SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Rongbing Li,Jiangtao Ren,Jinxing Chen,Ling Yang,Zhixuan Yu,Yunhui Li,Dengbin Yu,Youxing Fang,Shaojun Dong
In this study, a high-efficiency colorimetric sensor for water toxicity assessment was successfully constructed by the visual analysis with naked eyes and the high-throughput measurement with a microplate reader. The bioluminescent Photobacterium phoshoreum T3 spp. (T3) and Prussian blue (PB) were used as model bacterium and indicator of the colorimetric sensor, respectively. In order to accurately detect acute toxicities of wastewater, the experimental conditions, concentrations of cells, K 3 [Fe(CN) 6 ] and NaCl , reaction temperature and time, were optimized to be OD 600 = 2, 8 mM, 1.2%, 26.5 °C and 25 min, respectively. Then several samples of simulated wastewater containing Hg 2+ , Cd 2+ , Zn 2+ , 3,5-dichlorophenol (DCP) and formaldehyde (HCHO) were tested. The composite biotoxicity of dry battery leachate and real water sample was also evaluated. The IC 50 values of Hg 2+ , Cd 2+ , Zn 2+ and DCP obtained were 3.60, 1.56, 0.96 and 11.77 mg L −1 , respectively. Moreover, a toxic unit (TU) model was applied to evaluate the binary complex toxicity of Hg 2+ + Cd 2+ , Hg 2+ + Zn 2+ and DCP + Zn 2+ , which showed antagonistic effects. The work demonstrates that the promising high-efficiency sensor can detect quickly and sensitively the single and joint toxicities of multi-pollutants in water, which possesses extensive potentials in field of toxicity detection.
研究领域:
环境科学
·
生物技术
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产品关联
DOI
高影响因子
Toxics
·
2021
·Vol. 9
·期 12
·321
·
IF 4.146
Min Chen,Gaofang Yin,Nanjing Zhao,Tingting Gan,Chun Feng,Mengyuan Gu,Peilong Qi,Zhichao Ding
To achieve rapid and sensitive detection of the toxicity of pollutants in the aquatic environment, a photosynthetic inhibition method with microalgae as the test organism and photosynthetic fluorescence parameters as the test endpoint was proposed. In this study, eight environmental pollutants were selected to act on the tested organism,Chlorella pyrenoidosa, including herbicides (diuron, atrazine), fungicides (fuberidazole), organic chemical raw materials (phenanthrene, phenol, p-benzoquinone), disinfectants (trichloroacetonitrile uric acid), and disinfection by-products (trichloroacetonitrile). The results showed that, in addition to specific PSII inhibitors (diuretic and atrazine), other types of pollutants could also quickly affect the photosynthetic system. The photosynthetic fluorescence parameters (Fv/Fm, Yield, α, and rP) could be used to detect the effects of pollutants on the photosynthetic system. Although the decay rate of the photosynthetic fluorescence parameters corresponding to the different pollutants was different, 1 h could be used as an appropriate toxicity exposure time. Moreover, the lowest respondent concentrations of photosynthetic fluorescence parameters to diuron, atrazine, fuberidazole, phenanthrene, P-benzoquinone, phenol, trichloroacetonitrile uric acid, and trichloroacetonitrile were 2 μg·L−1, 5 μg·L−1, 0.05 mg·L−1, 2 μg·L−1, 1.0 mg·L−1, 0.4 g·L−1, 0.1 mg·L−1, and 2.0 mg·L−1, respectively. Finally, diuron, atrazine, fuberidazole, and phenanthrene were selected for a comparison of their photosynthetic inhibition and growth inhibition. The results suggested that photosynthetic inhibition could overcome the time dependence of growth inhibition and shorten the toxic exposure time from more than 24 h to less than 1 h, or even a few minutes, while, the sensitivity of the toxicity test was not weakened. This study indicates that the photosynthetic inhibition method could be used for rapid detection of the toxicity of water pollutants and that algae fluorescence provides convenient access to toxicity data.Keywords:photosynthetic inhibition;photosynthetic fluorescence parameter;Chlorella pyrenoidosa;toxic water pollutants;rapid detection;biotesting
研究领域:
环境生物学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
ECOLOGICAL INDICATORS
·
2022
·
IF 6.263
Mengyuan Gu,Gaofang Yin,Tingting Gan,Nanjing Zhao,Peilong Qi,Min Chen,Zhichao Ding,Renqing Jia,Jinjing Liu,Mingjun Ma,Ruifang Yang,Li Fang,Wenqing Liu
Pollutants in water seriously threaten the health of human beings and will lead to potential ecological risks to the aquatic environment. Researchers use many chemical, physiological and morphological methods to detect the comprehensive toxicity in water. Among them, the photosynthetic inhibition method is considered a powerful method to detect the comprehensive toxicity in water, takes the chlorophyll fluorescence in the body as a natural probe, and determines the toxicity effect of pollutants by measuring the variation of photosynthetic fluorescence parameters. It has the advantages of fast and convenient measurement and real-time online detection. The widely used photosynthetic fluorescence parameters are the maximal quantum yield of photosystem II (Fv/Fm) and performance index PI ABS . There are the problems that the sensitivity is not high enough, and the substances that can be detected are limited when researchers use Fv/Fm and PI ABS as indicators to detect the comprehensive toxicity in water. So, in this paper, we proposed a construction method of Performance Index of Comprehensive Toxicity Effect (PI CTE ) using multi-parameter fusion based on the chlorophyll a fluorescence rise kinetics curve (OJIP) of algae. The fluorescence information of point J and point I in the curve was integrated. The area surrounded by the curve and the time corresponding to point J and point I were weighted for data items to construct PI CTE . On this basis, Chlorella vulgaris was used as the tested algae species to compare the performance differences of Fv/Fm, PI CTE , PI ABS in the detection of toxic substances under the stress of five common water pollutants: Diuron (DCMU, 3-(3,4-dichlorophenol)-1,1-dimethylurea), Dibromothymoquinone (DBMIB, 2,5-dibromo-3-methyl-6-isopropyl-benzoquinone), Methyl viologen (MV, 1,1ꞌ-dimethyl-4,4ꞌ-bipyridinium-dichloride), Malathion, Carbofuran. The performance differences of Fv/Fm, PI CTE , PI ABS in detecting toxic substances, including lowest detection limit, highest response concentration, stability, and reproducibility, were studied. The results show that: in terms of toxicity detection, the constructed PI CTE was sensitive to all five toxic substances and could detect more kinds of pollutants than Fv/Fm. The lowest detection limit of PI CTE for five toxic substances compared with PI ABS was reduced by 74.43%, 77.01%, 67.30%, 60.69%, and 50.81%, respectively. At the same time, it has a stronger ability to detect toxic substances with high concentration. The stability and reproducibility of PI CTE for detecting the toxicity of DCMU, DBMIB, Malathion, and Carbofuran were all excellent, and all of the relative standard deviation (RSD) was < 5%. This research provides an important basis for constructing a highly sensitive and high-precision detection method for comprehensive toxicity in water.
研究领域:
环境科学
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生物学
✓ 已验证
产品关联
DOI
高影响因子
TOXICOLOGY IN VITRO
·
2015
·
IF 2.903
Lizhen Huang,Shuxiang Zou,Jifeng Deng,Tianming Dai,Jingwei Jiang,Ying Jia,Renke Dai,Shuilin Xie
Drug-induced hepatotoxicity is often caused by cytochrome P450 (CYP)-dependent metabolism of drugs into reactive metabolites. Assessment of hepatotoxicity induced by bioactive compounds is hampered by low CYP expression within in vitro cell lines. To overcome this limitation, piggyBac transposition and monoclonal expansion were used to generate a HepG2 cell line with stable and homogenously high expression of CYP3A4, a prominent CYP isoform. Our studies demonstrate the generated line's constant CYP3A4 expression and activity for over 40 cell passages; to date, it has been in subculture for more than a year without addition of Puromycin. This cell line was utilized to evaluate cytotoxicity of two bioactive (troglitazone and acetaminophen) and two non-bioactive (citrate and galactosamine) compounds by MTT assay. Cell viability significantly decreased upon treatment with bioactive drugs. Moreover, cell lines used in the present study were more sensitive to toxic effects of troglitazone than previously reported. Therefore, this HepG2 cell-based assay system may provide a suitable hepatic model for predicting CYP3A4-mediated hepatotoxicity during preclinical drug development.
研究领域:
药理学
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分子生物学
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毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2016
·Vol. 408
·期 22
·6019-6026
·
IF 3.125
Wang Yanru,Li Peiwu,Zhang Qi,Hu Xiaofeng,Zhang Wen
A toxin-free enzyme-linked immunosorbent assay (ELISA) for aflatoxins was developed using an anti-idiotype nanobody VHH 2–5 as surrogate standard. Anti-idiotype nanobody VHH 2–5 was generated by immunizing an alpaca with anti-aflatoxin monoclonal antibody 1C11. This assay was used to detect aflatoxins in agro-products after a simple extraction with 75 % methanol/H 2 O. Aflatoxin concentration was calculated by a two-step approach: the concentration of VHH 2–5 was first obtained by a four-parameter logistic regression from the detected absorbance value at 450 nm, and then converted to aflatoxin concentration by a linear equation. The assay exhibits a limit of detection (LOD) of 0.015 ng mL −1 , which is better than or comparable with conventional immunoassays. The performance of our VHH surrogate-based ELISA was further validated with a high-performance liquid chromatography (HPLC) method for total aflatoxins determination in 20 naturally contaminated peanut samples, displaying a good correlation ( R
2 = 0.988). In conclusion, the proposed assay represents a first example applying an anti-idiotype VHH antibody as a standard surrogate in ELISA. With the advantages of high stability and ease of production, the VHH antibody-based standard surrogate can be extended in the future to immunoassays for other highly toxic compounds.
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研究领域:
食品安全
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanomaterials
·
2025
·Vol. 15
·期 2
·146
·
IF 4.3
Ying Liu,Zhipeng Zhang,Yuling Wu,Huan Yang,Jiao Qu,Xiaolin Zhu
The disinfection of drinking water is essential for eliminating pathogens and preventing waterborne diseases. However, this process generates various disinfection byproducts (DBPs), which toxicological research indicates can have detrimental effects on living organisms. Moreover, the safety of these DBPs has not been sufficiently assessed, underscoring the need for a comprehensive evaluation of their toxic effects and associated health risks. Compared to traditional methods for studying the toxicity of pollutants, emerging electrochemical sensing technologies offer advantages such as simplicity, speed, and sensitivity, presenting an effective means for toxicity research on pollutants. However, challenges remain in this field, including the need to improve electrode sensitivity and reduce electrode costs. In this study, a pencil graphite electrode (PGE) was modified with carboxylated multi-walled carbon nanotubes (MWCNT-COOH) and nano-iron (III) oxide (α-Fe2O3) to fabricate a low-cost electrode with excellent electrocatalytic performance for cell-active substances. Subsequently, a novel cellular electrochemical sensor was constructed for the sensitive detection of the toxicity of three drinking water DBPs. The half inhibitory concentration (IC50) values of 2-chlorophenylacetonitrile (2-CPAN), 3-chlorophenylacetonitrile (3-CPAN), and 4-chlorophenylacetonitrile (4-CPAN) for HepG2 cells were 660.69, 831.76, and 812.83 µM, respectively. This study provides technical support and scientific evidence for the toxicity detection and safety assessment of emerging contaminants.
研究领域:
环境科学
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分析化学
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毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED PHYCOLOGY
·
2023
·Vol. 35
·期 5
·2219-2239
·
IF 3.3
Gan Tingting,Yin Gaofang,Zhao Nanjing,Tan Xiaoxuan,Wang Ying,Sheng Ruoyu,Ye Ziqi
Microalgal fluorescence kinetics method, as a promising technique for rapidly detecting water toxicity, still lacks a consistent response index with sensitive response characteristics to different types of toxic pollutants. In order to solve this problem, in this study, the influences of six typical toxic pollutants belonging to different types, including the heavy metal cadmium, the herbicide atrazine, organic chemical raw materials 1,4-benzoquinone and 1,4-dichlorobenzene, and the disinfection by-products chloroacetic acid and trichloroacetonitrile on the fluorescence rise kinetics (OJIP) curve of microalga Chlorella pyrenoidosa were investigated. According to the same characteristics of changes in the OJIP curves of different toxic pollutant treatments, a new response index for detecting the comprehensive toxicity of water (RI ct ) was constructed. By comparing with the maximum photochemical quantum yield of photosystem II (PSII) (F v /F m ), the potential quantum yield of PSII (F v /F o ), the performance index of PSII (PI ABS ) and the quantum yield of electron transport of PSII (φ Eo ), the feasibility and sensitivity of quantitative response of RI ct to the toxicity of each pollutant and polluted water containing multiple pollutants were verified. The results indicated that all the six toxic pollutants had the same influence on parameters F v(m-j) /F o and F v(m–o) /F o contained in the OJIP curve, and RI ct constructed based on F v(m-j) /F o and F v(m–o) /F o had a good logistic curve concentration–response relationship with each pollutant. By comparison, the response sensitivity of RI ct to all pollutants was significantly superior to F v /F m , F v /F o and φ Eo , and also had better stability in the toxicity response to each pollutant compared to PI ABS , indicating that RI ct was a suitable response index for rapidly and sensitively detecting water comprehensive toxicity based on microalgal fluorescence kinetics. This study provides an important basis for the development of rapid and sensitive detection methods for water toxicity.
研究领域:
环境科学
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生物学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Ying Shi,Yi-Wen Wu,Qian-Xue Shen,Jun Cao
Aristolochic acids are one of the major compounds in aristolochia plants, which are nephrotoxic and carcinogenic. A method was established for the detection and identification of aristolochic acids and their DNA adducts in four different herbs using ultra-high performance liquid chromatography-ion mobility quadrupole time-of flight mass spectrometry. Solid phase extraction conditions were optimized to improve the sensitivity of the experiment by using 40 mg of C 18 as adsorbent and 100 μL ethanol as elution solvent. At a collision energy of 10-40 eV, these compounds and cleavage patterns were precisely identified and analyzed by secondary fragmentation and collision cross section values. The obtained mass spectrometry data were then analyzed by targeted metabolomics, including principal component analysis, partial least squares-discriminant analysis and hierarchical clustering analysis, and importing the samples in the established model, the confidence values can reach 0.61 and 0.76. All in all, this method can provide a useful tool for the detection of aristolochic acids and deoxyribonucleic acid adducts. In conclusion, this method was successfully used for the detection and identification of aristolochic acids and their DNA adducts.
研究领域:
药理学
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毒理学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
Food Science & Nutrition
·
2022
·Vol. 10
·期 6
·1841-1853
·
IF 3.553
Jun Yang,Shulin Hu,Anqi Liao,Yetian Weng,Shuli Liang,Ying Lin
Current chemical analysis approaches for contaminants have failed to reveal their biotoxicity. Moreover, conventional bioassays are time consuming and exhibit poor repeatability. In this study, we performed the acute toxicity detection of various contaminants (chromium (Cr), cadmium (Cd), lead (Pb), arsenic (As), mercury (Hg), tin (Sn), nickel (Ni), and bisphenol A (BPA)) with four bioluminescent bacteria ( Vibrio qinghaiensis Q67, V. fischeri , Photobacterium phosphoreum T3, and P. phosphoreum 502) using a rapid, flexible, and low-cost bioassay. We found that the temperature affected the bacterial luminescence, and freeze-dried cells exhibited sensitive toxic responses to contaminants. Indeed, the optimized protectants containing 12% (w/v) trehalose, 4% sucrose, and 2% sorbitol displayed better luminescence and toxic sensitivity. Furthermore, freeze-dried powders of these strains were prepared and subjected to acute toxicity detection. The results showed that all contaminants exhibited acute toxicity toward Q67, but the other strains did not show obvious response to nickel and tin. The relative half-maximal effective concentration (EC 50 ) values of BPA, Cr, Cd, Pb, As, Hg, Ni, and Sn to Q67 were 0.674, 1.313, 11.137, 5.921, 4.674, 0.911, 5.941, and 54.077 mg/L, respectively. In addition, the EC 50 values of contaminants toward different strains were suggested to be statistically significant. Freeze-dried Q67 exhibited toxic responses to more contaminants than the other bioluminescent strains; therefore, Q67 was selected to be more suitable than the other strains for single and mixture toxicity detection tests. Compared with other strains, Q67 was more appropriate for the rapid screening of the mixture toxicity of contaminants in samples as a nonspecific screening sensor before the use of standard analysis approaches.
研究领域:
环境科学
·
毒理学
·
生物技术
·
化学分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2023
·
IF 5.8
Xinyue Xing,Wanqing Zhong,Ping Tang,Qiao Tao,Xiaoxu Lu,Liyun Zhong
The novel histone deacetylase drug chidamide (CHI) has been proven to regulate gene expression associated with oncogenesis via epigenetic mechanisms . However, huge side effects such as non-targeting, poor intracellular accumulation and low nuclear entry efficiency severely restrict its therapeutic efficacy. Dual-targeted nanodrug delivery systems have been proposed as the solution. Herein, we developed a CHI-loaded drug delivery nanosystem based on Prussian blue (PB) nanocarrier , which combines surface-enhanced Raman scattering (SERS) tracking function with cancer cell/nuclear-targeted chemotherapy capability. With the property of background-free SERS mapping, PB nanocarriers can serve as tracking agents to localize intracellular CHI. The incorporation of targeted molecules specifically enhances the cancer cell/nuclear internalization and chemotherapeutic effects of CHI-loaded PB nanocarriers. In vitro cytotoxicity assay clearly shows that the constructed CHI-loaded PB nanocarriers have significant inhibitory on Jurkat cell proliferation . Furthermore, SERS spectral analysis of Jurkat cells incubated with the CHI-loaded PB nanocarriers reveals obvious features of cellular apoptosis: DNA skeleton fragmentation, chromatin depolymerization , histone acetylation, and nucleosome conformation change. Importantly, this CHI-loaded PB nanocarrier will provide a new insight for lymphoblastic leukemia targeted chemotherapy.
研究领域:
药学
·
纳米技术
·
癌症研究
·
分子生物学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）