PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2024
·
IF 4.2
Pan-Pan Guo,Xi-Bin Yang,Hong Yang,Cao Zhou,Gui-Yun Long,Dao-Chao Jin
β -N-Acetylglucosaminidases and/or β - N -acetylhexosaminidases (NAGs / Hexes) are crucial exonucleases, playing a crucial role in the insect molting process. SfHex3 and SfHex4 contain conserved catalytic domains of GH20 and GH20b, clustered into NAG2 and NAG1 group, respectively. SfHex3 and SfHex4 were mainly highly expressed in the 4th–5th instar nymphs, as well as in the integument and ovary. The expression level of SfHex3 gradually decreased in male and female adults, and SfHex4 on the first day of female was significantly higher than that on the first day of male. In addition, RNA interference (RNAi) results demonstrated that the downregulation of SfHex3 and SfHex4 expression in 5th-instar nymphs resulted in failed molting, and a high mortality. Furthermore, after RNAi with SfHex3 and SfHex4 , the transcript levels on key genes of the chitin metabolism pathway ( SfCHS1 , SfCHS1a , SfCHS1b , SfTRE1 , SfTRE2 , SfCht5 , and SfCht7 ) were significantly decreased compared to the control group. Meanwhile the expression levels of SfHex3 and SfHex4 were up-regulated after 6 h and 12 h of 20E treatment. And the transcription levels of SfHex3 and SfHex4 were significantly inhibited at nitenpyram LC 20 , LC 50 , and LC 90 after 96 h of treatment, in 3rd nymphs of Sogatella furcifera . In conclusion, SfHex3 and SfHex4 play important roles in the nymphal development of S. furcifera , contributing to the molting process from nymph to adult. This study not only enhances our understanding of the nitenpyram in pest control, but also provides a foundation for the development of new control strategies using RNAi to targeting SfHex3 and SfHex4 .
研究领域:
昆虫学
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分子生物学
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DOI
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2024
·
IF 10.8
Guixin Han,Ren Kong,Chunsheng Liu,Kai Huang,Qiaolin Xu,Jian Wu,Jiamin Fei,Hui Zhang,Guanyong Su,Robert J. Letcher,Jianbo Shi,Jason R. Rohr
Pesticides have been frequently detected in global freshwater ecosystems, but attempts to document changes in population dynamics of organisms upon exposure to pesticides, establish a causal relationship between exposure and population effects, and identify the key toxic events within individuals under natural field conditions remain rare. Here, we used a field survey, a reciprocal cross-transplant experiment, and a laboratory toxicity experiment to build a compelling case that exposure to the insecticide chlorpyrifos was responsible for differences in snail (Bellamya aeruginosa) densities in eastern (ELL) and western basins of Liangzi Lake in China. Our field survey and reciprocal cross-transplant experiment revealed significant differences in snail densities, juvenile percentage, survival, and relative telomere length (RTL) in the two basins. The insecticide chlorpyrifos detected in snail tissues was negatively correlated with snail densities, the percentage of juvenile snails, and RTL and had an extremely high risk quotient in ELL. In the laboratory experiment, tissue concentrations of chlorpyrifos detected in ELL were associated with reduced RTL and increased juvenile mortality in B. aeruginosa. These results support the hypothesis that chlorpyrifos exposure in ELL reduced the density of snails by reducing juvenile survival and, consequently, recruitment to the adult population.
研究领域:
生态学
·
毒理学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
CLINICA CHIMICA ACTA
·
2023
·
IF 5
Yueyuan Zhang,Sha Xu,Jianxin Xu,Fuchao Xu,Geng Lu,Jiawei Zhou,Shuangshuang Gu,Jun Wang
Background Early evaluation of the severity of sepsis and estimation of its prognosis remains one of the main challenges in current therapeutic strategies. This study aimed to evaluate the prognostic value of plasma 7-ketocholesterol (7-KC) in sepsis. Methods We retrospectively measured by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) the plasma 7-KC concentration in 176 patients with sepsis and 90 healthy volunteers. A multivariate Cox proportional hazard model was introduced to identify independent factors, including plasma 7-KC and clinical features, for the 28-day mortality of sepsis, and a nomogram for predicting the 28-day mortality of sepsis was established. Decision curve analysis (DCA) was performed to assess the prediction model of death risk of sepsis. Results The area under the curve (AUC) of plasma 7-KC in diagnosing sepsis was 0.899 (95% CI = 0.862–0.935, P < 0.001), while it was 0.830 (95% CI = 0.764–0.894, P < 0.001) in diagnosing septic shock. The AUCs of plasma 7-KC in predicting the survival of sepsis patients in the training cohort and the test cohort were 0.770 (95% CI = 0.692–0.848, P < 0.05) and 0.869 (95% CI = 0.763–0.974, P < 0.05), respectively. In addition, high plasma 7-KC expression predicts poor prognosis in sepsis. Then, 7-KC and platelet count were identified as the two factors with significant differences by a multivariate Cox proportional hazard model, and the 28-day mortality probability ranged from 0.002 to 0.985 and was assessed using a nomogram. DCA results revealed that the combination of plasma 7-KC and platelet count showed the best prognostic efficiency of the risk threshold compared to a single factor in both the training cohort and test cohort. Conclusions Collectively, the elevated plasma 7-KC level is an indicator of sepsis and was identified as a prognostic indicator for sepsis patients, providing a landscape for predicting survival in early sepsis with potential clinical utility.
研究领域:
临床医学
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生物化学
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重症医学
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产品关联
DOI
PubMed
高影响因子
Acta Cardiologica Sinica
·
2018
·
IF 0.927
Li-Tan Yang,Yao-Mao Chang,Tsung-Han Hsieh,Wen-Hsuan Hou,Chung-Yi Li
Background We conducted a time-series analysis of daily ambient temperature and all-cause, cardiovascular, and respiratory disease mortality in Taiwan, which is generally neither extremely hot nor cold. Methods Data on all-cause daily mortality rates (excluding accidents, suicide, and homicide), and mortality rates due to respiratory and cardiovascular diseases between 2008 and 2010 were obtained from the Taiwan Death Registry. The daily temperature for that period was averaged from 33 monitoring stations nationwide. A generalized least square model was constructed to assess the relationship between the time-series trends of temperature and mortality, and the cross-correlation function was used to determine the possible time lag for the effect of temperature on mortality. Results As the average temperature increased, the daily all-cause (β = -0.006) and respiratory disease (β = -0.012) mortality rates decreased. On the other hand, an inverse relationship (β = -0.028) between average daily temperature and cardiovascular disease mortality was observed only for a temperature between 12.91 °C and 26.36 °C. The time lag for all-cause and cardiovascular disease mortality was similar at 4-6 days, while the lag for respiratory disease was longer at 13-16 days. Conclusions We found inverse associations between average temperature and all-cause and respiratory mortality. An inverse association between temperature and cardiovascular disease mortality was observed only from 12.91 °C to 26.36 °C.
研究领域:
流行病学
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环境医学
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公共卫生
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心血管病学
·
呼吸病学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL IMMUNOPHARMACOLOGY
·
2011
·
IF 2.325
Jingjing Kang,Yushun Zhang,Xiang Cao,Jie Fan,Guilan Li,Qi Wang,Ying Diao,Zhihui Zhao,Lan Luo,Zhimin Yin
As a natural alkaloid extracted from Amaryllidaceae , lycorine shows various biological effects on tumor cells. Here we show that lycorine dose-dependently inhibited the LPS-induced up-regulation of nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein level in RAW264.7 cells. Besides, it also inhibited NO, PGE 2 , TNF-α and IL-6 release from LPS-treated RAW264.7 cells. RT-PCR experiments showed that lycorine suppressed LPS-induced iNOS but not COX-2 gene expression. Moreover, lycorine decreased LPS-induced mortality in mice. Mechanistically, LPS-induced activation of P38 and STATs pathways was suppressed significantly by lycorine. In addition, lycorine did not interfere with the phosphorylation of ERK1/2, JNK1/2 and NF-κB pathways. In conclusion, lycorine inhibits LPS-induced production of pro-inflammatory mediators and increases the survival rate of mice after LPS challenge, suggesting that lycorine could play an anti-inflammatory role in response to LPS.
研究领域:
药理学
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免疫学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FREE RADICAL BIOLOGY AND MEDICINE
·
2023
·
IF 7.4
Daohui Gong,Xueping Liu,Pengfei Wu,Yue Chen,Yuhang Xu,Zhan Gao,Hang Qian,Guansong Wang,Binfeng He
Macrophage dysfunction is a significant contributor to more than 70 % of sepsis-related deaths, specifically secondary bacterial infections, during the immunosuppression stage of sepsis. Nevertheless, the role of Rab26 in this context remains unclear. In this study, we observed a substantial decrease in Rab26 expression in macrophages during the immunosuppressive phase of sepsis, which was also found to be suppressed by high extracellular levels of HMGB1 . During the progression of sepsis, Rab26 deficiency promotes a polarization shift from the M1 to the M2-like phenotype in macrophages, rendering them susceptible to ferroptosis . Subsequent experimentation has revealed that Rab26 deficiency facilitates the degradation of GPX4, thereby aggravating macrophage ferroptosis through the upregulation of levels of lipid ROS , MDA , and ferrous iron induced by RSL3, a ferroptosis inducer. Additionally, Rab26-deficient mice in the immunosuppressed phase of sepsis exhibit heightened susceptibility to secondary infections, leading to exacerbated lung tissue damage and increased mortality rate. Overall, these findings indicate that Rab26 plays a crucial role in sepsis-induced macrophage immunosuppression by regulating macrophage ferroptosis and polarization. Hence, it represents a potential novel target for sepsis therapy.
研究领域:
免疫学
·
细胞生物学
·
传染病学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 28
·期 46
·65243-65254
·
IF 4.223
Chen Tao,Cao Zhonghong
To explore the relations between the number of accident deaths (NCD) and various factors in construction safety, a multiple linear regression model was established, based on the panel data of economy, construction labor, and the number of accident deaths in 31 provinces (regions) of China from 2009 to 2018. The results show that the average total profits and taxes (TPAT), and the death rate of ten thousand people (DR) are positively correlated with NCD, while the labor productivity (LP) is negatively correlated with it. Under the condition that other factors remain unchanged, NCD increases by 0.02 per a hundred million CNY. However, if DR increases by one ten thousand, NCD will increase by 8.66 on average. On the contrary, when LP increases by a hundred million CNY per ten thousand people, NCD decreases by 0.19. The model also predicts NCD in different provinces and cities from 2019 to 2020 with the method of quadratic exponential smoothing. As well, practice results show that the model is effective and practical.
研究领域:
安全工程
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建筑工程
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应用统计学
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公共安全
·
劳动经济学
✓ 已验证
DOI
高影响因子
BIOCHEMICAL PHARMACOLOGY
·
2023
·
IF 5.8
Runhong Zhou,Jianan Hu,Jingnan Qiu,Shengsheng Lu,Haixing Lin,Ruifeng Huang,Shaofen Zhou,Guoqing Huang,Jian He
Influenza A virus (IAV) is one of the leading causes of respiratory illness and continues to cause pandemics around the world. Against this backdrop, drug resistance poses a challenge to existing antiviral drugs, and hence, there is an urgent need for developing new antiviral drugs. In this study, we obtained a phenolic compound SG -7, a derivative of natural compound 2-hydroxymethyl-1,4-hydroquinone, which exhibits inhibitory activity toward a panel of influenza viruses and has low cellular toxicity. Mechanistic studies have shown that SG -7 exerts its anti-IAV properties by acting on the virus itself and modulating host signaling pathways. Namely, SG -7 targets the HA2 subunit of hemagglutinin (HA) to block the fusion of viral-cellular membranes and inhibits IAV-induced oxidative stress and overexpression of pro-inflammatory factors by activating the Nrf2/HO-1 pathway and reducing NF-κB activation. In addition, SG -7 can enhance type I IFN antiviral response by inducing Nrf2 expression. Importantly, SG-7 showed the ability to inhibit viral replication in the lungs of IAV-infected mice and reduce their mortality. Therefore, SG-7 may be a promising lead compound for anti-influenza drug development .
研究领域:
药理学
·
病毒学
·
分子生物学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2022
·
IF 4.966
Bin Liu,Hui Chen
Bark beetles rely on detoxifying enzymes to resist the defensive oleoresin terpenes of the host tree. Insect cytochrome P450 (CYPs) plays a key role in the detoxification of plant allelochemicals and pesticides. CYP6 family is unique to Insecta, and its biochemical function is basically related to catabolize heterologous substances. In this study, two Dendroctonus armandi CYP6 genes, CYP6DF1 and CYP6DJ2, were characterized. Spatiotemporal expression profiling revealed that CYP6DF1 and CYP6DJ2 expressions were higher in larvae and adult stages of D. armandi than in egg and pupae stages, and that two genes predominantly expressed in brain , midgut, fat body, or Malpighian tubules . Moreover, CYP6DF1 and CYP6DJ2 expressions were significantly induced after exposure to (+)- α -pinene. Importantly, silencing CYP6DF1 and CYP6DJ2 significantly inhibited the CYP activity and increased the mortality in the adults fumigated with (+)- α -pinene. Additionally, piperonyl butoxide exposure to adults also increase the sensitivity after treatment with (+)- α -pinene, which led to a significant reduction of the CYP activity, resulting a significant increase in adult mortality. These results suggest that the CYP6 family plays a key role in determining the susceptibility of D. armandi to (+)- α -pinene, which may have implications for the development of novel therapeutics to control this important pest.
研究领域:
昆虫学
·
生物化学
·
分子生物学
·
害虫管理
✓ 已验证
DOI
PubMed
高影响因子
TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY
·
2021
·
IF 1.437
Shanshan Wang,Hufei Wang,Yanqiu Chen,Jilai Liu,Xiaozhen He,Da Huang,Yuanzi Wu,Yonghai Chen,Zuquan Weng
Human lymphocytes as well as zebrafish embryos were used to study the toxicity of acrylamide, and the intervention effects of the dietary antioxidants (-)-epigallocatechin gallate and curcumin have been examined. The alkaline comet assay revealed that acrylamide (1, 5, 10 mmol/L) had dose-response effects on basic DNA damage and oxidative DNA damage in human lymphocytes. Exposure of zebrafish embryos to acrylamide resulted in decrease of hatching rate, and in increases of mortality and teratogenicity. (-)-Epigallocatechin gallate and curcumin reduced DNA damage and protected zebrafish embryos in the growth and development.
研究领域:
毒理学
·
药理学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
Insect Science
·
2025
·
IF 3
Delong Kong,Ruyue Zhai,Qiling Liang,Hui Guo,Zitong Wang,Changlu Wang,Tongen Ye,Junli Xiao,Lanfeng Wang,Lufei Xie,Tao Ma,Chunsheng Jia,Desen Wang
Desiccant dusts have been shown to be effective materials in the management of bed bugs ( Cimex spp.). Past research primarily focused on exploring the direct lethal effect of dusts against bed bugs, whereas information on their sublethal effects on bed bugs are limited. In this study, we evaluated the lethal effect of 3 dust products (Johnson's ® Baby powder, Vazor DE powder ™ , and CimeXa™; abbreviated as Baby powder, DE, and CimeXa) against the tropical bed bug, Cimex hemipterus (F.), under laboratory conditions. Results showed that the 3 dust products caused 97%–100% mortality to tropical bed bugs within 14 d, both in no-choice and choice experiments. However, in a brief exposure experiment (crossing a 2.5-cm-wide band), Baby powder exposure caused a significantly lower mortality (23%) than DE (88%) and CimeXa (100%). The more effective dusts (DE and CimeXa) were selected for further evaluation of their sublethal effects on C. hemipterus . DE and CimeXa caused significantly higher mortality (48%–58%) through horizontal transfer than that of control (6%). Brief exposure to DE and CimeXa dusts did not affect tropical bed bug activity and their response to CO 2 . However, CimeXa-exposed tropical bed bugs exhibited reduced feeding and lowered survival rate after feeding. Moreover, both DE and CimeXa dust bands showed strong barrier effects on the crawling of tropical bed bugs. Our results indicate that both DE and CimeXa have great potential for preventing C. hemipterus from reaching protected areas and for controlling C. hemipterus .
研究领域:
昆虫学
·
害虫管理
·
杀虫剂毒理学
·
应用生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Yaqiong Liu,Fang Wu,Ming Zhang,Yutong Jin,Xinlei Yuan,Yanling Hao,Lishui Chen,Bing Fang
Human milk oligosaccharides could prevent pathogenic bacterial infections in neonates; however, direct in vivo anti-infection evidence was still lacking. Here, we systematically evaluated the effects of 2′-fucosyllactose (2′-FL) and 3′-sialyllactose (3′-SL) on the structural development and functional maturation in neonates and their defense against enteroaggregative Escherichia coli infection. It was found that supplementation with 2′-FL and 3′-SL improved the resistance of weaned mice to enteroaggregative E. coli. The mechanism related to the promotion of 2′-FL and 3′-SL in the maturation of intestinal mucosal immunity by promoting stem cell differentiation, mucus layer integrity, and tight junction formation. 2′-FL and 3′-SL significantly increased the ratio of Th1 and Treg cells in the lamina propria, contents of short-chain fatty acids, as well as the serum content of IgA. This study lays a theoretical basis for the application of 2′-FL and 3′-SL in infant formula, as well as the development of intestinal health products.
研究领域:
儿科
·
免疫学
·
微生物学
·
营养科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）