Sakura, a traditional edible flower, has been investigated for its anti-glycation potential; however, the bioactive components remain unclear. Polyphenols are recognized for their exceptional anti-glycation properties. In this study, main polyphenols were identified from seven varieties through metabolomics. 5-O-caffeoylquinic acid (5-OCA), 3-O-caffeoylquinic acid (3-OCA), and caffeic acid were confirmed as key agents through multivariate analysis, which was further validated by anti-glycation assessments. The concentrations of these polyphenols in Cerasus serrulata ‘Kanzan’ (CK) were 2.52 ± 0.08, 3.31 ± 0.18 and 2.38 ± 0.15 mg/g, respectively, contributing to its superior AGEs inhibition ratio (100 μg/mL) of 76.11 ± 0.40 %. Simulated digestion revealed that isomerization between 5-OCA and 3-OCA occurred during the gastric phase. Both compounds were metabolized to caffeic acid in the intestinal phase, contributing to a sustained anti-glycation activity of 64.74 ± 1.11 %. Overall, our study provides a theoretical basis for the development of Sakura-based functional foods targeting anti-glycation.
Safety concerns arising from bacteria present a significant threat to human health, underscoring the pressing need for the exploration of novel antimicrobial materials. Nanozymes, as a new type of nanoscale material, have attracted widespread attention for antibacterial applications owing to their ability to mimic the catalytic activity of natural enzymes. In this work, we have constructed copper-doped cherry blossom carbon dots (Cu-CDs) with excellent peroxidase-like (POD) activity using a one-pot hydrothermal method. The utilization of cherry blossom as a natural material precursor significantly enhances its biocompatibility. Furthermore, the incorporation of copper ions initiates Fenton-like reaction-triggered POD-like catalytic activity, effectively eradicating bacteria by converting hydrogen peroxide (H2O2) into hydroxyl radicals (·OH). The antibacterial test results demonstrate that Cu-CDs exhibit a bactericidal efficacy of over 90% against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). This study presents a novel environmentally friendly nanozyme material derived from natural sources, exhibiting significant antimicrobial properties and offering innovative insights for the advancement of antimicrobial materials.
Lushan Yunwu tea (LSYWT) is a famous green tea in China. However, the effects of intercropping tea with flowering cherry on the overall aroma of tea have not been well understood. In this study, headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography–mass spectrometry (GC–MS) was used for analysis. A total of 54 volatile compounds from eight chemical classes were identified in tea samples from both the intercropping and pure-tea-plantation groups. Principal component analysis (PCA), orthogonal partial least-squares discriminant analysis (OPLS-DA), and odor activity value (OAV) methods combined with sensory evaluation identified cis-jasmone, nonanal, and linalool as the key aroma compounds in the intercropping group. Benzaldehyde, α-farnesene, and methyl benzene were identified as the main volatile compounds in the flowering cherry using headspace solid-phase microextraction/gas chromatography–mass spectrometry (HS-SPME/GC–MS). These findings will enrich the research on tea aroma chemistry and offer new insights into the product development and quality improvement of LSYWT.
危险品化学品经营许可证（带存储）