FOOD HYDROCOLLOIDS
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2023
·
IF 10.7
Haihua Jiang,Xiaoyun Fei,Guowen Zhang,Xing Hu,Deming Gong,Junhui Pan
Hesperitin (Ht) and hesperidin (Hd) have a variety of biological activities, but their applications are limited due to the poor aqueous dispersion, weak stability and low bioavailability. In this study, a complex coacervate, formed by glutenin heated at pH 2, 80 °C for 8 h and orange peel pectin (P), was used as a carrier to enhance the water dispersibility of Ht/Hd, and their release behavior in vitro were investigated. The main driving forces for the complexes of Ht/Hd with glutenin (Glu) or fibrillated glutenin (Glu-F) were hydrogen bonding and hydrophobic forces. However, the enthalpy change of the interaction of the fibrillated glutenin with Ht/Hd was reduced, implying the formation of a more stable Ht/Hd-Glu-F complex. Furthermore, the optimum mass parameter ratio of Ht/Hd:P:Glu-F obtained from orthogonal experiments was 5:4:40 at pH 6. Both Ht and Hd were almost insoluble in water (pH 6), while the formation of the Ht-Glu-F-P and Hd-Glu-F-P complexes increased the solubility of the almost insoluble Ht and Hd to 102.16 ± 4.82 and 220.57 ± 10.82 μg/mL, respectively. After 30 days of storage, the particle size of the emulsions formed by Ht-Glu-F-P and Hd-Glu-F-P complexes increased from 171.90 to 174.84 nm to 231.82 and 219.81 nm, respectively, which had better storage stability than those formed from Ht/Hd-Glu-P complexes. The increased intermolecular repulsion caused by fibrillation modification improved the stability of the delivery system. These results provide a unique method for improving the aqueous dispersion and storage stability of Ht/Hd.
研究领域:
食品科学
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生物化学
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Haihua Jiang,Xing Hu,Junhui Pan,Deming Gong,Guowen Zhang
This research aimed to investigate the changes in the structure and functional properties of glutenin (Glu) induced by hesperetin (Ht) or hesperidin (Hd). Both Ht and Hd quenched the fluorescence of Glu through a static process and formed a Ht/Hd–Glu complex in one binding site. The binding constants between Glu and Ht and Hd were 4.20 × 10 4 L mol −1 and 3.59 × 10 4 L mol −1 at 25 °C, respectively. The computer simulations showed their bindings were dominated by hydrogen bonds and hydrophobic forces, and also provided the changes in particle motion and macroscopic properties of Glu induced by Ht/Hd. The presence of Ht/Hd promoted the exchange of sulfhydryl and disulfide bonds, resulting in the structural changes of Glu and the free sulfhydryl groups more exposed. Meanwhile, the increase in β-sheet content of Glu might cause the contraction of the carbon chain, thereby increasing the surface hydrophobicity. Due to the increase in rigid clusters and the decrease in flexibility, thermal stability of Glu was enhanced. Moreover, the emulsifying activity of Glu was significantly improved. This research lays the foundation for the application of Ht/Hd and Glu in healthy food or the optimization of Glu-based products.
研究领域:
食品科学
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生物化学
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产品关联
DOI
高影响因子
BIOTECHNOLOGY LETTERS
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2024
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IF 2
Zhang Fei,Wang Xue,Pan Lixia,Wang Zhao,Zheng Jianyong
The α- l -rhamnosidase ( rha 1) gene was homologously expressed in Aspergillus niger strains CCTCC 206047 and CCTCC 206047Δ pyr G, using hygromycin B and auxotrophic as selection markers. The engineered A. niger strains RHA001-1 and RHA003-1 were screened, yielding α- l -rhamnosidase activities of 20.81 ± 0.56 U/mL and 15.35 ± 0.87 U/mL, respectively. The copy numbers of the rha 1 gene in strains RHA001-1 and RHA003-1 were found to be 18 and 14, respectively. Correlation analysis between copy number and enzyme activity in the A. niger strains revealed that α- l -rhamnosidase activity increased with the copy number of the rha 1 gene. Recombinant α- l -rhamnosidase was utilized for the enzymatic debittering of Ougan juice, and its process conditions were optimized. Furthermore, the primary bitter substance neohesperidin (2.22 g/L) in Ougan juice was converted into hesperetin 7-O-glucoside (1.47 g/L) and hesperidin (0.143 g/L). This study presents a novel approach for the production of food-grade α- l -rhamnosidase and establishes a technical foundation for its application in the beverage industry.
研究领域:
生物技术
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食品科学
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酶学
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产品关联
DOI
PubMed
高影响因子
Materials
·
2024
·Vol. 17
·期 16
·3919
·
IF 3.1
Yuhong Jiao,Lu Li,Jinlong Ge,Yanfang Tai,Hui Han
In this work, we demonstrated a novel, sensitive and effective fluorescent naringin (NRG) and naringenin (NRGe) detection method using polymethionine nanoparticles (PMNPs) as a fluorescent nanoprobe. The PMNPs were first synthesized by autopolymerization of methionine at 90 °C when trace copper ions existed. The as-prepared PMNPs were thoroughly characterized by transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FT-IR), gel permeation chromatograph (GPC), nuclear magnetic resonance spectroscopy (NMR), transient and steady-state fluorescence and UV–Vis absorption spectroscopy. The quenching mechanism was attributed to the inner filter effect (IFE). Moreover, the developed assay was used successfully to detect NRG and NRGe in real samples of citrus fruits, illustrating that this detection method has great potential application in the field of citrus fruits analysis.
研究领域:
分析化学
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食品科学
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植物化学
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DOI
PubMed
高影响因子
Foods
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2021
·Vol. 10
·期 5
·963
·
IF 4.35
Mianhong Chen,Ruyi Li,Yuanyuan Gao,Yeyu Zheng,Liangkun Liao,Yupo Cao,Jihua Li,Wei Zhou
Naringenin and naringin are a class of hydrophobic polyphenol compounds and both have several biological activities containing antioxidant, anti-inflammatory and anti-tumor properties. Nevertheless, they have low water solubility and bioavailability, which limits their biological activity. In this study, an easy pH-driven method was applied to load naringenin or naringin into nanoliposomes based on the gradual reduction in their water solubility after the pH changed to acidity. Thus, the naringenin or naringin can be embedded into the hydrophobic region within nanoliposomes from the aqueous phase. A series of naringenin/naringin-loaded nanoliposomes with different pH values, lecithin contents and feeding naringenin/naringin concentrations were prepared by microfluidization and a pH-driven method. The naringin-loaded nanoliposome contained some free naringin due to its higher water solubility at lower pH values and had a relatively low encapsulation efficiency. However, the naringenin-loaded nanoliposomes were predominantly nanometric (44.95–104.4 nm), negatively charged (−14.1 to −19.3 mV) and exhibited relatively high encapsulation efficiency (EE = 95.34% for 0.75 mg/mL naringenin within 1%w/vlecithin). Additionally, the naringenin-loaded nanoliposomes still maintained good stability during 31 days of storage at 4 °C. This study may help to develop novel food-grade colloidal delivery systems and apply them to introducing naringenin or other lipophilic polyphenols into foods, supplements or drugs.Keywords:naringenin;naringin;nanoliposomes;pH-driven;stability
研究领域:
药学
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食品科学
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胶体化学
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生物技术
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2019
·
IF 3.714
Xin Huang,Xingran Kou,Li Wang,Ruya Ji,Chaoyang Ma,Hongxin Wang
Tangeretin (TAN) is a biologically functional polymethoxylated flavonoid , extracted from the Citrus Peel (Chenpi). Although 5-demethyltangeretin (5-DT) generated through hydrolysis using a TAN precursor has superior biological function to TAN, its traditional preparation methods are difficult due to environmental hazard and low efficiency processes. In this study, the phosphoric acid (a kind of edible acids) was screened as catalysts of TAN hydrolysis conversion to 5-DT. The main conversion-influencing factors of reaction (material/liquid ratio, temperature and time) were investigated and the antioxidant activity of the modified products was determined by measuring DPPH (Diphenylpicrylbenzoquinone) free radical scavenging capacity and total reducing power. The phosphoric acid/ethanol (v/v) ratio of 7:3, material/liquid (g/L) ratio of 2:1, conversion temperature of 175 °C and conversion time of 2 h were attained as optimal conditions with a 93.71% conversion rate. Further, in vitro antioxidant and anti-lipase experiments confirmed that 5-DT was superior to TAN in total reducing power, scavenging capacity of DPPH free radicals (p < 0.01) and inhibition of lipase activities. Therefore, phosphoric acid is proposed as an alternative catalyst for the modification of TAN. Further, it provided technical support for the industrial production of 5DT and its application in functional foods.
研究领域:
食品科学
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药理学
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化学工程
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生物化学
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产品关联
DOI
高影响因子
Processes
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2022
·Vol. 10
·期 5
·856
·
IF 3.352
Weichao Zhang,Yuwei Wang,Liquan Fan,Xingmei Liu,Weiyan Cao,Honglin Ai,Ziteng Wang,Xijun Liu,Hongge Jia
Efficient and reasonable utilization of waste biomass resources can not only avoid serious waste of material resources, but also solve the problem of environmental pollution. Therefore, the development of efficient and environmentally friendly waste biomass carbonization technology has important practical significance. Here, the activated carbon from orange peel (OAC) is prepared by potassium hydroxide (KOH) activation combined with high-temperature carbonization. The adsorption effects of OAC on three different pollutant aqueous solutions, methylene blue (MB), tetracycline (TC), and fluorescein sodium (NaFL), are examined. The OAC absorbent has excellent adsorption capacity for MB, TC, and NaFL pollutants of 10 mg L−1, with adsorption rates of 99.17%, 73.5%, and 94.24%, respectively. This study provides a new idea for turning waste biomass into treasure and eliminating the hidden danger of environmental pollution.Keywords:activated carbon;adsorption;dye;antibiotic
研究领域:
环境科学
·
材料科学
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DOI
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 14
·5316
·
IF 4.6
Hong-Qi Xia,Wanbing Chen,Diyang Qiu,Jiwu Zeng
Portable and low-cost analytical devices are essential for rapid detection of bioactive substrates in agricultural products. This study presents the first highly integrated microelectrochemical sensor based on pencil graphite for rapid and sensitive detection of hesperidin inCitrus reticulate‘Chachi’ peel. The surface morphology and characterization as well as the electrochemical property of pencil graphite was investigated and discussed. A high electrocatalytic efficiency of hesperidin has been found at used pencil graphite-based microelectrodes. Kinetic analysis was carried out to further understand the electrochemical process of hesperidin at a pencil graphite microelectrode. Consequently, a portable and highly-integrated microelectrochemical sensor exhibits a sensitivity of 0.7251 μA cm−2μM−1and a detection limit as low as 25 nM (S/N = 3), and high selectivity was fabricated. Proposed microelectrochemical sensors were applied to electrochemically determinate the hesperidin content in the extract ofCitrus reticulata“chachi” peel. As a result, the concentration of hesperidin in the actual real sample detected electrochemically with the proposed portable and low-cost microelectrochemical sensors is highly consistent to that obtained with a common chromatographic method, thus indicating the good reliability and that it can be used in practical applications.Keywords:microelectrochemical sensor;hesperidin;Citrus reticulata“chachi”
研究领域:
分析化学
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食品科学
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产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2021
·
IF 7.086
Liangmei Rao,Xinyu Lu,Lulu Xu,Yifu Zhu,Ting Xue,Yu Ge,Zhongshu Duan,Xuemin Duan,Yangping Wen,Jingkun Xu
It is an urgent need to exploit a potentially green, cost efficient and eco-friendly strategy for the utilization of waste kudzu vine. We developed a one-step green preparation of kudzu vine biochar (BC) decorated graphene-like molybdenum selenide (MoSe 2 ) with the oxidase-like activity as intelligent nanozyme sensing platform for voltametric detection of hesperetin (HP) in orange peel using the in-situ hydrothermal synthesis method. The structure and properties of MoSe 2 -BC was characterized, and found that BC significantly improved electrochemical cycle stability, electronic conductivity, electrochemical active area, and electrocatalytic activity of MoSe 2 . The oxidase-like activity of MoSe 2 -BC was confirmed by the oxidization of the colorless substrate 3,3′,5,5′-tetramethylbenzidine (TMB) to form blue products and the change of absorbance intensity of UV–vis absorption spectra . The MoSe 2 -BC exhibited excellent electrochemical sensing performance for the detection of HP in wide linear ranges from 10 nM to 9.5 μM with a low limit of detection of 2 nM using differential pulse voltammetric method. An emerging machine learning technique is used to realize the intelligent sensing of HP, and the performance evaluation of regression analysis was selected to evaluate this technique. This work will provide a guidance for the preparation and application of biochar decorated graphene-like nanomaterials with the oxidase-like activity and the development of intelligent nanozyme sensing platform.
研究领域:
材料科学
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环境科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Haoyue Tang,Yu Zhang,You Wu,Wenyu Wu,Shiying Bi,Junjie Wu,Shitong Yang
Turning agricultural wastes into green adsorbents is a compatible and cost-effective strategy for solid waste disposal and environmental pollution control. Herein, the discarded orange peel (OP) sample was purified via coupled alkali-acid hydrolysis and the obtained adsorbents were applied for eliminating toxic cadmium (Cd(II)) from the liquid phase. Based on the single factor experiments, the optimal Cd(II)-capturing adsorbent (i.e., OP-OH-H-25) was generated under the purification condition of soaking raw OP in 2.0 M NaOH solution at 25 °C followed by an acid hydrolysis treatment with 2.5 M HCl solution at 25 °C. Spectral analyses reflected the efficient elution of hemicellulose/pectin and lignin impurities, the transformation of cellulose type I to cellulose type II, and the exposure of sulfhydryl functional groups. The kinetics removal process of Cd(II) by OP-OH-H-25 reached equilibrium after 0.5 h with a removal efficiency of ∼88%. The maximum Cd(II) sorption capacity of OP-OH-H-25 (i.e., 124.90 mg/g) was higher than a series of previously reported adsorbents. The sorption behavior of Cd(II) onto the prepared OP-OH-H-25 material was mostly interfered with by the coexisting Mg 2+ , Ca 2+ , and heavy metals. The desorption data and spectroscopic analysis jointly pointed to the inner-sphere binding of Cd(II) with OP-OH-H-25 functional groups. Overall, the extracted OP-OH-H-25 sample could act as a promising decontaminant for the remediation of Cd(II)-polluted water environment.
研究领域:
环境科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Shanshan Wang,Tian Lei,Leilei Liu,Zhijian Tan
Hesperidin, also known as vitamin P, is widely studied for its distinctive potential in food and pharmaceutical industries. This work focuses on the research of CO 2 -responsive deep eutectic solvents (DESs) used as recyclable extractants for efficient extraction of hesperidin from Fertile orange peel. Reversible phase transformation of DES solution was achieved by bubbling CO 2 /N 2 . The maximum extraction yield of 22.39 mg/g was obtained for hesperidin under the following conditions: DES-6 (triethanolamine: 4-methoxyphenol = 1:1) used as extractant, water content of 35 wt%, solid–liquid ratio of 1:60 g/mL, extraction temperature of 25 °C, and ultrasonic time of 25 min, which was much higher than that extracted by methanol. Hesperidin of 74.38% was recovered in top phase after CO 2 triggering. Density function theory (DFT) results indicated that hydrogen and π - π bonds were the main factors affecting hesperidin extraction. This study provides a new idea for extracting natural bioactive compounds using recyclable extractants.
研究领域:
食品科学
·
植物化学
·
药物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Drug Design Development and Therapy
·
2020
·
IF 3.216
Hao-Zhen Zheng,Xiao Shen,Ying-Ying He,Xiang-Li Yan,Sheng-Xin Wang,Ai-Ming Yu,Li-Sheng Wang
Objective The aim of our research was to analyze and compare the pharmacokinetics of paeoniflorin, calycosin, calycosin-7-o-β-d-6-glucoside, and 6-gingerol in the blood and brain tissue of normal and cerebral ischemia-reperfusion injury rats by HPLC-MS/MS method.Methods The blood and brain tissue samples of normal and middle cerebral artery occlusion (MCAO) rats were compared. The blood and brain tissue samples were collected by using microdialysis technique. The concentrations of paeoniflorin, calycosin, calycosin-7-o-β-d-6-glucoside, and 6-gingerol in blood and brain tissues were determined by the HPLC-MS/MS internal standard method.Results Compared with the normal group, the model group after the administration of the Huangqi Guizhi Wuwu Decoction showed that Cmax blood, AUC0-t blood, and AUC0-inf blood of paeoniflorin were increased, CLblood, t1/2 brain, and Vbrain of paeoniflorin were decreased; Cmaxblood, AUC0-tblood, AUC0-infblood, and average residence time (MRTbrain) of calycosin-7-o-β-d-6-glucoside were decreased and the CLblood and Cmax brain of calycosin-7-o-β-d-6-glucoside were increased; Cmax blood of calycosin was decreased, Vblood and Vbrain of calycosin were increased; Cmax blood, AUC0-t blood, AUC0-inf blood, and MRTbrain of 6-gingerol were decreased, CLblood of 6-gingerol was increased.Conclusion This method is simple, rapid, and sensitive. It is suitable for the pharmacokinetic study of Huangqi Guizhi Wuwu Decoction in the blood and brain tissue of rats. Cerebral ischemia-reperfusion injury increased the content of paeoniflorin, calycosin, calycosin-7-o-β-d-6-glucoside, and 6-gingerol in the blood, affecting the clearance rate of paeoniflorin in the brain, the detention time of calycosin-7-o-β-d-6-glucoside and the 6-gingerol in the brain. In normal and cerebral ischemia-reperfusion rats, the content of paeoniflorin and 6-gingerol in the blood was higher than that in brain tissue, while the content of calycosin, calycosin-7-o-β-d-6-glucoside in the brain tissue was higher than that in blood, suggesting that calycosin and calycosin-7-o-β-d-6-glucoside have brain targeting properties.
研究领域:
中药学
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药理学
·
神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）