ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
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2022
·
IF 5.938
Yuan-Yuan Dai,Chun Qin,Gan-Rong Huang,Yan-Chun Qin,Yong-Yi Huang,Yan-Qiang Huang,Li-juan Zhao
Metronidazole (Met) is the first choice for treating Helicobacter pylori (Hp). However, Hp is easy to resistant, making Met unable to be widely used. How to overcome Hp’s Met resistance is still an issue. In this study, Met was used as the primary raw material with linolenic acid to prepare a novel compound-linolenic acid-metronidazole (Lla-Met). The MIC, minimum bactericidal concentration (MBC), colonization amount of Hp in gastric mucosa, etc., were evaluated, respectively. Lla-Met was successfully prepared by the detection of nuclear magnetic resonance, etc., and its MIC and MBC to Hp were 2~4 μg/mL, 8~16 μg/mL. Moreover, in vivo experiments, Lla-Met significantly reduced the colonization of drug-resistant Hp in gastric mucosa. In the toxicity test, Lla-Met inhibited rate to GES-1 and BGC823 cells were 15% at 128 μg/mL; the mice were administered 10 times treatment Lla-Met treatment (240 mg/kg), have no difference significant injuries were found in their stomach, liver, spleen, kidney, and weight. In addition, Hp G27 continued for 18 days in vitro with sub-Lla-Met concentration, G27 did not show drug resistance to Lla-Met; Lla-Met did not exert an effect on non-Hp species with 128 μg/mL; Compared with a neutral environment, when the acid concentration is 3.0, Lla-Met is not decomposed and has better stability. Conclusion: Lla-Met, a newly prepared compound, has relatively well antibacterial of Met-resistant and sensitive Hp, with a capability of overcoming the metronidazole resistance of Hp.
研究领域:
药理学
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微生物学
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医学研究
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DOI
PubMed
高影响因子
Frontiers in Pharmacology
·
2022
·
IF 5.988
Shen Yunfu,Guo Kaiqiang,Ma Aijun,Huang Zhe,Du Jingjing,Chen Junhe,Lin Qianyu,Wei Chengming,Wang Zi,Zhang Fuming,Zhang Juan,Lin Wanjun,Feng Na,Ma Wenzhe
There are technical obstacles in the safety evaluation of traditional Chinese medicine (TCM) injections due to their complex chemical nature and the lack of rapid and accurate in vitro methods. Here, we established a dual in vitro mitochondrial toxicity approach combing the conventional “glucose/galactose” assay in HepG2 cells with the cytotoxic assay in mitochondrial respiration deficient cells. Using this dual in vitro approach, for the first time, we systematically assessed the mitochondrial toxicity of TCM injections. Four of the 35 TCM injections, including Xiyanping, Dengzhanhuasu, Shuanghuanglian, and Yinzhihuang, significantly reduced cellular ATP production in galactose medium in the first assay, and presented less cytotoxic in the respiration deficient cells in the second assay, indicating that they have mitochondrial toxicity. Furthermore, we identified scutellarin, rutin, phillyrin, and baicalin could be the potential mitochondrial toxic ingredients in the 4 TCM injections by combining molecular docking analysis with experimental validation. Collectively, the dual in vitro approach is worth applying to the safety evaluation of more TCM products, and mitochondrial toxic TCM injections and ingredients found in this study deserve more attention.
研究领域:
药理学
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毒理学
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中药
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产品关联
DOI
PubMed
高影响因子
TOXICON
·
2022
·
IF 3.035
Xue-Lin Chen,Bing Li,Xia Zhao,Li-hong Li,Han-Lei Wang,Kun Zhang,Mei Han,Yu-Mei Zhang
Kochiae Fructus (KF) was listed as ‘top grade’ medicinal material by the ‘Shennong's Herbal Classic of Materia Medica’ and has been used in traditional Chinese medicine to delay aging and treat inflammation, such as rubella, eczema, cutaneous pruritus, etc. Our research focused on the antioxidant capability of water decoction and fractions from KF based on 2,2-iphenyl-1-picrylhydrazyl (DPPH) free radical and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation radical scavenging assay, the ferric reducing antioxidant power assay, and inhibitory effects on DNA and protein oxidative damage. The results of total phenolics and flavonoids contents showed that ethyl acetate fraction (EAF) possessed the highest phenolics and flavonoids with values of 112.90 ± 9.58 mg gallic acid equivalents/g and 329.60 ± 20.93 mg rutin equivalents/g, respectively. At the same time, the results of antioxidant capacities showed that EAF possessed best antioxidant abilities. In addition, in this work, we evaluated the oral safety of the water decoction of KF (KFWD) via the 14-day acute and 28-day subacute toxicity tests. The results of in vivo toxicity assessment showed that KFWD did not cause significant changes in the general clinical symptoms, hematology and biochemical parameters, organ weights, or histopathological appearances in mice or rats. In summary, the reason why KF has the traditional effect on delaying aging may be related to the fact that its rich in flavonoids and phenolics. Simultaneously, no toxicity was detected after acute or subacute treatment of KFWD, providing valuable evidence for the traditional safe use of KF.
研究领域:
药理学
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毒理学
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中医药
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抗氧化研究
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产品关联
DOI
PubMed
高影响因子
Journal of the Mechanical Behavior of Biomedical Materials
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2023
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IF 3.9
Rongfang Zou,Lunan Bi,Yang Huang,Yadi Wang,Yan Wang,Lin Li,Jiayin Liu,Lu Feng,Xiaoxia Jiang,Bin Deng
For decades, titanium has been the preferred material for dental implant fabrication. However, metallic ions and particles can cause hypersensitivity and aseptic loosening. The growing demand for metal-free dental restorations has also promoted the development of ceramic-based dental implants, such as silicon nitride . In this study, silicon nitride (Si 3 N 4 ) dental implants were fabricated for biological engineering by photosensitive resin based digital light processing (DLP) technology, comparable to conventionally produced Si 3 N 4 ceramics. The flexural strength was (770 ± 35) MPa by the three-point bending method, and the fracture toughness was (13.3 ± 1.1) MPa · m 1/2 by the unilateral pre-cracked beam method. The elastic modulus measured by the bending method was (236 ± 10) GPa. To confirm whether the prepared Si 3 N 4 ceramics possessed good biocompatibility , in vitro biological experiments were performed with the fibroblast cell line L-929, and preferable cell proliferation and apoptosis were observed at the initial stages. Hemolysis test, oral mucous membrane irritation test, and acute systemic toxicity test (oral route) further confirmed that the Si 3 N 4 ceramics did not exhibit hemolysis reaction, oral mucosal stimulation, or systemic toxicity. The findings indicate that Si 3 N 4 dental implant restorations with personalized structures prepared by DLP technology have good mechanical properties and biocompatibility, which has great application potential in the future.
研究领域:
牙科材料
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生物医学工程
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材料科学
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生物相容性测试
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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2022
·Vol. 30
·期 8
·21104-21114
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IF 5.19
Xu Xia,Guo Jun,Gao Yu,Xue Yingang,Shi Xinlan,Zhang Ling,Zhang Qiuya,Peng Mingguo
Take-away containers are the common food contact materials (FCMs) that are widely used in daily life. However, little is known regarding the effects of different food simulants on the pollution characteristics of microplastics derived from food containers, as well as the toxic effects of the chemical substances that are leached from them. Extracts were obtained by adding organic solvents into plastic containers (polypropylene, PP; polystyrene, PS) to simulate aqueous, alcoholic, and fatty environments. The extracted substances and their toxic effects were then assessed by counting and characterizing the resulting microplastics and performing bio-acute toxicity assays. The results demonstrated that the highest abundance of microplastics occurred in PS containers in fatty environments, which was likely due to the rough surface of the PS. In contrast, organic solvents seemed more conducive to the migration of substances. Furthermore, the PP and PS extracts in an alcohol and fatty environment have significant impacts on zebrafish embryo development, including arrhythmia, pericardial cysts, and spinal curvature. Graphical abstract
研究领域:
环境科学
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毒理学
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材料科学
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水生生物学
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DOI
PubMed
高影响因子
Mucosal Immunology
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2024
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IF 7.9
Xixi Huang,Tingxuan Yin,Min Yu,Guohua Zhu,Xianyang Hu,Hailin Yu,Weijie Zhao,Jiajia Chen,Jiangyuan Du,Qingyu Wu,Wei Zhang,Lu Liu,Meirong Du
Decidual CD8 + T (dCD8 + T) cells are pivotal in the maintenance of the delicate balance between immune tolerance towards the fetus and immune resistance against pathogens. The endometrium and decidua represent uterine environments before and during pregnancy, respectively, and the composition and phenotypic alterations of uterine CD8 + T cells in these tissues remain unclear. Using flow cytometry and analysis of transcriptome profiles, we demonstrated that human dCD8 + T and endometrial CD8 + T (eCD8 + T) cells exhibited similar T cell differentiation statuses and phenotypes of tissue infiltrating or residency, compared to peripheral CD8 + T (pCD8 + T) cells. However, dCD8 + T cells showed decreased expression of coinhibitory (PD-1), chemotaxis (CXCR3), and tissue-resident (CD69 and CD103) markers and increased granzyme B and granulysin expression compared to eCD8 + T cells. In vitro, cytotoxicity assays demonstrated greater effector functions in dCD8 + T cells than in eCD8 + T cells. Further in vitro and in vivo chemotaxis assays confirmed the recruitment of non-resident effector memory T cell subsets to the pregnant decidua, contributing to the dCD8 + T cell-mediated anti-infection mechanism at the maternal-fetal interface. This work demonstrated dCD8 + T cells replenished from the circulation retained their cytotoxic capacity, which may serve as an enhanced defense mechanism against infection during pregnancy.
研究领域:
免疫学
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生殖生物学
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妊娠免疫学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Shuaishuai Xing,Ying Chen,Baichen Xiong,Weixuan Lu,Qi Li,Yuanyuan Wang,Mengxia Jiao,Feng Feng,Yao Chen,Wenyuan Liu,Haopeng Sun
In recent years, butyrylcholinesterase (BChE) has gradually gained worldwide interests as a novel target for treating Alzheimer's disease (AD). Here, two pharmacophore models were generated using Schrödinger suite and used to virtually screen ChemDiv database, from which three hits were obtained. Among them, 2513–4169 displayed the highest inhibitory activity and selectivity against BChE ( ee AChE IC 50 > 10 μM, eq BChE IC 50 = 3.73 ± 1.90 μM). Molecular dynamic (MD) simulation validated the binding pattern of 2513–4169 in BChE, and it could form a various of receptor-ligand interactions with adjacent residues. In vitro cytotoxicity assay proved the safety of 2513–4169 on diverse neural cell lines. Moreover, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay performed on SH-SY5Y cells proved the neuroprotective effect of 2513–4169 against toxic Aβ 1 – 42 . In vivo behavioral study further confirmed the great efficacy of 2513–4169 on reversing Aβ 1 – 42 -induced cognitive impairment of mice and clearing the toxic Aβ 1 – 42 in brains. Moreover, 2513–4169 was proved to be able to cross blood-brain barrier (BBB) through a parallel artificial membrane permeation assay of BBB (PAMPA-BBB). Taken together, 2513–4169 is a promising lead compound for future optimization to discover anti-AD treating agents.
研究领域:
神经科学
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药理学
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药物发现
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Chen Shi,Jian Zhang,Pan Wu,Min Xia,Qing Jiang,Xiong Yang,Yafei Shi,Gary Owens,Kewu Pi
Landfill leachate (LFL) typically contains a wide range of potential pollutants, making it difficult to treat and inefficient in resource utilization. This work developed a new multifunctional evaporator that integrated photothermal evaporation with photocatalytic degradation to enable in situ purification and water recovery. The device was fabricated by immobilizing a hydrothermally synthesized Nickel Oxide- Titanium Dioxide- Carbon Nanotube (NiO-TiO₂-CNT) composite on a melamine sponge via sodium alginate crosslinking, forming a synergistic evaporation–degradation interface. Under 1 kW·m⁻² solar irradiation, the system achieved an evaporation rate of 2.25 kg·m⁻²·h⁻¹ , where the addition of 1 mmol·L⁻¹ PMS boosted phenol removal efficiency to 90 %. When applied to real LFL, COD was reduced from 4266 to 53 mg·L⁻¹ , and phosphate was completely removed to below detection limits (<0.01 mg·L⁻¹). Although ammoniacal nitrogen levels remained relatively high at 354 mg·L⁻¹ , post treatment phytotoxicity tests involving irrigation of wheat with leachate resulted in a 90 % germination rate, which increased to 100 % after dilution. This indicated the treated leachate was safe and could potentially be used as a nitrogen-rich liquid fertilizer. A techno-economic analysis showed that the system could be operated at a cost of only 10.33 USD per metric ton, significantly lower than conventional treatments. This work thus provides a cost-effective sustainable strategy for simultaneous pollutant control, water reuse, and ecological restoration of landfill sites.
研究领域:
环境科学与技术
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水处理
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太阳能利用
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高级氧化工艺
·
可持续资源回收
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）