COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2024
·
IF 4.9
Huanan Guan,Yanyu Chen,Yue Xue
The self-assembly approach has successfully been used to generate Fe 3 O 4 @Pt, a material that can mimic peroxidase . The material was created by modifying the surface of Fe 3 O 4 nanoparticles (Fe 3 O 4 NPs) with -NH 2 functional groups using 3-Aminopropyltriethoxysilane (APTES), and then using the coordination between platinum ions and -NH 2 on the surface of magnetic particles to reduce platinum ions to the surface of magnetic particles under ultrasonic conditions to produce Fe 3 O 4 @Pt composite nanoparticles . The Fe 3 O 4 @Pt core-shell composite material not only has the inert metal Pt to protect Fe 3 O 4 to improve the stability of Fe 3 O 4 in the biological matrix, but also has the high catalytic activity of Fe 3 O 4 and Pt. The catalysts were characterized, and the findings revealed that they were non-homogeneous Fenton catalysts with high dispersion, uniform particle size, and a large specific surface area . Since a large number of free radicals generated during the catalysis of Fe 3 O 4 @Pt mimic enzyme can trigger the Fenton reaction, tetracycline (TC) and oxytetracycline (OTC) were chosen as target substances, and a system for the combined degradation of tetracyclines pollutants by mimic enzyme and Fenton reaction was established, and the catalytic mechanism of this system has been investigated. The findings indicate that by using a concentration of 0.25 mol/L of H 2 O 2 , a catalyst amount of 1.5 g/L, a reaction time of 50 min, and a reaction temperature of 50°C, the degradation rate of TC and OTC can exceed 97 %, following first-order degradation kinetics. Under ideal conditions, over 95 % of each tetracyclines was degraded in the simulated food processing wastewater, effectively achieving tetracyclines degradation in the actual wastewater. In addition, the reusability of the recovered Fe 3 O 4 @Pt mimetic enzyme was investigated. The findings indicated that the Fe 3 O 4 @Pt mimetic enzyme has exceptional activity, stability, and reproducibility. The combination of the Fenton reaction with the Fe 3 O 4 @Pt mimetic enzyme can expedite the degradation of tetracyclines, offering a novel approach for eliminating recalcitrant contaminants in wastewater.
研究领域:
环境科学
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催化材料
·
生物化学
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 4
·1-8
·
IF 6.232
Zhang Qiao,Zhang Fengxia,Yu Lei,Kang Qi,Chen Yuqin,Shen Dazhong
A differential photoelectrochemical (PEC) method for glucose determination is reported using a nanocomposite with double mimic enzymes of glucose oxidase (GOx) and peroxidase. The nanocomposite was prepared by soaking zeolite imidazole framework-67 (ZIF-67) in 0.1 M NaOH solution at room temperature for 30 min, abbreviated as Co x O y H z @ZIF-67. The Michaelis–Menten constant of Co x O y H z @ZIF-67 to H 2 O 2 and glucose is 121 μM and 3.95 mM, respectively. Using the photoelectrode of Co x O y H z @ZIF-67/TiO 2 nanotubes (NTs), glucose was oxidized firstly by dissolved oxygen to generate H 2 O 2 under the catalysis of Co x O y H z film as the mimics of GOx. The product of H 2 O 2 enhanced the photocurrent of TiO 2 NTs under the catalysis of ZIF-67 as the mimics of peroxidase. The molecular sieve effect of ZIF-67 frameworks reduces the interferences from molecules with size larger than the apertures in ZIF-67. Under the excitation of a 150 W xenon lamp with full spectrum, the photocurrent was measured in a two-electrode system without external additional potential. By using the photocurrent difference between two photocells, i.e Co x O y H z @ZIF-67/TiO 2 NTs and Pt electrode, ZIF-67/TiO 2 NTs and Pt electrode, as the signal, the selectivity for glucose determination is improved further. The differential PEC method was applied to the determination of glucose with a linear range 0.1 μM~1 mM and a detection limit of 0.03 μM.
Graphical abstract
研究领域:
分析化学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2014
·
IF 5.825
Chenchen Ge,Quan Luo,Dou Wang,Shiming Zhao,Xiaoling Liang,Luxin Yu,Xuerong Xing,Lingwen Zeng
G-quadruplex-forming sequence can be formed through a copper(I) ion (Cu+)-catalyzed click chemistry between azide- and alkyne-modified short G-rich sequences in aqueous solution, eliminating immobilization and washing steps of conventional assays. The source for Cu+ was generated from the reduction of Cu2+ with the reductant of sodium ascorbate. In the presence of hemin and K+, the self-assembly of hemin/G-quadruplex structure has the activity of horseradish peroxidase (HRP), which can catalyze its colorless substrate tetrazmethyl benzidine (TMB) into a colored product. Hence, the concentration of Cu2+ can be evaluated visually for qualitative analysis according to the color change of the solution, and the optical density (OD) value of the resulting solution at 450 nm was also recorded using a microplate reader for quantitative analysis.
研究领域:
生物化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2023
·
IF 2.9
Qinhong Yin,Xiaolan Yang,Lihua Yang,Dezhi Yang,Yaling Yang,Yanqin Zhu
As newly developed synthetic enzymes with exceptional catalytic capabilities and outstanding stability, nanozymes have drawn considerable interest in the realm of sensing. Using a simple hydrothermal process, iodine and copper-doped carbon dots (Cu,I-CDs) with simulated enzymes were fabricated in the current investigation. Cu,I-CDs demonstrate peroxidase-mimicking function together with high catalytic effectiveness due to aforementioned features. This led to generation of a colorimetric sensor for quick and accurate quantitative assessment of morphine (MOR). The outcomes showed the method's usefulness for the colorimetric detection of MOR. The linear range for MOR detection is 0.25–25 μg/mL having a reduced detection limit of 64 ng/mL. This sensor's successful use in the analysis of MOR in biological material is more noteworthy.
研究领域:
生物传感
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纳米酶研究
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医学检测
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产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Gufeng Liu,Xiaoyi Wu,Lei Tan,Tiange Cheng,Chengyu Yang,Xin Liu,Fengxiao He,Ziqiao Tang,Qi Yan,Liangliang Fu,Min Wang,Haibin Xia,Tao Wu
Peri-implantitis is an inflammatory disease characterized by progressive bone loss, challenging implant longevity and incurring significant treatment burden. Current reconstruction therapy involves mechanical scaling followed by artificial bone grafting. However, residual bacteria and inflammation memory often lead to recurrence, highlighting the critical need for the artificial bone substitute with sustained anti-inflammatory and antibacterial properties. By utilizing the phosphate groups of mesoporous hydroxyapatites for in situ loading of CeO 2 , followed by a reduction method to fabricate a CeO 2 -Cu heterojunction interface, this study develops Bonyzymes with anti-inflammatory, antibacterial, and osteogenic properties. The incorporation of Cu modulates the electronic environment of the CeO 2 surface, increasing superoxide dismutase and catalase activities of Bonyzymes. In vitro, the Bonyzymes effectively regulated oxidative stress by scavenging excess reactive oxygen species, exerting anti-inflammatory effects. Additionally, it exhibits antibacterial activity against Staphylococcus aureus , Escherichia coli and Porphyromonas gingivalis , and facilitates osteogenic differentiation in bone marrow stem cells. In vivo experiments in rat peri-implantitis models confirm its ability to promote bone regeneration in the infected peri-implant area. Overall, this work develops novel artificial bone substitutes, Bonyzymes, which not only promotes bone regeneration but also exhibits anti-inflammatory and antibacterial properties, offering a promising strategy for peri-implantitis treatment.
研究领域:
生物材料
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口腔医学
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纳米技术
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炎症调控
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DOI
高影响因子
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
·
2023
·Vol. 28
·期 4
·379-391
·
IF 3
Chen Chan,Tang Weikang,Chen Qinfei,Han Mengqi,Shang Qi,Liu Wenbin
Hydroxytyrosol, one of the most powerful natural antioxidants, exhibits certificated benefits for human health. In this study, a biomimetic approach to synthesize hydroxytyrosol from the hydroxylation of tyrosol was established. EDTA-Fe 2+ coordination complex served as an active center to simulate tyrosine hydroxylase. H 2 O 2 and ascorbic acid were used as oxygen donor and hydrogen donor, respectively. Hydroxy radical and singlet oxygen contributed to active species. The biomimetic system displayed analogous component, structure, and activity with TyrH. Hydroxytyrosol titer of 21.59 mM, and productivity of 9985.92 mg·L −1 ·h −1 was achieved with 100 mM tyrosol as substrate. The proposed approach provided efficient and convenient route to quickly produce high amount of hydroxytyrosol. Graphical abstract
研究领域:
生物化学
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合成生物学
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抗氧化研究
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Zheng Yang,Qian Rong,Ting Bao,Mingzhi Jiao,Lebao Mao,Xiaojie Xue,Wei Wen,Zhen Wu,Xiuhua Zhang,Shengfu Wang
Among one-dimensional nanostructures , copper oxide nanotubes (CuO NTs) have aroused wide attention due to their prominent performance in electronic, optical and energy conversion devices. However, the lack of suitable synthetic methods limits its large-scale production and broad application. Here, a new method for preparing well-dispersed CuO NTs with simple operation, mild conditions and low cost was established by integrating the synergy effect of H + corrosion with the protection of CH 3 COO − and surfactant. Different surfactants were applied to regulate the synthetic process and polyvinylpyrrolidone-protected CuO NTs (PVP-CuO NTs) owned the best uniform morphology and dispersibility. The synthesis mechanism was deeply investigated to provide guidance for the synthesis of other oxide nanotube structures. As an excellent semiconductor material , CuO NTs can be excited to produce multiple electron-hole pairs under light irradiation , thus catalyzing the reduction of H 2 O 2 . CuO NTs modified photocathode realized photoelectrochemical sensing of H 2 O 2 in the range of 1 μM-1 mM. Interestingly, the prepared CuO NTs exhibited wonderful mimic enzyme properties, providing the possibility of colorimetric sensing and point-of-care detection. The colorimetric sensor for H 2 O 2 detection had a linear relationship from 2 μM to 150 μM with a detection limit of 0.62 μM. The successful construction of the two sensing modes not only demonstrated the excellent performance of multifunctional CuO NTs, but also indicated its great promise in practical application.
研究领域:
材料科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2022
·
IF 5.122
Changfang Lu,Yutong Wang,Wei Zhang,Yu Zhang,Yinyin Chen,Gang Xiang,Xianxiang Wang
The crosslinking of glutaraldehyde (GA) and nano-selenium coated chitosan (CS-SeNPs) afforded composites (GA–CS–SeNPs) which exhibit new properties. In addition to having mimic enzyme activity of CS-SeNPs, these composites exhibit fluorescence, which CS-SeNPs did not possess. The GA–CS–SeNPs can catalyze the chromogenic process of oxidation of substrate TMB at 20 °C and pH of 3.5, and under the excitation wavelength of 440 nm, the composites can emit strong fluorescence. The presence of mercury (II) (Hg 2+ ) inhibited the color process of catalytic oxidation of 3,3,5,5, -tetramethylbenzidine (TMB) and enhanced the fluorescence intensity of the composites to some extent. Therefore, using the fluorescence and mimicking enzyme properties of GA–CS–SeNPs, a fluorescent and colorimetric (dual-mode) determination of Hg 2+ was developed with good selectivity and high sensitivity. The linear determination ranges of Hg 2+ were 0–55 μM and 0–50 μM for fluorescent and colorimetric assays, respectively, and the limits of detection (LOD) for the dual-mode assay were as low as 0.34 μM and 0.40 μM, respectively. In addition, this new method was successfully implemented for dual-mode determination of Hg 2+ in real samples.
研究领域:
分析化学
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环境科学
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生物材料学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Die Deng,Shaohua Wen,Ya Wang,Jia Liu,Fang Li,Xiupei Yang
Cerium dioxide (CeO 2 ) has usually been used as a catalyst for colorimetric assays. However, the low catalytic efficiency has limited their further application. Covalent organic frameworks (COFs) have sparked increasing interest in the unique properties of large surface area, high porosity, and good thermochemical stability. Herein, a novel covalent organic framework composite (TpPa@CeO 2 ) with dual mimic enzyme activities (oxidase and peroxidase) was successfully synthesized via a facile hydrothermal method. TpPa@CeO 2 showed higher catalytic activity than CeO 2 . The excellent catalytic performance of TpPa@CeO 2 could be attributed to the higher surface area, more surface active species and higher Ce 3+ /Ce 4+ ratio in the composite than CeO 2 . Based on this, a visual and sensitive colorimetric sensor was constructed to detect ascorbic acid (AA) in the linear range of 0.2–35 μM with a low detection limit (LOD) of 0.061 μM. Moreover, the excellent colorimetric sensor was successfully applied to detect AA in beverage and tablet samples with satisfactory recoveries ranging from 95.78 % to 100.84 %. The catalytic mechanism could be that the composition of TpPa and CeO 2 improved the ratio of Ce 3+ /Ce 4+ , which promoted the decomposition of dissolved oxygen and H 2 O 2 by CeO 2 to produce superoxide radicals (·O 2 – ) and hydroxyl radicals (·OH), respectively. The facile and sensitive sensor system constructed by TpPa@CeO 2 with promising applications provides a new platform for the detection of AA in real sample analysis.
研究领域:
材料科学
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分析化学
·
生物医学
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产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 29
·13952-13962
·
IF 3.3
Dehong Bai,Ziyu Xue,Shaohui Li,Ran Meng,Dongxia Zhang,Xibin Zhou
Chromate (Cr(VI)) is a highly toxic heavy metal ion that not only endangers human health but also causes environmental pollution. To control and remove Cr(VI) from water more effectively, we report a simple, novel, and rapid detection technique for Cr(VI) in water based on an iron-manganese bimetal-metal organic framework (FexMny-MOF). Firstly, the synthesized FexMny-MOF has a large specific surface area and porosity; for the first time, an MOF with dual mimic enzyme properties is reported, which can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to the blue product in the presence or absence of H2O2. Interestingly, the mimic enzyme properties of FexMny-MOF are enhanced than those of Fe-MOF and Mn-MOF. The free energies of elementary reactions and adsorption energies (Eads) of H2O2 and O2 adsorbed on different materials were calculated by density functional theory (DFT) to verify the mechanism of action of FexMny-MOF. The Fe1Mn1-MOF mimics oxidase-like oxidation of TMB; 8-hydroxyquinoline (8-HQ) is used as the inhibitor of TMB oxidation, and the specific interaction between 8-HQ and Cr(VI) weakens the inhibition of 8-HQ on TMB oxidation to achieve the detection of Cr(VI). The established method has high sensitivity and selectivity with a detection limit of 51 nM and a linear range of 80–3500 nM. This method has been successfully applied to the determination of Cr(VI) in tap water and the Yellow River, showing its great potential in environmental monitoring.
研究领域:
环境科学
·
材料科学
·
生物化学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Xinyu Han,Jihong Zhou,Yunjie Li,Yuhan Zhao,Yanfei Li,Yingjie Hua,Taowen Dong,Fang Chai
An efficient and readable sensor is desirable for food safety and diagnosis. Herein, a homogeneous mimicking enzyme was constructed by encapsulating polyoxometalate (NH₄)₃[FeMo₆O₁₈(OH)₆]·6H₂O (FeMo 6 ) into the covalent organic framework (FeMo 6 @COF). Coordinating the spatial confinement effect of COF, FeMo 6 exhibited superior peroxide-like activity to catalyze H 2 O 2 to •O− 2, which achieved the “on-off” consecutive sensing of H 2 O 2 and AA via a readable colorimetric mode, with the limit of detection (LOD) at 30 μM and 0.35 μM, respectively. A convenient smartphone assistant platform was established and realized rapid, portable, visual monitoring of AA with LOD of 0.06 μM, and satisfied recoveries with 96.88–104.65 % in human serum and 98.37–107.61 % in commercial beverages. Furthermore, a logic gate circuit was designed to illustrate the potential utilization of combining the intelligent facilities with the experiment. This strategy provides efficient, swift, convenient sensor FeMo 6 @COF with great potential contribution in food safety and diagnosis.
研究领域:
生物传感器
·
食品安全
·
医学诊断
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产品关联
DOI
PubMed
高影响因子
Catalysts
·
2017
·Vol. 7
·期 10
·289
·
IF 3.082
Liwu Wang,Xiaonan Qu,Ying Xie,Shaowu Lv
Glutathione peroxidase is key for the removal of H2O2and other hydroperoxides and therefore, it has an important role in the maintenance of the reactive oxygen species (ROS) metabolic balance in vivo. The native enzymes of the glutathione peroxidase family (GPx) have many defects, such as instability in vitro and poor availability. GPx mimetics has become a topic of considerable interest in artificial enzyme research. Many forms of GPx mimics have been synthesized, by including selenium and tellurium (double-bridged and single-bridged, 2-substituted and 6-substituted) in a mother molecule but differences the GPx mimics enzymatic activity have rarely been compared. We designed and synthesized eight cyclodextrin derivatives and used two types of enzyme assays to determine their activities. The results show that: (a) tellurium-containing GPx mimics have higher activity than that of selenium-containing GPx mimics; (b) dual-bridged mimics have higher activity than bis-bridged mimics; and (c) 2-position modified cyclodextrin has higher activity than 6-position modified cyclodextrin.Keywords:glutathione peroxidase;mimic enzyme;cyclodextrin derivatives;enzyme activity;kinetics
研究领域:
生物化学
·
医学
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DOI
高影响因子
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