Wenhui Zeng,Bingrong Liu,Wenjing Wu,Shijun Zhang,Yong Chen,Zhiqiang Li
Simple SummaryCoptotermes formosanusShiraki is a wood feeding lower termite and is widely distributed in many areas. The dynamic adjustment of theC. formosanusdigestive system to unfavorable survival environments was investigated via non-lethal toxic feeding. The toxic stress did not change the dominant role of microbial lignocellulases in cellulose degradation ofC. formosanus. The core symbiotic community was stable in abundance during the tolerance to the toxic treatment. However, a large number of low abundance taxa were significantly enriched by the low toxic feeding. These rare bacterial lineages likely contribute to toxic stress tolerance of termite. Above all, these findings add important new knowledge to our understanding of environmental adaptation of the lignocellulose hydrolysis system in termites.AbstractDisturbing the lignocellulose digestive system of termites is considered to be a promising approach for termite control. The research on the tolerance mechanism of the termite lignocellulose digestive system to harmful environment conditions is limited. In this study, we keepCoptotermes formosanusSkiraki under a non-lethal toxic condition by feeding the termites with filter paper containing the kojic acid (a low toxic insecticide). The effects of low toxic stress on the activities and gene expressions of host/symbiotic originated lignocellulases, and on the symbiotic microbial community structure ofC. formosanuswere explored. Our result showed that the low toxic stress would lead to the synchronous decrease of cellulase and hemicellulase activities, and supplementary increase of corresponding gene expressions. The symbiotic community maintained its role as the main force in the lignocellulolytic system ofC. formosanus. Meanwhile, a large number of rare taxa were significantly enriched by kojic acid treatment. These numerically inconspicuous bacterial populations might be responsible for the functions similar to phenoloxidase or insecticide detoxification and enableC. formosanusto tolerate the harmful environment. Overall, our data suggested that the digestive adaptation ofC. formosanusto physiotoxic feeding is closely related to the triple collaboration of termites–flagellates–bacteria.Keywords:lignocellulolytic system;non-lethal stress;symbiont;termite;toxic tolerance
Sulfadiazine (SDZ) as a common sulfonamide antibiotic is frequently detected in wastewater, but there is little information on the high-value product recovery and toxicity tolerance evaluation of mixotrophic microalgae under SDZ stress. In this study, effects of SDZ on growth, photosynthesis, cellular damage, antioxidant capacity and intracellular biochemical components of Chlorella pyrenoidosa were investigated. Results showed that the growth of C. pyrenoidosa was inhibited by about 20% under high SDZ stress, but there was little impact on photosynthesis. Cellular damage and antioxidant capacity were evaluated using malondialdehyde (MDA) content and superoxide dismutase (SOD) activity to further explain the toxicity tolerance of mixotrophic microalgae. The SDZ stress not only increased lipid and carbohydrate content, respectively attaining to the maximum of 390.0 and 65.4 mg/L, but also improved the biodiesel quality of C. pyrenoidosa . The findings show the potential of mixotrophic microalgae for biodiesel production and wastewater treatment.
Abnormal high level of cell free DNA (cfDNA) triggers chronic inflammation to exacerbate psoriasis symptoms. Scavenging cfDNA by topical cationic polymeric nanoparticles has been certified as an effective therapeutic strategy for treating psoriasis. However, cationic cfDNA scavengers have a great potential risk to organs after entering systemic circulation through skin barrier. For better transformation to clinical application, herein a series of poly(2-(dimethylamino)ethyl methacrylate) (PDMA) grafted hairy silica particles (cSPs) with tunable PDMA length and particle size are applied to scavenge cfDNA in dermis. We reveal that the structure of cSPs correlates with the permeation ability across stratum corneum, retention time in dermis, binding affinity to cfDNA, and toxicity tolerance, which in turn affect the therapeutic effect. Especially, the cSPs of 700 nm show more accumulation and longer retention in psoriatic lesions, leading to excellent treatment results. They also outperform the cSPs of 200 nm at a lower administration frequency. Thus, we address the issues of size, cationic content of cSPs to open a potential new avenue to topically treatment of psoriasis by targeting cfDNA in dermis.
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