FOOD CONTROL
·
2015
·
IF 2.806
Zhiyong Zhao,Na Liu,Lingchen Yang,Jianhua Wang,Suquan Song,Dongxia Nie,Xianli Yang,Jiafa Hou,Aibo Wu
The primary objective of this study was to synthesize three types of cross-linked chitosan polymers and further investigate their adsorption capability for multiple mycotoxins , including aflatoxin B 1 (AFB 1 ), ochratoxin A (OTA), zearalenone (ZEN), fumonisin B 1 (FB 1 ), deoxynivalenol (DON) and T-2 toxin (T2). Among these synthetic adsorbents, cross-linked chitosan-glutaraldehyde complex presented the highest adsorption capability for AFB 1 (73%), OTA (97%), ZEN (94%) and FB 1 (99%), but no obvious adsorption for DON and T2 (<30%). The effect of various incubation conditions (contact time, dosage and pH) was also studied. Subsequently, the experimental data were fitted to Langmuir, Freundlich and Hill models. The best fitting model to describe AFB 1 and FB 1 adsorption was Langmuir model ( R 2 ≥ 0.99), with the theoretical maximum adsorption amounts of 5.67 mg/g for AFB 1 and 15.7 mg/g for FB 1 . The Hill model was the best model for OTA and ZEN adsorption ( R 2 > 0.98), with the predicted maximum adsorption amounts were 24.8 mg/g for OTA and 9.18 mg/g for ZEN. In addition, the adsorption capability of adsorbent for the simultaneous presence of multiple mycotoxins was also evaluated in buffer system and simulated gastrointestinal condition. The results indicated that the coexisted multiple mycotoxins didn't affected the adsorption capability of adsorbent, whereas the adsorption amounts of toxins were decreased by some gastrointestinal components. The findings of this research suggest that chitosan–glutaraldehyde complex has the potential to be applied as multitoxin adsorbent material for reducing the combined adverse effect of multiple mycotoxins on humans and animals.
研究领域:
食品科学
·
毒理学
·
生物化学
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产品关联
DOI
高影响因子
CELLULOSE
·
2025
·
IF 4.8
Bai Xiaoyu,Wan Mengyu,Fan Wuhou,Kang Jianping,Xia Zhaoxia,Li Jiangtao,Lan Jingying,Xiao Lan,Zuo Song,Jiang Yuanzhang,Tan Lin,Zhang Yong
Electrostatic spinning technology can prepare nanofiber materials with high specific surface area and unique microstructure. Cellulose acetate (CA) has excellent membrane formation and processability, while chitosan (CS) is positively charged due to the abundance of amino groups and has certain biological activities. In this paper, CA/CS nanofiber membranes with different CS contents were prepared by solution blending and electrostatic spinning techniques and used in hemoperfusion (HP) for targeted removal of endotoxins. The CA/CS nanofibrous membranes were systematically characterized using infrared spectroscopy, scanning electron microscopy, etc., and the clearance ability of CA/CS nanofibrous membranes for endotoxin was tested under static and dynamic simulated HP conditions. The results showed that the addition of CS changed the structure of CA nanofibers, significantly increased the specific surface area of CA/CS nanofiber membrane, and provided more adsorption sites for the membrane material. The positive charge of CS interacted with the negatively charged endotoxin in the blood through electrostatic attraction, which enabled the CA/CS nanofiber membranes to adsorb endotoxin efficiently and exhibit higher endotoxin clearance ability. In the static adsorption experiment, when the CS content was 3.75%, the endotoxin adsorption and clearance rate could reach 580.0 ± 32.2 EU/g and 65.1 ± 3.6%, respectively. In the dynamic adsorption experiment, when the CS content was 2.50%, the CA/CS nanofiber membrane had a good effect on the adsorption of endotoxin. Meanwhile, CA/CS nanofiber membrane also has good blood compatibility. Therefore, the CA/CS nanofiber membrane is potentially valuable in blood purification.
研究领域:
生物材料学
·
医学工程
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产品关联
DOI
高影响因子
Artificial Cells Nanomedicine and Biotechnology
·
2018
·
IF 3.026
Wenhui Zong,Jian Chen,Wenyan Han,Guanghui Cheng,Jie Chen,Yue Wang,Weichao Wang,Lailiang Ou,Yaoting Yu,Jie Shen
A novel polyvinyl alcohol-amino multi-walled carbon nanotube (PVA-AMWCNT) nanocomposite microsphere was prepared successfully for the first time and used for endotoxin removal. The resulting AMWCNT modified PVA microsphere was characterized by SEM, Raman spectrum and fluorescence image, which indicated AMWCNT was dispersed into the macropores of PVA microsphere uniformly. The PVA-AMWCNT microspheres showed better adsorption capability and faster adsorption equilibrium for endotoxin in aqueous solution when compared to the PVA microsphere with polymyxin B (PMB) as ligand. More noteworthy, the PVA based microspheres had little nonspecific adsorption in simulated serum. Therefore, PVA-AMWCNT nanocomposite microsphere with an excellent haemocompatibility has a great potential application in clinical blood purification.
研究领域:
材料科学
·
生物医学工程
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION SCIENCE AND TECHNOLOGY
·
2012
·
IF 1.088
Yu Yi,Canlin Lai,Yuqi Jiang,Wenyue Xiong,Jianfeng Mei,Guoqing Ying
A novel magnetic chitosan microsphere (MCM) was prepared from chitosan and Fe3O4 through inverse suspension crosslinking, epichlorohydrin activation, and grafted with ligands. The magnetometer measurements data indicated that the magnetic microspheres had superparamagnetic property as well as fast magnetic response without the magnetic hysteresis effect and thus can be well used in magnetic separation. The adsorption results showed that the magnetic chitosan microsphere grafted with ligand had good adsorption capacity on endotoxin up to 554.1 EU/g under the condition of 25.0 mg/mL adsorbent, 17.2 EU/mL endotoxin. It is expected that this new microsphere will have a feasible and useful application in adsorbing endotoxin from biological products.
研究领域:
生物医学工程
·
材料科学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 64
·51631-51641
·
IF 3.84
Rui Wang,Xin Jiang,Ai He,Tao Xiang,Changsheng Zhao
The application of chitosan (CS) as adsorbent was limited by its poor acidic resistance, solubility in organic solvents and miscibility with synthetic polymers. In this study, an effective way was utilized to increase the amount of CS in hybrid particles together with good acid–alkali resistance and mechanical strength. CS was chemically modified and in situ cross-linked in polymer solutions to prepare hybrid particles with a semi-interpenetrating network (semi-IPN) structure. Polyethersulfone (PES) was chosen as a representative polymer matrix to prepare hybrid particles; and the PES/CS-derivative particles exhibited good adsorption capacity to the toxin bilirubin without procoagulant activity and adsorption capacity to Cu2+. Furthermore, the PES/CS-derivative hybrid particles exhibited selective adsorption to the anionic dye Congo Red (CR) and the environmental hormone bisphenol A (BPA) compared with other dyes or phenols. The results indicated the hybrid particles had versatile applications in blood purification and wastewater treatment.
研究领域:
材料科学
·
生物医学工程
·
环境科学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Tingting Xu,Huanli Gao,Jiahuan Zhou,Ming He,Xingxiang Ji,Hongqi Dai,Orlando J. Rojas
There is an urgent need to develop materials to prevent bacterial infection and the deleterious effects of endotoxins. In this study, we introduce a one-step electrodeposition method to prepare films composed of chitosan/Ag/polypyrrole and layer-by-layer self-assembly to introduce lignin sulphonate (LS) to obtain chitosan/Ag/polypyrrole/LS films. Antibacterial effects against both E. coli and S. aureus are shown by bacterial growth profiles and observation of bacteriostatic zones. Meanwhile, the addition of self-assembled LS improved the antibacterial effect of the film. For E. coli , the inhibition zone diameter was 0.93 cm, while for S. aureus , the inhibition zone diameter was 0.72 cm. Rapid and efficient endotoxin adsorption effects were shown whereby the electrostatic interactions between chitosan and endotoxin molecules played a major role. After adsorption for 1 h, in initial concentration of 1 EU/mL endotoxin solution, the adsorption efficiency could reach up to 85 %, while in initial concentration of 5 EU/mL endotoxin solution, the adsorption efficiency could reach up to 87.6 %. The results suggest chitosan/Ag/polypyrrole/LS films for their capability as a new type of antibacterial film with intrinsic endotoxin adsorption activity.
研究领域:
生物材料学
·
医学工程
·
微生物学
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产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2025
·
IF 9.6
Liqin Wu,Xi Luo,Pengpeng Yue,Kalibinuer Yasen,Zibiao Zhong,Shuangquan Wu,Qifa Ye
The accumulation of uremic toxins, which ultimately leads to renal failure, results in systemic complications and necessitates efficient and biocompatible removal strategies. This paper presents the preparation of a biomimetic anisotropic aerogel composed of polyvinyl alcohol (PVA), sodium alginate (SA), and MXenes (Ti 3 C 2 T X ) via directional freezing. Inspired by plant vascular bundles, the resulting vertically aligned porous structure enhances the mechanical stability of and mass transfer in the material. The aerogel with its high MXene loading and high surface utilization offers remarkable adsorption capacities for urea (221.77 mg g −1 ) and creatinine (138.07 mg g −1 ). In vitro assays confirm the excellent hemo- and cytocompatibility of the aerogel. In a rabbit model of acute ischemic kidney injury, two-hour hemoperfusion with the aerogel significantly lowers the urea (38.67 to 16.17 mmol L −1 ) and creatinine levels (744.62 to 307.46 µmol L −1 ) without observable toxicity. The multidimensional biomimetic multipore anisotropic MXene aerogel presented here is a structurally optimized and biologically safe adsorbent for efficient uremic toxin clearance and a promising alternative to conventional hemodialysis.
研究领域:
生物材料
·
肾病学
·
组织工程
·
纳米医学
·
再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2024
·
IF 2.7
Yuan Zhou,Qiang Zhang,Zhaoxia Xia,Zhaojiang Wang,Libin Liu,Yuanzhang Jiang,Yanting Han,Linshen Xie,Lin Tan,Yong Zhang
Blending modification is used to improve the hemocompatibility of cellulose acetate (CA) dialysis membranes while endowing the CA membrane with endotoxin scavenging and antibacterial capabilities. First, polydopamine (PDA) and polyethylenimine (PEI) are used as negatively charged and positively charged moieties, respectively, together with cellulose nanocrystals (CNCs) to construct mixed charge CNCs (PEI-PDA@CNCs). Then PEI-PDA@CNCs is blended with the CA casting solution, and the modified CA dialysis membrane is prepared through a phase inversion process. Through biocompatibility, adsorption, antibacterial, and filtration experiments, various properties of PEI-PDA@CNCs modified CA membrane (PDCA) are systematically tested. The results show that the PDCA membrane exhibited good blood compatibility and noncytotoxicity. At the same time, the PDCA membrane has antibacterial properties and excellent endotoxin adsorption capacity. Compared with the CA membrane, the clearance rates of PDCA membrane for Staphylococcus aureus and Escherichia coli reach 54.0% ± 1.5% and 52.9% ± 5.9% respectively. In the dynamic adsorption experiment, the adsorption capacity of PDCA membrane for endotoxin increases to 2322.1 ± 45.9 EU/g. The research results indicate that PEI-PDA@CNC-modified multifunctional CA membrane with endotoxin adsorption and antibacterial properties has potential application prospects in the fields of hemodialysis and blood purification.
研究领域:
材料科学
·
生物医学工程
·
纳米技术
·
血液透析
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2012
·
IF 1.289
Yu Yi,Canlin Lai,Yuqi Jiang,Jianfeng Mei,Hong Wang,Guoqing Ying
In this article, we present a novel amino-reserved magnetic chitosan microsphere (ARMCM) prepared from chitosan and Fe 3 O 4 through inverse suspension crosslinking by formaldehyde and epichlorohydrin. The density of ARMCM was 1.47 g/mL, and the amino content was 2.44 mmol/g. The measurements data indicated that the saturation magnetization (σ s ) of ARMCM was 23.2 emu/g. The adsorption results showed that these magnetic chitosan microspheres had good adsorption capacity on endotoxin: the maximum absorption capacity was about 1792.1EU/g in 40 min; the endotoxin adsorption efficiency in the protein solution was close to 80% and what is more, protein adsorption efficiency was only 23.2 and 2.0%, respectively, for acidic and basic proteins. It is expected that this ARMCM will have a feasible and useful application in adsorbing endotoxin from biological products. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
研究领域:
材料科学
·
生物技术
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2022
·
IF 10.53
Weiyu Dou,Feng Qi,Yangyang Li,Fangfang Wei,Qitao Hu,Zhikan Yao,Jing Wang,Lin Zhang,Zhe Tang
Endotoxin has been recognized as the crucial inducer for sepsis, and selective removal of endotoxin from plasma in vitro is the popular therapeutic strategy, such as hemoperfusion technology based on adsorption. Construction of hemoperfusion adsorbents with high endotoxin adsorption capacity in plasma is critical to meet the demands of hemoperfusion therapy for sepsis. However, it has proven to be challenging because of the adhesion of plasma protein. Inspired by the antiadhesion property of mixed-charge polymers and the endotoxin adsorption property of polyethyleneimine , here, a charge-bias, endotoxin selectively adsorbable and hemocompatible layer was constructed on nanofibrous surface of polysulfone (PSF) electrospun nanofibrous membrane (ENM) by the co-deposition of positively-charged polyethyleneimine (PEI) and negatively-charged polydopamine (PDA). The charge-bias degree of PEI/PDA co-deposition layer was quantified through zeta potential measurement, and firstly visualized through Kelvin probe force microscopy (KPFM) measurement. The continuity and integrity of PEI/PDA layer were characterized by XPS and TEM . We showed that the light positive charge-bias of the PEI/PDA layer in physiological environment (pH = 7.4) can macroscopically drove the adsorption selectivity towards endotoxins via electrostatic interaction , and uniformly distributed positive and negative charges can microscopically drove the anti-protein adhesion property. The resultant PEI/ [email protected] ENM exhibited high adsorption capacity of 9049 EU/g in plasma, which is much higher than commercial endotoxin adsorbents. Notably, PEI/ [email protected] ENM possessed satisfactory anti-plasma protein adhesion property, hemocompatibility and had no adverse effects on blood components, especially plasma protein. This work suggests a feasible approach for producing ENMs-based hemoperfusion adsorbent matrix for endotoxin-induced sepsis.
研究领域:
生物材料
·
生物医学工程
·
纳米技术
·
医学科学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Hongyu Yin,Zhoujun Wang,Xiang Zhang,Weifeng Zhao,Ran Wei,Changsheng Zhao
Chronic liver failure (CLF) and chronic renal failure (CRF) lead to toxins accumulation, severely impairing organ functions. To address this challenge, a Janus polyethersulfone (PES)-based architecture integrated dense membrane with porous monolith is developed, pioneering an approach for simultaneous plasma separation and toxins adsorption. The porous monolith, featuring high porosity about 83.3 % and substantial specific surface area about 102.06 m 2 /g, is prepared using the freezing-induced phase separation (FIPS) technique. The porous monolith is composed of polymer networks of PES and amphiphilic copolymer of poly (vinyl pyrrolidone-co-methyl methacrylate) (VM), as well as activated carbon. Among the various monolith, the PES 18 VM 5 C 2 exhibits the optimal balance of adsorption and anti-protein adhesion. The PES-VM-incorporated dense membrane with a controlled submicron pore size is constructed atop the PES 18 VM 5 C 2 . The dense membrane effectively blocks hemocytes and the porous monolith efficiently adsorbs toxins. Together, the Janus architecture PES 18 VM 5 C 2 @M 16 allows over 85 % of bovine serum albumin (BSA) to permeate, showcasing its selective permeability. After being integrated into a custom 3D-printed supporting device, the PES 18 VM 5 C 2 @M 16 achieves significant clearance in creatinine (67.6 %), uric acid (87.4 %), and bilirubin (89.1 %) during 2-hour plasma adsorption test, with minimal impact on essential plasma components such as total protein, albumin, and cholesterol. This work presents a “Janus Interface Architecture” as next-generation platform for plasma separation and toxins adsorption, offering a promising strategy for wearable artificial liver systems.
研究领域:
生物医学工程
·
材料科学
·
血液透析
·
血浆分离技术
✓ 已验证
产品关联
DOI
高影响因子
EUROPEAN POLYMER JOURNAL
·
2018
·
IF 3.741
Yao Huang,Zhipeng Yuan,Dan Zhao,Fang Wang,Kexin Zhang,Yansheng Li,Yongqiang Wen,Changtao Wang
Nanofiber sponge, which showed high porosity and excellent blood compatibility, was successfully prepared by electrospinning and freeze-drying technology. The APTT (activated partial thromboplastin time) and PT (prothrombin time) of human plasma with nanofiber sponge were a little longer than control groups, which revealed the nanofiber sponge has excellent blood compatibility. By activating the carboxyl groups on the fibers, PMB (polymyxin B) was successfully immobilized on the nanofiber sponge. The adsorption performance was researched in different solutions. The adsorption capacity in physiological saline solution was around 17,889 EU/mL and the adsorption equilibrium time was about 40 min. The adsorption behavior not only obeyed to second-order kinetic equation, but also was conformed to Langmuir model, which turned out to be chemical adsorption and monolayer adsorption. The endotoxin removal rate in BSA (bovine serum albumin) physiological saline solution was about 96.6%, and the BSA recovery rate was around 75.6%, which indicated the PMB immobilized nanofiber sponge adsorbed endotoxin selectively. The endotoxin removal rate of normal nanofiber sponge was about 17.3%, while it was around 99.6% when it comes to PMB immobilized-nanofiber sponge, which manifested PMB played an important role in endotoxin adsorption. The endotoxin removal rate in human plasma reached 90%, and the adsorption reached equilibrium within 60 min, which proved the PMB immobilized-nanofiber sponge would have great potential for applying in clinical blood purification.
研究领域:
生物医学工程
·
材料科学
·
药学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）