CHEMICAL ENGINEERING JOURNAL
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2023
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IF 15.1
Haixiang Lv,Xuchao Li,Jiaqing Li,Chang Yu,Qinghong Zeng,Guogui Ning,Hu Wan,Jianhong Li,Kangsheng Ma,Shun He
The cotton aphid Aphis gossypii Glover (Homoptera: Aphididae) is a notorious agricultural pest that has evolved high levels of resistance to various insecticides. Innovative RNA insecticides, as potential green plant protection products, are anticipated to alleviate the significant issue of pest resistance. Herein, a rough-surface hollow mesoporous silica (RHMS)-based gene/insecticide delivery system was constructed for the co-delivery of CYP6CY13 double-stranded RNA (dsRNA) and the chemical insecticide imidacloprid for the effective control of A. gossypii . The hollow mesoporous structure of RHMS facilitates insecticide loading and further combines with dsRNA through electrostatic interactions to form nanoscale complexes, while protecting the dsRNA from complete degradation by nucleases. The screened ds CYP6CY13 fragment was successfully expressed in the L4440-HT1115 (DE3) RNaseIII - system and inhibiting CYP6CY13 expression by RNA interference (RNAi) significantly increased the susceptibility of A. gossypii adults to imidacloprid. The toxicity of the RHMS/IMI/ds CYP6CY13 complex was significantly higher (1.95-fold) than that of imidacloprid. The results of the pot test showed that the effectiveness of the RHMS/IMI/ds CYP6CY13 complex against A. gossypii improved by 19.95 % in 5 days. Therefore, this nanoparticle-mediated gene/insecticide co-delivery system is expected to be an effective tool for pest control.
研究领域:
农业昆虫学
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分子生物学
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植物保护
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产品关联
DOI
高影响因子
Horticulturae
·
2025
·Vol. 11
·期 5
·490
·
IF 3
Jiawei Ma,Ye Tian,Xuli Liu,Shengyou Fang,Chong Sun,Junliang Yin,Yongxing Zhu,Yiqing Liu
Aphis gossypii Glover (Homoptera: Aphidinae), a major pest of Chinese pepper (Zanthoxylum bungeanum Maxim), causes significant agricultural damage. Ginger (Zingiber officinale Roscoe) has shown potential as a source for developing botanical pesticides due to its strong bacteriostatic and insecticidal properties; however, the underlying mechanisms remain poorly understood. This study evaluated the repellent activity of ginger shoot extract (GSE) across four solvent phases—petroleum ether, trichloromethane, ethyl acetate, and methanol—against A. gossypii. The results demonstrated that GSE exhibited significant repellent effects, with the methanol phase showing the most pronounced activity. Twelve fractions were chromatographically separated from the methanol phase, and electroantennography (EAG) analysis revealed that fraction 4 induced strong EAG responses in both winged and wingless aphids. Further identification of active compounds in fraction 4 by gas chromatography–mass spectrometry (GC–MS) indicated the presence of terpenes, aromatics, alkanes, esters, and phenols as major constituents. Subsequent EAG analysis identified several key compounds—octahydro-pentalene (C1), (Z)-cyclooctene (C2), dimethylstyrene (C3), tetramethyl-heptadecane (C5), tetrahydro-naphthalene (C6), and heptacosane (C9)—as responsible for eliciting EAG responses in both aphid forms. Additionally, results from Y-tube olfactometer assays showed that (Z)-cyclooctene and heptacosane were significantly attractive, while octahydro-pentalene acted as a strong repellent to both winged and wingless aphids. These findings offer valuable insights for the development of synthetic attractants and repellents for A. gossypii and provide a theoretical foundation for utilizing ginger in the creation of botanical pesticides targeting this pest.
研究领域:
昆虫学
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植物化学
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农业害虫防治
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产品关联
DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2021
·Vol. 139
·期 14
·51895
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IF 3.125
Weihua Su,Yan Qin,Guihua Meng,Jianning Wu,Shengchao Yang,Lin Cui,Wenjuan Li,Zhiyong Liu,Xuhong Guo
Intelligent response carriers have been developed and widely used in agriculture to improve pesticide utilization. However, there are few tailored response carriers that are targeting the cotton aphid pest. Based on the temperature-dependent growth and reproduction of cotton aphids, we constructed a tailored temperature-responsive star-shaped polymer to achieve on-demand controlled release of imidacloprid (IMI) to more effectively kill cotton aphids. In this work, a series of star-shaped polymers based on β -cyclodextrin ( β -CD), 2-(2-methoxyethoxy)ethyl methacrylate (MEO 2 MA), and tert -butyl acrylate ( t BA) were synthesized via atom transfer radical polymerization (ATRP). Next, β -CD-PMEO 2 MA ( β -CD-P0) micelles were developed having different controlled release rates of IMI at different temperatures (14, 21, or 27°C) following the cotton aphid growth and reproduction cycle. At 21°C, the micelle underwent a phase transition from a solution state to a turbidity state. By inducing this structural change, release of IMI was increased to adapt to the reproduction of the cotton aphid population which is highest at 21–27°C. In vitro release experiments showed that 48% of IMI was released from β -CD-P0 after the phase transition temperature (PTT). An aqueous solution of the β -CD-P0 polymer micelle was stable at 4°C for at least 28 days. Therefore, this intelligent response release model has enormous potential as a novel carrier for various pesticides.
研究领域:
农业科学
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材料科学
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DOI
高影响因子
JOURNAL OF PEST SCIENCE
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2024
·Vol. 97
·期 4
·2019-2034
·
IF 4.3
Zhang Yongqian,Zhang Yue,Chen Aiqing,Huo Ran,Yan Hongyu,Zhang Zhiyong,Guo Honggang
Cotton aphids ( Aphis gossypii Glover) are important pests of cucurbit crops. Plant defenses at different cell layers, including the leaf surface, mesophyll cells, and phloem, are employed to defend aphids. Here, we assessed differences in aphid resistance among six watermelon varieties and elucidated the defense mechanisms underlying aphid-resistant/susceptible watermelon varieties. The population abundance, offspring number per female, and meantime of phloem-feeding (E2 phase) of aphids were the highest on XiNong (XN), followed by JingXin (JX), TianWang (TW), ZaoJia (ZJ), and MeiFuLai (MFL), and these parameters were the lowest on JinMeiDu (JMD). Further analyses showed that there was no correlation between the aphid resistance of six watermelon varieties and defenses at the leaf surface. For defenses at mesophyll cells, aphid infestation increased salicylic acid (SA) content at 48 h post-infestation (hpi) and ROS accumulation at 6 and 12 hpi in six watermelon varieties. For phloem defenses, aphid infestation increased callose content in JMD plants but decreased callose content in JX, TW, ZJ, MFL, and XN plants at 6, 12, 24, and 48 hpi. Moreover, callose deposition suppressed by 2-deoxy-d-glucose (2-DDG) neutralized the resistance of JMD plants and exacerbated the susceptibility of XN plants to aphids, which exhibited a higher population abundance and E2 phase time. Collectively, phloem defenses regulated by aphid-induced callose deposition were responsible for differences in aphid resistance among the watermelon varieties.
研究领域:
植物学
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昆虫学
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植物生理学
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DOI
高影响因子
ACS Applied Nano Materials
·
2023
·
IF 5.9
Weihua Su,Yan Qin,Jianning Wu,Guihua Meng,Shengchao Yang,Lin Cui,Zhiyong Liu,Xuhong Guo
After cotton is invaded by cotton aphids and other pests, the function of its defense system decreases, and it is more vulnerable to infection by bacteria. When plants are invaded by external bacteria, they quickly produce reactive oxygen species (ROS). In this paper, ROS-responsive pesticide release carrier materials are designed and prepared for solving bacterial infections in plants by ring-opening polymerization of ε-caprolactone (ε-CL) and β-cyclodextrin (β-CD) to obtain a hydrophobic guest (β-CD-PCL). Thioketal (TK) was modified by adamantane (Ad) and poly(ethylene glycol) monomethyl ether (mPEG5000-OH) to form a hydrophilic guest (mPEG5000-TK-Ad). β-CD-PCL and mPEG5000-TK-Ad interact with each other to form an amphiphilic polymer (mPEG5000-TK-Ad@β-CD-PCL), which is loaded with carbendazim (MBC) by self-assembly (MBC-mPEG5000-TK-Ad@β-CD-PCL). The polymer had ROS response characteristics, and the release rate of C increased from 30 to 57% after 120 h of ROS stimulation. The inhibition rate of Rhizoctonia S. was 92.23% when the effective concentration of MBC was 30 mg/L. The critical micelle concentration (CMC) of the polymer is 8.30 mg/L, and the micelle had storage stability for at least 14 days, showing good storage stability. Therefore, this ROS-responsive polymer carrier has great potential as a carrier for antimicrobial pesticides.
研究领域:
植物病理学
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材料科学
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农业科学
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产品关联
DOI
高影响因子
Frontiers in Plant Science
·
2025
·
IF 4.8
Zhipeng Bian,Ying Zhang,Bing Liu,Tao Zhang,Haibin Yuan,Yanhui Lu
Introduction: The cotton aphid (Aphis gossypii Glover) is a major global agricultural pest that damages cotton and numerous other economically significant crops through feeding and virus transmission. It possesses high adaptability and rapid reproduction rates, contributing to widespread resistance to chemical insecticides and thereby reducing the effectiveness of such control methods. It should be noted that plants have developed advanced chemical defense mechanisms over long periods of synergistic evolution, allowing them to synthesize volatile compounds. These compounds not only defend against herbivorous insects but also crucially reduce the development of pest resistance. Consequently, this study strives to explore plant-emitted volatiles as a potential eco-friendly alternative for aphid management.Methods: In this study, we first tested the behavioral responses of A. gossypii to the volatile blends of fifteen native plant species in Xinjiang, China, using a Y-tube olfactometer and cage experiments. We identified six out of fifteen plant species that were repellent to A. gossypii. We then collected the volatile compounds of repellent plants using a headspace collection method and used gas chromatography-mass spectrometry (GC-MS) to identify the key components. The antennal responses of winged A. gossypii to the compounds were evaluated with an antennal potential test. Finally, further testing using a Y-tube olfactometer and Petri dish experiments.Results: We identified six out of fifteen plant species (i.e. Anethum graveolens L., Juglans regia L., Rhaponticum repens L., Karelinia caspia Pall., Launaea polydichotoma Ostenf., and Brassica rapa L.) that were repellent to A. gossypii. We collected the volatile compounds of these six repellent plants and identified thirty-one key components. Our electroantennogram (EAG) tests revealed that sixteen of the thirty-one compounds caused significant antennal responses in winged A. gossypii. Further testing using a Y-tube olfactometer and Petri dish experiments confirmed fourteen compounds that repelled intact, winged cotton aphids.Discussion: Our study report that the volatiles of four plant species – R. repens, K.caspia, L. polydichotoma, and B. rapa – present a significant repellent effect on winged cotton aphids, suggesting that these compounds might be useful for eco-friendly cotton aphid pest management. These results provide essential theoretical foundations and practical knowledge for the application of plantderived repellent volatiles.
研究领域:
昆虫学
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植物化学
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农业生态学
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DOI
PubMed
高影响因子
CHEMOSPHERE
·
2024
·
IF 8.1
Xiaohui Yan,Yongpan Shan,Yajie Ma,Yanqin Wang,Changcai Wu,Xiangliang Ren,Xianpeng Song,Dan Wang,Hongyan Hu,Xiaoyan Ma,Yan Ma
Nanotechnology aligns with the requirements of sustainable development of agriculture, and nano-pesticides offer a promising approach to controlling agricultural pests and increasing productivity. Non-target predators also play a crucial role in pest controls and enhancing the efficacy of pesticide on target organisms. Reducing the toxicity of pesticides to non-target organisms is key to of coordinating chemical control and biological control methods. Therefore, it is essential to assess the toxicity of nano-pesticides on non-target predators. In this study, a carbon dots-doped mesoporous silica nano-delivery system (Thi@CD-MSN) was successfully developed using CD-MSN as carrier material and thiacloprid (Thi) as a model pesticide. The results demonstrated that the synthesized Thi@CD-MSN exhibited a relatively high loading efficiency (33.58%). Laboratory bioassay experiments revealed that Thi@CD-MSN demonstrated effective insecticidal activity (LC 50 = 21.67 mg/L) in controlling Aphis gossypii Glover. Besides, the acute toxicity of Thi@CD-MSN on Chrysoperla pallens larvae was significantly lower than that of Thi, as was its toxicity to 4T1 cells. These findings suggest that CD-MSN can serve as an ecological safety carrier for pesticide delivery, improving the effective utilization of Thi while reducing the risks to non-target predators. These results are essential for comprehending the effects of nano-pesticides on non-target predators, providing informative data for implementing biological and chemical control strategies. It strengthens the safety evaluation of nano-pesticides.
研究领域:
农业科学
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纳米技术
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害虫管理
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毒理学
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产品关联
DOI
PubMed
高影响因子
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