods to screen potential biomarkers. The DEMs included in the intersection of the three groups were identified as those associated with the development of diabetic supraspinatus tendinopathy, and trend analysis and pathway topology analysis were performed. Results With the progression of diabetes, the tendinopathy of the supracinatus muscle of diabetic rats gradually intensified, mainly manifested as inflammatory reactions, disordered collagen fibers, fat infiltration, and increased modified Bonar score. The intersection of DEMs among the three comparable groups was resulted in the identification of 10 key DEMs, in which melezitose and raffinose showed a continuous increasing trend with the prolongation of disease course. By pathway topology analysis, 10 DEMs ( P < 0.01) were mainly associated with the pathways of galactose metabolism, which could be involved in the development of diabetes-induced supraspinatus tendinopathy. Conclusion T2DM causes tendinopathy of the supraspinatus muscle in rats. 10 key DEMs obtained by untargeted metabolomics assay suggested that the development of diabetes-induced supraspinatus tendinopathy was associated with changes in metabolic pathways, such as galactose metabolism. melezitose and raffinose hold promise as a biomarker for disease discrimination and/or disease indication in diabetic supraspinatus tendinopathy.
研究领域:
代谢组学
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糖尿病研究
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肌肉骨骼疾病
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动物模型
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Hongyu Pan,Mengtao Cheng,Zhenxing Li,Xiaomei Sun,Chunchao Han
Here, we optimized the active units of polysaccharides and investigated the conformational relationship between the polysaccharides and alcoholic liver disease (ALD) at the molecular level. We used data mining to screen polysaccharide structural parameters for ALD (PSP-ALD). Most ALD-resistant polysaccharides against ALD comprised glucose (Glc), mannose (Man), galactose (Gal), arabinose (Ara), and rhamnose (Rha). Additionally, (1 → 6)-, (1 → 3)-, and (1 → 4)- glycosidic linkages were mainly contained. Polysaccharides against ALD have a wide molecular weight distribution (2.1 × 10 3 Da - 9.6 × 10 7 Da). Based on the PSP-ALD analysis, six commercially available oligosaccharides were selected and their structures were built. After molecular docking, the binding affinities between stachyose and the key ALD targets were stronger, indicating that stachyose may be a polysaccharide-active unit against ALD (PAU-ALD). Furthermore, histological examination of liver tissue combined with serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and triglycerides (TG) showed that stachyose had a significant protective effect against ALD in mice. In summary, we optimized a PAU-ALD and developed a method for studying the structure-activity relationship between polysaccharides and ALD at the molecular level, which provides a new research direction for the development and utilization of polysaccharides and their clinical applications in ALD.
研究领域:
分子生物学
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药理学
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生物信息学
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医学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
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IF 8.8
Yuanyuan Li,Bingxue Liu,Xiaoxue Zhang,Yanjie Liu,Siying Wang,Shujun Li,Xiuhua Zhao
A new amphiphilic oligosaccharide derivative, based on lutein modification onto the OH position of stachyose with facile and mild esterification, was prepared and used to improve the oral bioavailability of lutein. The structures of lutein–stachyose derivative (LS) were confirmed by Fourier transform infrared spectroscopy and hydrogen-1 nuclear magnetic resonance, indicating that one stachyose is connected to one lutein through succinic acid. The critical micelle concentration of LS was approximately 6.86 ± 0.24 mg/mL, corresponding to the free lutein concentration of approximately 2.96 mg/mL. LS has better digestive stability and free radical scavenging ability, and it could inhibit the degradation of lutein in the gastrointestinal tract. Importantly, LS is nontoxic to cells and zebrafish embryos. In terms of oral bioavailability in rats, the AUC 0-12h values of LS were 2.26 times higher than those of free lutein. Therefore, stachyose modification is a promising strategy for improving the oral bioavailability of fat-soluble lutein.
研究领域:
药学
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营养保健品
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化学
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药理学
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生物技术
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DOI
PubMed
高影响因子
Science Bulletin
·
2023
·
IF 18.9
Yue Zhou,Jiayuan Huang,Guanlin Wang,Zizhao Zhai,Maizbha Uddin Ahmed,Xiao Xia,Cenfeng Liu,Yuzhen Jin,Xin Pan,Ying Huang,Chuanbin Wu,Xuejuan Zhang
Pulmonary infections caused by multidrug-resistant bacteria have become a significant threat to human health. Bacterial biofilms exacerbate the persistence and recurrence of pulmonary infections, hindering the accessibility and effectiveness of antibiotics. In this study, a dry powder inhalation (DPI) consisting of polymyxin B sulfate (PMBS) inhalable microparticles and high-lectin-affinity (HLA) sugar (i.e., raffinose) carriers was developed for treating pulmonary infections and targeting bacterial lectins essential for biofilm growth. The formulated PMBS-HLA DPIs exhibited particle sizes of approximately 3 μm, and surface roughness varied according to the drug-to-carrier ratio. Formulation F5 (PMBS: raffinose = 10: 90) demonstrated the highest fine particle fraction (FPF) value (64.86%), signifying its substantially enhanced aerosol performance, potentially attributable to moderate roughness and smallest mass median aerodynamic particle size. The efficacy of PMBS-HLA DPIs in inhibiting biofilm formation and eradicating mature biofilms was significantly improved with the addition of raffinose, suggesting the effectiveness of lectin-binding strategy for combating bacterial biofilm-associated infections. In rat models with acute and chronic pulmonary infections, F 5 demonstrated superior bacterial killing and amelioration of inflammatory responses compared to spray-dried PMBS (F 0 ). In conclusion, our HLA carrier-based formulation presents considerable potential for the efficient treatment of multidrug-resistant bacterial biofilm-associated pulmonary infections.
研究领域:
药剂学
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药理学
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微生物学
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呼吸医学
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DOI
PubMed
高影响因子
Arabian Journal of Chemistry
·
2024
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IF 5.3
Wensen Zhang,Na Cui,Fazhi Su,Yanping Sun,Biao Li,Meng Liu,Yuanning Zeng,Bingyou Yang,Qiuhong Wang,Haixue Kuang
Stachyose (ST), a naturally occurring compound extracted from cucumbers and legumes, holds great promise as a natural therapeutic agent. In this study, the impact of ST on blood deficiency syndrome (BDS) induced by cyclophosphamide (CP) and acetylphenylhydrazine (APH) was investigated in a rat model. Subsequently, the levels of erythropoietin (EPO), granulocyte colony-stimulating factor (G-CSF), tumour necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6) were quantified in rat blood and peripheral blood cells. Furthermore, haematoxylin and eosin (HE) staining was employed to elucidate morphological alterations in the spleen tissue. To gain comprehensive insights into the therapeutic potential and underlying mechanisms of ST against BDS, we used 16S rRNA gene sequencing and serum and spleen metabolomic analyses. Our findings revealed a significant ameliorative effect of ST on BDS. Notably, at the phylum level, Firmicutes and Bacteroidota exhibited marked variations in abundance, while at the genus level, discernible changes were observed in the abundances of Lactobacillus and Bifidobacterium , among others. The therapeutic effects of ST on BDS were proposed to be due to its modulation of phenylalanine metabolism and glycine, serine and threonine metabolism and its impact on the restructuring of the gut microbiota.
研究领域:
药理学
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微生物学
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代谢组学
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分子生物学
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病理学
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DOI
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
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2021
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IF 4.36
Pu-Yang Gong,Yu-Jie Guo,Yu-Shan Tian,Li-Fei Gu,Jin Qi,Bo-Yang Yu
Ethnopharmacological relevance In traditional Chinese medicine (TCM) and modern pharmacodynamics , dried Rehmannia Radix (DRR) possesses prominent anti-thrombotic activity that decreases after processing by nine steaming and drying cycles to develop processed Rehmannia Radix (PRR). Due to the complexity of the DRR components, the chemical mechanism leading to efficacy changes of DRR caused by processing is still unclear. Aim of study This study aimed to trace the anti-thrombotic active compounds of DRR and different degrees of processed RR (PRR) and to evaluate the synergistic effects among different active components. Materials and methods The anti-thrombotic active chemical fraction of DRR extracts was evaluated. Targeted fractions of the processed products of RR were prepared at different processing stages. The changes in monosaccharides , oligosaccharides and secondary metabolites during processing were characterized by multidimensional high-performance liquid chromatography (HPLC). The anti-thrombotic effects of targeted fractions of different RR samples were evaluated by analyzing the length of tail thrombus (LT) and serum biochemical indicators in carrageenan-induced tail-thrombus mice. The spectrum-effect relationships were investigated by partial least squares regression (PLSR) analysis and gray correlation analysis (GRA). Finally, the active compounds were screened by spectrum-effect relationship analysis and validated in vivo , and their synergistic effects were determined by Webb's fraction multiplication method. Results Six ingredients highly associated with anti-thrombotic activities were screened out by the spectrum-effect relationship analysis, of which oligosaccharides (stachyose, sucrose and raffinose) and iridoid glycosides (catalpol, leonuride and melitoside) possessed a synergistic effect on tumor necrosis factors (TNF-α), interleukin 1β (IL-1β) and plasminogen activator inhibitor 1 (PAI-1)/tissue-type plasminogen activator (t-PA) ratio in vivo with synergistic coefficient (SC) > 1. Conclusion The main material basis of the anti-thrombotic activities of DRR is oligosaccharide components of stachyose, raffinose and sucrose, iridoid glycosides components of catalpol , leonuride and melittoside. The two kinds of components exert synergistic anti-thrombotic effects by inhibiting the expression of inflammatory factors and regulating the balance of the fibrinolysis system.
研究领域:
民族药理学
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药理动力学
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传统中药
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化学分析
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血栓研究
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生物统计学
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产品关联
DOI
PubMed
高影响因子
Small
·
2025
·
IF 12.1
Jichun Li,Yu Deng,Luyang Liu,Yidan Chen,Fengluan Jiang,Meihua Chen,Jianfeng Xia,Yonghui Deng
Flexible gas sensing devices have attracted a growing research interest in recent years due to their potential applications in various fields such as wearable electronics. Porous 2D metal oxide semiconductors (2D MOSs) with superior electrical and catalytic characteristics are deemed as promising materials for flexible gas sensors, especially for low-concentration gas detection; however, the synthesis of high-quality 2D MOSs remains challenging. In this study, 2D porous In 2 O 3 nanosheets with a high specific surface area (85.9 m 2 /g) and a thickness of 4.0 nm are synthesized via a scalable micro-explosion assisted caramelization reaction. The synthesis parameters affecting the formation of 2D porous metal oxide nanostructures are systematically studied, and a proposed growth mechanism is dominated by generating the sacrificial 2D carbon intermediate templates. Furthermore, flexible gas sensors are fabricated by depositing 2D porous In 2 O 3 nanosheets on polyimide substrates, which exhibit outstanding sensing performance with high sensitivity toward trace hydrogen sulfide (response value of 17.3 for 250 ppb) at 37 °C, excellent selectivity, and high stability. The sensor is further integrated into a wearable, flexible wireless sensing device, showing good reliability and great potential in detecting trace hydrogen sulfide in practical applications.
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高影响因子
Foods
·
2025
·Vol. 15
·期 1
·70
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IF 5.1
Chenyu Sun,Fei Pan,Wenli Tian,Zongyan Cui,Xiaofeng Xue,Yitian Xu
Honey variety authentication is critical for ensuring market integrity and protecting consumer rights, especially for high-value unifloral honeys, such as acacia honey, which are frequently adulterated with low-value alternatives such as rape honey due to their similar visual appearance. The aim of this study was to develop a method for precise discrimination between rape honey and acacia honey using their chemical profiles combined with machine learning. A total of 542 honey samples were collected from major beekeeping regions in China. Targeted quantification of 12 sugars and 20 amino acids was performed using UPLC-MS/MS. Multivariate analysis revealed significant differences in sugar and amino acid compositions between the two honey types, though partial samples overlapped due to chemical similarity. Six machine learning algorithms, including the Multilayer Perceptron, were employed for classification. Optimization was performed via 10-fold cross-validation and ADASYN oversampling, yielding optimal performance of 98% and 100% prediction accuracies for rape honey and acacia honey, respectively, on the independent test set. SHAP (Shapley Additive Explanations) analysis identified key differential markers, including fructose, turanose, glucose, and GABA, which contributed most to the classification. Furthermore, a user-friendly web application was developed to facilitate rapid on-site authentication. This study provides an innovative technical framework for honey variety discrimination, with potential applications in quality control and anti-fraud practices.
研究领域:
食品科学
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分析化学
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机器学习应用
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养蜂业
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食品真伪鉴别
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PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
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IF 9
Qingsong Liu,Jing Sun,Yingmei Peng,Xi Xu,Zhuo Li,Weiyi Li,Xintao Wei,Xing Sun,Miao Tian
Membrane-based techniques have been playing an important role in desalination. The feed spacer in membrane modules not only separates membrane sheets to form feed channels but also acts as a turbulence promoter, enhancing flow mixing, mass transfer, and thereby reducing concentration polarization. Optimizing feed spacer design is essential for controlling the membrane fouling and lowering the energy consumption. In this study computational fluid dynamics (CFD) was exploited to optimize spacer configurations; 3D printing was employed to fabricate spacer in terms of the novel designs inspired by airplane wing aerodynamics; fluid dynamics experiments were performed to assess their effects on the flow parameters. The relationship between the flow parameters, membrane hydraulic performance, and anti-fouling properties was analyzed to reveal a trade-off between the pressure-drop reduction and fouling resistance. The design comparison indicated that wing-like designs had minimal impact on membrane permeability, while COM-M1 and COM-M2 increased reverse osmosis (RO) membrane flux by up to 19.8%. Fouling experiments further demonstrated that nanofiltration (NF) membranes, with looser separation layers, could be more prone to concentration polarization thanRO membranes, particularly after humic acid fouling. All these findings offer a basis for feed spacer optimization and provide critical insights for matching the spacer design with different membrane processes.
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高影响因子
FOOD CHEMISTRY
·
2026
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IF 10.4
Ru Jia,Sha Yan,Xuezhe Huang,Zongyan Cui,Xiaofeng Xue
Honey is primarily composed of sugars, with their profiles governing its quality. Twelve types of sugars in 784 honey samples, including 20 types of Chinese monofloral honeys, were accurately quantified by ultra-high performance liquid chromatography coupled with evaporative light scattering detection. Turanose (1.48 ± 0.63 g/100 g) was the most abundant oligosaccharide, followed by maltose (1.33 ± 0.43 g/100 g), and erlose (0.96 ± 0.80 g/100 g) was the predominant trisaccharide. Using unsupervised clustering, these honey samples were segmented into three clusters: high-oligosaccharide (e.g., jujube and fennel honey), high-glucose and low-oligosaccharide (e.g., rape, lychee and rubber honeys) and moderate-oligosaccharide clusters. The correlation analysis revealed strong correlations among turanose, maltulose, kojibiose and isomaltose, suggesting co-transformation among these sugars during honey ripening process and guiding future research directions. This study elucidated the sugar profile characteristics of Chinese honey, laying the foundation for enhancing its quality.
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高影响因子
危险品化学品经营许可证（带存储）