FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Fang Wei,Liyong Luo,Fanwei Sun,Bowen Zhang,Jie Yang,Yan Liu,Liang Zeng
Camellia nanchuanica (NC) black tea has garnered attention for it's appealing sweet taste and aroma . Previously, phloridzin was identified as the key sweet taste compound in NC black tea. To investigate the aroma characteristic of NC black tea, a flavor wheel containing 54 flavor attributes, was developed using 30 samples of NC black tea. Sweet aroma was identified the most prominent characteristic aroma attribute of NC black tea. To further reveal the key sweet aroma compounds, 112 volatile compounds were totally detected using gas chromatography–mass spectrometry. Among them, β -ionone, linalool, phenylacetaldehyde, octanal, and geraniol were the potential key sweet aroma compounds in NC black tea because their relative odorant activity values and odorant activity values were exceeded 1. Notably, when β -ionone, linalool, and geraniol concentrations were 47.17–94.35, 168.02–336.03, 376.79–753.58 μg/L primarily with floral and sweet aroma, significantly enhanced the sweet aroma of NC black tea. Generally, our work not only revealed the flavor characteristics of NC black tea but analyzed its key sweet aroma compounds, which provided a theoretical basis for improving its higher sweet aroma.
研究领域:
茶学
·
食品科学
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DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Dongzhu Huang,Hong Wang,Yuhan Wu,Chenyi Sun,Maoyin Fu,Ying Zhang,Yunqiu Yang,Xiaochun Wan,Yeyun Li,Qi Chen
GABA black tea (GABT), produced by the combination of anaerobic treatment and Chinese traditional black tea processing technology, has a unique flavor and health benefits. However, how to manufacture high-quality GABT efficiently is the major challenge for this area. In this study, a processing improvement of multiple rolling-anaerobic treatment cycle was applied to prepare tailored GABTs, and their comprehensive quality including aroma, taste and color were analyzed. The sensory evaluation results showed that the improvement significantly optimized the overall GABT quality. The accumulation of linalool oxide Ⅰ, citronellol, etc. was the main reason for the superior aroma of tailored GABT, while the dynamic changes of sweet/umami amino acids, catechins and tea pigments played critical roles in the GABT taste and color quality formation during processing. Furthermore, correlation analysis was applied to determine the contribution of various processing steps. These findings provide a novel manufacturing method for high-quality GABT products.
研究领域:
食品科学
·
茶叶加工
·
感官评价
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产品关联
DOI
高影响因子
PLANT PHYSIOLOGY AND BIOCHEMISTRY
·
2020
·
IF 3.72
Guanhua Liu,Mei Yang,Jianyu Fu
Terpenes and their derivatives are vital components of tea aroma. Their constitution and quantity are highly important criteria for the sensory evaluation of teas. Biologically, terpenes are involved in chemical resistance of tea plant against biotic and/or abiotic stresses . The goal of this study is to identify volatile terpenes of tea plants implicated in defense against herbivores and to identify terpene synthase ( TPS ) genes for their biosynthesis . Upon herbivory by tea geometrid ( Ectropis obliqua Prout), tea plants were found to emit two sesquiterpenes, ( E , E )-α-farnesene and ( E )-nerolidol, which were undetectable in intact tea plants. The induced emission of ( E , E )-α-farnesene and ( E )-nerolidol suggests that they function in either direct or indirect defense of tea plants against the tea geometrid. Candidate TPS genes were identified from the transcriptomes of tea plants infested by tea geometrids. Two dedicated sesquiterpene synthases, Cs AFR and Cs NES2, were identified. Cs AFR belongs to the TPS-b clade and can catalyze the formation of ( E , E )-α-farnesene from ( E , E )-FPP. Cs NES2 belongs to the TPS-g clade and can synthesize ( E )-nerolidol using ( E , E )-FPP. The two genes were also both dramatically upregulated by herbivore damage. In summary, we showed that two novel sesquiterpene synthase genes CsAFR and CsNSE2 are inducible by herbivory and responsible for the elevated emission of herbivore-induced ( E , E )-α-farnesene and ( E )-nerolidol, which are implicated in tea plant defense against herbivores.
研究领域:
植物科学
·
分子生物学
·
农学
·
昆虫学
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DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2025
·
IF 6.2
Xia Yin,Yang-bo Xiao,Kuo-fei Wang,Rong-ji Tian,Ying-ying Shen,Han Tang,Run-long Wang,Shu-guang Zhang,Zhong-hua Liu
Shaking is a critical process for enhancing the aroma quality of black tea, though its molecular regulatory mechanisms remain unclear. Through integrated metabolomics and transcriptomics, we found shaking significantly intensifies floral-honey attributes, with a 30–50 % higher market value than conventional black tea.Stir bar sorptive extraction/gas chromatography-mass spectrometry (SBSE/GC-MS) analysis identified 11 key volatile compounds, including methyl jasmonate, jasmine lactone, indole, and nerolidol，as primary contributors to the characteristic aroma profile. The critical biosynthetic window was determined to be the shaking-to-withering transition phase (W8-W15). Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) coupled with transcriptomic analysis revealed that shaking activated lipid hydrolysis mediated by CsPLDα1 and CsGPAT , releasing linoleic acid and α-linolenic acid, which subsequently regulated the synthesis of methyl jasmonate and jasmine lactone through CsLOX-5 , CsOPR3 , and CsJMT . Additionally, the newly discovered CsTSβ1L-1 gene was implicated in potential cooperation with CsTSβ2 for indole biosynthesis regulation, while CsNES-1 and CsNES-5 were established as principal regulators of nerolidol synthesis.These findings collectively elucidate how shaking modulates the lipid, shikimate, and mevalonate pathways to shape black tea aroma, providing concrete molecular targets for industrial optimization of premium floral-honey aroma tea production.
研究领域:
植物学
·
植物科学
·
食品化学
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分子生物学
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农业生物技术
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产品关联
DOI
高影响因子
Food Chemistry-X
·
2021
·
IF 5.182
Qing-Qing Cao,Yan-Qing Fu,Jie-Qiong Wang,Liang Zhang,Fang Wang,Jun-Feng Yin,Yong-Quan Xu
Roasting, a critical process for oolong tea, has been applied to Tieguanyin tea to improve flavor attributes. To investigate the effects of the roasting on the flavor of Tieguanyin , the global metabolomics analysis on the non-volatile and volatile components were proceeded. The weakening of bitterness and astringency, caused by roasting, may be attributed to the decreasing of flavonoids glycosides and procyanidins, whereas the enhancing of sweet aftertaste to the increasing of gallic acid. Besides, l -theanine flavan-3-ols adducts ( N -ehtyl-2-pyrrolidinone substituted flavan-3-ols) increased dramatically at 130 °C compared with 105 °C, with the reduction of l -theanine and flavan-3-ols. Meanwhile, high temperature hampered the volatiles’ diversity and intensity, resulting from the lowering of floral volatiles, i.e., β-ionone, jasmine, and nerolidol, yet the nitrogen-containing heterocyclic compounds increased, e.g., pyrroles and pyrazines. The results can help to comprehensively understand the influences of roasting technology on the flavor and chemistry of oolong tea.
研究领域:
食品科学
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化学
·
代谢组学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
·
2017
·
IF 3.505
Jun Tian,Zhaoqun Lu,Yanzhen Wang,Man Zhang,Xueyan Wang,Xudong Tang,Xue Peng,Hong Zeng
The antifungal activity of Nerol (NEL) against Candida albicans , a pathogenic fungus, has a minimum inhibitory concentration (MIC) of 4.4 mM that causes noteworthy candidacidal activity through an apoptosis-like mechanism. Calcium (Ca 2+ ) levels and reactive oxygen species (ROS) production, which are the major causes of apoptosis, were determined in C. albicans cells treated with NEL and were found to increase, which related to mitochondrial dysfunction and disruption. A series of characteristic changes of apoptosis caused by NEL, including mitochondrial membrane depolarization, cytochrome c (cyt c) release, and metacaspase activation were examined using a flow cytometer and Western blot . The results showed that an increase in intracellular Ca 2+ and ROS led to dramatically decreased mitochondrial membrane potential (MMP); cyt c was also released from the mitochondria to the cytosol. Other early apoptotic features were also observed with the metacaspase activation. Finally, the morphological changes of the cells were observed, including phosphatidylserine (PS) externalization, nuclear condensation, and DNA fragmentation through Annexin V-FITC and PI double staining, TUNEL assay , and DAPI staining. The results supported the hypothesis that NEL was involved in the apoptosis of C. albicans cells not only at the early stages, but also at the late stages. In summary, NEL can trigger mitochondrial dysfunction and disruption via elevation of Ca 2+ and ROS leading to apoptosis in C. albicans . This research on the mechanism of cell death triggered by NEL against C. albicans has important significance for providing a novel treatment of C. albicans infections.
研究领域:
微生物学
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药理学
·
细胞生物学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Dianqi Yang,Xuxu Li,Chenyu Zhang,Hong Liang,Xiaoqiang Ma
Geranyl esters (GEs) are valuable monoterpene esters derived from the esterification of geraniol and various carboxylic acids with a range of unique aromas and properties, making them valuable in perfumery, pharmaceutical, and cosmetic applications. Lipase-mediated esterification is considered to be a sustainable process but is challenged by the lack of a compatible catalytic method in conjunction with a customized microbial biosynthesis of geraniol. In this study, we developed an integrated process to convert glycerol and various carboxylates into GEs. The process includes microbial biosynthesis of geraniol using metabolically engineered Escherichia coli and enzymatic conversion of geraniol into GEs in a fermentation medium-organic biphasic system using an immobilized lipase. The enzymatic step for esterifying the target carboxylates with geraniol achieved >90% conversion under the optimized condition. Coupled with the geraniol from microbial fermentation, 0.59 g/L geranyl butyrate and 1.04 g/L geranyl hexanoate were produced subsequently, demonstrating the feasibility of converting renewable source into monoterpene esters through this integrated process, which bypassed feeding extra geraniol in the conventional lipase-mediated GE synthesis.
研究领域:
生物技术
·
化学工程
·
生物化学
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产品关联
DOI
PubMed
高影响因子
Molecular Catalysis
·
2024
·
IF 3.9
Zehao Li,Wanying Wang,Guoguo Wang,Yingjie Feng,Yan Chen,Zhijian Li,Lulu Zhang,Jinchu Yang
Carvone has been widely used in pharmaceutical, food, cosmetic, and chemical industries due to its pleasant odor with high economic value and pharmacological activities. Carveol dehydrogenase is an enzyme that catalyzes the oxidation of carveol into carvone in the presence of NAD(P) + . In this study, two dehydrogenase genes from Klebsiella sp. O852 ( KlebADH1 and KlebADH2 ) were cloned and expressed in Escherichia coli BL21(DE3), and the purified enzymes were characterized for their biocatalytic properties. KlebADH1 and KlebADH2 displayed optimal catalytic activity at 40 °C and 30 °C, respectively, at pH 9. In addition to carveol, KlebADH1 had the potential to catalyze the oxidation of perillyl alcohol, nerol and geraniol, while borneol and geraniol could serve as potential substrates for KlebADH2. The substrate kinetic parameters of enzymes were performed by changing the concentration of carveol, and the results indicated that KlebADH2 exhibited a high affinity for carveol. Additionally, KlebADH1 and KlebADH2 were confirmed to possess carveol dehydrogenase activity through a double-enzyme-coupled (DEC) biotransformation system. The investigation of the biocatalytic properties of KlebADH1 and KlebADH2 laid a solid foundation for further study of carvone sustainable synthesis.
研究领域:
生物技术
·
酶学
·
化学工程
·
药学
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产品关联
DOI
高影响因子
Materials Advances
·
2024
·
IF 5.2
Yanwei Lai,Dashi Deng,Simin Yuan,Xiaocen Liu,Qifang Lei,Guangzhi Li
Albumin proteins have been extensively explored for functionalized delivery of therapeutics. However, their potential in intravesical delivery has not been thoroughly investigated. In this work, we developed a green method for the synthesis of albumin nanocarriers that can efficiently encapsulate the active monoterpene coumarin UM-15 and assemble with fluorinated chitosan (FCS) to form UM-15@BSA/FCS nanoparticles (NPs). Upon intravesical perfusion, BSA/FCS NPs serving as the nano-vehicles of UM-15 demonstrate exceptional transepithelial delivery, efficient intratumoral diffusion, and high tumor-targeting uptake capacities. These remarkable advantages can be attributed to their notable cascade delivery performances, which involve FCS-driven rearrangement of bladder epithelium tight junctions and the charge reversal effect-induced dissociation behavior of FCS-albumin complexes, as well as the high tumor selectivity mediated by albumin-binding protein (SPARC) and disulfide bonds. Additionally, UM-15 loaded in those nanovehicles demonstrates significant induction of apoptosis and stemness suppression of bladder cancer cells. With above effects acting synergistically, remarkably improved intravesical treatment outcomes are achieved in both mouse orthotopic and patient-derived orthotopic xenograft models, with reduced toxic side effects. Thus, our study introduces a novel therapeutic agent for BCa therapy and presents a promising avenue for the tumor targeted delivery of therapeutics through an intravesical cascade delivery mechanism.
研究领域:
药剂学
·
肿瘤学
·
生物医学工程
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Yujiao Cheng,Tianpeng Xiao,Jiying Zhang,Guowei Hu,Tiangang Lei,Guohua Li,Kelin Du,Qin Long,Yongrui He,Jun Wang,Min Zhang,Linhua Huang
Volatiles in orange juices from three new bud mutation varieties of Longleaf orange were investigated by gas chromatography–mass spectrometry (GC–MS) and multidimensional GC–MS/olfactory (MDGC-MS/O) combined with aroma extract dilution analysis (AEDA). The 103 volatiles (34 odorants) were identified, with concentration increasing as Xiaxiangmicheng (XXMC)>Changyexiangcheng (CYXC)>Zhongganmicheng (ZGMC). The XXMC had the highest number of odorants (31), followed by CYXC (28) and ZGMC (25). C10-hydrocarbons and -alcohols ( d -limonene, linalool, and geraniol), as well as C6-alcohol ((E)-3-hexen-1-ol), exhibited high flavor dilution (FD) values (≥64) and contributed to the fatty, citrusy, floral, and grassy odors in juice. Higher odor activity values of esters conferred stronger fruity note in XXMC, while the lower ester-to-aldehyde ratio in ZGMC failed to mask the undesirable odors from volatile sulfur compounds. Four potential flavor markers were screened to distinguish the three bud mutation varieties.These provide a theoretical basis for citrus variety evaluation and flavor improvement.
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产品关联
DOI
高影响因子
Foods
·
2026
·Vol. 15
·期 3
·583
·
IF 6
Lili He,Liming Dai,Yifan Li,Tianwei Yang,Yun Zhao,Liming Fan,Fawu Su,Zhiying Cai,Min Ye
Botrytis cinereaposes severe postharvest losses in horticultural products, while synthetic fungicides raise food safety concerns. This study developed a GRAS-compliant antifungal strategy using vapor-phaseCymbopogon citratusessential oil (EO). GC-MS revealed citronellal (17.06%) as the dominant bioactive compound. The EO exhibited superior vapor-phase activity againstB. cinerea, with EC50of 14.69 µg/mL (mycelial growth) and MIC of 7.81 µg/mL (spore germination), significantly lower than direct-contact efficacy (p< 0.05). Mechanistic analysis revealed a tripartite mode of action—rapid membrane disintegration (48% electrolyte leakage within 4 h), suppression of ROS defense enzymes (SOD/CAT/POD inhibition > 50%), and disruption of mitochondrial energetics (SDH activity reduced by 58.1%)—which induced irreversible cellular collapse. This multi-target strategy mitigates resistance development, a key limitation of single-mode fungicides. In commercial-scale trials, EO fumigation (125 µg/mL) reduced cherry tomato decay by 81.9–92.6% during 28-day storage, while maintaining firmness (15.9% higher than control) and nutritional quality (titratable acidity (TA) and total sugar content (TSC)). Notably, the vapor-phase EO also exhibited potent inhibitory activity against the spore germination of rubber tree powdery mildew (EC50: 3.19 µg/mL), demonstrating its broad-spectrum antifungal potential. This finding significantly expands the application scope ofC. citratusEO from postharvest preservation to preharvest crop protection. This work provides a scalable, residue-free alternative to synthetic fungicides for industrial postharvest applications.
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Xiuqing Zheng,Jianna Yu,Mengting Huang,Jiaxu Liu,Jiaxing Li,Peixuan Huang,Lu Cheng,Binwang Yang,Haoran Huang,Zhuyue Peng,Wenjie Liu
Gas chromatography–ion mobility spectrometry has been recognized as a rapid, sensitive, portable approach at low cost in food safety analysis, environmental monitoring, and security screening. However, the peak identification remains a major challenge due to the lack of sufficiently comprehensive reference databases. This study successfully developed and validated an integrated gas chromatography–ion mobility spectrometry/mass spectrometry (GC-IMS/MS) platform incorporating Fourier deconvolution (FD) technology and a pinhole IMS-MS interface, aiming to resolve the limitations of low ion utilization efficiency and detector sensitivity imbalance in conventional GC-IMS/MS systems. The implementation of FD multiplexing increased the ion gate duty cycle from <0.4% in conventional beam chopping ion gates to 50%, while a unique interface design substantially enhanced ion transmission efficiency to the mass spectrometer. Experimental results confirmed precise synchronization between IMS drift peaks and MS spectral data for six target volatile organic compounds (ethyl caproate, methyl heptenone, linalool, citronellyl acetate, l (−)-carvone, and nerol) at identical retention times, with an average ion transmission enhancement exceeding 110-fold. The platform also demonstrated satisfactory quantitative performance, with all target compounds exhibiting linearity (R2 > 0.995) across concentration ranges spanning 3 orders of magnitude and achieving detection limits at the ng/mL level. Three complex extracts including tobacco, Da Hong Pao tea, and apricot flavoring were investigated to evaluate the performance of the developed system, and it was demonstrated that the developed system exhibited precise compound identification and high-throughput nontargeted screening capabilities.
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）