Food Bioscience
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2026
·
IF 6.2
Lihua Fan,Mingying Hu,Zongyuan Ou,Fan Li,Yiheng Shi,Di Wu,Yongning Wu,Guoliang Li
Sensitive, rapid, and reliable detection of aflatoxin B1 (AFB1) in food is of great importance for ensuring food safety. In this study, a dual-mode paper-based biosensing platform was developed by integrating the CRISPR/Cas12a nucleic acid cleavage system with a nanozyme-responsive DNA hydrogel for highly sensitive and portable detection of AFB1. Upon the presence of the target, AFB1 binds specifically to the aptamer, thereby activating the CRISPR/Cas12a system and subsequently inducing the degradation of the hydrogel network, which leads to the release of the encapsulated Au@Pt nanozyme. The released nanozyme catalyzes the oxidation of TMB in the presence of H 2 O 2 to generate a blue-colored product, while simultaneously producing a quantifiable diffusion signal on the paper-based substrate, enabling dual-mode colorimetric and distance-based detection. The detection limit of naked-eye detection is 0.1 ng mL −1 , and it is 0.075 ng mL −1 in the distance detection mode. The proposed method can complete the signal output within 30 min and exhibits the advantages of operational simplicity, rapid response, and high sensitivity. In practical peanut samples, satisfactory recoveries and good stability were achieved, demonstrating its potential as a promising strategy for on-site detection of trace mycotoxins in food.
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MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 8
·584
·
IF 5.4
Zheng Xiaoqi,Li Yuan,Long Jie,Zhang Tianyu,Zhou Binbin,Li Shan,Zhang Li
A composite modified electrochemical sensor was developed based on molecular imprinting technology for zearalenone (ZLE) detection. The sensor was fabricated on a glassy carbon electrode (GCE) by first electrochemically reducing and depositing graphene oxide nanoribbons (rGNR) to form a highly conductive three-dimensional network. Subsequently, palladium-metallene (Pd-Me) was coated to enhance the electrochemical response. Finally, acrylamide molecularly imprinted polymer (AMIP) was polymerized in situ under infrared light to impart specific recognition capability. Performance tests demonstrated that the fabricated sensor (AMIP/Pd-Me/rGNR/GCE) exhibited a linear response to ZLE in the range of 1.0 − 1000 ng/mL, with a detection limit of 0.33 ng/mL (S/ N = 3). The sensor also showed good selectivity, repeatability (signal attenuation ≤ 5.63% after five consecutive detections), reproducibility (relative standard deviation, RSD = 2.62%), and storage stability (96.85% signal retention after two weeks at 4 °C). When applied to actual samples (grain flour, soybean flour, and corn flour), the spiked recoveries ranged from 97.26% to 104.09%, with RSD below 4.58%. These results confirm that the sensor can achieve precise quantitative detection of ZLE in complex food matrices. The sensor preparation process, which combines electro-polymerization and coating, is characterized by simple operation, strong film formation, low cost, and ease of miniaturization, offering a promising new approach for the rapid determination of mycotoxins in food safety. Graphical
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MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 7
·503
·
IF 5.4
Xiao Yue,Wen Xuefei,Zhang Xingzhong,Chen Hongrui,Tang Jie,Chi Hai,Li Liman,Thanapop Soteyome
To enable sensitive quantitative detection of zearalenone (ZEN) in cereal matrices, this study develops a molecularly imprinted electrochemical sensor. A three-dimensional flower-shaped carbon composite material (CoNiZn@C), synthesized from a ternary CoNiZn metal–organic framework, was utilized as a highly conductive substrate featuring excellent electrical conductivity and abundant binding sites. Density functional theory calculations were conducted to elucidate the intermolecular interactions and cooperative recognition mechanisms between zearalenone and the selected monomers. Experimentally, the AuNPs-based thioaniline units (PATP@AuNPs) and pyrrole were employed as bifunctional monomers. Specifically, PATP@AuNPs can be stably immobilized on the substrate surface to enhance interfacial electron transfer efficiency, while simultaneous in situ copolymerization of the two monomers generates numerous non-covalent binding cavities capable of specifically recognizing ZEN. Under optimized experimental conditions, the sensor exhibited a linear detection range from 10 nM to 1000 µM and a detection limit as low as 1.6 nM. This detection approach is straightforward, cost-effective, and demonstrates excellent specificity and sensitivity, indicating substantial potential for rapid screening and quantitative analysis of mycotoxins in agricultural products. Graphical
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Wenyan Yan,Shuyue Li,Baoqing Bai,Ying Zhang,Jinhua Zhang,Tao Bo,Qinghua Wang,Ying Gu,Yukun Yang,Shuo Wang
A MIP-modified MOF/COF hybrid nanoplatform for PEC sensing, adsorption, and photocatalytic degradation was developed. • The MOF/COF hybrid enhanced photocurrent through improved light absorption and charge separation. • The MIP layer provided high selectivity. • The platform enabled ultrasensitive PAT detection and efficient adsorption-photocatalytic degradation. Rational design of photoactive materials is essential for the sensitive detection and efficient removal of hazardous contaminants. Herein, a visible-light-responsive covalent organic framework/metal-organic framework (COF/MOF) hybrid with uniform COF layers grown in-situ on the MOF surface, followed by the introduction of a molecularly imprinted polymer (MIP) layer via a sol-gel process, was constructed for the PEC sensing, adsorption, and photocatalytic degradation of patulin. The hybrid architecture enhanced photoelectrochemical performance by facilitating charge separation and improving light harvesting. The resulting PEC sensor exhibited a wide linear range (0.1 nM-1 mM) and a low detection limit (0.055 nM), along with good reproducibility and stability in real samples. Moreover, the MIP/MOF/COF hybrid enabled efficient adsorption and photocatalytic degradation of patulin, driven by photogenerated holes and superoxide radicals. This work provides a promising strategy for the efficient and highly sensitive detection and removal of contaminants. Download: Download high-res image (236KB) Download: Download full-size image
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SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Tao Liao,Dan Hou,Hao Huang,María Belén Camarada,Xiaoning Liao
Herein, a novel ratiometric molecularly imprinted electrochemical sensor (MIP-RECS) based on Ag nanoparticles (AgNPs) and ionic liquid (IL) co-modified Ti 2 NTx (AgNPs@Ti 2 NT x @IL) was developed for the determination of patulin (PAT). Firstly, the AgNPs@Ti 2 NT x @IL nanocomposite, was prepared by an in situ reduction and self-assembled method, and then, was cast on the glassy carbon electrode (GCE) to build an electrochemical interface with excellent conductivity and high surface area. After that, the MIP was deposited on the nanocomposite modified GCE by the electro-polymerization technique, with thionine (Thi) and PAT as functional monomer and template molecules. In the sensing platform, the oxidation peaks generated by Thi and AgNPs were used as reference and probe signal, respectively. The introduction of reference effectively eliminates the influence of the matrix effect. At the same time, the oxidation signal of the Ag probe is greatly improved by the high conductivity and large surface area of the AgNPs@Ti 2 NT x @IL nanocomposite. Therefore, the MIP-RECS presented a wide linear range, a very low low limit of detection (LOD), together with high selectivity and excellent repeatability. Additionally, the linearity ranges and LODs of the sensor can be controlled by the amount of Ag, highlighting its robustness in different types of real samples. Furthermore, this sensing model can be used to sensitively detect other residues with poor redox activity, such as imidacloprid and catechol, highlighting its versatility and universality.
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Processes
·
2023
·Vol. 11
·期 3
·864
·
IF 3.5
Sai Zhang,Yahui Wang,Qinglin Sheng,Tianli Yue
Ochratoxin A(OTA) is positively correlated with an increased risk of developing cancer in nephrotoxic and hepatotoxic patients. Therefore, it is of great significance for the highly sensitive, highly selective, and timely detection of OTA. We described here an electrochemical aptasensor for OTA analysis, which took advantage of the favorable properties of gold nanoparticles (AuNPs) functionalized zinc oxide (ZnO) composites and the intercalative binding between methylene blue (MB) and nucleic acid. There were two label-free aptamers: one to capture OTA and another serving as complementary DNA (cDNA), enabling connection to the ZnO-Au composite’s immobilized electrode. Once OTA was present, the aptamer could capture OTA and detach from the electrode interface, thus, preventing MB from accessing electrode surface for efficient electron transfer; a decreased peak current was monitored by differential pulse voltammetry. The aptasensor presented nice analytical performance for OTA detection in the range of 0.1–30,000 pg·mL−1, with a detection limit of 0.05 pg·mL−1. Moreover, the developed biosensor could be applied to actual sample (wine and beer) analysis.Keywords:food safety;electrochemical aptasensor;intercalative binding;zinc oxide-gold composites;Ochratoxin A
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Analytical Methods
·
2023
·Vol. 15
·期 19
·2376-2381
·
IF 3.1
Lei Jiang,Lin Yuan,Shan Gao,Yingying Xiang,Fei Song,Wensi Ma,Jing Wan,Xiuling Ji,Yujiao Tu
Pesticides are enormously important to industry requirements and in agriculture for improving yields. Parathion is widely used to control pests in vegetables, fruits, and flowers. However, excessive use of parathion poses a threat to food safety, the environment, and human health. Due to low cost, ease to use, and high selectivity and sensitivity, a fluorescent nanoprobe is a candidate for parathion detection. Blue fluorescent carbon dots were prepared by a hydrothermal method with rutinum and o-phenylenediamine as precursors. The Rut-CDs were purified by using dialysis, thin layer chromatography, and a chromatographic column. Excellent linear ranges of 0–7.5 μg L−1 and 12.5–62.5 μg L−1 parathion were obtained with a low detection limit of 0.11 ng mL−1. In addition, the quenching mechanism of parathion to the fluorescence of Rut-CDs was illuminated. Furthermore, the nanoprobe was efficiently applied to determine the parathion content in Chinese cabbage, cantaloupe, and cowpea samples. It shows great potential to detect parathion.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Chen Hu,Caiping Huang,Bangzhu Peng
Patulin poses a potential risk to human health, and current methods for removing it have certain limits. Thus, the effective and secure technique for degrading patulin in juice is critical. In this study, a nitrogen-doped chitosan-TiO 2 nanocomposite (N-TiO 2 Nps) as a photocatalyst was employed to decompose patulin. Under the action of the photocatalyst, 500 μg/L patulin was completely degraded within 1 h in simulated juice. Quenching experiments identified superoxide and hydroxyl radicals as the dominant species responsible for patulin degradation. On the bases of liquid chromatography-mass spectrometry (LC-MS) and density functional theory (DFT) calculation, the reaction sites in patulin were predicted and a possible photodegradation pathway was proposed. The findings not only elucidated a new method for removing patulin but also provided a theoretical basis for scrutinizing the degradation mechanism of mycotoxins.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Dongyan Tian,Jie Wang,Qiandong Zhuang,Songmei Wu,Yu Yu,Kejian Ding
Based on graphite-like carbon nitride (g-CN) nanocomposites coupled with aptamer, a regenerable electrochemiluminescence (ECL) biosensor is developed for the quantitative detection of aflatoxin B 1 (AFB 1 ). In the existence of AFB 1 , the structure of the aptamer changed into a loop, and the original ECL intensity was reduced owing to the enhancement of luminescence quenching between the ferrocene modified at the end of the aptamer and the luminescent substrate g-CN. Moreover, AFB 1 with oxidation state could also react with high energy state g-CN, leading to further reduction of the electrochemiluminescence signal. At optimum conditions, ECL intensity was decreased in linearity with an AFB 1 concentration range from 0.005 ng/mL to 10 ng/mL, and the minimum detectable concentration was down to 0.005 ng/mL, which realized trace detection demand with high sensitivity. It was selective for AFB 1 and its performance had been verified on rice samples, which indicated a promising applying prospect of non-enzymatic electrochemiluminescence AFB 1 detection.
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FOOD AND CHEMICAL TOXICOLOGY
·
2022
·
IF 5.572
Hui Chen,Lixing Cao,Kai Han,Han Zhang,Jinling Cui,Xuan Ma,Shuang Zhao,Chong Zhao,Shutao Yin,Lihong Fan,Hongbo Hu
Patulin (PAT) is a common food-borne mycotoxin with diverse toxic effects including nephrotoxicity . The induction of oxidative stress is suggested to be a key mechanism contributed to toxicities of PAT. Reduced glutathione (GSH), a sulfhydryl-containing tripeptide , is a key reason for PAT-mediated oxidative stress. Cystine/glutamate antiporter (system x c − )-mediated cystine uptake plays a critical role in maintaining redox balance via promoting GSH biosynthesis. In this study, we addressed if GSH reduction by PAT was associated with inhibition of system x c − -mediated GSH biosynthesis. Results showed that PAT significantly decreased activity of SLC7A11, a core subunit of system x c − , through activating AMPK-mediated formation of beclin1-SLC7A11 complex. Furthermore, PAT promoted ferroptosis induced by a known ferroptosis inducer RSL3 in normal renal cells, and exacerbated folic acid-induced nephrotoxicity in a mouse model of acute kidney injury . The findings of the present study provide new insights into PAT-induced kidney toxicity, and implicate that patients with ferroptosis-associated diseases maybe more susceptible to PAT.
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Toxins
·
2022
·Vol. 14
·期 2
·137
·
IF 5.075
Lu Yang,Jin Wang,Huan Lv,Xue-Meng Ji,Jing-Min Liu,Shuo Wang
Aflatoxin (AFT) contamination, commonly in foods and grains with extremely low content while high toxicity, has caused serious economic and health problems worldwide. Now researchers are making an effort to develop nanomaterials with remarkable adsorption capacity for the identification, determination and regulation of AFT. Herein, we constructed a novel hollow-structured microporous organic networks (HMONs) material. On the basis of Fe3O4@MOF@MON, hydrofluoric acid (HF) was introduced to remove the transferable metal organic framework (MOF) to give hollow MON structures. Compared to the original Fe3O4@MOF@MON, HMON showed improved surface area and typical hollow cavities, thus increasing the adsorption capacity. More importantly, AFT is a hydrophobic substance, and our constructed HMON had a higher water contact angle, greatly enhancing the adsorption affinity. From that, the solid phase extraction (SPE-HPLC) method developed based on HMONs was applied to analyze four kinds of actual samples, with satisfied recoveries of 85–98%. This work provided a specific and sensitive method for the identification and determination of AFT in the food matrix and demonstrated the great potential of HMONs in the field of the identification and control of mycotoxins.Keywords:food-safety inspection;solid-phase extraction;aflatoxins;HMONs;adsorbentsKey Contribution:Compared to the original Fe3O4@MOF@MON, the large specific surface and hydrophobicity of both inner and outer bilayers enable HMONs to retrieve aflatoxin from different grain extracts ultra-quickly and efficiently. The HMONs-SPE-HPLC was applied to analyze four kinds of actual samples, with satisfied recoveries.
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CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Xiaobing Yang,Junjie Pan,Jiapeng Hu,Shengyun Zhao,Kejun Cheng
During the planting, storage, or feeding stages, foodstuffs can be easily polluted by mycotoxins such as aflatoxin B 1 , patulin and zearalenone, which are produced by pathogenic microorganisms. Therefore, the removal of these mycotoxins constitutes an active area of research. Herein, we used an Fe/Zn metal–organic framework (MOF) as a precursor to fabricate a novel La–ZnFe 2 O 4 @Fe 3 O 4 @carbon magnetic hybrid composite via a simple absorption–pyrolysis method and explored its photocatalytic performance in the degradation of aflatoxin B 1 , patulin, and zearalenone. La–ZnFe 2 O 4 @Fe 3 O 4 @carbon can effectively degrade 98.37% of aflatoxin B 1 , outperforming Fe 3 O 4 @carbon (69.57%) and ZnFe 2 O 4 @Fe 3 O 4 @carbon (91.39%). Moreover, La–ZnFe 2 O 4 @Fe 3 O 4 @carbon exhibits superior stability and excellent photocatalytic activity for the degradation of patulin (97.35%) and zearalenone (98.52%). Transient photocurrent response measurements reveal that the La doping and the combination with ZnFe 2 O 4 highly promote the separation of photoinduced electron–hole pairs on Fe 3 O 4 @carbon. An electron spin resonance analysis suggests that · OH and · O 2 − are the main active radicals during the photodegradation process. Thus, a plausible photodegradation pathway for the degradation of mycotoxins produced by pathogenic microorganisms is proposed.
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