CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Xing Han,Xin Tang,Rifei Chen,Wei Li,Pingan Zhu,Liqiu Wang
We present a citrus-peel-like micro-cavity structure for the design of durable Slippery Liquid-Infused Porous Surfaces (SLIPSs). Owing to the re-entrant and enclosed nature of micro-cavity, lubricant oils can be stabilized by mitigating mass loss resulting from liquid displacement and shear drainage, respectively. Citrus-peel-like micro-cavity SLIPS displays two orders of magnitude enhancement in durability in the droplet-sliding test when compared with micro-pillar SLIPS and smooth lubricant surface. We demonstrated precise droplet manipulation without liquid loss and cross-contamination by harnessing the Marangoni effect with photo-thermal control. Cavity-smooth SLIPS with large adhesion contrast was patterned to initiate multiple droplet coalescences for on-demand neutralization reaction. The citrus-peel-like micro-cavity SLIPS would be beneficial to various applications, such as multi-step chemical reactions and bio-detections that require loss-free, contamination-free, and programmable droplet manipulation.
研究领域:
材料科学
·
表面工程
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2022
·
IF 11.504
Weiwei Hu,Huan Cheng,Dongmei Wu,Jianle Chen,Xingqian Ye,Shiguo Chen
The rhamnogalacturonan I (RG-I) region of pectin shows health benefits and novel gel applications. Commercial pectin is extracted from citrus peel in most instances, whereas they are homogalacturonan (HG)-dominated productions and the methods to extract pectin targeting RG-I are limited. To take full advantage of citrus wastes and upgrading this pectin resources, a new extraction method based on a diluted NaOH assisted by a combination of ultrasound (US) and pressure, namely manosonication (MS), was established for RG-I enriched pectin extraction. MS significantly enhanced the pectin yield (21.47 ± 0.11%) while reducing the use of NaOH reagents (50 mmol/L) and processing time (50 min). Structurally, the MS (300 kPa) resulting products achieved nearly a 2-fold greater RG-I content (58.61 mol%) than that of commercial pectin (30.12 mol%). Moreover, for the investigation of the extraction mechanism, external pressure enhanced the cavitation effect, as the calorimetric intensity increased from 1.28 W/mL (100 kPa) to 3.3 W/mL (350 kPa). A sufficient ultrasonic mechanical action allowed for obtaining RG-I enriched products with large molecular weight (9.506 × 10 4 –2.813 × 10 5 ) and low degree of methoxylation. Transmission electron microscopy (TEM) and scanning electron microscopies (SEM) verified the increased disintegration of the cell wall tissue by MS. Raman imaging and immunofluorescence labeling suggested the synergistic effect of pressure and ultrasound on enhancing the yields and RG-I content. This new method provided alternations in functional polysaccharides extraction, and the yield and structure of citrus RG-I enriched pectin strongly indicates the potentials of MS in other RG-I rich vegetables applications.
研究领域:
食品科学
·
植物化学
✓ 已验证
DOI
高影响因子
Journal of Translational Medicine
·
2025
·Vol. 23
·期 1
·1-19
·
IF 7.5
Zhao Zhiqi,Deng Yixuan,Li Li,Zhu Liying,Wang Xin,Sun Haibiao,Li Xiaoqiong,Han Xiaoqiang,Li Jinjun
Background Phytohormones have garnered considerable interest as potential modulators of the gut-bone axis. Denosumab (Deno), a widely utilized therapeutic agent for postmenopausal osteoporosis, has not been previously investigated for its effects on gut health. The objective of this study was to assess the efficacy of isoflavones (SI), naringin (Nar), and Deno in the management of postmenopausal osteoporosis by targeting the gut-bone axis.Methods The postmenopausal osteoporosis model in mice was established via bilateral oophorectomy. Subsequently, mice in the Deno group received subcutaneous injections of Deno at a dosage of 10 mg/kg, administered twice weekly. In contrast, mice in the SI and Nar groups were subjected to oral gavage with 200 mg/kg/day of SI and Nar, respectively. The treatment period for all groups lasted for 8 weeks. Upon the conclusion of the experiment, a thorough evaluation of the effects of SI, Nar, and Deno on bone and gut health in mice was conducted through immunological, pathological, imaging, and multi-omics methodologies.Results Deno, SI, and Nar significantly alleviated the physical symptoms in postmenopausal mice. However, only SI and Nar significantly modulated the gut microbiota. Akkermansia was significantly enriched after the gavage of SI and Nar. Akkermansia has the capacity to not only augment bone mass and alleviate strength deterioration via extracellular vesicles, but it also influences bone metabolism by diminishing inflammation and modulating lipid metabolism. Notably, no significant changes in the gut microbiota were observed in the Deno group, which may be attributed to the differences in the method of administration, as Deno was administered via subcutaneous injection rather than gavage.Conclusion SI and Nar may influence the gut–bone axis through Akkermansia and have the potential of alternative treatment options for postmenopausal osteoporosis. Although the gut microbiota is not significantly affected by the subcutaneous administration of Deno, the long-term management of postmenopausal osteoporosis and the exploration of various management models warrant additional scrutiny. Furthermore, this study has yet to establish a dose–response relationship, indicating that further research is essential to clarify the regulatory effects of varying doses of SI and Nar on postmenopausal osteoporosis especially the modulation of gut microbiota.Graphical
研究领域:
医学
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微生物学
·
营养学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Na Yang,Yamei Jin,Yuyi Zhou,Xiaoqun Zhou
A novel electromagnetic heat extraction method was presented, whereby mandarin peels residue solution was located in a winding coil subjected to an oscillating magnetic field, and the pectin was extracted under appropriate conditions. Numerical relationships between applied magnetic field and induced electric field (IEF) in the extraction process were elaborated. The results showed that the induced current density, IEF and terminal temperature increased with increasing magnetic field. The maximum current density of 0.35 A/cm corresponds to the highest terminal temperature of 84.6 °C and IEF intensity of 26.6 V/cm. When magnetic field intensity was 1.39 T and the extraction time was 15 min, the maximum yield of pectin reached 9.16 %. In addition, all treatments impacted the ash content, protein content, water-holding capacity (WHC), and oil-holding capacity (OHC) of the obtained pectin. The pectin extracted by electromagnetic heat had the lowest DE value of 71.3 % with 126.55 kDa molecular weight, while the GalA content was at the highest level of 76.18 %. After different treatments, the composition of pectin monosaccharides changed, but there were slight differences in the composition of pectin polysaccharides. Moreover, the electromagnetic heat extracted pectin had light color and an obvious surface fragmentation of the peel residue.
研究领域:
食品科学
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化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Essential Oil Bearing Plants
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2022
·
IF 1.971
Weilong Zheng,Fang Wu,Xi Luo,Caimiao Bian,Yingying Zhang,Fengwei Yin,Haiyu Liu,Yongqian Fu
Large amounts of citrus peel are discarded as solid waste owing to the lack of recycling methods. In this study, mixed solvent extraction combined with multi-phase salting-out extraction was performed to explore the extraction process of essential oil and hesperidin. Optimal conditions were used for mixed solvent extraction (citrus peel 6%; ratio of ethanol to n-hexane 1:5; duration, stirring speed, and temperature were 150 min, 120 r/min, and 45°C, respectively) and multi-phase salting-out extraction (citrus peel residue 10%; ratio of salt to ethanol 19:24; mass fraction of n-hexane 25%; extraction duration 120 min). Under two-step extraction conditions, 63.74±2.40% essential oil and 27.13±1.36% hesperidin were recovered, with total extraction efficiencies of 5.19±0.09 and 1.91±0.25%, respectively. The amplification effect was not significant, and the organic solvents could be reused three times while maintaining 80% recovery in both steps to balance the commercial cost and extraction efficiency.
研究领域:
食品科学
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植物化学
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Zihui Jin,Di Sun,Yifan Liu,Bo Xu,Yaqi Jin,Qiyang Zhao,Yue He,Jing Li,Yaohai Zhang,Yongliang Cui
Tangeretin, a polymethoxyflavone abundant in citrus fruits, is acclaimed for its diverse biological benefits to human health and is a significant component of traditional Chinese medicine. This study reports the development of an indirect competitive enzyme-linked immunosorbent assay (icELISA) with monoclonal antibody for quantifying tangeretin in various citrus and herbal medicines. The optimized icELISA demonstrated a half-maximal inhibitory concentration (IC 50 ) of 5.57 ng/mL, encompassing a dynamic range of 1.54–25.38 ng/mL, and showcased average recoveries ranging from 81.65 % to 105.75 % in citrus peel and pulp samples. Extensive analysis of six citrus cultivar samples and four herbal samples was conducted using the developed assay, with results corroborated by Ultra–Performance Liquid Chromatography Tandem Mass Spectrometry (UPLC–MS/MS). These findings confirm the assay’s efficacy and reliability for tangeretin detection, offering a practical method for evaluating this bioactive compound in food.
研究领域:
食品科学
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免疫学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FISH DISEASES
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2021
·Vol. 44
·期 10
·1503-1513
·
IF 2.767
Zhong-Chen Sun,Cheng Chen,Fei-Fan Xu,Bing-Ke Li,Jing-Lei Shen,Tao Wang,Hai-Feng Jiang,Gao-Xue Wang
White spot syndrome virus (WSSV) is a serious pathogen threatening global crustacean aquaculture with no commercially available drugs. Herbal medicines widely used in antiviral research offer a rich reserve for drug discovery. Here, we investigated the inhibitory activity of 13 herbal medicines against WSSV in crayfish Procambarus clarkii and discovered that naringenin (NAR) has potent anti-WSSV activity. In the preliminary screening, the extracts of Typha angustifolia displayed the highest inhibitory activity on WSSV replication (84.62%, 100 mg/kg). Further, NAR, the main active compound of T. angustifolia, showed a much higher inhibition rate (92.85%, 50 mg/kg). NAR repressed WSSV proliferation followed a dose-dependent manner and significantly improved the survival of WSSV-challenged crayfish. Moreover, pre- or post-treatment of NAR displayed a comparable inhibition on the viral loads. NAR decreased the transcriptional levels of vital genes in viral life cycle, particularly for the immediately early-stage gene ie1 . Further results showed that NAR could decrease the STAT gene expression to block ie1 transcription. Besides, NAR modulated immune-related gene Hsp70 , antioxidant ( cMnSOD, mMnSOD, CAT, GST ), anti-inflammatory ( COX-1 , COX-2 ) and pro-apoptosis-related factors (Bax and BI-1) to inhibit WSSV replication. Overall, these results suggest that NAR may have the potential to be developed as preventive or therapeutic agent against WSSV.
研究领域:
水产养殖学
·
病毒学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Fuchao Zhan,Xiangxing Yan,Jing Li,Feng Sheng,Bin Li
In this work, water-resistant poly (vinyl alcohol) (PVA)/poly (acrylic acid) (PAA) electrospun fibers encapsulating tangeretin (Tan) were fabricated by emulsion-electrospinning. To optimize the electrospinning condition, the size and morphology of electrospun fibers were characterized by dynamic light scattering (DLS), optical light microscope, fluorescence microscopy, and scanning electron microscopy (SEM), respectively. The optimized initial concentration of PVA/PAA was 10% (w/w) with a mass ratio of 3:7. The time and temperature of optimized thermal crosslinking treatment were 2 h and 145 °C, respectively. The results of XRD and SEM showed that the Tan was successfully incorporated into smooth PVA/PAA electrospun fibers and those fibers possessed nano-diameter size and high porosity. The encapsulation of Tan had no significant impact on the antioxidant activity of PVA/PAA/Tan crosslinking electrospun fibers. The in vitro release test showed the PVA/PAA/Tan crosslinking electrospun fibers achieved durable release profiles and lower burst release rates than that from the pure Tan emulsion. Based on these results, it is concluded that PVA/PAA/Tan crosslinking electrospun fibers prepared by emulsion-electrospinning serve as a promising technique in the fields of water-insoluble drug delivery and slow-release.
研究领域:
材料科学
·
药物递送
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Xin Huang,Junxiang Zhu,Li Wang,Huijuan Jing,Chaoyang Ma,Xingran Kou,Hongxin Wang
This study aimed to reveal the interaction and inhibitory mechanisms of tangeretin (TAN), nobiletin (NBT), and their acidic hydroxylated forms, 5-demethyltangeretin (5-DT) and 5-demethylnobiletin (5-DN) on porcine pancreatic lipase (PPL) using spectroscopic techniques and molecular dynamics (MD) simulation. PPL inhibition assay showed that the inhibitory activity of NBT (IC 50 value of 3.60 ± 0.19 μM) was superior to those of three polymethoxylated flavones (PMFs), indicating it may be related to the methoxy groups at the 3′-position in its molecular structure. Inhibition kinetic analyses demonstrated that the inhibition types of the 4 PMFs were consistent with the mixed inhibition model, which agreed well with the results from the ultraviolet-visible (UV–Vis) spectroscopy, Circular dichroism (CD), fluorescence spectroscopy, molecular docking , and MD simulation that PMFs could bind to the PPL catalytic site and non-catalytic site, affecting the normal spatial conformation of PPL and weakening its ability to decompose the substrate. All these findings suggest that PMFs are a kind of natural lipase inhibitors, and NBT has the potential as a lipase inhibition precursor because of its unique flavone skeleton structure.
研究领域:
生物化学
·
药理学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2025
·
IF 2.2
Ying He,Meng-Hui He,Tingting Jin,Siju-Li,Hua-Qiao Wang,Feng He
Tangeretin, a flavonoid from Citri Reticulatae Pericarpium, is known for its neuroprotective effects, but the mechanisms are not fully understood. Alzheimer's disease, a leading neurodegenerative disorder, characterized by amyloid-beta (Aβ) accumulation, represents a significant therapeutic challenge. This study investigates the protective effects of tangeretin against Aβ1-42-induced neurotoxicity using HT22 cells and zebrafish larvae as experimental models. Tangeretin mitigated Aβ 1-42 -induced cytotoxicity, as evidenced by enhanced cell viability and reduced apoptosis. Tangeretin treatment mitigated Aβ 1-42 -induced cytotoxicity in HT22 cells, as evidenced by enhanced cell viability and reduced apoptosis. Mechanistically, tangeretin ameliorated mitochondrial dysfunction by reducing mitochondrial fragmentation, decreasing donut-shaped mitochondria, restoring mitochondrial membrane potential, and attenuating reactive oxygen species (ROS) production. Moreover, tangeretin modulated mitochondria-lysosome interactions by promoting mitophagy and normalizing the prolonged mitochondria-lysosome contact induced by Aβ 1-42 . In zebrafish larvae, Aβ 1-42 exposure resulted in developmental malformations, including pericardial and yolk sac edema, elevated ROS levels, increased apoptosis, and impaired neurodevelopment. Tangeretin effectively counteracted these deficits, as revealed by live imaging, supporting its neuroprotective role observed in cellular models. Collectively, our study suggests that tangeretin may serve as a promising protective agent against Aβ 1-42 -induced neurotoxicity.
研究领域:
神经科学
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细胞生物学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Energy Science & Engineering
·
2021
·Vol. 9
·期 7
·1033-1041
·
IF 4.17
Hao Xu,Yuan Zhao,Yingsha Guan,Chenguang Wang,Lingjun Zhu,Shanshan Xiong,Shurong Wang
In recent years, there has been a great interest in the conversion from agricultural waste to high value-added chemicals. Citrus peel is a low-cost and widely available waste resource that can be derived during citrus processing. In this study, we analyzed the composition of typical citrus peel and its hydrolysate. The hydrothermal conversion of citrus peel in different reaction conditions was also studied, and the optimum conditions for the preparation of levulinic acid and furfural were obtained. The maximum yield of levulinic acid was 21.9 wt% obtained in pure water, and the maximum yield of furfural was 9.1 wt% obtained in the water-DMSO solvent mixture. With the increase in the reaction temperature, the yield of furfural decreased while the yield of levulinic acid increased.
研究领域:
化学工程
·
环境科学
·
生物化学
✓ 已验证
DOI
高影响因子
Foods
·
2022
·Vol. 11
·期 17
·2662
·
IF 5.561
Xiangying Yu,Xiaochun Chen,Yuting Li,Lin Li
To reflect the volatile differences of dried citrus peel as affected by cultivars and drying methods, the volatile compounds of dried citrus peel of two cultivars (Citrus reticulata“Chachi” andCitrus reticulata“Ponkan”), prepared under three drying methods (sun-drying (SD), hot-air-drying (AD), and freeze-drying (FD)), were analyzed by GC-MS, odor activity values (OAVs), and GC-IMS. GC-MS data indicated that SD was favorable to preserve terpenic alcohols (linalool, α-terpineol and terpinene-4-ol), β-cymene, methyl methanthranilate, and monoterpenes; while AD was favorable to preserve aliphatic aldehydes and sesquiterpenes; and SD was more similar with AD in GC-MS analysis of volatile profile (of higher MW) for both cultivars from the PCA outcome. Furthermore, significant difference in volatile isomeric composition of different samples was also clearly demonstrated through extracted ion chromatogram (EIC) by GC-MS analysis. GC-IMS analysis showed the favorability of FD to preserve ketones, phenols, esters, and aromatic aldehydes; and SD was more similar with FD in GC-IMS analysis of volatile profile (of smaller MW) for both cultivars from the PCA outcome. Moreover, the OAVs indicate that 2-methoxy-4-vinylphenol contributed much to the flavor of dried Ponkan peel, while 2-methoxy-4-vinylphenol, methyl methanthranilate, and methyl anthranilate played an important role in the flavor of dried Chachi peel; and the highest OAVs for monoterpenes were observed at SD for both cultivars. Thus, the combination of GC-MS and GC-IMS analyses with PCA in this paper suggested the superiority of SD to preserve volatiles and characteristic aroma in dried citrus peel, and that SD contributed much to the quality of dried Chachi peel.Keywords:citrus peel;volatile compounds;sun-drying (SD);hot-air-drying (AD);freeze-drying (FD);GC-MS;GC-IMS
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）