JOURNAL OF ANALYTICAL CHEMISTRY
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2023
·Vol. 78
·期 2
·213-221
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IF 1.1
Suo Lili,Hu Meihua
Abstract A new specific sorbent entitled molecularly imprinted polymer (Fe 3 O 4 @PDA MIP) was fabricated on the surface of ferroferric oxide nanoparticles with the dummy template of 5,7-dimethoxycoumarin through self-assembly of dopamine which is efficient for recognition and determination of aflatoxin B in food samples coupled with UPLC-FLD with a large volume flow cell. The prepared MIPs were characterized, and the adsorption properties were evaluated. The maximum adsorption capacities were 0.46 mg/g for aflatoxin B1 and 0.047 mg/g for aflatoxin B2. Under optimized conditions, the calibration plots are linear in the range of 0.005–0.5 and 0.001–0.1 ng/mL with LODs of 0.0024 and 0.0004 ng/mL for aflatoxin B1 and aflatoxin B2, respectively. The recoveries from spiked samples are 89.0–105.0% with RSDs of 5.6–7.7% in case of corn, peanut and edible oil. And after 7 cycles, the adsorption capacity of the MIPs did not decrease significantly.
研究领域:
分析化学
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食品安全
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
Acta Biomaterialia
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2019
·
IF 6.638
Yueqi Zhao,Suraj Maharjan,Yuanqing Sun,Zhe Yang,Enfeng Yang,Nan Zhou,Laijin Lu,Andrew K. Whittaker,Bai Yang,Quan Lin
A retrograde transportation nerve probe, Au nanodots-cholera toxin B subunit (AuNDs-CTB), are prepared and fully characterized, which emit bright red fluorescence and show high quantum yield (7.2%) and good stability. The fluorescence emitted by the AuNDs is constant across a wide pH range (4–10) and after prolonged UV irradiation (>4 h). Previously, CTB has shown targeting characteristic for nerve cells with high sensitivity and effectiveness. After linking CTB to AuNDs through amidation reactions, AuNDs-CTB are obtained with excellent fluorescence property, nerve target characteristic, and, particularly, neural retrograde transportation feature. The red emission of the AuNDs-CTB is well distinguished from the blue autofluorescence of normal tissues, which provides potential for detection by naked eyes. Further, the fluorescence emission intensity maintains for 10 days in vivo, suggesting great utility for long-time monitoring and sensing of the nerve tissue. Furthermore, the AuNDs-CTB with bright red fluorescence can travel through the peripheral nerve to the spinal cord rapidly by retrograde transportation. The transportation occurs for a long distance (>5 cm) within only 2 days after injection of the AuNDs-CTB into the sciatic nerve. The present study exhibits a novel method for nerve visualization and drug delivery. Statement of significance Au nanodots (AuNDs) conjugated with cholera toxin subunit B (CTB) have been developed for nerve labeling and neural retro-transporting. The red fluorescence from AuNDs-CTB is stable in vitro (pH 4–10 and 4 h UV irradiation) and in vivo (for a long time, more than 10 days). When injecting AuNDs-CTB into the sciatic nerve located at the midpiece of the thigh, the targeted nerve emits bright red fluorescence under UV light. Furthermore, the nerve can retrograde transport the AuNDs-CTB to the spinal cord for a distance of more than 5 cm just in 2 days. This work exhibits a novel method for nerve visualization by naked eyes and demonstrates the potential for intraoperative navigation.
研究领域:
神经生物学
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生物医学工程
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Chemosensors
·
2023
·Vol. 11
·期 8
·453
·
IF 4.2
Kai Mao,Lili Tian,Yujie Luo,Qian Li,Xi Chen,Lei Zhan,Yuanfang Li,Chengzhi Huang,Shujun Zhen
Enzyme-linked immunosorbent assay (ELISA) is one of the most commonly used method for the detection of staphylococcal enterotoxin B (SEB), the main protein toxin causing staphylococcal food poisoning. However, the traditional ELISA reaction needs to be stopped by sulfuric acid to obtain stable colorimetric signal, and it is easily influenced by a colored sample. In order to address this problem, a new ELISA method using zeolite imidazolate skeleton-8 metal-organic framework (ZIF-8 MOF) as a light scattering (LS) reporter for SEB detection was developed in this work. ZIF-8 MOF has the characteristics of high porosity, large specific surface area, clear pore structure, and adjustable size, which is one of the most representative MOFs constructed from Zn2+and 2-methylimidazole (2-mIM). The 2-mIM ligand of ZIF-8 exhibited antioxidant activity and can strongly react with H2O2, which could destroy the structure of ZIF-8, resulting in the obvious decrease in LS intensity. We combined this specific reaction with the sandwich immune reaction to construct the LS ELISA method for the successful detection of SEB. This method is more reliable than commercial tests kits for the detection of colored samples, and it is simple, sensitive, and selective, and has great potential in the detection of other toxins by simply changing the corresponding recognition units.Keywords:ELISA;light scattering;metal organic framework;ZIF-8;staphylococcal enterotoxin B
研究领域:
生物化学
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食品安全检测
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免疫学
✓ 已验证
产品关联
DOI
高影响因子
Pharmaceutics
·
2023
·Vol. 15
·期 5
·1493
·
IF 5.4
Chaojun Song,Yutao Liu,Jinwei Hu,Yupu Zhu,Zhengjun Ma,Jiayue Xi,Minxuan Cui,Leiqi Ren,Li Fan
Highly sensitive staphylococcal enterotoxin B (SEB) assay is of great importance for the prevention of toxic diseases caused by SEB. In this study, we present a gold nanoparticle (AuNP)-linked immunosorbent assay (ALISA) for detecting SEB in a sandwich format using a pair of SEB specific monoclonal antibodies (mAbs) performed in microplates. First, the detection mAb was labeled with AuNPs of different particle sizes (15, 40 and 60 nm). Then the sandwich immunosorbent assay for SEB detection was performed routinely in a microplate except for using AuNPs-labeled detection mAb. Next, the AuNPs adsorbed on the microplate were dissolved with aqua regia and the content of gold atoms was determined by graphite furnace atomic absorption spectrometry (GFAAS). Finally, a standard curve was drawn of the gold atomic content against the corresponding SEB concentration. The detection time of ALISA was about 2.5 h. AuNPs at 60 nm showed the highest sensitivity with an actual measured limit of detection (LOD) of 0.125 pg/mL and a dynamic range of 0.125–32 pg/mL. AuNPs at 40 nm had an actual measured LOD of 0.5 pg/mL and a dynamic range of 0.5 to 128 pg/mL. AuNPs at 15 nm had an actual measured LOD of 5 pg/mL, with a dynamic range of 5–1280 pg/mL. With detection mAb labeled with AuNPs at 60 nm, ALISA’s intra- and interassay coefficient variations (CV) at three concentrations (2, 8, and 20 pg/mL) were all lower than 12% and the average recovery level was ranged from 92.7% to 95.0%, indicating a high precision and accuracy of the ALISA method. Moreover, the ALISA method could be successfully applied to the detection of various food, environmental, and biological samples. Therefore, the successful establishment of the ALISA method for SEB detection might provide a powerful tool for food hygiene supervision, environmental management, and anti-terrorism procedures and this method might achieve detection and high-throughput analysis automatically in the near future, even though GFAAS testing remains costly at present.Keywords:gold nanoparticles;gold nanoparticle-linked immunosorbent assay;staphylococcal enterotoxin B
研究领域:
生物化学
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医学检测
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食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Yijie Wang,Yue Huang,Yan Zhang,Henan Wang
Staphylococcal enterotoxins as common bacterial toxins can bring about food contamination and lead to severe foodborne illnesses, so facile and sensitive detection of staphylococcal enterotoxins is significant to ensure food safety and protect public health. In this work, an ultrasensitive biosensor for staphylococcal enterotoxin B (SEB) detection is developed based on the novel electrochemical nanoprobe named MXene/AgNPs@pSC4/MB, which is prepared by functionalizing MXene with para-sulfonatocalix[4]arene-decorated silver nanoparticles (AgNPs@pSC4) and electroactive signal molecules methylene blue (MB). The collaborative effect of AgNPs and MXene endows the fabricated electrochemical nanoprobe with excellent conductivity and admirable loading capacity of signal molecules to significantly enhance the response of biosensor, while pSC4 as the robust linker can bridge the nanoprobe and target SEB by binding with multifarious amino acid residues on SEB. With the specific aptamer immobilized on the electrode as the identifying element to capture SEB, the electrochemical nanoprobes MXene/AgNPs@pSC4/MB are anchored to SEB via pSC4, producing remarkably amplified signal output. The proposed electrochemical biosensor facilitates the ultrasensitive detection of SEB with a low detection limit of 1.76 × 10 −7 ng/mL in addition to the merits of facile operation and rapid response. Moreover, benefiting from the high chemical stability and good resistance to fouling and passivation of the electrochemical nanoprobe, the biosensor exhibits long-lasting stability, good accuracy and anti-interference ability in SEB detection. The reliable detectability of the biosensor has also been examined in the analysis of real food samples, demonstrating potential application toward food safety monitoring.
研究领域:
食品安全检测
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生物传感
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纳米技术
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微生物学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Microbiology
·
2025
·
IF 4.5
Yulei Tai,Meng Zhang,Yuning Han,Hui Hu,Shan Lin,Fangya Zhai,Menglun Tian,Xiaojun Song,Shuangshuang Wan,Yu Chen,Dazhi Jin
Introduction: Niclosamide (NIC) has significant potential as a clinical therapeutic agent for Clostridioides difficile infection (CDI); however, its strong hydrophobicity hampers its oral bioavailability, and its active effects against C. difficile remain unclear.Methods: Niclosamide-loaded controlled-release hyaluronic acid-modified poly (lactic-co-glycolic acid) naosphernes (NIC@PLGA-HAs) were synthesized using an oil-in-water emulsion technique and their effects on C. difficile cell growth, spore germination, biofilm formation, and NIC interaction sites with C. difficile toxin B (TcdB) were analyzed.Results: NIC@PLGA-HAs exhibited enhanced solubility and stability, with a water contact angle on a hydrophilic surface of 65.1° and a zeta potential of 31.57 ± 2.08 mV, and pH-responsive (pH 7.4) controlled-release characteristics compared to free NIC. The NIC@PLGA-HAs killed C. difficile vegetative cells at a minimum inhibitory concentration (MIC) of 4 μg/mL. When C. difficile cells were treated with NIC@PLGA-HAs at the 1/4 MIC, spore germination and biofilm formation were significantly inhibited compared to those in untreated cells (P < 0.01). NIC was found to interact with the receptor-binding domain of TcdB at 24 amino acid sites via an enthalpy-driven reaction (enthalpy change, 36.21 kJ/mol and entropy change, 212.9 J⋅mol/K). In vivo experimental findings in Mongolian gerbils indicated that NIC@PLGA-HAs outperformed free NIC in reducing pathological damage, diarrhea severity, weight loss, and TcdB production and enhanced the survival rate.Conclusion: These findings presented the therapeutic potential of NIC@PLGA-HAs with high solubility and stability, which simultaneously exerted multiple biological activities against C. difficile.
研究领域:
药剂学
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微生物学
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纳米技术
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药物递送
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传染病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Huiping Feng,Yue Sun,Jiang Wang,Jiwei Xu,Xihui Mu,Bin Du,Zhaoyang Tong,Bing Liu
Staphylococcal enterotoxin B (SEB) is a major pathogenic factor in foodborne diseases, and its rapid and sensitive detection is crucial for early warning of food safety risks and public health protection. In this study, a colorimetric immunosensing system for SEB detection was constructed based on a magnetic covalent organic framework (COF) composite loaded with platinum nanoparticles (Pt NPs) that exhibit dual enzymatic activities. The effective loading of Pt NPs endows the composite with excellent peroxidase (POD) and oxidase (OXD)-like activities. The COF matrix not only provides abundant binding sites for Pt NP dispersion but also enhances substrate adsorption, thereby significantly improving catalytic efficiency. By introducing an Fe3O4 core, the nanocomposite enables facile preparation of antibody–nanoparticle conjugates through separation and enrichment functions. The Fe3O4@COF@Pt nanocomposite demonstrates excellent peroxidase- and oxidase-like activities, capable of decomposing H2O2 and O2 to generate hydroxyl radicals (˙OH) and superoxide anions (O2˙−), which catalyze the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into blue oxidized TMB (OXTMB), resulting in a visible color change for signal output. The sensor achieves specific detection by utilizing its peroxidase-mimicking activity, exhibiting a linear detection range of 1–200 ng mL−1 with a detection limit as low as 0.395 ng mL−1. This nanozyme-linked immunosorbent assay (NLISA) based on Fe3O4@COF@Pt offers a highly sensitive and convenient platform for SEB detection.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Beibei Lu,Jialei Bai,Jingyang Zhang,Huijing Shen,Minglin Wang,Yujing Lian,Zhixian Gao,Yuan Peng
Colorimetric sensing materials have a rising demand in detection applications, especially in the field of naked-eye detection. Herein, a novel iridescent polymeric film that can respond to different Staphylococcus aureus enterotoxin B (SEB) concentrations in the visible range is reported. This film is fabricated by polymerization of methacrylic acid (MAA) and ethylene dimethacrylate (EGDMA) owing an inverse opal array (IOA) structure, and gold nanoparticles (AuNPs) are enchased on the polymer skeleton. This composite structure facilitates the film to respond to SEB at a LOD of 4.29 fg mL −1 , making it the best performing colorimetric material to date. Cryo-Scanning Electron Microscopy and 3D AFM were used to observe the morphology change of the iridescent polymeric film after SEB binding. This film possesses great advantages for ultrasensitive visual detection of real samples. In addition, the iridescent polymeric film has the potential to become a universal sensing material, realizing the ultrasensitive visual detection of hazardous substances without equipment in the field by replacing the recognition element.
研究领域:
材料科学
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分析化学
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生物传感
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2018
·
IF 4.244
Fang Tan,Xiaoxue Xie,Aiqing Xu,Keqin Deng,Yunlong Zeng,Xiumei Yang,Haowen Huang
Fluorescent eggshell membrane-templated gold nanoclusters (Au-ESM) can be obtained in a facile and low-cost manner in this study. The fluorescence of the Au-ESM may be significantly quenched by mercapto-compounds and peroxidase-like activity of Au-ESM could be regulated by the reaction process with glutathione . Moreover, the catalytic activity of the mimetic enzyme membrane could be modulated by immunoreactions. An immunoassay for colorimetric determination of staphylococcal enterotoxins B (SE-B) using colored gold nanoparticles was established based on the catalytic activity adjusted by the target molecules. This colorimetric assay can detect SE-B at the concentration range of 0.4–20 ng/mL and the limit of detection (LOD) is 0.12 ng/mL. As a practical application, the proposed colorimetric assay was further utilized to detect SE-B in food samples such as flour, corn and rice, requiring very low volume of sample and exhibiting great sensitivity and high accuracy, which provides promising platform for development of point-of-care diagnostic devices with biomedical and food safety applications.
研究领域:
纳米技术
·
分析化学
·
食品安全
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2013
·
IF 4.387
Shijia Wu,Nuo Duan,Xiaoyuan Ma,Yu Xia,Hongxin Wang,Zhouping Wang
An ultrasensitive fluorescence resonance energy transfer (FRET) bioassay was developed to detect staphylococcal enterotoxin B (SEB), a low molecular exotoxin, using an aptamer-affinity method coupled with upconversion nanoparticles (UCNPs)-sensing, and the fluorescence intensity was prominently enhanced using an exonuclease-catalyzed target recycling strategy. To construct this aptasensor, both fluorescence donor probes (complementary DNA 1 –UCNPs) and fluorescence quencher probes (complementary DNA 2 –Black Hole Quencher 3 (BHQ 3 )) were hybridized to an SEB aptamer, and double-strand oligonucleotides were fabricated, which quenched the fluorescence of the UCNPs via FRET. The formation of an aptamer–SEB complex in the presence of the SEB analyte resulted in not only the dissociation of aptamer from the double-strand DNA but also both the disruption of the FRET system and the restoration of the UCNPs fluorescence. In addition, the SEB was liberated from the aptamer–SEB complex using exonuclease I, an exonuclease specific to single-stranded DNA, for analyte recycling by selectively digesting a particular DNA (SEB aptamer). Based on this exonuclease-catalyzed target recycling strategy, an amplified fluorescence intensity could be produced using different SEB concentrations. Using optimized experimental conditions produced an ultrasensitive aptasensor for the detection of SEB, with a wide linear range of 0.001–1 ng mL −1 and a lower detection limit (LOD) of 0.3 pg mL −1 SEB (at 3 σ ). The fabricated aptasensor was used to measure SEB in a real milk samples and validated using the ELISA method. Furthermore, a novel aptasensor FRET assay was established for the first time using 30 mol% Mn 2+ ions doped NaYF 4 :Yb/Er (20/2 mol%) UCNPs as the donor probes, which suggests that UCNPs are superior fluorescence labeling materials for food safety analysis.
研究领域:
食品安全分析
·
生物化学
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纳米技术
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Xueyu Chang,Yaqian Cheng,Xinke Wang,Yonghui Wang,Xueli Liu,Tie Han,Zhixian Gao,Huanying Zhou
A fluorescent biosensor strategy was developed in combination with immunomagnetic separation for rapid and sensitive detection of staphylococcal enterotoxin B (SEB). Magnetic nanoparticles (MNPs) modified with aptamer of SEB could capture the SEB. Then the gold nanoparticles (AuNPs) fluorescent probe was added and a “sandwich structure” was formed between AuNPs, SEB and MNPs. The MNPs-SEB-AuNPs structure could be separated with an additional magnetic field, which resulted the lower signals of AuNPs fluorescent probe. In optimal conditions, the current method displayed a broad quantitative range from 100 to 10 7 fg/mL and the limit of detection was 3.43 fg/mL. The recovery of SEB-spiked milk samples ranged from 92.00 to 119.00 %, which revealed that the developed method had great accuracy. Furthermore, the method was fast and economical for ultrasensitive detection. Therefore, the fluorescent biosensor based on MNPs-AuNPs is promising for the detection of other environmental and food pollutants.
研究领域:
生物技术
·
食品科学
·
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Xiaoxue Xie,Fang Tan,Aiqing Xu,Keqin Deng,Yulong Zeng,Haowen Huang
In this study, gold nanoclusters (AuNCs) in the presence of chitosan were prepared assisted by UV irradiation, which exhibit peroxidase-like property tested with H 2 O 2 /3,3,5,5-tetramethylbenzidine (TMB). The chitosan-AuNCs composite membrane also behaves peroxidase-like property and was characterized by TEM, AFM and SEM, which reveal the formed membrane was homogeneous and the AuNCs are predominantly spherical and well-dispersed without agglomeration in the membrane. A colorimetric assay was established by integrating immunoreactions occurred on the enzyme mimicking AuNC-chitosan composite membrane with varying colored GNPs serving as output signal. Three kinds of bacteria were chosen to serve as model bacteria to evaluate the selectivity of this sensor and Staphylococcus aureus ( S. aureus ), can be easily differentiated from Escherichia coli ( E. coli ) and Bacillus subtilis ( B. subtilis ). Such a colorimetric sensing is an extendable strategy due to the recognition element of probe may be replaceable on the catalytic AuNC-chitosan composite membrane. Staphylococcal enterotoxin B (SE-B) as low as 1.0 × 10 −12 g/mL may be sensitively detected by naked-eye readout. As a practical application, the developed colorimetric strategy was used for rapid onsite screening strategy for S. aureus and detecting SE-B in food samples. This colorimetric strategy provides promising platform for rapid onsite screening pathogenic bacteria and detection of enterotoxin in food safety and environmental monitoring.
研究领域:
纳米技术
·
分析化学
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食品科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）