JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Jialing Min,Zitong Wang,Chenglin Liang,Wenjie Li,Jie Shao,Kunrui Zhu,Lu Zhou,Jing Cheng,Shihong Luo,Lehan Yu,Yudong Wu,Mingyong Xie,Xiaojuan Hu
To date, there has been limited information on phytoestrogen (PE) exposure and metabolism in breastfed infants. In the present work, 50 sample pairs of Chinese breastfed infants’ urine and the corresponding breast milk were collected. The contents of the relevant PE metabolites in the biosamples were detected via liquid chromatography–tandem mass spectrometry. The correlations between the PE metabolite contents in breastfed infants’ urine and those in the corresponding breast milk were analyzed. The average concentrations of total PE metabolites in breast milk and urine were 0.27 and 0.23 nmol/mL, respectively. Genistein and enterolactone levels in the infant urine were positively correlated with their concentrations in the corresponding breast milk samples, which implies that urine excretion can be utilized as a noninvasive parameter for precise genistein and enterolactone intake assessment. Additionally, the efficiency of PE urine excretion showed significant differences across infants with different ages, genders, and durations of pregnancy.
研究领域:
营养学
·
分析化学
·
儿科医学
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DOI
PubMed
高影响因子
Nutrients
·
2024
·Vol. 16
·期 19
·3351
·
IF 4.8
Haiyan Wang,Zhenyu Yang,Shuxia Wang,Huwei Wu,Xuehong Pang,Yichun Hu,Xiaoguang Yang
Objectives: This study sought to understand the levels of vitamin K in the mature milk of Chinese lactating mothers, thereby providing a foundation for the development of appropriate intake (AI) of vitamin K for infants aged 0–5 months. Methods: Five hundred healthy lactating mothers were selected from the mature milk bank established by the Chinese Maternal and Infant Nutrition and Health Cohort by using a simple random sample procedure. Relevant information about lactating mothers and their infants was obtained by a questionnaire survey. Vitamin K1 and vitamin K2 (MK-4 and MK-7) in mature milk were determined by liquid chromatography–tandem mass spectrometry. Results: The total concentration of vitamin K in mature milk was 4.50 (2.85–6.33) ng/mL. The concentrations of vitamin K1, vitamin K2, MK-4, and MK-7 were 2.81 (1.66–4.39) ng/mL, 1.37 (0.75–2.11) ng/mL, 1.20 (0.58–1.97) ng/mL, and 0.13 (0.08–0.19) ng/mL, respectively. The concentration of vitamin K1 was highest and the concentration of MK-7 was lowest. The concentrations of vitamin K2 and MK-4 in mature milk from the south were significantly higher than those in mature milk from the north. The total vitamin K, vitamin K2, and MK-4 concentrations in mature milk of lactating mothers residing in urban areas were higher than those in rural areas. There was a tendency for the concentration of total vitamin K and vitamin K1 to increase with the mother’s age. Moreover, the concentration of MK-4 in mature milk was highest in summer, followed by spring and winter. The levels of vitamin K1 and MK-4 in mature milk were found to be affected by lactation stage; they were highest at 91–120 days and lowest at 31–60 days. Conclusions: Based on the concentration of vitamin K in mature milk found in this study, it is recommended that the appropriate intake of VK for Chinese infants aged 0–5 months is 3.49 μg/d (2.18 μg/d for VK1 and 1.06 μg/d for VK2).
研究领域:
营养学
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儿科医学
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生物化学
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产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Yue Pan,Lu Liu,Songfan Tian,Xiaodong Li,Muhammad Hussain,Chunmei Li,Lihong Zhang,Qiumei Zhang,Youbin Leng,Shilong Jiang,Shuyan Liang
In this study, we analyzed and compared the fat globule interfacial compositions and structures in human milk and three types of infant formulas (IF1: supplied with MFGM , IF2: without MFGM and soy lecithin , IF3: supplied with soy lecithin). The results suggested that the interfacial protein composition of IF1 was comparatively closer to human milk, but still lacked certain bioactive MFGM proteins including XO, ADPH and PAS6/7. Considering the interfacial phospholipid , we observed 23, 31, and 29 phospholipid species that could be used to distinguish human milk and infant formulas (IF1, IF2 and IF3). We also found that phosphatidylinositol (PI) and phosphatidylserine (PS), which can absorb lipase, phosphatidylethanolamine (PE), and phosphatidylcholine (PC) and connect functional fatty acids, were lacking in infant formulas. Moreover, the infant formulas had a smaller average particle size of 0.38 μm and a thicker interfacial layer that interacted with the casein micelles. Even if IF1 included MFGM, fat globules structures like those found in human milk did not form, and the majority of the MFGM was still in the aqueous phase in free form. Overall, the comprehensive analysis in this study could be used to simulate or mimic human milk lipids at the supramolecular level.
研究领域:
营养学
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食品科学
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生物化学
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DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Shihuan Li,Jinhui Li,Senwei Feng,Linlin Bian,Zhuoting Liu,Yunfang Ping,Xu Wang,Ann Van Schepdael
The presence of fatty acid esters of 3-monochloropropane-1,2-diol (3-MCPDE), 2-monochloropropane-1,3-diol (2-MCPDE) and glycidyl (GE) in foods is a potential concern due to their toxicity, especially for infants. This study proposed a rapid, sensitive and environmentally friendly method for simultaneous determination of the 3-/2-MCPDE and GE in breast milk and infant formula using headspace solid-phase microextraction (SPME) and on-fiber derivatization combined with gas chromatography-tandem mass spectrometry (GC-MS/MS). The approach proved high sensitivity with detection limits of 0.10 μg/L and 0.15 μg/kg for MCPDEs in breast milk and infant formula, respectively. Mean recoveries were obtained ranging from 79.9 to 111.2% for breast milk and from 82.1 to 106.7% for infant formula, with relative standard deviation (RSD) of less than 9.5%. According to the levels of 3-MCPDE and GE determined in 30 breast milk and 23 infant formulas, the exposure to 3-MCPDE and GE may pose a potential health risk for infants from birth to <12 months fed by breast milk and infants from birth to 6 months fed by infant formula.
研究领域:
食品安全
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分析化学
·
毒理学
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产品关联
DOI
高影响因子
Nutrients
·
2022
·Vol. 14
·期 24
·5397
·
IF 6.706
Yapeng Li,Lei Ren,Yunyi Wang,Jinxing Li,Qingqing Zhou,Chenrui Peng,Yuchen Li,Ruyue Cheng,Fang He,Xi Shen
Evidence shows that breast milk microbiota and an infant’s gut microbiota are related. This study aimed to compare the effects of breast milk microbiota on the construction and colonization of gut microbiota in newborns. In this study, 23 healthy infants were selected and divided into a breastfeeding group (13) and a mixed feeding group (10) based on the feeding method within one month of age. Infant fecal and breast milk samples were collected on the day of birth (0 day) and 30 days after birth (30 days) for 16S rRNA second-generation sequencing and SCFA detection. The results showed thatFirmicutesandActinobacteriotaon day 0 andFirmicutesandProteobacteriaon 30 d dominated breast milk gut microbiota. There were correlations between the breast milk microbiota and the infant gut microbiota in each group (p< 0.05). Additionally, breast milk microbiota correlated more significantly with infants’ SCFAs in the breastfeeding group than in the mixed feeding group. This study showed that breast milk microbiota partially influences the construction of infant gut microbiota, with some key strains having a crucial influence, such asLactobacillus,Bifidobacterium, andEnterobacter. However, the effect of breast milk microbiota on infant gut microbiota is not through direct strain transmission but has been indirectly influenced, which may be related to the cross-feeding effect mediated by SCFAs.Keywords:infant;gut microbiota;breastfeeding;SCFAs
研究领域:
微生物学
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儿科学
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DOI
PubMed
高影响因子
ANALYTICAL LETTERS
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2020
·
IF 1.467
Lanlan Wei,Xiaoting Lu,Xuejun Kang,Yuan Song
Exposure to heavy metals may affect a vast array of biochemical and nutritional processes in the human body. The toxic manifestations of these metals are caused primarily due to the imbalance between pro-oxidant and antioxidant homeostasis which is termed as oxidative stress. A high-performance liquid chromatography–chemiluminescence (HPLC-CL) method was developed for the simultaneous determination of two antioxidants, glutathione and cysteine, in human breast milk. The HPLC system included a C18 analytical column with a mobile phase composed of 1.0% methanol. The aminothiols were eluted from the C18 column, mixed with formaldehyde and potassium permanganate solutions, and subsequently monitored by a chemiluminescence detector. The new method was validated by monitoring glutathione and cysteine in human breast milk of volunteers from the Gusu and Industrial Districts of Suzhou, China. Four heavy metals in atmospheric particulate matter collected from the two districts were also determined by inductively coupled plasma–mass spectrometry (ICP-MS). The results demonstrated that the concentrations of cysteine and glutathione were significantly (p < 0.05) lower in human breast milk from the subjects living in the Gusu region of Suzhou, Jiangsu Province, China compared to an industrial district. These results were inversely correlated with the concentration levels of the toxic metals in collected atmospheric particulate matter. The decrease in the glutathione and cysteine concentrations in human breast milk appeared to be due to oxidative stress in the breast-feeding mothers.
研究领域:
分析化学
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环境科学
·
医学
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DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Bochen Wei,Fengxia Zhang,Mengyu Wang,Shaobo Li,Muhammad Qamer Abbas,Yuping Wan,Zhenxia Du
To reduce the differences in phospholipids (PLs) of human milk and infant formula, PL-rich raw materials and finished milk powder from the infant formula production line were collected and the phospholipids profiles of raw materials were systematically analyzed by LC-Q-TOF based on phospholipid omics. 108 phospholipids were detected in infant formula, 73 of which were common to human milk, the content of the different phospholipid molecules varied. For the production of raw materials, whey protein concentrate and arachidonic acid powder have the most similar structure to human milk. By varying the ratio of each ingredient, formula can be made closer to human milk. The particle swarm optimization (PSO) algorithm was applied for the optimization of the ratio of raw materials to closely mimic the human milk. Based on the optimized ratio, the optimized formulas were prepared. PCA analysis proved that the optimized formulas were highly effective in mimicking human milk.
研究领域:
营养学
·
生物化学
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产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2023
·
IF 11.2
Xiaoqin Wang,Wenqing Li,Zhenhua Li,Tianjiao Han,Jinqiao Rong,Jiangbo Fan,Linjuan Huang,Yu Lu,Zhongfu Wang
Human milk glycans are complex carbohydrates , which play a pivotal role in infant health and neonatal development . Maternal secretor status is known to affect free oligosaccharides in milk. Here, the milk N -glycome of secretor (Se+) and nonsecretor (Se−) individuals was qualitatively and quantitatively analyzed by hydrophilic interaction chromatography-electrospray ionization-tandem mass spectrometry. The total glycosylation , fucosylation , and sialylation of N -glycans was three times higher in the Se+ group compared to the Se− group ( p < 0.001) per equal volume of milk. Importantly, 52 out of 63 N -glycans—including the eight most abundant ones—differed greatly between Se+ and Se− individuals ( p < 0.05). Moreover, nine N -glycans (H5N3F1, H6N3, H3N5F1, H5N5F1, H5N5F1S1, H5N4F3S1, H6N4F2S1, H6N5F4S1, and H8N7S1) were >10 times more abundant in Se+ milk than in Se− milk. These findings lay a glycomics-basis for designing personalized nutrition supplements for infants.
研究领域:
生物化学
·
儿科医学
·
营养学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Chenyu Jiang,Xinghe Zhang,Jiahui Yu,Ling-zhi Cheong,Xingguo Wang,Wei Wei
Herein, polar lipids, including glycerophospholipid, lysophospholipid, cholesterol (CHOL), sphingomyelin (SM), and ceramide (Cer), in human breast milk were subjected to lipidomic analysis via UPSFC-Q-TOF-MS. A total of 11 lipid classes and 195 molecular species were identified and quantified in human breast milk samples ( n = 126) from three lactational stages across three regions in China. CHOL and SM together account for more than 50 % of the total polar lipids. The total polar lipids, SM, and phosphatidylethanolamine (PE) were ∼ 54.5, ∼17.8, and ∼ 3.5 mg/100 mL, respectively, which both significantly ( P < 0.05) increased from colostrum to later lactational stages. DHA is highly enriched (68 % of the total DHA) in PE molecules. SM and Cer contain high ratios of nervonic acid (24:1 n-9), ∼35 % and ∼ 23 %, respectively. General patterns exist in the variation of polar lipid molecular species in human breast milk across different regions throughout the lactational stages.
研究领域:
营养学
·
脂质组学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
Food & Function
·
2018
·Vol. 9
·期 8
·4234-4245
·
IF 3.289
Long Deng,Qian Zou,Biao Liu,Wenhui Ye,Chengfei Zhuo,Li Chen,Ze-Yuan Deng,Ya-Wei Fan,Jing Li
In order to investigate the fatty acid composition and distribution in colostrum and mature milk, breast milk samples and 24 h food records were obtained from 65 lactating women across three regions in China (Inner Mongolia, North Jiangsu and Guangxi). Fatty acid methyl esters were prepared by standard methods and separated and identified by gas chromatography. Compared with the Chinese breast milk fatty acid data 10 years ago, SFA and trans fatty acids (TFA) in breast milk decreased, while PUFA increased in the present study. Most SFA (C16:0, C15:0, C14:0), cis-C16:1 and several LC-PUFA (C22:5n-3 and C22:6n-3) were predominantly acylated at the sn-2 position. The cis-C17:1 and C22:6n-3 were distributed equally in three positions of triacylglycerol (TAG). Whereas, TFA, conjugated linoleic acids (CLA), cis-C18:1, C18:2n-6, C18:3n-3 and C20:5n-3 were acylated at the sn-1, 3 positions of TAG in human milk. The composition of fatty acids in breast milk was closely related to the diet of lactating mothers. PUFA in breast milk was negatively correlated with the intake of protein, fat and meat, but positively correlated with the intake of carbohydrates. MUFA of human milk was negatively correlated with the intake of dairy products, eggs, fish and shrimp. SFA in breast milk was positively correlated with the maternal intake of meat. In addition, the present results showed that the composition of total fatty acids and sn-2 fatty acids in breast milk varied with the lactation period and the geographical regions in China; however, the regiospecific fatty acid profile seemed not to be affected by the lactation time and regions, although the quantities at each position could be changed.
研究领域:
营养学
·
生物化学
·
母婴健康研究
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Yue Sun,Xueying Zhang,Jiayu Li,Xiaodong Li,Lu Liu,Kouadio Jean Eric-Parfait Kouame
We hypothesized that improving the fat globule structure of infant formulae based on the milk fat globule membrane (MFGM) would regulate metabolites and metabolic pathways, making it more similar to the metabolic properties of human milk. Therefore, we prepared infant formulae with different fat globule structures, including two model infant formulae (F1: fat globules surrounded by MFGM; F2: fat globules surrounded by protein) and one commercial infant formulae containing MFGM, and compared their metabolic differences with those of human milk. The number of differential metabolites between each sample and human milk reached 60 (F1), 132 (F2) and 126 (IF1). Glycerophosphocholines were screened as potential biomarkers to distinguish infant formulae from human milk. Compared with F2 and IF1, the enrichment of amino acid metabolism and lipid metabolism pathways was not significant between F1 and human milk. These results emphasized that F1 had the highest similarity to human milk in metabolic properties.
研究领域:
营养学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2022
·
IF 4.52
Zhuoneng Huang,Keying Liu,Ye Chen,Ye Huang,Xiaohan Wang,Xiaosan Wang
Most infant formula oils differ significantly from human milk fat (HMF) in structure and composition , which is detrimental to the development of infants. In this study, the lipid compositions of Carassius auratus oils (CAOs) from seven provinces of China were systematically analyzed, and the potential of CAO for use in human milk fat substitutes (HMFS) was evaluated. First, the main fatty acids in most CAOs were confirmed to be palmitic, oleic and linoleic acids . It is interesting to find that 57.08–69.27% of palmitic acid tended to be located at the sn -2 position, which showed a consistency with HMF. Afterwards, content of C52 triacylglycerol in CAOs from three regions exceeded 45%. Finally, a HMFS containing CAO from Jiangsu province was prepared by physical blending and it was verified to have a high similarity with HMF after similarity evaluation, which can provide a reference for the application of CAO in infant formulas.
研究领域:
营养学
·
食品科学
·
生物化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）