ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2024
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IF 3.8
Wang Yu-Ling,Liu Xiang-Mei,Ren Shu-Wei,Cao Jun-Tao,Liu Yan-Ming
A highly efficient ratiometric electrochemiluminescence (ECL) immunoassay was explored by bidirectionally regulating the ECL intensity of two luminophors. The immunoassay was conducted in a split-type mode consisting of an ECL detection procedure and a sandwich immunoreaction. The ECL detection was executed using a dual-disk glassy carbon electrode modified with two potential-resolved luminophors (g-C 3 N 4 -Ag and Ru-MOF-Ag nanocomposites), and the sandwich immunoreaction using glucose oxidase (GOx)-modified SiO 2 nanospheres as labels was carried out in a 96-well plate. The Ag nanoparticles (NPs) acted as bifunctional units both for triggering the resonance energy transfer (RET) with g-C 3 N 4 and for accelerating the electron transfer rate of the Ru-MOF-Ag ECL reaction. When the H 2 O 2 catalyzed by GOx in the 96-well plate was transferred to the dual-disk glass carbon electrode, the doped Ag NPs in the two luminophors could be etched, thus destroying the RET between C 3 N 4 and the accelerated reaction to Ru-MOF, resulting in an opposite trend in the ECL signal outputted from the dual disks. Using the ratio of the two signals for quantification, the constructed immunosensor for a model target, i.e. myoglobin, exhibited a low detection limit of 4.7 × 10 –14 g/mL. The ingenious combination of ECL ratiometry, bifunctional Ag NPs, and a split-type strategy effectively reduces environmental and human errors, offering a more precise and sensitive analysis for complex samples. Graphical Abstract
研究领域:
分析化学
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生物医学工程
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DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2021
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IF 4.464
Jun Wan,You Shen,Liqiang Xu,Ruixue Xu,Jinfan Zhang,Haodi Sun,Chongyu Zhang,Chuanxia Yin,Xinxing Wang
The development of selective electrochemical response materials and new detection strategies are the research focus in the field of heavy metal ion (HMI) electroanalysis. Herein, a Ni(II)-based metal-organic framework (NH 2 -Ni-MOF) was synthesized and functionalized by electrochemically active molecule of ferrocene (Fc) via post-synthesis modification. Based on the Fc-functionalized NH 2 -Ni-MOF (Fc-NH 2 -Ni-MOF), a novel electrochemical ratiometric sensing platform was developed and applied for the simultaneous determination of various HMIs. The prepared NH 2 -Ni-MOF exhibits nanoplate structure, which is conducive to increase accessible electrode area and expose more active sites, thus promoting the adsorption and pre-concentration of HMIs. The modification of Fc on MOF not only enhances the electrical conductivity of the MOF material, but also provides an internal reference signal for the ratiometric analysis. Due to the excellent characteristics of the Fc-NH 2 -Ni-MOF, the as-prepared ratiometric electrochemical sensing platform exhibits wide linear ranges (0.001 μM to 2.0 μM for lead ions (Pb 2+ ), and 0.01 μM to 2.0 μM for copper ions (Cu 2+ ) as well as cadmium ions (Cd 2+ )) and high sensitivity (the detection limit toward Cu 2+ , Pb 2+ and Cd 2+ is 6.3 nM, 0.2 nM and 7.1 nM, respectively). In addition, compared with the non-ratiometric strategy, the reproducibility of ratiometric analysis is significantly improved. The developed electrochemical method with high sensitivity, selectivity and reliability exhibits bright application prospects in the detection of real samples. Moreover, the electrochemical application of MOF materials has been effectively expanded.
研究领域:
电化学
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分析化学
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材料科学
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环境科学
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2021
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IF 10.618
Xue-yun Yang,Yin-zhu Wang,Ling-ling Wang,Jia wan Zhu,Jie Zhao,Hui-long Zong,Chuan-xiang Chen
In this article, we developed a novel ECL ratiometry on a closed bipolar electrode (BPE) for the sensitively and accurately detection of miRNA-21. High quantum yield and low toxicity BNQDs was synthesized and coated at BPE cathode as an ECL emitter, while the anode of BPE was calibrated via another ECL material, Ir(df-ppy) 2 (pic) (Firpic). The electron neutrality at both ends of the BPE electrically coupled the reactions on each pole of the BPE. Therefore, one electrochemical sensing reaction could be quantified at one end of the BPE. By the hybridization of target miRNA-21 and hairpin, the glucose blocked in MSNs by the hairpin was released and reacted with glucose oxidase (GOD) to generate H 2 O 2 , thereby reducing the ECL signal of the cathode BNQDs/K 2 S 2 O 8 system and promoting ECL signal of anode Firpic/TPrA. Further, the G-quadruplex formed by unreacted hairpin bases consumed H 2 O 2 , which not only recovered the ECL of BNQDs, but also further improved the ECL emission of Firpic. Therefore, the concentration of miRNA-21 could be measured by the ECL ratio of BNQDs and Firpic. The data showed that the detection limit was 10 −15 M (S/N = 3) with the linear range of 10 −15 M to 10 −9 M. The strategy of the BPE-ECL ratio method based on BNQDs showed a good prospect in clinical application.
研究领域:
分析化学
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生物化学
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纳米技术
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分子生物学
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临床诊断
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PubMed
高影响因子
Journal of Materials Chemistry B
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2019
·Vol. 7
·期 11
·1926-1932
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IF 5.047
Fanyu Meng,Hua Chai,Xiaoyi Ma,Yuguo Tang,Peng Miao
Telomerase catalyzes the elongation of telomeres, which is closely associated with tumorigenesis. Therefore, development of reliable and convenient methods for telomerase activity analysis is critical for clinical diagnosis of cancers. However, most of the current probes for intracellular telomerase activity assay suffer from inevitable false-positive interferences. In this study, we have proposed a novel fluorescence resonance energy transfer (FRET)-based strategy for ultrasensitive monitoring intracellular telomerase activity with an amplification-free procedure. DNA nanoprobes constructed using a DNA tetrahedron and Flare DNA are designed to circumvent the problem of false-positive interference. Generally, telomerase catalyzes DNA extension and changes the configuration of the DNA nanoprobe, which finally leads to the increase of the distance between two labelled fluorophores. Based on the notably decreased FRET efficiency, a simple ratiometric sensor is established for the detection of telomerase activity at the single-cell level, which is much suitable for clinical applications. In addition, this method can be further applied in the detection of telomerase inhibitors, which is important for anti-cancer drug discovery.
研究领域:
分子生物学
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生物技术
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癌症研究
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纳米技术
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生物化学
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PubMed
高影响因子
危险品化学品经营许可证（带存储）