FOOD AND CHEMICAL TOXICOLOGY
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2014
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IF 2.61
Lingchen Yang,Zhiyong Zhao,Yifeng Deng,Zhenlei Zhou,Jiafa Hou
The T-2 and HT-2 toxins, the main metabolites of Fusarium poae , induce toxicity in broilers and accumulate in tissues. Consequently, during the breeding process of broilers, diets are frequently supplemented with physical adsorbents to protect birds against the toxicity induced by mycotoxins. In the present research, T-2 and HT-2 were produced in maize inoculated with F. poae . Mont, the strongest adsorbent based on in vitro adsorption ratios, was added to the contaminated diet. One-day-old chickens were randomly and equally divided into the following four groups: control diet group, Mont supplemented diet group, contaminated diet group and detoxification diet group. The experiment lasted for 42 days. Compared to the control group, the contaminated group showed significant decrease in body weight, feed intake and TP ( P < 0.05), and marked increase in FCR, ALP, AST and ALT activity, T-2/HT-2 residues in the tissues and the relative expressions of apoptosis-related mRNAs ( P < 0.05). Mont supplementation provided protection for the treated broilers in terms of performance, blood biochemistry, hepatic function, T-2/HT-2 residue of tissues and apoptosis. Therefore, Mont may be suitable as a detoxification agent for T-2/HT-2 in feed for broilers.
研究领域:
动物科学
·
毒理学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2015
·
IF 2.895
Lingchen Yang,Zezhong Yu,Jiafa Hou,Yifeng Deng,Zhenlei Zhou,Zhiyong Zhao,Jun Cui
T-2 and HT-2 toxins belong to mycotoxins which are found in human foods and animal chow. We investigated the toxicity and oxidative stress induced by T-2/HT-2 in broilers and chicken hepatocytes. Maize cultures of Fusarium poae was fed to broilers for 42 d, and the physiological index, biochemical index and expression of mRNAs related to oxidative stress were analyzed. Chicken hepatocytes were treated with different levels of T-2/HT-2, and the following parameters were detected: cell viability, GSH and MDA concentration, LDH leakage , activities of ALT/AST, ROS , GSH-PX, SOD and CAT , and expression of mRNA related to oxidative stress. In vivo, high levels of mycotoxins (4 mg/kg T-2 and 0.667 mg/kg HT-2) in feed caused significant reductions in body weight, weight gain, and serum total protein, and significant increases in feed conversion ratio, ALP , ALT/AST activities, and expression of mRNA related to oxidative stress. In vitro, cells treated with T-2/HT-2 showed reductions of GSH concentration and significant increases in LDH leakage, ALT/AST ROS, GSH-PX, SOD and CAT activities, MDA concentration, and expression of mRNA related to oxidative stress. Consequently, F. poae culture material and T-2/HT-2 induced toxicity and oxidative stress in vivo and in vitro, respectively.
研究领域:
兽医学
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毒理学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2024
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IF 2.8
Yuqi Yin,Weiyang Sun,Xuan Wang,Jiayue Chen,Hongyan Zeng,Sifan Hao,Lin Ren,Li Yong,Chunying Luo,Xiaoli Zou
Background Poisonings caused by plant toxins and mycotoxins occur frequently, which do great harm to human health and social public health safety. When a poisoning incident occurs, biological samples are commonly be used to conduct the detection of toxic substances and their metabolites for targeted clinical treatment and incident analysis. Objective To establish an efficient and accurate analysis method of 39 phytotoxins and mycotoxins in blood and urine by high performance liquid chromatography quadrupole tandem orbitrap mass spectrometry (HPLC-Orbitrap MS). Method After 3 mL of methanol being added to 1 mL blood and urine respectively for extraction and protein precipitation, the supernatant was injected into HPLC-Orbitrap MS for analysis. The phytotoxins and mycotoxins were separated by Hypersil GOLD PFP column with gradient elution using methanol-5 mmol/L ammonium acetate as mobile phase. The data were collected in ESI positive ion mode using Full MS/dd-MS 2 for mass spectrometry detection. Result The mass database of 39 phytotoxins and mycotoxins was developed, and accurate qualitative analysis can be obtained by matching with the database using the proposed identification criteria. Limit of detections (LODs) were 1.34 × 10 −4 ∼ 1.92 ng/mL and 1.92 × 10 −4 ∼ 9.80 ng/mL for blood and urine samples, respectively. Limits of quantification (LOQ) of toxins in blood and urine ranged from 4.47 × 10 −4 ∼ 6.32 ng/mL and 6.39 × 10 −4 ∼ 32.67 ng/mL, respectively. Intra-day relative standard deviations (RSDs) were 0.79 % ∼ 10.90 %, and inter-day RSDs were 1.08 % ∼ 18.93 %. The recoveries can reach 90 % ∼ 110 % with matrix matching calibration curves. Conclusion The established method is simple and rapid to operate, which can complete the sample analysis within 30 min, providing technical support for clinical poisoning treatment and public health poisoning analysis.
研究领域:
毒理学
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分析化学
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临床医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
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2015
·
IF 2.806
Zhiyong Zhao,Na Liu,Lingchen Yang,Jianhua Wang,Suquan Song,Dongxia Nie,Xianli Yang,Jiafa Hou,Aibo Wu
The primary objective of this study was to synthesize three types of cross-linked chitosan polymers and further investigate their adsorption capability for multiple mycotoxins , including aflatoxin B 1 (AFB 1 ), ochratoxin A (OTA), zearalenone (ZEN), fumonisin B 1 (FB 1 ), deoxynivalenol (DON) and T-2 toxin (T2). Among these synthetic adsorbents, cross-linked chitosan-glutaraldehyde complex presented the highest adsorption capability for AFB 1 (73%), OTA (97%), ZEN (94%) and FB 1 (99%), but no obvious adsorption for DON and T2 (<30%). The effect of various incubation conditions (contact time, dosage and pH) was also studied. Subsequently, the experimental data were fitted to Langmuir, Freundlich and Hill models. The best fitting model to describe AFB 1 and FB 1 adsorption was Langmuir model ( R 2 ≥ 0.99), with the theoretical maximum adsorption amounts of 5.67 mg/g for AFB 1 and 15.7 mg/g for FB 1 . The Hill model was the best model for OTA and ZEN adsorption ( R 2 > 0.98), with the predicted maximum adsorption amounts were 24.8 mg/g for OTA and 9.18 mg/g for ZEN. In addition, the adsorption capability of adsorbent for the simultaneous presence of multiple mycotoxins was also evaluated in buffer system and simulated gastrointestinal condition. The results indicated that the coexisted multiple mycotoxins didn't affected the adsorption capability of adsorbent, whereas the adsorption amounts of toxins were decreased by some gastrointestinal components. The findings of this research suggest that chitosan–glutaraldehyde complex has the potential to be applied as multitoxin adsorbent material for reducing the combined adverse effect of multiple mycotoxins on humans and animals.
研究领域:
食品科学
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毒理学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2022
·
IF 6.556
Ming Li,Wenying Lu,Yuhao Mao,Xuchun Qiu,Daolin Du
It is desired and urgently needed to improve the sensitivity of immunochromatography assay (ICA) for hazardous chemicals. In this work, an enhanced ICA strip was established and evaluated for simultaneous, semi-quantitative and quantitative detection of ochratoxin A (OTA) and aflatoxin B 1 (AFB 1 ). The signal strategy based on gold growth on the surface of the E. coli K12 carrier was recommended, which was successfully self-assembled on the enhanced ICA with a double test line pattern. When used as a novel carrier, the E. coli K12 could provide a larger surface area, better biocompatibility and high loading capacity, which was of great help to improve the performance of the ICA. By the naked eye, the semi-quantitative limit of detection (semi-Q-LOD) reached 0.03 ng/mL for both OTA and AFB 1 (17-fold and 33-fold lower than the conventional ICA strip). By the digitized strip reader, the quantitative LODs (Q-LODs) were all identified as 0.01 ng/mL for two mycotoxins (10-fold improvement), with detection ranges of 0.01–0.5 ng/mL for OTA and 0.01–0.2 ng/mL for AFB 1 . Furthermore, the high reliability and applicability of the ICA were confirmed by its good correlation with liquid chromatography-tandem mass spectrometry (LC-MS/MS). The advantages of the improved sensitivity, high efficiency and cost savings had been reflected for the ICA. This study could provide an important reference method for the sensitive, simultaneous, rapid and on-site monitoring of multicomponent contaminants.
研究领域:
食品安全检测
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分析化学
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TOXICON
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2020
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IF 2.201
Lingchen Yang,Di Tu,Yingxin Wu,Wei Liu,Yi Hu,Tanbin Liu,Lei Tan,Yalan Li,Hongyu Lei,Yang Zhan,Naidong Wang,Zhibang Deng,Shiyin Guo,Aibing Wang
T-2 and HT-2 widely found in food products can seriously affect human and animal health. In this study, sterilized corn was inoculated with F. poae and incubated to allow fungal growth before being examined via liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) to determine the concentrations of T-2/HT-2. Broilers were then fed with a mix of moldy corn and normal feed at different ratios to obtain different toxin doses. After 35 days, the contaminated feed was replaced with mycotoxin-free feed and the distribution and concentration of residual toxins in the tissues and organs of the chickens were examined at different time points. The results showed that at the time of feed replacement (0 h), T-2 residue was present at significantly higher concentrations in the lungs and small intestines than in other tissues ( P < 0.05). In addition, T-2 concentrations increased in a dose-dependent manner in the tissues of chickens in the low-, medium-, and high-dose groups; however, the differences in concentration between the groups were not statistically significant. The HT-2 content (0 h) in the livers and small intestines was significantly higher than that in other tissues ( P < 0.05). At 48 h post-feed replacement, the concentration of T-2 dropped below detectable levels in all tissues while HT-2 could still be detected at 192 h post-feed replacement. Thus, this study reveals the distribution and persistence of residual T-2/HT-2 from moldy feed in broilers, providing a reference for the detection of these toxins in animal-derived food products and a theoretical basis for formulating food-safety and quality standards.
研究领域:
动物科学
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毒理学
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食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2019
·
IF 7.65
Zong-Yao Qian,Xiao Chen,He-Te Zhu,Jun-zhe Shi,Ting-Ting Gong,Qi-Ming Xian
In this study, the biosynthesis of microcystins (MCs) was investigated after long-term nitrogen-starved conditions in cyanobacterium Microcystis aeruginosa . The results demonstrated that the algal cells were able to survive in a non-growing state with nitrogen starvation for more than one month. The physiological properties of the algal cells were studied to elucidate the mechanisms of viability under nitrogen-deprivation conditions. After the state of nitrogen chlorosis, new toxins could be resynthesized and tracked using 15 N-stable isotope-labelled nitrogen. Nitrogen starvation of nutritionally replete cells resulted in a significant increase of microcystin-LY (MC-LY), thereby suggesting that MC-LY may undergo catabolism to provide nitrogen or that MC-LY may be produced to play an important role in the cell in response to nitrogen deprivation. The rank order of different types of nitrogen in algal cells assimilation was N-ammonium > N-urea > N-nitrate > N-alanine. The relationship between the production of toxin variants and various environmental conditions is an interesting issue for future research and may help improve the understanding of the ecological role of cyanobacterial toxins .
研究领域:
环境科学
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微生物学
✓ 已验证
DOI
PubMed
高影响因子
ENVIRONMENTAL RESEARCH
·
2025
·
IF 7.7
Huiting Yang,Yujia Yao,Wei Chen,Xiaohong Gu,Huihui Chen,Qingfei Zeng,Zhigang Mao,Tao Xiang
Cyanobacterial blooms can generate various toxic metabolites in freshwater, and pose serious threats to drinking water safety and human health. Although microcystins (MCs) have been detected in many freshwater ecosystems in China, little is known about the other cyanotoxins. An investigation of six eutrophic lakes (i.e. Hulun Lake, Wuliangsuhai Lake, Chaohu Lake, Taihu Lake, Xingyun Lake, and Dianchi Lake) in different geographical locations of China was performed during the summer of 2022 to determine the occurrence of various cyanotoxins (i.e. anatoxin-a (ATX), cylindrospermopsin (CYN), and MCs) in water column and their possible risks, and to evaluate the related environmental factors. MCs levels in sediment of these lakes were also investigated. MCs were the primary cyanotoxins in the water column of investigated lakes. The mean MCs contents in water column of Hulun Lake, Wuliangsuhai Lake, Chaohu Lake, Taihu Lake, Xingyun Lake, and Dianchi Lake were 3.61, 0.13, 3.60, 2.18, 0.57, and 2.56 μg/L, respectively. The total MCs levels in water column exceeded 1 μg/L in some areas of these lakes except Wuliangsuhai Lake. Replete nitrogen and/or phosphorus levels seemed to be related to MCs production. ATX can be detected in these lakes except Xingyun Lake at ng/L levels. CYN can be detected in all lakes at ng/L levels. However, the levels of ATX and CYN appear to be not significantly associated with environmental factors. MCs and CYN can pose a high or moderate risk for humans and aquatic organisms in some areas of these lakes, while ATX can pose a low or no risk for humans and aquatic organisms in most areas of these lakes. MCs can also be detected in sediment of all lakes at ng/g levels. This research emphasizes the necessity for long-term monitoring of different cyanotoxins in eutrophic lakes, and the implementation of nutrient control and management strategies.
研究领域:
生态学
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环境科学
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毒理学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2024
·
IF 4
Lijun Yin,Peipei Xu,Junjie Ren,Jing Shi,Runbing Huang,Yunzhen Liu,Yudong Lu,Ruiyun You
Stonehouse clam toxin (STX) is a prevalent neuroparalytic toxin found among shellfish toxins . When consumed by humans, it disrupts neuronal conduction , potentially leading to quadriplegia , headache , shock, and even respiratory failure-induced death. This study introduces a highly sensitive and specific method for detecting shellfish toxins . The approach involves utilizing graphene gold-loaded compounds alongside stone house clam toxin aptamers and their complementary chains. The layered structure of graphene creates numerous hot spots among nanoparticles , while its arched spatial configuration offers an enhanced specific surface area , resulting in improved Surface-Enhanced Raman Scattering (SERS). By combining the signaling molecule Bodipy R6G-modified Apt with GO-Au loading compounds, distinctive Raman characteristic peaks emerge. Due to the strong affinity between Apt and STX, the Apt carrying the signaling molecule migrates away from the SERS aptamer probe's surface, thereby attenuating the original SERS signal intensity and facilitating ultra-sensitive shellfish toxin detection. The detection limit for the toxin was determined to be 5.47 nM, with a recovery rate ranging from 97.60 % to 108.32 %, affirming the applicability of this design for quantitative and specific shellfish toxin detection, signifying its considerable practical significance.
研究领域:
毒理学
·
分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2015
·
IF 2.729
Qiutao Liu,Weijun Kong,Weiying Guo,Meihua Yang
An ultra fast liquid chromatography coupled with tandem mass spectrometry (UFLC-MS/MS) method was developed and validated for simultaneous analysis of multi-class mycotoxins including aflatoxins (AFB 1 , AFB 2 , AFG 1 and AFG 2 ), ochratoxin A (OTA), fumonisins (FB 1 and FB 2 ) and zearalanone (ZEN) in 20 batches of Angelica sinensis samples collected from different markets and stores in China. The eight mycotoxins were extracted and cleaned up by using QuEChERS-based procedure, and then were quantified under the multiple reaction monitoring (MRM) together with positive and negative ionization modes. Focusing on the optimization of extraction and clean-up conditions, as well as UFLC separation and MS/MS parameters of targeted analytes, the developed method expressed good linearity for the eight mycotoxins within their respective linear ranges with correlation coefficients all higher than 0.9974. The limits of detection (LODs) and quantification (LOQs) ranged from 0.005 to 0.125 μg/kg and from 0.0625 to 0.25 μg/kg, respectively. Recoveries for spiked A. sinensis sample at three different levels were all above 78.9% with relative standard deviations (RSDs) below 6.36% for all analytes. Analysis of real samples demonstrated that two visibly moldy A. sinensis samples were detected with AFB 1 of 2.07 and 2.92 μg/kg, and AFG 1 of 2.84 and 1.53 μg/kg. The proposed quantitative method with significant advantages including simple pretreatment, rapid determination and high sensitivity would be the preferred candidate for the determination and quantification of multi-class mycotoxin contaminants in complex matrixes, which well fulfilled the maximum residue limits (MRLs) from various countries.
研究领域:
食品科学
·
分析化学
·
中药学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Hongwen Xu,Jiadi Sun,Haiming Wang,Yinzhi Zhang,Xiulan Sun
A new, green, and cost-effective magnetic solid-phase extraction of aflatoxins and ochratoxins from edible vegetable oils samples was developed using polydopamine-coated magnetic multi-walled carbon nanotubes (PDA@Fe 3 O 4 -MWCNTs) as the absorbent. PDA@Fe 3 O 4 -MWCNTs nanomaterials were prepared by chemical co-precipitation and in situ oxidation and self-polymerization of dopamine and was characterized. Factors affecting MSPE and the adsorption behavior of the adsorbent to mycotoxins were studied, and the optimal extraction conditions of MSPE and the complexity of the adsorption process were determined. Based on this, the magnetic solid-phase extraction-high-performance liquid chromatography-fluorescence detection method (MSPE-HPLC-FLD) was established for determining six mycotoxins [aflatoxin B 1 (AFB 1 ), AFB 2 , AFG 1 , and AFG 2 , and ochratoxin A (OTA) and OTB)] in vegetable oils. The recovery was 70.15%~89.25%, and RSD was ≤6.4%. PDA@Fe 3 O 4 -MWCNTs showed a high affinity toward aflatoxins and ochratoxins, allowing selective extraction and quantification of aflatoxins and ochratoxins from complex sample matrices.
研究领域:
食品安全
·
分析化学
·
环境科学
✓ 已验证
DOI
高影响因子
FOOD CONTROL
·
2022
·
IF 6.652
Hui Li,Yan-yan Huang,Keren Agyekumwaa Addo,Yi-gang Yu,Xing-long Xiao
The inhibitory mechanism of CUM on Staphylococcus aureus exotoxins and invasive enzyme, and the effect of CUM on sauced beef were investigated. Treatment with sub-minimum inhibitory concentrations of CUM observed very little decrease in cell density after 24 h. Additionally, CUM reduced hemolytic activity by inhibiting the formation of heptamer, and inhibited the content of enterotoxin in culture medium and sauced beef. The transcription of genes encoding α-hemolysin, staphylococcal enterotoxin A and Panton-Valentine leukocidin were down-regulated after treatment with CUM. Compared with the control group, the addition of CUM caused a significant decrease in S. aureus counts, total volatile base nitrogen, acid value, and thiobarbituric acid reactive substances of sauced beef after 9 d. Furthermore, CUM improved the redness (a*) of sauced beef. These results confirmed that CUM could effectively restrain the synthesis of S. aureus toxin, and the addition of CUM to sauced beef was the potential way to inhibit the growth of food pathogens and maintain quality profiles.
研究领域:
食品科学
·
微生物学
·
毒理学
·
食品安全
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）