NEW JOURNAL OF CHEMISTRY
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2025
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IF 2.5
Hao Guo,Dengke Zhuang,Zhiguo Yu,Liping Peng,Yanyan Ma,Zeyun Yang,Yu Yan,Yingfei Hui,Wu Yang
Bacillus anthracis is a highly dangerous zoonotic pathogen that poses a considerable threat to public health. Rapid and accurate detection of the biomarker 2,6-pyridinedicarboxylic acid (DPA) is necessary for the effective prevention and treatment of infections caused by this microorganism. Here, a new method for detecting DPA using a bi-emission ratio fluorescence sensor based on a bismuth organic skeleton (Bi-MOF) is developed. The dual-emission ratio fluorescent sensor (Eu@Bi-MOF) is prepared by Eu3+ functionalizing Bi-MOF, synthesized through using bismuth nitrate as the metal node and 1,3,5-phenyltricarboxylic acid as the ligand, with a post-synthetic modification method and exhibits a sensitive fluorescent response to DPA. In the presence of DPA, the fluorescence intensity of the Eu@Bi-MOF sensor at 438 nm remains unchanged, while the red fluorescence emission at 615 nm was significantly quenched due to the internal filtration effect (IFE). The fluorescence intensity ratio I438/I615 shows a good linear relationship with DPA concentration in the range of 0.5–60 μM, and the detection limit is as low as 0.36 μM. This method provides a sensitive and reliable alternative strategy for rapid DPA monitoring.
研究领域:
分析化学
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生物传感器
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材料科学
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病原体检测
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荧光光谱学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FLUORESCENCE
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2025
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IF 3.1
Yao Jie,Chen Hongfang,Yang Xiaohua,Iqbal Muzaffar,Bian Wei
Mercury ions (Hg 2+ ) are categorized as environmental pollutants, which distributed in water, soil, and food systems due to environmental contamination. Hence, designing a sensitive assay for the convenient determination of Hg 2+ is of great importance. Herein, S and O-doped graphite phase nitrogenized carbon quantum dots (S, O-C 3 N 4 QDs) was encapsulated within a europium -based metal-organic framework (Eu-MOF) to construct a novel ratiometric fluorescent nanoprobe for the quantitative detection of Hg 2+ . The native emission of S, O-C 3 N 4 QDs at 445 nm is used as a response signal, while Eu-MOF with fluorescence offers a reference signal at 619 nm. Hg 2+ exhibits high affinity for the surface functional groups of S/O-C 3 N 4 QDs, forming non-fluorescent chelation complexes that induce static quenching. This results in significant attenuation of the fluorescence intensity at 445 nm, while the emission at 619 nm remains invariant. A ratiometric fluorescence sensing platform was established based on the intensity ratio (F 445 /F 619 ) for the selective detection of Hg 2+ . The linear range of S, O-C 3 N 4 QDs/Eu-MOF of Hg 2+ was 0.25–35 µM and with a detection limit of 4.3 nM. The satisfying results demonstrate the effectiveness of the developed S, O-C 3 N 4 QDs/Eu-MOF-based fluorescence probe for Hg 2+ detection, highlighting its promising potential for environmental monitoring applications.
研究领域:
环境科学
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分析化学
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材料科学
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2025
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IF 4.6
Yaqiong Zhang,Junping Lv,Qin Zhou,Kaile Chu,Min Li,Yayuan Li,Jie An,Zhifang Wu,Sijin Li
Ratiometric fluorescent (RF) sensors, leveraging their inherent calibration mechanism, can greatly reduce the interference of irrelevant factors and exhibits superior detection sensitivity and more attractive visual detection effects in the process of quantitative analysis. Up to now, there are few probes that can detect fluorine by the proportional fluorescence signal. Herein, we constructed a RF probe for determination of fluorine by confining self-synthesized red emitting organic small molecules 10-(diethylamino)-3-hydroxy-5,6-dihydrobenzo[ c ]xanthen-12-ium (HTD) into the microchannels and cavity structures of UiO-66-NH 2 , exhibiting two emission maxima at 428 nm and 616 nm, respectively. After introducing fluoride into the system of probe, a drastic fluorescence enhancement at 428 nm was emerged, whereas the emission peak at 616 nm underwent minor fluctuations. The probe that has been developed enables the sensitive and selective detection of fluoride ions, exhibiting excellent linear relationships in the range of 0–100 μM. The detection limit value was estimated to be 6.93 μM. This unique ratiometric luminescence behavior of probe toward fluoride resulted from the creation of hydrogen bonds between fluoride and amino groups in HTD@UiO-66-NH 2 . Notably, the probe could utilize as an effective tool for detecting fluoride in cells with high-quality results, which could have important implications for research in biology, toxicology, and related fields. Simultaneously, its corresponding portable online test strips can be easily acquired and employed for fluoride detection through visual colorimetric analysis. The proposed method offers an alternative technology for quantifying fluoride levels in real-life water samples.
研究领域:
分析化学
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生物传感
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环境监测
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分子探针
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毒理学
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转化医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FLUORESCENCE
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2025
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IF 3.1
Zhao Tengzhi,Wang Zhaofeng,Zhang Runke,Xu Hui,Wang Hua,Yu Ying
The antibiotic contamination in aquatic environments, particularly in aquaculture systems, poses substantial risks to ecological balance and human health. To address this issue, we engineered a novel ratiometric fluorescent probe utilizing dual-emission carbon dots (D-CDs) synthesized from sustainable biomass carrot and nitrogen-rich precursors (melamine and o-phenylenediamine) through an efficient one-pot hydrothermal approach. The D-CDs exhibited dual emission peaks at 425nm and 540 nm under 370nm excitation. The fluorescence intensity ratio (F 540 /F 425 ) demonstrated linear response toward tetracycline (TC) and oxytetracycline (OTC), with wider linear detection ranges of 0.027–133.3 µM for TC and 0.027–233.3 µM for OTC, respectively. Low detection limits (17.7 nM for TC and 22.04 nM for OTC) were also achieved. Mechanistic studies revealed that quenching resulted from static quenching and inner filter effects. The probe showed exceptional stability under high ionic strength and long storage conditions. Real-sample monitoring in lake water and aquaculture samples yielded recovery rates of 90.4-103.5% with RSD < 6.88%, which was validated by high-performance liquid chromatography (HPLC), demonstrating the promising potential of this probe. Graphical
研究领域:
环境科学
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分析化学
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纳米技术
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生物工程
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水污染控制
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2025
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IF 7.7
Yu Zhao,Huangmei Zhou,Longliang Qiao,Jinming Xu,Rodolphe Antoine,Lunhua Deng,Sanjun Zhang,Haifeng Ye
Precise measurements of tumor pH is essential for early cancer diagnose, treatment planning, and the development of anti-cancer drugs. Herein, we present a pH-sensitive, ratiometric fluorescent biosensor with tumor-targeting capabilities for real-time in vivo tumor pH detection. The biosensor, composed of glutathione-protected gold nanoclusters (AuNCs) conjugated with fluorescein-isothiocyanate (FITC), provides a reliable ratiometric fluorescence signal response to pH. The cyclic arginine-glycine-aspartic acid (c(RGDyC)) peptide was integrated into the sensor (denoted as c(RGDyC)-FITC@AuNCs) to confer tumor-targeting specificity. Upon excitation at 488 nm, c(RGDyC)-FITC@AuNCs exhibited dual-channel emissions at 515 nm and 710 nm, the ratio of which showed a 17-fold change across a pH range of 5.5 to 8.0. In addition, c(RGDyC)-FITC@AuNCs demonstrated excellent biocompatibility, long-term photostability, and low toxicity, enabling the quantitative monitoring of the pH changes during tumor cell apoptosis in real-time. Moreover, using tumor-bearing mouse models with induced acid-alkaline variations, c(RGDyC)-FITC@AuNCs successfully visualized and dynamically tracked in situ tumor pH. This biosensor offers a sensitive and accurate tool for tumor diagnosis, with significant potential in biomedical research and clinical studies.
研究领域:
生物医学工程
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纳米技术
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生物化学
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癌症研究
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分子成像
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荧光传感
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药学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）