DYES AND PIGMENTS
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2020
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IF 4.613
Cheng Zhang,Meilin Liu,Taotao Li,Shengjun Liu,Qian Chen,Jian Zhang,Kui Zhang
In this work, we developed a dual-emission fluorescent carbon dots (CDs) using sodium citrate (SC), triethylenetetramine (TETA) and rose bengal (RB) as raw materials. Under the hydrothermal treatment , the carboxyl group in SC can crosslink with the amino group in TETA to form polymer-like intermediate by amide bond network. The RB molecule, a fluorescein derivative, lost halogen atoms under hydrothermal conditions and integrated with the skeleton structure of the network intermediate. Further dehydration and carbonization resulted in the dual-emission fluorescent CDs. Excited by ultraviolet (UV) light, the CDs exhibited blue and green luminescence centered at 440 nm and 525 nm, respectively. Due to the emission centers of these two colors showed different sensitivity to hypochlorous acid (HClO), we have designed a fluorescence assay to achieve quantitative detection of HClO. Upon addition of different concentrations of HClO to CDs solution in phosphate buffer (pH = 6), the blue emission decreased gradually while the green emission showed only a minimal decrease. By calculating the emission intensity ratio, a good linear relationship was achieved in the range of 0–17 μM, and the limit of detection was as low as 0.75 μM. The results reported here demonstrated a promising way to design organic dye modified CDs for sensing applications.
研究领域:
化学
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分析化学
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材料科学
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产品关联
DOI
高影响因子
Journal of Pharmaceutical Analysis
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2021
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IF 4.769
Yingai Sun,Yuqi Gao,Chunchao Tang,Gaopan Dong,Pei Zhao,Dunquan Peng,Tiantian Wang,Lupei Du,Minyong Li
In recent years, various methods for detecting exogenous and endogenous hypochlorite have been studied, considering its essential role as a biomolecule. However, the existing technologies still pose obstacles such as their invasiveness, high costs, and complicated operation. In the current study, we developed a glow-type chemiluminescent probe, hypochlorite chemiluminescence probe (HCCL)-1, based on the scaffold of Schaap's 1,2-dioxetane luminophores. To better explore the physiological and pathological functions of hypochlorite, we modified the luminophore scaffold of HCCL-1 to develop several probes, including HCCL-2, HCCL-3, and HCCL-4, which amplify the response signal of hypochlorite. By comparing the luminescent intensities of the four probes using the IVIS® system, we determined that HCCL-2 with a limit of detection of 0.166 μM has enhanced sensitivity and selectivity for tracking hypochlorite both in vitro and in vivo.
研究领域:
生物化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2021
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IF 7.46
Hao Wu,Lan-Fang Pang,Na Wei,Xiao-Feng Guo,Hong Wang
We reported N-doped CDs (MTCDs) with excellent nucleus-targeted ability for selectively detecting ClO − . MTCDs can be easily prepared using m -phenylenediamine and 1,2,3-propanetricarboxylic acid as precursors through a solvothermal method. The maximum excitation/emission wavelengths were located at 444/513 nm, with QYs calculated to be 11.4 %. The fluorescence of MTCDs could be selectively quenched by ClO- upon the other ions, reactive oxygen species (ROS) and reactive nitrogen species (RNS). The quenching coefficient showed a good linearity versus C ClO - in the range of 1–100 μM, with detection limit of 0.48 μM. MTCDs were found to have splendid nuclear localization capabilities in MCF-7, Hela and H1299 cells and were successfully applied to image ClO − in cell nucleus and zebrafishes. During the nuclear localization mechanism study, we found a very interesting phenomenon: MTCDs were rare negatively charged CDs targeting nucleus by binding to the groove of DNA through hydrogen bond, which were different with most reported positively charged nucleus-targeted CDs via electrostatic adsorption. This finding proved that negatively charged CDs could also win the nucleus-targeted ability. Besides, this work provided a new strategy for designing nucleus-targeted CDs as well as the research of targeted release of drugs and cancer treatment.
研究领域:
生物化学
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医学影像
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药物设计
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产品关联
DOI
高影响因子
TALANTA
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2020
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IF 5.339
Yuan Jiao,Yating Meng,Wenjing Lu,Yifang Gao,Yang Liu,Xiaojuan Gong,Yang Liu,Shaomin Shuang,Chuan Dong
A facile strategy for the preparation of nitrogen and phosphorus co-doped carbon dots (N, P-CDs) with long-wavelength emission is attractively proposed in one-pot hydrothermal strategy. The resulting N, P-CDs hold exceptional optical features and display excitation wavelength-independent properties with the emission wavelength at 590 nm, which enable it with the satisfactory relative quantum yield (QY) of 15.6% in long-wavelength region. In addition, the proposed N, P-CDs demonstrates specific selectivity towards ClO − over other competitive reactive oxygen species and exhibits rapid fluorescence response time to ClO − . Moreover, the N, P-CDs exhibits low-cytotoxicity and excellent cell membrane permeability for recognizing ClO − in SMMC-7721 cells, which demonstrates their enormous potential in biological system.
研究领域:
材料科学与工程
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生物医学工程
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分析化学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2023
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IF 6.1
Linlin Yang,Lingfei Li,Renyong Liu,Chenggen Xie,Jun Zhao,Wengui Chang,Lijuan Chen,Yehan Yan,Ningning Zhang,Wei Zhang,Bianhua Liu,Liang Yang
Carbon dots (CDs) as a remarkable fluorescent nanomaterial have the advantages of easy preparation, good photostability and high sensitivity. However, the poor aqueous solution stability of carbon dots largely limited their practical application due to the characteristic of easily forming precipitation for long time storage. Here, a kind of cationic fluorescent carbon dots CDs-P(Ph) 3 was designed by introducing a cationic compound, (4-carboxybutyl) triphenyl phosphonium bromide, to construct an electrostatic shell outside the dots. Such electrostatic shell could highly improve carbon dots stability in an aqueous solution to make CDs-P(Ph) 3 stable for long-term storage with negligible aggregation. Meanwhile, the sensitivity of CDs-P(Ph) 3 for hypochlorous acid (HClO) was also enhanced on the basis of the electron-withdrawing effect of cationic substituents on the surface of carbon dots. The limit of detection of CDs-P(Ph) 3 for HClO was as low as ∼0.32 μM. Additionally, the fluorescence of CDs-P(Ph) 3 could be rapid quenched by HClO with a quenching efficiency of more than 80% within 30 s. The excellent stability of CDs-P(Ph) 3 in an aqueous solution made it suitable for on-site detecting HClO in real samples, such as tap, well and lake water. Such designed fluorescent nanomaterial would provide a practical application pathway for optical sensing detection in environmental samples.
研究领域:
纳米材料科学
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环境科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2023
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IF 8.8
Chengda Zhao,Pengxiang Yuan,Dan Wang,Shiyao Li,Huan Yao,Liu-Pan Yang,Li-Li Wang,Fangfang Du
Hypochlorous acid (HClO) is used in food preservation. However, excessive HClO can deteriorate nutritional composition of food, compromise its quality, and potentially induce various diseases. Consequently, the development of multifunctional fluorescent probes for the sensitive and selective detection of HClO is highly anticipated for food safety. In this work, we designed a nanoprobe using N -aminomorpholine (AM)-functionalized bromine-doped carbon dots (Br-CDs-AM) for sensing HClO. This nanoprobe exhibits pH stability, strong resistance to photobleaching, superior long-term photostability (12 weeks), high sensitivity (19.3 nM), and an ultrarapid response (8 s) for detecting HClO residues in food matrices with percentage recovery (96.5 %–108 %) and RSDs less than 5.34 %. In addition, extremely low cytotoxicity and outstanding biocompatibility enable the nanoprobe to be used primarily for lysosome tracking and rapidly visualizing HClO in live cells. Thus, this study provides a new pathway to design unconventional nanoprobes for food safety assessment and subcellular organelle-specific imaging HClO.
研究领域:
分析化学
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食品科学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
TALANTA
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2023
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IF 6.1
Guixin Wang,Kun Chen,Huili Wang,Chunyang Chen,Xuedong Wang
Herein, we designed/developed a mixed fluorescence system with europium metal-organic framework (EDB) and zinc metal-organic framework (ZBNB). At the 270-nm excitation wavelength, the EDB-ZBNB dually emitted at 425 and 615 nm and displayed blue solution under 365-nm UV lamp. When HOCl was fortified, the 425-nm blue emission dropped progressively, while the 615-nm red emission was relatively stable. Upon addition of ClO − , the shortened fluorescence lifetime demonstrated that the quenched 425-nm fluorescence of ZBNB was owing to the occurrence of dynamic quenching effect. Besides, amino groups are protonated in water to form -NH 3+ , which interact with ClO − to form hydrogen bonds , reduce the distance between -NH 3+ and ClO − , produce energy transfer and result in fluorescence quenching . The ratiometric fluoroprobe provided a significant color change from blue to red, making HOCl detection visual and rapid. This fluorescent probe overcome the disadvantage of conventional redox-based fluorescent probes that can be interfered by MnO 4− and other oxidants with stronger oxidizing capacity than free ClO − . Furthermore, a smartphone-based portable sensing platform was developed based on EDB-ZBNB. By using a “Thingidentify” software on smartphone, the sensing platform was used to detect HOCl in waters with a low detection limit of 28.0 nM and the fortified recoveries of 98.87–103.60%. Thus, this study provides a novel and promising platform for the detection of free ClO − in monitoring water quality.
研究领域:
分析化学
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环境科学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2022
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IF 8.008
Suya Zhang,Lulu Ning,Zhihui Song,Xinyue Zhao,Feng Guan,Xiao-Feng Yang,Jianjian Zhang
Early diagnosis of rheumatoid arthritis (RA) is crucial to prevent deterioration and improve the prognosis of disease outcome. However, current clinical diagnostic methods are unable to achieve accurate and early detection of RA. In this work, we designed an activatable organic nanoprobe (ONP-CySe) capable of specific and real-time imaging of ClO– in early RA. ONP-CySe comprises a near-infrared fluorescent selenomorpholine-caged cyanine dye as the sensing component and an amphiphilic triblock copolymer triphenyl phosphine derivative for mitochondria targeting. Our results showed that ONP-CySe successfully detected elevated levels of ClO– in the mitochondria of macrophages with high selectivity, low limit of detection (31.5 nM), excellent photostability, and good biocompatibility. Furthermore, ONP-CySe can also be used to monitor anti-inflammatory responses and efficacies of RA therapeutics, such as selenocysteine and methotrexate, in BALB/c mouse models. Therefore, our research proposes a universal molecular design strategy for the detection of ClO–, which holds potential for early diagnosis and drug screening for RA.
研究领域:
医学诊断
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纳米技术
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药理学
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炎症性疾病
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）