ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 415
·期 3
·493-507
·
IF 4.478
Li Zhuoya,Huang Xinyi,Wang Xuan,Ren Jianwei,Xu Buyi,Luo Chunying,Wang Yanjun,Wang Lian,Harada Kouji H.,Zou Xiaoli
A rapid and sensitive high-performance liquid chromatography–high-resolution orbitrap mass spectrometry method was developed for the simultaneous screening of 354 organic poisons and metabolites in blood and urine, including drugs, medications, pesticides, rodenticides, veterinary drugs, alkaloids, and mycotoxins with a multi-toxicant chromatography-mass spectrometry information library. The method and library showed good prospects in clinical poisoning screening and forensic toxicological identification. Blood and urine samples were extracted successively with ethyl acetate in acidic and alkaline conditions; then, the extract was blown to nearly dry by nitrogen gas and redissolved with methanol-aqueous solution (v:v, 50:50), and the dissolved solution was analyzed by LC-MS/MS after filtering. Precursor ions’ m / z was set for identification, retention time, fragment ions, and isotopic pattern which were used for confirmation. No interference peaks were found in the blank samples, showing good specificity. The LODs of toxicants in urine and blood were 1.00×10 −3 –50.0 ng/mL and 2.07×10 −3 –50.0 ng/mL, respectively, while the LOQs were 3.30×10 −3 –1.67×10 2 ng/mL and 6.91×10 −3 –1.67×10 2 ng/mL. The intra-day precision and inter-day precision of urine samples were 2.31–9.13% and 4.75–12.3%, respectively, which were 1.92–10.8% and 2.01–12.1% in blood samples. The established method was applied to analyze 9 cases of clinical poisoning patients, and bromadiolone, carbofuran, and amanitins were detected, respectively. A total of 382 biospecimens from drug abusers were analyzed with the proposed method, which indicated that some drugs were detected in 62 cases, mainly including methamphetamine, heroin, and MDMA. The results were consistent with the information from traditional liquid chromatography-triple quadrupole mass spectrometry. Graphical abstract
研究领域:
临床医学
·
法医学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Water Process Engineering
·
2024
·
IF 6.3
Pengkun Zhang,Haoyu Wang,Yujie Lai,Yunzhuo Xu,Longyan Chen,Qinhan Wu,Dong-Hau Kuo,Dongfang Lu,Mengistu Tadesse Mosisa,Jiqing Li,Jinguo Lin,Xiaoyun Chen
The rising accumulation of pollutants, including heavy metals and toxic organics, necessitates effective environmental remediation to protect health and biological systems. In this study, a cubic cerianite CeO 2 -like Co/S co-doped CeO 2 sulfur-oxide catalyst (labeled as CeCoOS) with super catalytic reduction performance of pollutants with NaBH 4 as a reducing agent under dark was prepared via a green and facile preparation method. Transition metal Co 2+ -cation and S 2− -anion co-doped CeO 2 adjusted the energy bandgap and introduced oxygen vacancy (Vo), which converted Ce 4+ around Vo into Ce 3+ to maintain electric neutrality. The heterovalent Ce 4+ /Ce 3+ states in Co/S-CeO 2 enhance the electron lifetime and facilitate fast electron transfer . The valence transition between Ce 4+ /Ce 3+ and Vo make the Co/S co-doped in CeO 2 catalyst increase its electrochemically active surface sites and accelerate the pollutants reduction reaction. The CeCoOS-3 with Co/S dopants has an excellent catalytic reduction activity to completely reduce 20 ppm toxic 4-NP in 10 min, 50 ppm organic dyes of MB, RhB, and MO in 6 min, and 50 ppm heavy metal Cr 6+ in 10 min. The CeCoOS-3 sulfur-oxide catalyst also exhibited excellent stability in the reusability test. Therefore, CeCoOS sulfur-oxide catalysts are expected to be used for dye decolorization, detoxification of organic toxins, and reduction of electroplating wastewater.
研究领域:
环境科学
·
催化化学
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL TECHNOLOGY
·
2015
·
IF 1.56
Zonghe Zhang,Ping Zheng,Wei Li,Ru Wang,Abbas Ghulam
Denitrification plays a key role in the biological nitrogen removal from the wastewater using granular sludge as the integral part of a high-rate denitrification technology. It is helpful to evaluate the effect of typical organic toxicants on the activity of denitrifying granular sludge for the application of denitrification technology. In this study, four typical organic toxicants, namely, penicillin, chloramphenicol, 2,4-dinitrophenol and polymyxin B sulphate were used to assess the effect of organic toxicants on the activity of denitrifying granular sludge. The results of individual toxicity indicated that penicillin, chloramphenicol and 2,4-dinitrophenol had significant inhibition, whose half-inhibitory concentrations were 0.534, 0.162 and 0.474 g/L with respective inhibitory magnitudes of 90.79%/(g/L), 282.5%/(g/L) and 138.83%/(g/L). Polymyxin B sulphate showed no significant inhibition. The results of combined toxicity indicated that the binary mixture of penicillin and chloramphenicol had an antagonistic effect, both the binary mixture of penicillin and 2,4-dinitrophenol and the binary mixture of chloramphenicol and 2,4-dinitrophenol had additive effects. The ternary mixture of penicillin, chloramphenicol and 2,4-dinitrophenol had a partial additive effect.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2019
·Vol. 103
·期 8
·3581-3595
·
IF 3.67
Li Yunlong,Wang Baoying,Chang Yafeng,Yang Yuting,Yao Chunzhi,Huang Xinqi,Zhang Jinbo,Cai Zucong,Zhao Jun
Replant failure has threatened the production of Sanqi ginseng ( Panax notoginseng ) mainly due to the accumulation of soil-borne pathogens and allelochemicals. Reductive soil disinfestation (RSD) is an effective practice used to eliminate soil-borne pathogens; however, the potential impact of RSD on the degradation of allelochemicals and the growth of replant Sanqi ginseng seedlings remain poorly understood. In this study, RSD was conducted on a Sanqi ginseng monoculture system (SGMS) and a maize-Sanqi ginseng system (MSGS), defined as SGMS_RSD and MSGS_RSD, respectively. The aim was to investigate the impact of RSD on allelochemicals, soil microbiomes, and survival rates of replant seedlings. Both short-term maize planting and RSD treatment significantly degraded the ginsenosides in Sanqi ginseng–cultivated soils, with the degradation rate being higher in the RSD treatment. The population of Fusarium oxysporum and the relative abundance of genus Fusarium were dramatically suppressed by RSD treatment. Furthermore, the RSD treatment, but not maize planting, markedly alleviated the replant failure of Sanqi ginseng, with the seedling survival rate being 52.7–70.7% 6 months after transplanting. Interestingly, RSD followed by short-term maize planting promoted microbial activity restoration, ginsenoside degradation, and ultimately alleviated the replant failure much better than RSD treatment alone (70.7% vs. 52.7%). Collectively, these results indicate that RSD treatment could considerably reduce the obstacles and might also act as a potential agriculture regime for overcoming the replant failure of Sanqi ginseng. Additional practices, such as crop rotation, beneficial microorganism inoculation, etc. may also still be needed to ensure the long-term efficacy of seedling survival.
研究领域:
植物学
·
农业科学
·
微生物学
✓ 已验证
DOI
高影响因子
Applied Sciences-Basel
·
2019
·Vol. 9
·期 5
·935
·
IF 2.217
Jing Neng,Chen Xiang,Kan Jia,Xiaohua Nie,Peilong Sun
Featured ApplicationThe prepared hydrophobic gold nanomaterials show excellent surface-enhanced Raman scattering activity and, therefore, could be used as efficient organic-soluble SERS substrates for the detection of many hydrophobic food toxicants, such as 3,4-benzopyrene, and carcinogens, such as benzidine.AbstractMany previously reported syntheses of gold nanoparticles required lengthy reaction times, complicated operations, high temperatures, or multi-step manipulations. In this work, a morphology-controlled versatile one-pot synthesis of hydrophobic gold nanodots, nanobars, nanorods, and nanowires has been developed. A series of gold nanomaterials ranging from round nanodots, short nanobars, and long nanorods to ultrathin and ultralong nanowires (diameter <2 nm, length >2 μm) have been readily prepared by simply adjusting the feeding ratio of chloroauric acid to oleylamine, oleic acid, and triphenylsilane. The silk-like ultralong and ultrathin nanowires were found to have a single crystalline structure and may have significant potential applications in microelectronics and biosensors. Large sizes of gold spherical nanoparticles were obtained from gold nanodots via a seed-mediated growth approach. These nanoparticles and ultralong nanowires showed excellent surface-enhanced Raman scattering (SERS) activity in organic solvents and, therefore, were employed as efficient organic-soluble SERS substrates for the detection of hydrophobic food toxicants, such as 3,4-benzopyrene, and carcinogens, such as benzidine.Keywords:synthesis;gold nanodots;nanobars;nanorods;nanowires;surface-enhanced Raman spectroscopy
研究领域:
纳米技术
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Forensic Toxicology
·
2023
·Vol. 41
·期 2
·241-248
·
IF 2.2
Guo Caixia,Yan Hui,Liu Wei,Xiang Ping,Di Bin,Shen Min
Purpose An analytical method using liquid chromatography with tandem mass spectrometry (LC–MS/MS) was established and validated for screening 425 drugs and poisons in dried blood spots (DBSs). Methods Blood (20 μL) was spotted on Whatman FTA™ classic card to prepare DBS sample, then extracted with 150 μL methanol and analyzed by LC–MS/MS using a multiple reaction monitoring method. Results The limit of detection of the compounds were 0.1–10 ng/mL. The values for recovery and matrix effect were 40.3–114.9% and 40.2–118.4%, respectively. This method was successfully applied to DBS samples from 105 humans suspected of drug poisoning, which was stored for 3–5 years at room temperature. Thirty-three kinds of drugs, including benzodiazepines, antipsychotics, antidepressants, antipyretic analgesics, non-steroidal anti-inflammatory drugs, antibiotics, antiepileptic drugs, new psychoactive drugs were confirmed in 102 cases, while no compound was detected in the other 3 cases. Estazolam, a benzodiazepine widely used in clinical practice as a sedative, hypnotic, and anti-anxiety drug, was the most frequently detected substance, occurring in 34.2% of the cases. Conclusions Most drugs in DBS could still be detected after storage for 3–5 years, but ambroxol, zopiclone, carbofuran, chlorpyrifos, and valproic acid were not detectable after 3–5 years of storage at room temperature. The components measured in DBS were consistent with those measured in whole blood at the collection time, thereby confirming that DBS samples have the advantage of stable storage at room temperature.
研究领域:
分析化学
·
法医学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2021
·
IF 5.909
Leila Roshanfekr Rad,Mansoor Anbia
The adsorption of toxic matters from water using zeolites has been developed due to the low cost of operation and adsorbent. However, their use due to the lower efficiency of natural zeolites and the difficult reusability of nanosized-synthetic zeolites is limited. In recent twenty years, the use of zeolite-based composites in the forms of zeolite/inorganic and zeolite/polymer composites has been accelerated for the adsorption of toxic matters from water sources. This review summarized the recent studies on the capability of zeolite-based composites toward toxic matters adsorption such as heavy metal ions , dyes, herbicides, and drugs from water. The main adsorption mechanisms using the zeolite-based composites are surface adsorption, chelation, ion exchange, electrostatic interaction, diffusion and, complexation. The comparison of the capability of zeolite-based composites and raw zeolites for the removal of toxic matters from the water provides the valuable applicability of the zeolite-based composites for actual wastewater treatment in the future.
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2019
·
IF 5.714
Hui Huang,Chao Wang,Shuchang Liu,Zehao Sun,Dejun Zhang,Caicai Liu,Yang Jiang,Shuyue Zhan,Haofei Zhang,Ren Xu
The automatic identification (location, segmentation , and classification) by UAV- based optical imaging of spills of transparent floating Hazardous and Noxious Substances (HNS) benefits the on-site response to spill incidents, but it is also challenging. With a focus on the on-site optical imaging of HNS, this study explores the potential of single spectral imaging for HNS identification using the Faster R-CNN architecture. Images at 365 nm (narrow UV band), blue channel images (visible broadband of ∼400–600 nm), and RGB images of typical HNS (benzene, xylene, and palm oil) in different scenarios were studied with and without Faster R-CNN. Faster R-CNN was applied to locate and classify the HNS spills. The segmentation using Faster R-CNN-based methods and the original masking methods, including Otsu, Max entropy, and the local fuzzy thresholding method (LFTM), were investigated to explore the optimal wavelength and corresponding image processing method for the optical imaging of HNS. We also compared the classification and segmentation results of this study with our previously published studies on multispectral and whole spectral images. The results demonstrated that single spectral UV imaging at 365 nm combined with Faster R-CNN has great potential for the automatic identification of transparent HNS floating on the surface of the water. RGB images and images using Faster R-CNN in the blue channel are capable of HNS segmentation.
研究领域:
环境科学
·
图像处理
·
遥感技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2017
·
IF 1.15
Xiuping He,Jiangtao Wang,Xiaoqi Mei
A multitemplate molecularly imprinted polymer was synthesized using fragments similar to gonyautoxins 1 and 4 including 2,4,6-triaminopyrimidine, 4-hydroxy-2-butanone, and imidazole as dummy templates by bulk polymerization. Scanning electron microscopy and infrared spectroscopy showed a porous structure and the formation of organic groups in the imprinting process of the polymer. One millilitre of 0.1 mol L−1 acetic acid and 1 mL of 95% methanol were the optimized washing and eluting solutions during the molecularly imprinted solid-phase extraction. The adsorption capacity of the multitemplate molecularly imprinted polymer was lower than for the monotemplate molecularly imprinted polymer prepared in a previous study in seawater in which Alexandrium tamarense and Alexandrium minutum were cultivated. This result may due to the inappropriate combination of the dummy template fragments in the synthesis resulting in the unfitness of the imprinting sites for gonyautoxins 1 and 4. In general, it is inappropriate to use fragments with the similar parts to the analyte as the dummy templates. However, this work indicated the optimal molecularly imprinted polymer for the selective extraction of gonyautoxins 1 and 4.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF APPLIED PHYCOLOGY
·
2023
·Vol. 35
·期 5
·2219-2239
·
IF 3.3
Gan Tingting,Yin Gaofang,Zhao Nanjing,Tan Xiaoxuan,Wang Ying,Sheng Ruoyu,Ye Ziqi
Microalgal fluorescence kinetics method, as a promising technique for rapidly detecting water toxicity, still lacks a consistent response index with sensitive response characteristics to different types of toxic pollutants. In order to solve this problem, in this study, the influences of six typical toxic pollutants belonging to different types, including the heavy metal cadmium, the herbicide atrazine, organic chemical raw materials 1,4-benzoquinone and 1,4-dichlorobenzene, and the disinfection by-products chloroacetic acid and trichloroacetonitrile on the fluorescence rise kinetics (OJIP) curve of microalga Chlorella pyrenoidosa were investigated. According to the same characteristics of changes in the OJIP curves of different toxic pollutant treatments, a new response index for detecting the comprehensive toxicity of water (RI ct ) was constructed. By comparing with the maximum photochemical quantum yield of photosystem II (PSII) (F v /F m ), the potential quantum yield of PSII (F v /F o ), the performance index of PSII (PI ABS ) and the quantum yield of electron transport of PSII (φ Eo ), the feasibility and sensitivity of quantitative response of RI ct to the toxicity of each pollutant and polluted water containing multiple pollutants were verified. The results indicated that all the six toxic pollutants had the same influence on parameters F v(m-j) /F o and F v(m–o) /F o contained in the OJIP curve, and RI ct constructed based on F v(m-j) /F o and F v(m–o) /F o had a good logistic curve concentration–response relationship with each pollutant. By comparison, the response sensitivity of RI ct to all pollutants was significantly superior to F v /F m , F v /F o and φ Eo , and also had better stability in the toxicity response to each pollutant compared to PI ABS , indicating that RI ct was a suitable response index for rapidly and sensitively detecting water comprehensive toxicity based on microalgal fluorescence kinetics. This study provides an important basis for the development of rapid and sensitive detection methods for water toxicity.
研究领域:
环境科学
·
生物学
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2021
·
IF 9.028
Kun Zhang,Jiahui Liang,Nadja R. Brun,Yanbin Zhao,Andreas A. Werdich
Rapid and cost-effective in vivo assays to screen potential environmental neurodevelopmental toxicants are necessary to address the limitations of in vitro platforms, such as the inability to fully recapitulate the developmental and physiological processes of whole organisms. In the present study, a rapid zebrafish behavioral profiling assay was developed to characterize the neurodevelopmental effects of environmental substances by quantitatively evaluating multiple spontaneous movement features of zebrafish embryos. This video analysis-based assay automatically segmented every embryo and thus was able to accurately quantify spontaneous movement features, including frequency, duration, intensity, interval, and the number of continuous movements. When tested with eight environmental substances known to be neurodevelopmental toxicants, such as chlorpyrifos and bisphenol A, the assay successfully captured frequency alterations that were well-documented in previous studies while also providing additional information. Using an optimized procedure, we further assessed 132 potential neurotoxins that spanned a wide range of molecular targets, many of which were previously detected in environmental waterbodies. The distinct altered behavioral barcodes indicated that the spontaneous movement was impacted by diverse neuroactive substances, and the effects could be effectively evaluated with the developed assay. The web-based tool, named EMAnalysis, is further provided at http://www.envh.sjtu.edu.cn/zebrafish_contraction.jsp. Thus, this assay provides an efficient platform to accelerate the pace of neurotoxic environmental contaminant discoveries.
研究领域:
环境科学
·
神经科学
·
毒理学
·
发育生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Polymer Materials
·
2022
·
IF 4.855
Jinsong Zeng,Tianguang Wang,Zheng Cheng,Lu Liu,Fugang Hu
A cellulose nanofiber-SiO2 hybrid aerogel cigarette (CSG) filter, which can be used for ultrahigh adsorption of toxic substances from cigarette smoke, was prepared based on the skeletal material of cellulose nanofibers (CNFs) with the help of a simple directional frozen technology. SEM and FTIR results confirmed the successful introduction of the SiO2 nanoparticles, and the application process of SiO2 nanoparticles did not influence the three-dimensional network structure of CSGs. Considering the practical application of the CSGs on the cigarette filter, the mechanical and thermal properties of the CSGs were investigated. The stress–strain and TG curves showed that the mechanical and thermal stability properties of the CSGs increased compared with the pure cellulose nanofiber aerogel without the introduction of SiO2 nanoparticles. The adsorption capacity of the CSG filter tips against toxic substances from cigarette smoke was systematically investigated. Compared with conventional cellulose acetate filter tips, the CSG filter tips performed well at adsorption of tar, TPM (total particulate matter), nicotine, and CO from cigarette smoke with removal efficiencies of 92.23, 90.25, 95.02, and 20.63%, respectively. Crucially, the preparation of CSGs needs no extra chemical agents, showing an eco-friendly process. These results suggest that CSGs display great potential working as an ideal candidate for mainstream smoke toxicant adsorption.
研究领域:
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）