ACS Applied Nano Materials
·
2024
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IF 5.3
Yu Tian,Lili Cong,Hongda Sun,Wenli Wei,Fengxia Wu,Fenghua Li,Guobao Xu,Wenxin Niu
Determining whether plants are under stress before obvious disease symptoms is crucial for precision agriculture. Intercellular hydrogen peroxide (H2O2) in plants can be used to indicate stress. However, most current H2O2 detection methods are destructive or limited by chlorophyllous autofluorescence. Herein, we constructed an in vivo surface-enhanced Raman scattering (SERS) sensor for nondestructive, in situ, and highly sensitive detection of H2O2 in plant leaves. The sensor with the designed size can enable nondestructive and in situ H2O2 detection without fluorescence interference. In addition, Ag decahedral nanoparticles with sharp edges and tips enhance the SERS effect, and the lowest detection concentration of H2O2 can reach 10 nM in vitro. The utility of the SERS sensor is demonstrated by Oxalis corniculata under wound, heat, and cold stresses. This SERS sensor can provide warnings before plants show visible signs of damage, thereby improving crop yields and potentially becoming an essential tool for precision agriculture.
研究领域:
植物学
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农业科学
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
BMC MICROBIOLOGY
·
2025
·Vol. 25
·期 1
·1-12
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IF 4.2
Yang Manli,Zuo Shanningmei,Liu Ahui,Zhong Nana,Lu Xueping,Liu Xun,Jiang Xiasen,Hu Tao,Liu Yuntong,Ren Xiaogang,Zhou Kang,Wu Chuansheng
Background Bisphenol A (BPA), a widespread environmental pollutant, has been extensively studied for its effects on bacteria and plant, but its impact on rhizosphere bacterial communities and plant root traits is less understood. At the same time, the role of bacteria in helping plants resist adversity is widely recognized, but the relationship between BPA-induced with rhizosphere bacterial changes and root development is still unclear. Therefore, this study investigated the effects of varying BPA concentrations (1.5, 17.2, and 50 mg/L) on soybean root traits and rhizosphere bacterial communities, as well as the relationship between them. Result The results revealed that BPA exposure significantly altered root traits, with root length, surface area, volume, and tip numbers being suppressed at 50 mg/L, while lower concentrations (1.5 and 17.2 mg/L) promoted root elongation and thickening. Bacterial community composition shifted notably, with Bacillota increasing and Pseudomonadota decreasing in relative abundance across all BPA treatments. Alpha diversity, measured by richness and Shannon_e indices, increased slightly at lower BPA concentrations, while beta diversity (Bray_Curtis and UniFrac) analysis showed significant differences, particularly at 50 mg/L. Community assembly processes (βNRI and βNTI) were dominated by deterministic mechanisms at lower BPA concentrations but shifted toward stochastic processes at 50 mg/L. Correlation analysis revealed significant relationships between bacterial community dynamics and root traits (Principal component PC1 and PC2), with alpha diversity indices influencing root traits represented by PC2 and beta diversity indices showing a negative correlation with PC1. Conclusions BPA exposure not only alters root morphology and bacterial community structure but also highlights the intricate interplay between rhizosphere bacteria and plant roots under BPA stress. This study contributes to the theoretical understanding of plant–microbe interactions in contaminated environments and may inform future research on microbial involvement in plant stress responses.
研究领域:
植物科学
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环境毒理学
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微生物生态学
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农业污染
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Small
·
2024
·
IF 13
Xinyue Wu,Yuxiang Pan,Xunjia Li,Yuzhou Shao,Bo Peng,Chao Zhang,Chi Zhang,Shiyun Yao,Jianfeng Ping,Yibin Ying
The rapidly changing climate is exacerbating the environmental stress that negatively impacts crop health and yield. Timely sensing of plant response to stress is beneficial to timely adjust planting conditions, promoting the healthy growth of plants, and improving plant productivity. Hydrogen peroxide (H 2 O 2 ) is an important molecule of signal transduction in plants. However, the common methods for detecting H 2 O 2 in plants are associated with certain drawbacks, such as long extraction time, cumbersome steps, dependence on large instruments, and difficulty in realizing in-field sensing. Therefore, it is urgent to establish more efficient detection methods to realize the rapid detection of H 2 O 2 content in plants. In this research, poly (methyl vinyl ether- alt -maleic acid) (PMVE/MA) hydrogel microneedle (MN) patch for rapid extraction of leaf sap are prepared, and the extraction mechanism of PEG-crosslinked PMVE/MA hydrogel MN patch is studied. A method of rapid detection of H 2 O 2 content in plants based on MN patch with optical detection technology is constructed. The hydrogel MN patch can be used for timely H 2 O 2 analysis. This application enables new opportunities in plant engineering, and can be extended to the safety and health monitoring of other plants and animals.
研究领域:
农业科学
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生物技术
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植物生理学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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