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JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE · 2018 ·Vol. 55 ·期 3 ·964-976 · IF 1.797

Improving the quality of matured coconut (Cocos nucifera Linn.) water by low alcoholic fermentation with Saccharomyces cerevisiae: antioxidant and volatile profiles

Zhang Guanfei,Chen Wenxue,Chen Weijun,Chen Haiming

Matured coconut water (MCW) is a by-product in the coconut milk industry that is usually discarded due to its unpleasant flavor. In this study, low-alcohol coconut water (LACW) was fermented with Saccharomyces cerevisiae to improve the quality of MCW. Volatile components and nonvolatile flavor-related elements were estimated to compare the qualities of the MCW and LACW. Besides measuring the kinetic changes, the levels of fructose, glucose, sucrose and ethanol contents were also determined. The results of the organic acid assays showed that tartaric, pyruvic and succinic acids were the primary organic acids present in LACW and increased significantly with fermentation. The resulting volatile composition assay indicated that esters, alcohols and fatty acids were significantly influenced by fermentation and yeast strains. Moreover, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), cupric ion reducing antioxidant capacity and ferric reducing antioxidant power values increased significantly throughout the process, correlating with the enhancement of total phenolic content.

研究领域: 食品科学 · 生物技术 · 化学
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Foods · 2020 ·Vol. 9 ·期 1 ·46 · IF 4.092

Metabolomics Analysis of the Deterioration Mechanism and Storage Time Limit of Tender Coconut Water during Storage

Yunwu Zhang,Wenxue Chen,Haiming Chen,Qiuping Zhong,Yonghuan Yun,Weijun Chen

Tender coconut water tastes sweet and is enjoyed by consumers, but its commercial development is restricted by an extremely short shelf life, which cannot be explained by existing research. UPLC-MS/MS-based metabolomics methods were used to identify and statistically analyze metabolites in coconut water under refrigerated storage. A multivariate statistical analysis method was used to analyze the UPLC-MS/MS datasets from 35 tender coconut water samples stored for 0–6 weeks. In addition, we identified other differentially expressed metabolites by selectingp-values and fold changes. Hierarchical cluster analysis and association analysis were performed with the differentially expressed metabolites. Metabolic pathways were analyzed using the KEGG database and the MetPA module of MetaboAnalyst. A total of 72 differentially expressed metabolites were identified in all groups. The OPLS-DA score chart showed that all samples were well grouped. Thirty-one metabolic pathways were enriched in the week 0–1 samples. The results showed that after a tender coconut is peeled, the maximum storage time at 4 °C is 1 week. Analysis of metabolic pathways related to coconut water storage using the KEGG and MetPA databases revealed that amino acid metabolism is one of the main causes of coconut water quality deterioration.

研究领域: 食品科学 · 代谢组学 · 植物学
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Journal of Cleaner Production · 2024 · IF 9.7

Eco-friendly production of leather-like material from bacterial cellulose and waste resources

Fan Yang,Zexuan Zhang,Jiugang Yuan,Jin Xu,Qiao Ji,Yutong Bai

In recent years, resource optimization has been recognized as a top priority among many challenges. Studies have indicated that valuable products can be created by combining natural or discarded resources with cutting-edge biosynthesis techniques. This approach is considered foundational for establishing a “zero-waste” socio-economic structure. This research focuses on optimizing resources and minimizing environmental impacts, especially within the manufacturing and fashion sectors. An innovative leather-like material named BC-BioLeath (Bacterial Cellulose-Based Bio Leather) has been introduced. BC was produced by the fermentation of coconut water, a byproduct of the food industry. Traditional leather-like processing, including tanning, was applied to BC using food waste such as oxidized edible oil and coffee grounds, along with timber industry by-products like vegetable tannins. Studies involving SEM observation, mechanical, style, and dyeing performance testing revealed that the product, BC-BioLeath, exhibited superior mechanical properties. For instance, BC crosslinked with oxidized edible oil showed a tensile strength of 82.14 MPa, surpassing traditional cowhide used in shoe uppers by 2.08 times. When plasticized with glycerol, the tensile strength reached 56.19 MPa, with breaking elongation of 36%. Beyond its mechanical prowess, the product also achieved economic and environmental benefits through waste repurposing, contributing to a natural energy cycle. BC-BioLeath was identified as a more humane and eco-friendly leather alternative. This method showcased not just efficient waste repurposing but also introduced a novel, microbe-focused sustainable production approach. Such a model offered a genuine circular economy solution for high-polluting sectors, such as the leather fashion industry. A cost analysis showed that BC-BioLeath's production cost of $97.92/m 2 (excluding labor and operating costs: $7.52/m 2 ) was within the commercial leather range of $39.7 to $220.8/m 2 . This indicated its sustainability as a competitive alternative.

CAS / MDL: 56-81-5 57-13-6 57-50-1
研究领域: 材料科学 · 环境科学 · 生物技术
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Food Frontiers · 2025 ·Vol. 6 ·期 3 ·1409-1422 · IF 6.9

Effects of Cold Plasma and Pasteurization on Flavor and Primary Metabolites of Aromatic Coconut Water Based on Flavoromics and Metabolomics

Lilan Xu,Jing Zhang,Xincheng Zhang,Meizhen Xie,Weimin Zhang,Jiamei Wang,Lipin Chen,Yong-Huan Yun

Aromatic coconut water (ACW) is a healthy drink with a unique aroma and high nutritional value. To better preserve ACW, exploring an efficient sterilization technique with good sterilization effects and fragrance retention effects is necessary. Pasteurization (PS) and cold plasma (CP) have been shown to be good sterilization techniques for effectively killing microorganisms in coconut water. However, there are few reports on the effects of PS and CP on the metabolites and flavor quality of ACW. This study systematically investigated the effects of CP and PS treatments on flavor compounds and primary metabolites of ACW using an integrated flavoromics and metabolomics approach. The results showed that compared with PS, CP had a less damaging effect on 2-AP and better retention of key flavors in fresh ACW. In addition, dimethyl trisulfide was produced in pasteurized ACW and became the key active flavor compound, promoting off-flavor formation. The results of the metabolomics analysis revealed that CP affected mainly amino acids and lipids and that PS affected mainly amino acids in the ACW. The results of the correlation analysis revealed that the sterilization process may modulate or modify the flavor of ACW by affecting these metabolites. This study offers a theoretical foundation for optimizing sterilization methods and regulating flavor quality in ACW and supports the potential application of CP technology in ACW preservation.

CAS / MDL: 64-17-5 64-18-6
研究领域: 食品科学 · 营养学 · 生物学
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SEPARATION AND PURIFICATION TECHNOLOGY · 2024 · IF 8.1

Synthesis of sandwich structure forward osmosis membrane with calcium-carboxyl modified polyamide and tannic acid-Fe3+ interlayer and its application in coconut water concentration

Chengyan Gao,Mantong Zhao,Shiyu Zeng,Jianfei Pei,Xinwen Wang,Chuan Li,Weimin Zhang,Zhongyuan Liu

In this study, a sandwich structure thin-film composite forward osmosis (TFC-FO) membrane based on calcium-carboxyl interfacial polymerization was synthesized using tannic acid-Fe (TA-Fe) as the interlayer. Additionally, the concentration effect of Ca 2+ surface modification, TA-Fe intermediate layers, and their combination on coconut water concentration, membrane efficiency, anti-fouling ability, and quality of the concentrate was investigated. Compared to the TFC-Ca or TFC-TA-Fe membrane, the TFC-TA-Fe-Ca membrane significantly enhanced the concentration effect and reverse salt flux ( p  < 0.05) and exerted the lowest attenuation of permeability. Furthermore, the total soluble solids content increased from 5.13 °Brix to 13.17 °Brix after 8 h. The TFC-TA-Fe-Ca membrane showed a 61.20 % increase in its efficiency compared to the TFC membrane. The total reducing sugar, acids, and polyphenol contents increased by 1.72, 1.34, and 1.22 times, respectively. Overall, the sandwich structure FO membrane exhibited significant feasibility and potential for industrial application in fruit juice concentrate production.

研究领域: 食品科学 · 材料科学 · 生物工程
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JOURNAL OF CHROMATOGRAPHY A · 2009 · IF 3.756

Simultaneous determination of several phytohormones in natural coconut juice by hollow fiber-based liquid–liquid–liquid microextraction-high performance liquid chromatography

Yunli Wu,Bin Hu

A simple, selective, sensitive and inexpensive method of hollow fiber-based liquid–liquid–liquid microextraction (HF-LLLME) combined with high performance liquid chromatography (HPLC)-ultraviolet (UV) detection was developed for the determination of four acidic phytohormones (salicylic acid (SA), indole-3-acetic acid (IAA), (±) abscisic acid (ABA) and (±) jasmonic acid (JA)) in natural coconut juice. To the best of our knowledge, this is the first report on the use of liquid phase microextraction (LPME) as a sample pretreatment technique for the simultaneous analysis of several phytohormones. Using phenetole to fill the pores of hollow fiber as the organic phase, 0.1   mol   L −1 NaOH solution in the lumen of hollow fiber as the acceptor phase and 1   mol   L −1 HCl as the donor phase, a simultaneous preconcentration of four target phytohormones was realized. The acceptor phase was finally withdrawn into the microsyringe and directly injected into HPLC for the separation and quantification of the target phytohormones. The factors affecting the extraction efficiency of four phytohormones by HF-LLLME were optimized with orthogonal design experiment, and the data was analyzed by Statistical Product and Service Solutions (SPSS) software. Under the optimized conditions, the enrichment factors for SA, IAA, ABA and JA were 243, 215, 52 and 48, with the detection limits (S/N   =   3) of 4.6, 1.3, 0.9   ng   mL −1 and 8.8   μg   mL −1 , respectively. The relative standard deviations (RSDs, n   =   7) were 7.9, 4.9, 6.8% at 50   ng   mL −1 level for SA, IAA, ABA and 8.4% at 500   μg   mL −1 for JA, respectively. To evaluate the accuracy of the method, the developed method was applied for the simultaneous analysis of several phytohormones in five natural coconut juice samples, and the recoveries for the spiked samples were in the range of 88.3–119.1%.

CAS / MDL: 21293-29-8 69-72-7
研究领域: 分析化学 · 植物学
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Food Science and Human Wellness · 2024 · IF 5.6

Bio-screening and quantification of methyl paraben in vinegar and coconut juice separated by HPTLC

Yisheng Chen,Xingjun Xi

As a widely used food preservative, methyl paraben was experimentally evidenced with serious hormone-like adverse effects. Herein, a high performance thin-layer chromatography platformed bioluminescent bioautography and image analysis for the selective quantification and confirmation of methyl paraben was proposed and validated in vinegar and coconut juice. First, the detectability of the bioautography to the analyte on different layer materials was estimated, revealing that normal silica gel was the best choice. After that, the liquid of sample extract and working solution were separated to overcome the background noises due to co-extracted matrices. The separation result was then coupled to the optimized bioautography, enabling instant and straightforward screening of the targeted compound. For accurate quantification, bioluminescent inhibition pattern caused by the analyte was processed by image analysis, giving useful sensitivity (LOD > 16 mg/kg), precision (RSD < 10.1 %) and accuracy (spike-recovery rate 76.9 %–112.2 %). Finally, the suspected result was confirmed by determining its MS fingerprint, further strengthening the reliability of screening.

CAS / MDL: 7128-64-5
研究领域: 食品科学 · 分析化学 · 毒理学
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ANALYTICAL SCIENCES · 2014 ·Vol. 30 ·期 4 ·489-494 · IF 1.403

Interference-free Determination of Indole-3-Acetic Acid in Two Real Systems Using Second-order Calibration Method Coupled with Excitation-emission Matrix Fluorescence

Xiu-Fang YAN,Hai-Long WU,Xiang-Dong QING,Yan-Mei SUN,Ru-Qin YU

In this work, a simple and practicable method that combines excitation-emission matrix (EEMs) fluorescence with a second-order calibration method based on parallel factor analysis-alternative least-squares (PARAFAC-ALS) algorithm was developed for the direct interference-free determination of indole-3-acetic acid (IAA) in two real systems, coconut water (CW) and coconut milk (CM). Although the excitation and emission profiles of IAA heavily overlapped with that of unknown interferents in the complex real systems, the actual contents and satisfactory recoveries were still obtained successfully. The contents of IAA in CW and CM were 10.8 ± 0.3 and 4.9 ± 0.2 μg mL−1, respectively, which were consistent with those reported by LC-MS/MS assays in the reference material. The average spike recoveries of IAA in the validation set based on CW and CM were 102.1 ± 3.2 and 98.0 ± 1.9%, respectively. In addition, routine experiments were performed for establishing the validity of the assay to internationally accepted criteria.

研究领域: 植物学 · 分析化学
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