POLYMERS FOR ADVANCED TECHNOLOGIES
·
2018
·Vol. 29
·期 10
·2632-2642
·
IF 2.137
Wen Liu,Xiao Yang,Na Li,Guanghui Xi,Mingshan Wang,Bin Liang,Yakai Feng,Hao Chen,Changcan Shi,Wenzhong Li
Efficient hemorrhage control is of extreme importance to decrease mortality rates arising from traumatic bleeding. However, traditional hemostatic agents are limited to unsatisfied biocompatibility, inconvenient usage, and high cost. To this end, we have devised a novel hemostatic microsphere from chitosan and gelatin by using appropriate amount of genipin as a crosslinker. The performance of these Cs/Gel MPs was evaluated with experiments in vitro and in vivo. Results in vitro showed that the best hemostatic efficacy was achieved by Cs/Gel-0.75 MPs (the mass fraction of genipin in Cs/Gel mixture is 8.6 × 10 −6 ). Skin laceration model indicated that the hemostatic time of Cs/Gel-0.75 MPs was within 12 seconds, which has been significantly shortened in comparison with commercial available products. Moreover, cytocompatibility results displayed that Cs/Gel-0.75 MPs caused no obvious cytotoxicity even at the high concentration of 40 μg/ml. Cs/Gel-0.75 MPs also possess satisfactory fluid absorption ratio and low hemolysis. Therefore, these Cs/Gel-0.75 MPs are promising candidates for further surgical wound hemostatic agent.
研究领域:
生物医学工程
·
材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2018
·
IF 1.911
Junxia Yu,Huantong Su,Shuda Wei,Fangping Chen,Changsheng Liu
Blood coagulates are closely related to calcium ions (coagulation factor IV), and calcium-doped biomaterials have been reported to be effective in hemostasis. However, the effects exerted by calcium on hemostatic agents have not been previously investigated. The aims of this work were to develop calcium-modified oxidized microporous starch (CaOMS) with controllable calcium contents and to explore the relationship between calcium content and hemostatic effects. The results showed that low calcium content promoted coagulation, while high calcium content inhibited coagulation. CaOMS3 with 2.2 mg/g calcium content was optimal because of its excellent water absorption performance that enhanced physical coagulation, the rapid initiation of coagulation cascade reactions, and the enhanced chemical coagulation by RBC aggregation and platelet activation. The synergistic effects of chemical activation and physical absorption endowed CaOMS with the potential to control internal organ bleeding. These results suggested that CaOMS may be a promising hemostatic agent with wide spread applications.
研究领域:
生物医学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Xinhong Zhao,Yunbo Sun,Zhiyun Meng,Zhiyuan Yang,Shan Fan,Tong Ye,Lei Yang,Tong Li,Ruolan Gu,Zhuona Wu,Hui Gan,Guifang Dou,Yongxiu Qi,Shuchen Liu
Effective bleeding control is essential for the reduction of traumatic deaths among civilians and military personnel, particularly for physical visceral and arteriovenous injuries. Materials with good hemostatic properties have recently attracted significant scientific attention. In this study, a novel material of tranexamic acid modified porous starch (TAMPS) was produced through esterification . The structure of the final product was characterized using Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and thermogravimetric analysis . The hemostatic effect of TAMPS was preliminarily analyzed via in vitro clotting time, mouse tail amputation model and liver injury model experiments. Hemostatic effect of TAMPS was found to be significantly better than that of the positive control Quickclean. Through the exploration of related hemostatic mechanisms , TAMPS can promote coagulation via rapid fluid absorption and high erythrocyte aggregation capacity. The in vitro cytotoxicity, acute toxicity, and hemolysis tests revealed that TAMPS is safe and nontoxic and has perfect blood compatibility. Therefore, the TAMPS has a great potential for future clinical application as a rapid and multitarget hemostatic material.
研究领域:
材料科学
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医学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2017
·Vol. 5
·期 20
·3697-3705
·
IF 4.543
Changjiao Yan,Tiehong Yang,Sikai Zhu,Hong Wu
Immediate hemorrhage control without secondary injury is pivotal for saving lives. In this study, polymerized glycidyl methacrylate derivative dextran/acrylic acid (poly(DEX-GMA/AAc)) microgel particles were prepared via emulsion polymerization method as a hemostatic agent. Microgel particles with size distribution of 500–800 nm were chosen because they showed more appropriate characteristics of swelling ratio and gelation time. The results revealed that the microgel particles had excellent swelling ratio of 68.95 g g−1 (w/w), which was 8.4 times that of counterpart clinically used microporous polysaccharide hemospheres, Arista. And poly(DEX-GMA/AAc) showed very short gelation time of 10–13 s. As a result, a gelled film could be formed rapidly after poly(DEX-GMA/AAc) absorbed water in blood when used on wounds, and then staunched bleeding. Poly(DEX-GMA/AAc) microgel particles showed better clotting ability than commercial hemostatic agent Flashclot in vitro. In addition, poly(DEX-GMA/AAc) did not cause exothermic burn when absorbing liquid, which was superior to Flashclot. No obvious toxicity was found in cytotoxicity study and skin irritancy test. Blood loss and hemostasis time were dramatically reduced by poly(DEX-GMA/AAc) microgel particles in hemorrhage models of ear vein, ear artery, liver and femoral artery in rabbits. These results indicated that the poly(DEX-GMA/AAc) microgel particles are a potential hemostatic agent with almost no cytotoxicity and good biocompatibility.
研究领域:
医学材料学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
Biomaterials Science
·
2018
·Vol. 6
·期 12
·3332-3344
·
IF 5.831
Xingtao Chen,Yonggang Yan,Hong Li,Xuehong Wang,Songchao Tang,Quan Li,Jie Wei,Jiacan Su
Absorbable hemostatic agents with a high hemostatic efficacy play an important role in surgical and severely traumatic hemostasis. In the present study, by applying polyelectrolyte assembly, polyelectrolyte complexes (PECs), using carboxymethyl starch (CMS) and chitosan oligosaccharide (COS), with controllable physicochemical properties were prepared and optimized for hemorrhage control. Particle size, zeta potential, morphology and water absorption of the PECs with different CMS/COS ratios were systematically evaluated. The results of in vitro degradation in PBS suggested that CMS/COS PECs were degradable and their degradation rates, which decreased with the increase of the COS content, were suitable for absorbable hemostatic agents. The in vivo hemostatic efficacy of the PECs with 10 wt% COS content (PEC 10), which was evaluated in a rabbit hepatic hemorrhage model, was better than CMS but decreased with the increase of the COS content. The plasma coagulation evaluation revealed that the PECs could significantly activate and accelerate the coagulation cascade through both the intrinsic and extrinsic pathways but could not directly affect the common pathway. CMS/COS PECs also showed antimicrobial activity against S. aureus, which enhanced with the increase of the COS content, but failed against E. coli. Moreover, PEC 10 displayed excellent cytocompatibility with MC3T3-L1 and good tissue compatibility in a rabbit liver model. These findings not only suggest that CMS/COS PECs with a suitable COS content were promising absorbable hemostatic agents for internal use but they are also useful to understand the underlying principles for designing PEC based hemostatic agents.
研究领域:
生物材料
·
止血学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2019
·
IF 6.044
Shangpeng Liu,Zhiqin Zheng,Shuang Wang,Shaojuan Chen,Jianwei Ma,Guosai Liu,Bin Wang,Jiwei Li
In this study, polydopamine (PDA) coated chitosan/calcium pyrophosphate hybrid microflowers (PDA@CS-CaP) were prepared using a facile approach and evaluated as a hemostatic agent. The surface morphology and elements, chemical groups, porous structure, thermostability, zeta potential, as well as surface wettability were systematically characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), N 2 adsorption-desorption, thermogravimetry (TGA) and contact angle apparatus. Due to the synergistic effects of the high hydrophilicity (water contact angel nearly 0), chemical activation (the amino and phenol group of PDA), and the flower-like porous hierarchical structure, the prepared PDA@CS-CaP could induce hemocytes and platelets aggregation, promote the blood clotting and achieve bleeding control in vitro and in vivo. Furthermore, the PDA@CS-CaP had no exothermic adverse effects and exhibited better biocompatibility (no cytotoxicity against fibroblasts cells). Thus, the PDA@CS-CaP were expected to be a promising candidate for a safe and promising hemostatic agent because of its porous and hierarchical structures as well as the active PDA coating.
研究领域:
材料科学
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生物材料
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止血
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 12
·6231-6241
·
IF 2.936
Wenqian Nong,Anran Zhao,Jinrui Wei,Hui Cheng,Xuan Luo,Cuiwu Lin
A series of benzothiazole amide derivatives were synthesized through a facile and efficient method via a nucleophilic acyl substitution reaction between 2-aminobenzothiazole and various cinnamic acid compounds. The obtained products exhibited good thermal stabilities. All compounds were evaluated for their in vitro hemostatic activities using the commercially available standard drug etamsylate as a positive control. The results showed that compound Q2 had a significant partial coagulation activity, reduced capillary permeability at 5, 10 and 50 μmol L−1, activated thrombin activity, and a more potent platelet aggregation activity than the positive control group (etamsylate, up to 1283.9 times in the nanomole range). A molecular modeling study revealed that compound Q2 was a competitive thrombin activator. Therefore, Q2 may be a potential lead for further biological screening and for the generation of drug molecules. Moreover, the structure–activity relationship of the prepared compounds is also discussed herein.
研究领域:
药物化学
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药理学
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止血研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Bioactive Materials
·
2022
·
IF 16.874
Bitao Lu,Enling Hu,Ruiqi Xie,Kun Yu,Fei Lu,Rong Bao,Chenhui Wang,Guangqian Lan,Fangyin Dai
Complex yet lethal wounds with uncontrollable bleeding hinder conventional hemostats from clotting blood at the source or deep sites of injury vasculature, thereby causing massive blood loss and significantly increased mortality. Inspired by the attack action of torpedoes, we synthesized microcluster (MC) colloidosomes equipped with magnetic-mediated navigation and “blast” systems to deliver hemostats into the cavity of vase-type wounds. CaCO 3 /Fe 2 O 3 (CF) microparticles functionalized with Arg-Gly-Asp (RGD) modified polyelectrolyte multilayers were co-assembled with oppositely charged zwitterionic carbon dots (CDs) to form MC colloidosomes, which were loaded with thrombin and protonated tranexamic acid (TXA-NH 3 + ). The composite microparticles moved against blood flow under magnetic mediation and simultaneously disassembled for the burst release of thrombin stimulated by TXA-NH 3 + . The CO 2 bubbles generated during disassembly produced a “blast” that propelled thrombin into the wound cavity. Severe bleeding in a vase-type hemorrhage model in the rabbit liver was rapidly controlled within ∼60 s. Furthermore, in vivo subcutaneous muscle and liver implantation models demonstrated excellent biodegradability of MC colloidosomes. This study is the first to propose a novel strategy based on the principle of torpedoes for transporting hemostats into vase-type wounds to achieve rapid hemostasis, creating a new paradigm for combating trauma treatment.
研究领域:
生物医学工程
·
材料科学
·
止血
·
伤口愈合
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Nano
·
2016
·
IF 13.334
Juan Cheng,Shibin Feng,Songling Han,Xiangjun Zhang,Yidan Chen,Xing Zhou,Ruibing Wang,Xiaohui Li,Houyuan Hu,Jianxiang Zhang
Currently, there is still unmet demand for effective and safe hemostats to control abnormal bleeding in different conditions. With the aim to develop affordable, safe, effective, easily stored, and low-cost hemostats, we developed a series of positively charged nanoparticles by a facile one-pot assembly approach. In this strategy, nanoparticles were formed by cholic-acid-mediated self-assembly of polyethylenimine (PEI). Regardless of different structures of cholic acids and PEIs, well-defined nanoparticles could be successfully formed. The assembly process was dominated by multiple interactions between cholic acid and PEI, including electrostatic, hydrogen bonding, and hydrophobic forces. In vitro studies showed that assembled nanoparticles effectively induced aggregation and activation of platelets. Local application of aqueous solution containing nanoparticles assembled by different cholic acids and PEIs significantly reduced bleeding times in different rodent models including tail transection in mice as well as liver bleeding and femoral artery bleeding in rats or rabbits. Moreover, intravenous (i.v.) injection of this type of positively charged nanoparticles notably prevented bleeding in the femoral artery in rats by targeting the injured site via opsonization of nanoparticles with fibrinogen. By contrast, a control negatively charged nanoparticle showed no hemostatic activity after i.v. delivery. Also, preliminary evaluations in rats revealed a good safety profile after i.v. administration of assembled nanoparticles at a dose 4-fold higher than that used for hemostasis. These results demonstrated that cholic acid/PEI-assembled positive nanoparticles may function as cost-effective and locally applicable or injectable nanohemostats for hemorrhage control in the civilian setting and on the battlefield.
研究领域:
止血学
·
纳米医学
·
材料科学
·
生物材料
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Mi Wu,Shun Mao,Xu Liu,Yunen Liu,Peifang Cong,Jianhua Lv,Huaqin Tian,Yan Zhao
Self-gelling and bioadhesive powders offered promising effective hemostats to suit irregularly shaped, complex and non-compressible wounds for clinical applications. In the current study, chitosan based polyelectrolyte complex coacervate were simply prepared by mixing high concentrations (10 %) of low molecular weight chitosan (CS) and polyacrylic acid (PAA) solutions. Obtained by lyophilization, the physical cross-linked polyelectrolyte complex powders would form a gel within 5 s upon hydration, which demonstrated excellent mechanical properties, significant antibacterial activities, strong and lasting adhesion on wet tissues in physiological environment. In vitro blood clotting assays showed that the CS/PAA powders could remarkably aggregate blood cells and accelerate blood clotting process. As studied by diverse hemorrhage models, including rat tail, liver and heart injuries and dog incision, CS/PAA powders significantly facilitated the decrease of blood loss as well as hemostatic time by creating robust physical barriers and promoting blood clot formation on the bleeding sites. These outstanding properties in terms of easy preparation, rapid self-gelling, strong wet adhesion, effective hemostasis and shape-adaptability endowed CS/PAA polyelectrolyte complex powders with great potential in managing acute hemorrhage of non-compressible trauma.
研究领域:
材料科学
·
生物材料
·
止血
·
组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Zineng Yi,Xuan Zhang,Zhengbao Zha
There are limited fluid hemostatic agents capable of reliably controlling bleeding in wet and high-pressure environments. This study presented a barnacle-derived adhesion hemostatic paste (BAH-paste) exhibiting rapid adhesion to biological tissues and operating independently of the coagulation pathway. Upon contacting with the wound, the lipidic phase would repel blood from the interface while the adhesive phase filled with calcium–aluminum layered double hydroxide (Ca–Al LDH) nanosheets absorb blood to form a tough gel that can adhere to wet tissues, seal blood vessels, and achieve hemostasis. Benefiting from the hindering effect of dislocation movement and crack propagation of Ca–Al LDH nanosheets, the BAH-paste could withstand high burst pressures, and exhibit robust adhesion characterized by interfacial toughness ranging from 150 to 200 J·m −2 , alongside shear and tensile strengths of 40–60 kPa, respectively. The BAH-paste offered considerable fluidity, lower swelling, and efficient hemostatic properties. It demonstrated superior performance over commercial hemostatic agents in facilitating in vivo liver hemostasis in SD rats and New Zealand rabbits. Furthermore, this adhesive hemostatic paste can serve as a drug delivery platform for wound repair, significantly enhancing antimicrobial efficacy. Thus, the adhesive hemostatic paste developed here is anticipated to be effective for controlling deep and irregular bleeding.
研究领域:
生物医学工程
·
材料科学
·
止血材料
·
纳米复合材料
·
伤口愈合
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Yonghe Wang,Yulan Wang,Jing Guo,Fucheng Guan,Xinbin Ji,Minghan Li,Qiang Yang,Jianbin Sun,Shi Feng,Jing Tao
In scenarios of arterial bleeding, common hemostatic agents or dressings often fail due to the loss of active components, high blood loss, and high bleeding pressure. To overcome these challenges, we have developed a novel bio-based hemostatic membrane capable of rapid hemostasis under high pressure. A biomass hemostatic membrane with rapid hemostatic function under high pressure was prepared based on polyelectrolyte electrostatic self-assembly and a “concrete reinforcement” strategy. Hyaluronic acid (HA) was sprayed by airflow to form CSH hydrogel bubbles in the Chitosan (CS) solution. Then CSH hydrogel bubbles were deposited onto cellulose nonwoven (CN) by vacuum filtration, and the CSH composite membrane was obtained after drying. The hemostatic effect of several CSH composite membrane was evaluated in the analysis of venous bleeding in vitro (1.5 KPa). Among them, CS 1 HA 0.4 @CN had a small amount of blood loss, but the amount of blood loss was only about 0.29 g, the other samples did not show any blood loss. In vitro arterial bleeding analysis, CS 1 HA 0.2 @CN showed significant hemostasis in just 20 s and successfully stabilized the blood flow at 0.002 mL/s within 40 s. Despite being subjected to a high pressure of 96.235 KPa, the CSH composite membrane effectively maintains its hemostatic capability. Consequently, it is viewed as a promising solution for emergency arterial hemostasis.
研究领域:
生物材料
·
止血
·
组织工程
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）