Horticultural Plant Journal
·
2021
·
IF 3.032
Haoliang Wan,Xiaoliang Zhang,Ping Wang,Haiji Qiu,Yafei Guo,Yunjiang Cheng,Weiwei Wen
Sesquiterpene valencene is dominant in flavedo tissues of sweet oranges and imparts a unique woody aroma. However, the interaction between the biosynthetic pathways of valencene and other nutritional compounds is less studied. Sesquiterpenoids were significantly accumulated in a previously reported glossy mutant of orange (MT) than the wild type (WT), especially valencene and caryophyllene. In addition, we identified several other pathways with variations at both the transcriptional and metabolic levels in MT. It's interesting to found those up-regulated metabolites in MT, such as eukaryotic lipids, kaempferol and proline also showed strong positive correlation with valencene along with fruit maturation while those down-regulated metabolites, such as phenylpropanoid coumarins and most of the modified flavonoids exhibited negative correlation. We then categorized these shifted pathways into the ‘sesquitepenoid-identical shunt’ and the sesquitepenoid-opposite shunt’ and confirmed the classification result at transcriptional level. Our results provide important insights into the connections between various fruit quality-related properties.
研究领域:
植物学
·
分子生物学
·
代谢组学
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DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Hang Liu,Chen Yang,Xiaolin Wu,Shengfeng Peng,Lei Zhou,David Julian McClements,Wei Liu
Plant-derived extracellular vesicles have considerable potential as natural pharmaceutical and nutraceutical delivery systems. However, the impact of plant maturity on the physicochemical and structural properties of isolated extracellular vesicles is currently unknown. In this work, extracellular vesicles isolated from oranges at different maturity stages were first characterized and compared. Afterwards, polyphenol-load orange juices were successfully prepared by incorporating polyphenols (mainly curcumin) into extracellular vesicles originated from orange juices. Encapsulation in vesicles was found to increase the solubility, stability, bioaccessibility, and antioxidant activity of curcumin, but the effects depended on the maturity of oranges. Specifically, the vesicles from unripe and ripe orange juices were more effective for curcumin delivery than those from overripe orange juice. Conclusively, this study has provided important new information about the optimum maturity for isolating fruit-derived extracellular vesicles. Moreover, the extracellular vesicle-based delivery systems developed in this study may facilitate the design of more effective functional foods and beverages.
研究领域:
食品科学
·
植物学
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产品关联
DOI
PubMed
高影响因子
Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment
·
2022
·
IF 3.549
Yanqun Tong,Liuxin Ding,Kuiguo Han,Xiaobo Zou,Shiyan Wang,Zhang Wen,Yunxia Ye,Xudong Ren
A novel microfluidic metal grating integrated terahertz sensor has been designed, which is composed of a metal microstructure array-dielectric layer-metal layer, where the dielectric layer is the microfluidic channel carrying the analyte. By adjusting the structural parameters of the metal grating sensor, a highly confined electromagnetic field can be obtained in the microfluidic channel, thereby significantly enhancing the interaction between the analyte and the terahertz wave and improving the terahertz detection sensitivity. The metal grating described in this paper is composed of an array of square holes, which is manufactured using laser micromachining technology, so that the measurement method is simplified and improved. The results show that the addition of different concentrations of carbendazim solution resulted in a redshift of the overall spectrum, with the highest sensitivity reaching 8.773 GHz/mg L−1, which is about eight times more sensitive than the traditional terahertz transmission sensor. The relative error of using this method to determine carbendazim levels in orange juice samples was less than 5.3%. The terahertz time-domain spectroscopy technology combined with the metal grating integrated microfluidic sensor can improve the sensitivity of sample detection and realize the rapid detection and analysis of trace elements.
研究领域:
食品科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Hang Liu,Jie Liu,Saidi ji,Shengfeng Peng,Lei Zhou,Wei Liu
Plant-derived extracellular vesicles have presented great potential in drug and/or nutrition delivery, but it is still unclear whether the variety affects the physicochemical properties of plant derived extracellular vesicles. In this work, the extracellular vesicles from various oranges were first characterized, including navel orange juice (NOJ), green orange juice (GOJ), bingtang orange juice (BTOJ) and blood orange juice (BOJ). The results exhibited obvious distinctions of extracellular vesicles among different oranges, such as vesicle concentration, surface potential, lipid composition, protein content and so on. Furthermore, the polyphenol-loaded orange beverages were successfully prepared via incorporating polyphenols into the orange-derived extracellular vesicles in an amorphous form. Taking curcumin (Cur) as an example, extracellular vesicles could confer it with the higher solubility, stability and bioaccessibility after encapsulation. Conclusively, the extracellular vesicles in NOJ exhibited more satisfactory results for curcumin delivery than those in the other three orange juices. In particular, one-step prepared polyphenol-rich orange beverages have higher antioxidant ability than blank orange beverages regardless of the influence of fruit variety. This suggests that using extracellular vesicle as natural nanocarrier is a feasible method for constructing the full plant-based functional food. Additionally, the current study will provide a meaningful information for the selection of plant-derived extracellular vesicle in the future research.
研究领域:
植物学
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食品科学
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生物化学
·
药剂学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2012
·
IF 4.531
Hongyuan Yan,Fang Wang,Hui Wang,Gengliang Yang
A simple, convenient and high selective miniaturized molecularly imprinted matrix solid-phase dispersion (mini-MI-MSPD) technique using l -tryptophan-imprinted polymers as sorbents was proposed for simultaneous extraction of four auxins (indole-3-acetic acid (IAA), indole-3-propionic acid (IPA), indole-3-butyric acid (IBA) and 1-naphthaleneacetic acid (NAA)) from orange samples coupled with high performance liquid chromatography–ultraviolet detection. The molecularly imprinted polymers (MIPs) synthesized using l -tryptophan as dummy template showed high affinity and selectivity to auxins, and so they were applied as the specific dispersant of MSPD to simultaneously eliminate the effect of template leakage on quantitative analysis. Under the optimized conditions, good linearity was obtained in a range of 0.02–2.00 μg g −1 and the average recoveries of four auxins at three spiked levels ranged from 87.6% to 104.5% with the relative standard deviation (RSD) ≤4.4%. The presented mini-MI-MSPD method combined the advantages of MSPD for allowing the extraction, dispersion and homogenization in a single step and the advantages of MIPs for high affinity and selectivity towards four auxins, which could be applied to the determination of auxins in complicated vegetal samples.
研究领域:
植物学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Jie Liu,Xinxin Xu,Aihong Wu,Shanshan Song,Liguang Xu,Chuanlai Xu,Hua Kuang,Liqiang Liu
Etoxazole, a novel organofluorine pesticide, has been widely used against various mites in fruits and vegetables. In this study, we designed an etoxazole-hapten and produced a highly sensitive monoclonal antibody (mAb) against etoxazole with a half-maximal inhibitory concentration of 4.04 ng/mL. Based on the mAb, we developed an immunochromatographic assay (ICA) for the detection of etoxazole in orange and grape samples. In the qualitative detection, the visible limit of detection (vLOD) and cut-off value were 10 and 100 ng/g (fresh weight), respectively. In the quantitative detection, the LOD value was 2.03 ng/g (fresh weight) in orange and 2.52 ng/g (fresh weight) in grape samples. The recovery rates ranged from 94.7 ± 4.0% to 103.3 ± 3.8%, with CVs of 3.7–4.2% and 96.4 ± 3.4% to 106.1 ± 5.5%, with CVs of 3.5–5.2% for etoxazole in grape and orange samples, respectively. The ICA results were similar to those of LC-MS/MS. Therefore, our developed ICA is a rapid, sensitive, and simple tool for screening etoxazole residues in fruits and vegetables.
研究领域:
分析化学
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食品科学
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环境科学
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DOI
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Abdul Qayum,Arif Rashid,Asif Ali Khan,Mingming Zhong,Yuxuan Liu,Muhammad Hussain,Muhammad Safiullah Virk,Zahoor Ahmed,Abdur Rehman,Abid Hussain,Qiufang Liang,Xiaofeng Ren,Haile Ma,Ekram Abd El-Salam,Song Miao
Tri-frequency ultrasound (TFU) at 20-40-60 kHz was used to systematically examine the effects on the multi-scale network structure of an emulsion gel containing orange essential oil prepared with glycosylated whey protein (WP). Treatment intervals of pre-and-post 10, 20, and 30 min were utilized to evaluate the influence and interactions on functional (particle size, zeta-potential), rheological (storage modulus (G′) and loss modulus (G″), and oxidative stability (conjugated diene and peroxide value). The optimized tri-frequency pre- and post-treated sample demonstrated enhanced physical stability as compared to the un-treated sample, as evidenced by droplet size, (from 468.14 ± 0.56 to 214.21 ± 0.42 nm), ζ-Potential (mV) (from −29.67 ± 0.31 to −39.98 ± 1.42), and structural morphology. Structure analysis using FTIR that the aforementioned improvements were made possible through TFU enhanced hydrogen bonds, molecular arrangements with greater order, and improved intermolecular compatibility. XRD results showed gradual shift from 8.32° to 8.64° and from 19.2° to 19.5°, respectively. TFU post-treatment gels exhibited higher viscosity, more pronounced pseudoplastic behavior compare to pre-treatment, attributed to strong interaction forces and three-dimensional gel network. Our results provide valuable insights into the application of multi-frequency ultrasound as a promising technique for designing protein emulsion gels tailored for nutrient delivery systems.
研究领域:
食品科学
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材料科学
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生物物理学
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产品关联
DOI
高影响因子
Polymers
·
2021
·Vol. 14
·期 1
·85
·
IF 4.329
Duy-Nam Phan,Muhammad Qamar Khan,Van-Chuc Nguyen,Hai Vu-Manh,Anh-Tuan Dao,Phan Thanh Thao,Ngoc-Mai Nguyen,Van-Tuan Le,Azeem Ullah,Muzamil Khatri,Ick-Soo Kim
This study demonstrated a controllable release properties and synergistic antibacterial actions between orange essential oil (OEO) and silver nanoparticles (AgNPs) incorporated onto cellulose (CL) nanofibers. The preparation of AgNPs attached on CL nanofibers was conducted through multiple processes including the deacetylation process to transform cellulose acetate (CA) nanofibers to CL nanofibers, the in situ synthesis of AgNPs, and the coating of as-prepared silver composite CL nanofibers using OEO solutions with two different concentrations. The success of immobilization of AgNPs onto the surface of CL nanofibers and the incorporation of OEO into the polymer matrix was confirmed by SEM-EDS, TEM, XRD, and FT-IR characterizations. The tensile strength, elongation at break, and Young’s modulus of the nanofibers after each step of treatment were recorded and compared to pristine CA nanofibers. The high antibacterial activities of AgNPs and OEO were assessed against Gram-positiveB. subtilisand Gram-negativeE. colimicroorganisms. The combined effects of two antimicrobials, AgNPs and OEO, were distinctively recognized againstE. coli.Keywords:cellulose nanofiber;silver nanoparticle;electrospinning;orange essential oil;antibacterial activity
研究领域:
材料科学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Qianqian Lu,Liqiang Liu,Jinyan Li,Shanshan Song,Hua Kuang,Chuanlai Xu,Lingling Guo
Amitraz (AMT) is a broad-spectrum formamidine insecticide and acaricide. In this study, we produced an anti-AMT monoclonal antibody (mAb) with high performance. The half-maximal inhibitory concentration of the anti-AMT mAb was 4.418 ng/mL, the cross reactivity with other insecticides was negligible, and an affinity constant was 2.06 × 10 9 mmol/L. Additionally, we developed an immunochromatographic assay for the rapid detection of AMT residues in oranges, tomatoes, and eggplants. The cut-off values were 2000 μg/kg in oranges and tomato samples and 1000 μg/kg in eggplant samples and the calculated limits of detection were 14.521 μg/kg, 6.281 μg/kg, and 3.518 μg/kg in oranges, tomatoes, and eggplants, respectively, meeting the detection requirements for AMT in fruits and vegetables. The recovery rates ranged between 95.8 % and 105.2 %, consistent with the recovery rates obtained via LC-MS/MS. Our developed immunochromatographic assay can effectively, accurately, and rapidly determine AMT residues in oranges, tomatoes, and eggplants.
研究领域:
农业科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Xiong-Xin Peng,Guang-Ming Bao,Yu-Fei Zhong,Li Zhang,Kang-Bo Zeng,Jia-Xin He,Wei Xiao,Yi-Fan Xia,Qing Fan,Hou-Qun Yuan
Thiabendazole (TBZ), has been extensively employed as a pesticide and/or a fungicide in agriculture, while its residues would threaten to public health and safety. Simple, rapid and sensitive probes for detection of TBZ in real food samples is significantly desirable. In present work, a highly selective and sensitive luminescent sensor for monitoring TBZ in oranges has been constructed based on a Tb 3+ -functionalized Zr-MOF (Tb 3+ @ 1 ). Tb 3+ @ 1 exhibited many attractive sensing properties toward TBZ, including broad linear range (0–80 μM), high selectivity, low LOD (0.271 μM) and rapid response time (less than1 min). Moreover, the probe was employed to determine TBZ in real orange samples, in which good recoveries from 98.41 to 104.48% were obtained. It only takes 35 min for the whole process of detection TBZ in real orange samples combined with QuEChERS method. Therefore, this work provided a reliable and rapid method for monitoring the TBZ in real orange samples.
研究领域:
食品科学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Yang Wang,Yuling Zhao,Rongrong Wu,Jingwen Gao,Menghan Chen,Yue Cui,Jianxiong Hao,Junhua Han,Karl Matthews
This study investigated the antifungal effect of curcumin-mediated photodynamic inactivation (PDI) with ascorbic acid (Vc) as a synergist on Penicillium italicum and its biofilms, and evaluated the efficacy in inactivating P. italicum on fresh-cut orange. Results showed that the number of P. italicum spores decreased by 4.61 Log CFU/mL when the concentration of curcumin, Vc and illumination time was 75 μmol/L, 250 mg/L and 20 min, respectively. After PDI treatment, the cell structure of P. italicum was destroyed and leakage of cell contents was observed. Mature P. italicum biofilms were formed after 72 h of incubation, and PDI could reduce the spores in biofilms by 3.23 Log CFU/mL. In addition, under the optimal photodynamic conditions, the number of P. italicum spores in fresh-cut orange decreased by 2.48 Log CFU/g, and the sensory quality was well maintained. In conclusion, curcumin-mediated photodynamic technology could better maintain the freshness of fresh-cut orange. Therefore, this study deepened the understanding of the antimicrobial efficacy of curcumin-mediated PDI on food borne pathogens and its impact on food quality.
研究领域:
食品科学
·
微生物学
·
植物病理学
✓ 已验证
产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Zikai Li,Yuhe Dong,Tong Wu,Wanying Zhu,Haohan Jing,Tao Jiang,Yao Liu,Xi Yu,Ying Xiao,Tian Zhong
To reduce food contamination caused by foodborne pathogens, improve encapsulation stability and targeting efficacy of antimicrobial agents, and minimize the required dosage, we developed a pectin-coated liposome containing thymol (Thy@Lip@Pec). The pectin shell of this formulation can be enzymatically degraded by pectinase secreted by fungi, thereby triggering the on-demand release of thymol. Under the action of pectinase at 1.0 mg/mL, this formulation released 76.45 % of thymol within 6 h. Thy@Lip@Pec treatment significantly inhibited Aspergillus niger and Penicillium spp. colonies on agar plates. Moreover, while maintaining quality indicators of oranges, Thy@Lip@Pec significantly suppressed postharvest diseases caused by A. niger and Penicillium spp., reducing the decay incidences of A. niger from 80.00 % (control) to 6.67 %, and that of Penicillium spp. from 93.33 % (control) to 33.34 % in artificially inoculated oranges. This on-demand release antimicrobial material system represents an advanced and efficient strategy for food preservation.
研究领域:
食品科学
·
微生物学
·
纳米技术
·
农业科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）