CHEMOSPHERE
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2022
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IF 8.943
Jie Cheng,Kaidian Zhang,Jiashun Li,Yuyong Hou
Increased hazardous substances application causes more environmental pollution and risks for human health. Microalgae are the important biological groups in marine ecosystem, and considered to be sensitive to environmental pollutants. Therefore, toxicity test on marine microalgae could provide the most efficient method for aquatic toxicity assessment, and could also be used as the early warning signals in aquatic ecosystem . In view of this, our study aimed at investigating the toxicity potential of two typical organic compounds, and screening out novel photosynthetic indicators for the risk assessment of environmental pollutants. In this study, benzyl alcohol and 2-phenylethanol were chosen as the target organic compounds, and preliminary toxicity mechanism of these organic compounds on marine cyanobacterium Synechococcus sp. PCC7002 was investigated with chlorophyll fluorescence technology. Results showed that PCC7002 could be affected by benzyl alcohol or 2-phenylethanol stress, and the toxicity effect was concentration-dependent. And external benzyl alcohol and 2-phenylethanol stress damaged the oxygen evolving complex, and suppressed electron transport at the donor and receptor sides of photosystem II (PSII), influencing the absorption, transfer, and application of light energy. Furthermore, potential biomarkers were screened by half maximal inhibitory concentration (IC 50 ) on the basis of pearson correlation coefficient analysis, and fluorescence intensity difference between the I-step and P-step of OJIP curve (δF IP ) seems to be the most sensitive indicator for external stress. This study would be of significant interest to the biomarker community, and pave the way for the practical resource for marine pollution monitoring and assessment.
研究领域:
生态毒理学
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环境科学
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DOI
PubMed
高影响因子
AQUATIC TOXICOLOGY
·
2025
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IF 4.3
Wanglong Zhang,Yanjiao Zong,Ruize Sun,Zhenhong Xue,Wenhui Wan,Anran Ren,Yongchao Ma,Wenjing Tian,Renjun Wang
The global water pollution now calls for precise risk assessment of chemicals, e.g., dioxins and the dioxin-like compounds (DLCs). The freshwater and marine medaka have been widely implemented in the toxicity testing, and perhaps give mechanistic information for comparative biology. The question that ‘will they report equal results due to their close phylogenetic relation’ has been raised, therefore, we explored their physiological and molecular responses to dioxin. As the mediator of the dioxin toxicity, the aryl hydrocarbon receptor (AHR) of marine medaka ( Oryzias melastigma ) has not been functionally characterized and might be species-specific. In terms of sensitivity to dioxin—2,3,7,8-tetrachlorodibenzo- p -dioxin (TCDD), the EC 50 values of omeAHR1a (0.16±0.12 nM), omeAHR1b (2.96±2.96 nM), omeAHR2a (0.44±0.30 nM), and omeAHR2b (9.00±6.88 nM) exhibit marked variations. The omeAHR2a and omeAHR1a display heightened sensitivity compared to the freshwater Japanese medaka ( Oryzias latipes ) counterparts olaAHR2a and olaAHR1a, respectively. The results indicate the in vitro sensitivity of AHR among species can vary by one or two orders of magnitude. Further mechanistic investigations using additional ligands and computational modeling reveal that: 1) most of omeAHR2a, olaAHR2a, dreAHR2, and hsaAHR interact with ligands in the affinity order of TCDD > PCB126 > BNF > indole, mirroring their AHR transactivation potency, but the docking poses and dynamics can vary; 2) one AHR subform's high sensitivity to dioxin—TCDD may extend to DLCs but not to other types of ligands. Beyond the in vitro study, the preliminary in vivo LC 50 data indicate that marine medaka (LC 50 : 1.64 ng/L (95% CI: 1.05-2.55 ng/L)) has similar sensitivity, and possibly slightly greater (not statistically determined yet), to TCDD in comparison with Japanese medaka (LC 50 : 3.42 ng/L (95% CI: 1.37-6.48 ng/L)).These insights underscore the difference of AHR biology among species even the close relative species, and point out the necessity for meticulous consideration when evaluating the toxicity of compounds and when extending predictive toxicity assessments to more species.
研究领域:
环境科学
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分子生物学
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毒理学
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DOI
PubMed
高影响因子
OPTICAL MATERIALS
·
2023
·
IF 3.9
Fan Yang,Sheng-Tong Wu,Xiao-Qiong Lei,Xue-Lian Han,Ping Chen,Shou-Nian Ding
Traditional contact lenses patterns are mostly colored with organic pigments, but they suffered a pigment leakage risk after long-term wearing, which causes corneal inflammation and ulceration. To solve this issue, the structural color is applied to contact lenses because it has color and pigment free. Herein, we reported the application of SiO 2 photonic crystal microspheres in contact lenses. We successfully prepared SiO 2 photonic crystal ink with structural color and fabricated contact lenses by ink transfer printing, and these contact lenses also contain patterns and pupil areas that are the same as the traditional pigment one. In addition, in vitro cytotoxicity test, physicochemical property test, and optical property test were carried out to verify the performance of the prepared structural color lenses. All results suggested it has great potential to replace traditional organic pigment contact lenses.
研究领域:
材料科学
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生物医学工程
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眼科
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DOI
高影响因子
WATER SCIENCE AND TECHNOLOGY
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2024
·Vol. 89
·期 3
·811-822
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IF 2.5
Ma Yang,Ma Yongwen,Wan Jinquan,Wang Yan,Ye Gang,Zhang Zhifei,Lin Yining
View large Download slide View large Download slide Close modal Advanced oxidation technologies based on hydroxyl radical (•OH) and sulfate radical (SO 4 −• ) are two common types of advanced oxidation technologies, but there are not many reports on the application of advanced oxidation methods in actual wastewater pretreatment. This article compares the pre-treatment performance of Fe 2+ /H 2 O 2 and Fe 2+ /Persulfate systems in actual pharmaceutical wastewater, and combines EEM, GC-MS, and toxicity testing results to explore the differences in TOC, COD, and NH 3 -N removal rates, optimal catalyst dosage, applicable pH range, toxicity of effluent after reaction, and pollutant structure between the two systems. The results indicate that the Fe 2+ /H 2 O 2 system has a higher pollutant removal rate (TOC: 71.9%, COD: 66.9%, NH 3 -N: 34.1%), but also requires a higher catalyst (Fe 2+ ) concentration (6.0 g/L), and its effluent exhibits characteristic peaks of aromatic proteins. The Fe 2+ /Persulfate system has a wider pH range (pH ≈ 3-7) and is more advantageous in treating wastewater containing more cyclic organic compounds, but the effluent contains some sulfur-containing compounds. In addition, toxicity tests have shown that the toxicity reduction effect of the Fe 2+ /Persulfate system is stronger than that of the Fe 2+ /H 2 O 2 system. Two types of advanced oxidation systems were compared using actual pharmaceutical wastewater as the research object. Investigated the pretreatment effect of advanced oxidation systems and explored the possibility of applying advanced oxidation to the pretreatment process of wastewater. A deeper comparison was made between the two advanced oxidation methods by comparing the EEM and GC–MS results.
研究领域:
环境科学
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废水处理工程
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产品关联
DOI
PubMed
高影响因子
Journal of Zhejiang University-SCIENCE B
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2018
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IF 1.815
Yi-ying Zhao,Qiang Chu,Xu-er Shi,Xiao-dong Zheng,Xiao-ting Shen,Yan-zhen Zhang
To address the controversial issue of the toxicity of dental alloys and silver nanoparticles in medical applications, an in vivo-like LO2 3-D model was constructed within polyvinylidene fluoride hollow fiber materials to mimic the microenvironment of liver tissue. The use of microscopy methods and the measurement of liver-specific functions optimized the model for best cell performances and also proved the superiority of the 3-D LO2 model when compared with the traditional monolayer model. Toxicity tests were conducted using the newly constructed model, finding that four dental castings coated with silver nanoparticles were toxic to human hepatocytes after cell viability assays. In general, the toxicity of both the castings and the coated silver nanoparticles aggravated as time increased, yet the nanoparticles attenuated the general toxicity by preventing metal ion release, especially at high concentrations.
研究领域:
材料科学
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毒理学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2018
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IF 2.829
Yue Zhang,Tingting Ren,Junhui He,Hua Tian,Binbin Jin
Toxicity test sensor for heavy metal ions is developed in this work. It founds that toxic heavy metal ions leads both bacterial enzymatic activity and viability. Moreover, the hydrogel encapsulation of bacteria was performed to achieve two purposes: (1) to enhance the viability/activity of the biological sensing agent and thus prolong the shelf life of biosensors based on bacteria; and (2) to further improve the portability of the bioassay system and simplify the bioassay operation. The developed bioassay demonstrates potentials in comprehensive toxicity test of real water samples containing multiplex heavy metal ions. This work paves the way for on-site biotoxicity test based on microorganisms and provides clues for the development of long-term validity biosensors.
研究领域:
环境科学
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生物技术
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微生物学
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DOI
高影响因子
Scientific Reports
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2017
·Vol. 7
·期 1
·1-10
·
IF 4.259
Chu Qiang,Zhao Yiying,Shi Xuer,Han Wen,Zhang Yanzhen,Zheng Xiaodong,Zhu Jing
To address the need for a high throughput toxicity test in the modern food industry, an in vivo -like 3-D cell model was constructed in this study to provide an alternative to controversial long-term animal models and to improve the sensitivity and accuracy of the traditional monolayer model. The model formed cell cylindroids within polyvinylidene fluoride (PVDF) hollow fibers and therefore mimicked the microenvironment of liver tissue. Microscopy methods were used, and liver-specific functions were measured to demonstrate the superiority of the model compared to the monolayer model, as well as to optimize the model for best cell performances. Later, toxicity tests of sodium nitrite and acrylamide were conducted in both the 3-D model and the monolayer model to study the sensitivity of the 3-D model in toxicity responses. As expected, HepG2 cells within the 3-D model responded at lower concentrations and shorter exposure times compared to cells within the monolayer model. Furthermore, western blot analysis of apoptosis pathways also supported the argument.
研究领域:
毒理学
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细胞生物学
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食品科学
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产品关联
DOI
PubMed
高影响因子
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
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2022
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IF 7.926
Yingzi Lin,Jing Chen,Mingliang Zhou,Gen Liu,Siwen Li,Chunyan Shi,Mengshi Wang,Shenglin Qi
In this paper, the efficiency and mechanism of using nitrilotriacetic acid(NTA) as a complexing agent to enhance the zero-valent iron(Fe 0 )/peroxymonosulfate(PMS) system to remove sulfamethazine(SMZ) from water are discussed. Research results show that the addition of NTA increases the soluble iron by 3.05 times, and the decomposition rate of PMS increases from 27% to 62%. Keeping other conditions unchanged and increasing the concentration of PMS to 0.2 mM, the degradation rate reaches the peak, continued increase of PMS concentration will decrease the degradation rate. The degradation rate of SMZ is positively correlated with Fe 0 concentration. The effect of different inorganic ions on the degradation of SMZ was investigated. The high concentration of Cl − promoted the degradation effect, and the reaction rate constant of the system is increased by 1.67 times when the Cl − dosing is 10 mM. The PMS/Fe 0 /NTA system degrades SMZ up to 79.36% in actual water bodies. The degradation of SMZ is mainly by Smiles-type rearrangement, sulfonamide bond breaking, etc. The toxicity test proves that the inhibition rate of luminescent bacteria before and after degradation decreases from 61.86% to 40.47%, with an overall decreasing trend of toxicity.
研究领域:
环境科学
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水处理
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化学工程
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毒理学
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产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2020
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IF 6.551
Xiang Li,Jiao Dang,Yan Li,Lizhen Wang,Ning Li,Kechun Liu,Meng Jin
Background Larval zebrafish ( Danio rerio ) is not only an ideal vertebrate applied in Fish Embryos Toxicity (FET) test but also a well-accepted model in behavioral neurotoxicity research. By applying the commercial standard behavioral tracking system (Zebrabox), the locomotion profiles (neurobehavioral-phenomics) of larval zebrafish can be comprehensively monitored and systematically analyzed to probe ecotoxicological neurotoxicity of nano-pollutants at environmental relevant concentration level. Results Herein, the potential toxicity of at environment relevant concentration level on embryonic zebrafish was evaluated by FET and neurobehavioral-phenomics (NBP). The embryos were exposed to the environmental relevant concentration (0.05, 0.1,1, 5, 10, 100 μg/L). The FET criteria were utilized to evaluate the ecotoxicological effect induced by silica NPs. Subsequently, behavioral neurotoxicity of silica NPs was further quantified via locomotion response (LMR). Specifically, the alteration of Light/Dark challenge (LDC) evoked by light/dark stimulation was detected and analyzed by commercially standard behavioral protocols using zebrabox. We revealed that the exposures of silica NPs at environmental relevant concentration (0.05, 0.1, 1, 5, 10,100 μg/L) significantly disturbed locomotion profiles of larval zebrafish. Additionally, it was obviously noted that low, environmentally relevant silica concentrations might result in altering the total behavioral profiles in developing zebrafish. Conclusions In sum, neurobehavior phenomics profiling based on LMR and LDC is a potent methodology for the evaluation of sub-lethal or sub-teratogenic toxicity. Compared with the FET tests characterized by the detection of embryonic teratogenicity, the neurobehavior phenomics based method can be more sensitive to determine sub-teratogenic toxicity of silica NPs at environmental concentrations. With the combination of multivariate data analysis, this approach would offer effective technical reference for environmental nano-toxicology research.
研究领域:
环境科学
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毒理学
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神经科学
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水生生物学
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产品关联
DOI
PubMed
高影响因子
INORGANICA CHIMICA ACTA
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2018
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IF 2.264
Yang Zhang,Jian Wang
A water-stable 2D Zn-based MOF with chemical formulae [(Me 2 NH 2 ) 4 Zn 2 (FDC) 4 ] n ( 1 ) (FDC = 2,5-furandicarboxylate) was synthesized and structurally characterized. TGA and PXRD results show high thermal and water stability for 1 . 1 exhibits a 2D (4,4) net with 1D channel of 7.2 × 7.2 Å 2 along the c axis. Desolvated 1 adsorbs around 11.4 wt% 5-Fluorouracil and the cumulative release value of 5-Fluorouracil at 48 h was about 96.5% and 99% in PBS (pH = 7.4 and pH = 6.0) at 37 °C, respectively. The in vivo Zebrafish toxicity tests and in vitro MTT assays of 1 at different concentrations results reveal that 1 is nontoxic.
研究领域:
材料科学
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药学
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生物医学工程
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产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2022
·
IF 9.136
Yuhan Long,Songrong Li,Peizhen Yang,Xi Chen,Wenhao Liu,Xuesong Zhan,Cheng Xue,Dongfang Liu,Wenli Huang
MOF-derived metal-carbon composite catalyst was an alternative high-efficient catalyst for activating peroxymonosulfate (PMS). In this study, a novel cobalt-carbon-nitrogen doped composite catalyst with honeycomb porous structure (Co-HPNC) was obtained through a simple strategy consisting of three steps: self-growth of ZIF-67 on the SiO 2 core, pyrolysis, and NaOH etching away SiO 2 core. The Co-HPNC exhibited an outstanding catalytic performance (98.1% in 10 min) to activate PMS for acid orange 7 (AO7) degradation. The observed reaction kinetic constant (0.4319 min −1 ) was 2.64 times superior to that of the unmodified Co/NC catalysis system (0.1636 min −1 ). In the meantime, the catalyst demonstrated a high PMS activation capacity over a wide pH range of 3–11 and strong adaptability to the high concentration of inorganic anions (Cl - , SO 4 2- , and CO 3 2– ). After being recycled three times, the removal efficiency could still reach 92.7% in 25 min. The quenching tests and EPR spectrum illustrated the existence of radical and non-radical pathways, in which singlet oxygen was dominant during the degradation process. It was hypothesized that Co(0)/Co(Ⅱ)/Co(Ⅲ), pyridinic-N and pyrrolic-N contributed to the generation of hydroxyl and sulfate radicals, while C=O functional group and graphitic-N were catalytic sites for singlet oxygen. Finally, the radical electrophilic attacking sites of AO7 were calculated by the density functional theory (DFT), and the degradation routes of AO7 were speculated by GC–MS. The toxicity test illustrated that the Co-HPNC/PMS system was an environmental-friendly technology for AO7 degradation. The prepared Co-HPNC with a unique honeycomb porous structure presents a novel synthesis route for MOF-derived catalyst applied in the PMS activation system for wastewater treatment.
研究领域:
环境科学
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催化
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材料科学
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水处理
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化学工程
✓ 已验证
产品关联
DOI
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
·
2019
·
IF 4.708
Shuang Li,Jiaxin Liu,Ge Li,Xueyan Zhang,Fei Xu,Zhijiang Fu,Lesheng Teng,Youxin Li,Fengying Sun
Parkinson’s disease (PD) is associated with symptoms such as tremor and bradykinesia which, together with a rigorous dosing regimen, can place an untenable burden on patients. These issues underscore the need for triggerable, modulated drug delivery systems . Currently, pramipexole (PRX) is the most widely used non-ergot dopamine agonist for the treatment of PD. In this study, near-infrared light-responsive PRX and hollow gold nanospheres (HGNS)-loaded biodegradable poly (D, L-lactide-co-glycolide) (PLGA) microspheres (PRX/HGNS MS) were fabricated using solid-in-oil-in-water (S/O/W) and water-in-oil-in-water (W/O/W) emulsion-solvent evaporation techniques to achieve modulated drug release. The PRX/HGNS MS were uniform, with an average diameter of approximately 24 µm, favorable PRX and HGNS encapsulation efficiencies (51.71 ± 0.54% and 65.15 ± 2.30%, respectively) and rapid, controllable drug release both in vitro and in vivo . Cytotoxicity tests revealed no significant differences between HGNS and PRX/HGNS MS when compared with a negative control. Pharmacodynamics and immunohistochemistry studies revealed a more rapid recovery of striatum in the group treated with PRX/HGNS MS produced using the S/O/W method. The results clearly demonstrate that light-responsive PRX/HGNS MS produced using the S/O/W method have the potential to address PD patients’ mobility problems in a smart, controllable and remotely triggerable manner.
研究领域:
神经学
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药学
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纳米技术
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）