SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2024
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IF 4.3
Zetong Meng,Jing Liu,Ting Yu,Zhuobin Shang,Yu Wang,Shaomin Shuang
A novel ratiometric fluorescent probe, namely 5-[(3-dicyanoylidene −5.5-dimethyl) cyclohexenyl-1-ethenyl] salicylaldehyde-3′-hydroxybenzohydrazone (DCSH) is presented for the selective sensing of Zn 2+ ion in acetonitrile/water (2/3, pH 7.4) solution. Introducing Zn 2+ ions notably caused the peak emission of DCSH to shift from 560 nm to 646 nm, accompanied with a significant enhancement of its intensity. A vivid change in fluorescence color from yellow to red facilitated the immediate identification of Zn 2+ ions by visual observation. DCSH exhibits substantial Stokes shifts (110 and 196 nm), rapid detection capability (within 10 s) and high sensitivity to Zn 2+ ions, achieving a limit of detection of 31.2 nM. The response mechanism is supposed to involve the block of C = N bond isomerization and excited state intramolecular proton transfer (ESIPT) along with the enhancement of fluorescence through chelation (CHEF) effect. DCSH was effectively utilized for ratiometric fluorescence imaging to monitor exogenous Zn 2+ concentrations in HeLa cells. Significantly, DCSH is capable of monitoring elevated levels of Zn 2+ ion during apoptosis induced by L-Buthionine sulfoximine (BSO).
研究领域:
生物化学
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分析化学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2019
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IF 2.931
Qiujuan Ma,Chunyan Wang,Yu Bai,Junhong Xu,Juan Zhang,Zhengkai Li,Xiaoyu Guo
Hypochlorous acid (HClO) is an important reactive oxygen species (ROS) and plays a significant role in living organisms. Highly selective and lysosome-targetable probes for sensing hypochlorous acid are rare. In this article, we designed and prepared a new lysosome-targeting and ratiometric fluorescent probe for monitoring the levels of hypochlorous acid. 4-Aminonaphthalimide was chosen as the fluorescent group and (2-aminoethyl) thiourea group was used as a specific recognition group for HClO. A morpholine unit was employed as a lysosome-targeting group. In the absence of HClO the probe underwent intramolecular charge transfer (ICT) and showed a green emission. When excess HClO is present, the ICT process was interrupted which caused a 57 nm blue-shift of fluorescence emission from 533 nm to 476 nm. The ratiometric fluorescent probe showed outstanding selectivity toward HClO over other various bioactive species. Furthermore, the ratiometric fluorescent probe exhibited rapid response time and ability of working in a wide pH range. The linear response of I 476nm /I 533nm toward HClO was obtained in a concentration range of HClO from 1.0 × 10 −6 to 1.0 × 10 −4 mol·L −1 . The detection limit was estimated to be 8.0 × 10 −7 mol·L −1 for HClO. Moreover, the probe showed a perfect lysosome-targeting ability, and has been successfully used to the confocal imaging of HClO in lysosomes of HepG2 cells with little cell toxicity. All of such good properties illustrated that it could be applied to determine HClO at lysosomes in living cells.
研究领域:
生物化学
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细胞生物学
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DOI
PubMed
高影响因子
Analytical Methods
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2019
·Vol. 11
·期 13
·1751-1756
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IF 2.378
Kangming Xiong,Fangjun Huo,Yongbin Zhang,Ying Wen,Caixia Yin
Hypochlorous acid (HClO), as one of the reactive oxygen species (ROS), is very important to the human immune system. Although many hypochlorous acid fluorescent probes have been developed, most of them are not suitable for detecting endogenous HClO due to their own limitations such as a slow response time and short emission wavelength. In this work, we designed a near-infrared ratiometric (2.1 to 15.3, I608/I735) fluorescent probe with a quick response (20 s) based on cyanine polyene derivatives. The system could detect endogenous hypochlorous acid in HepG-2 cells. It is significant that the color change in the measurement process makes the probe applicable to the determination of hypochlorous acid content in an 84 disinfectant and other actual samples.
研究领域:
生物化学
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医学检测
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分析化学
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DOI
高影响因子
JOURNAL OF LUMINESCENCE
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2017
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IF 2.686
Jianfeng Liu,Ying Qian
In this work, a novel pyridylvinyl rhodamine-naphthalimide ratiometric fluorescent probe , In this work, a novel pyridylvinyl rhodamine-naphthalimide ratiometric fluorescent probe, RNVP , was synthesized and characterized by 1 H NMR, 13 C NMR and HRMS techniques. This probe shows high selectivity and sensitivity towards Fe 3+ and Hg 2+ . At a low concentration of Fe 3+ /Hg 2+ , a new emission peak appeared at 581 nm and enhanced significantly along with naked-eye sensitive color changes, which spirolactam-ring moiety of rhodamine was converted to the open-ring form in the presence of Fe 3+ /Hg 2+ . Further addition of Fe 3+ /Hg 2+ , the fluorescence intensity at 438 nm was enhanced rapidly, which attributed to the suppression of the PET process. The proposed sensing system of probe RNVP represented dual-channel and segment detection behavior, with the fluorescence “turn-On or Off” response and a very low detection limit of 2.72×10 −8 M and 9.08×10 −8 M, and the dissociation constant (Kd) were calculated to be 4.95×10 −7 M 3/2 and 6.68×10 −8 M 3/2 , respectively. RNVP also quick responded linearly to the acidic solution and monitor the pH change in the range of 2.92–4.5, as well as excellent reversibility.
研究领域:
分析化学
·
材料科学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
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2021
·
IF 6.558
Zhe Qiao,Zilian Jiang,Qiufen Luo,Hongfang Zhang,Jianbin Zheng
Labeling with redox reporter is often required in developing electrochemical bioassay for most proteins or nucleic acid biomarkers. Herein, a label-free ratiometric immunosensing platform is firstly developed by integrating the antibody-conjugated nanochannels with a smart modified electrode . The electrode modifier is the composite of C 60 , tetraoctylammonium bromide (TOA + ) and Prussian blue (PB). Cyclic voltammograms of the ultimate C 60 -TOA + /PB modified electrode exhibited two pairs of peaks at 0.15 V and −0.13 V, ascribing to the redox of PB and C 60 , respectively. With the addition of K 3 [Fe(CN) 6 ] in the electrolyte solution , the peaks of PB decreased due to the adsorption of [Fe(CN) 6 ] 3− while the peaks of C 60 increased because of the formation of the ternary complex (TC) C 60 -TOA + -[Fe(CN) 6 ] 3− . As a result, the peak current ratio I PB / I TC decreased gradually with the increment of the concentration of [Fe(CN) 6 ] 3− . For the nanochannels-based immunosensing platform, the steric hindrance of the bioconjugated nanochannels varied with the loading amount of the target CA125, and thus [Fe(CN) 6 ] 3− passing through the channels was quantitatively affected. And the higher CA125 level was, the less [Fe(CN) 6 ] 3− concentration was. And thus, the ratio I PB / I TC monitored at the C 60 -TOA + /PB modified electrode increased with the increase of the concentration of CA125. The ratiometric immunoassay featured a linear calibration range from 1.0 U mL −1 to 100 U mL −1 with a low detection limit of 0.86 U mL −1 . In addition, the ratiometric immunosensing platform demonstrated good specificity and stability as well as acceptable accuracy in overcoming the effect of electrode passivation which was an inherent problem of electroanalysis.
研究领域:
生物传感器
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医学检测
·
电化学分析
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2018
·
IF 5.667
Xin Hai,Yiting Wang,Xiaoyao Hao,Xuwei Chen,Jianhua Wang
Folic acid (FA) encapsulated graphene quantum dots (FA-GQDs) were prepared via a one-pot microwaved alkali cutting method, using graphene oxide (GO) and FA as raw material. TEM and AFM images revealed that FA was successfully cross-linked on the surface of GQDs under alkali circumstance, giving rising to stable and homogeneous FA-GQDs with size of about 33.6 nm. XPS and FT-IR confirmed that there are abundant hydroxyl, carboxyl and amino groups on the encapsulated FA-GQDs. The as-prepared FA-GQDs possessed the fluorescence behaviors of multi-excitation and emission sites, and exhibited sensitive response to the pH variation within the pH range of 3–9, making it excellent fluorescence probe for pH sensing in cell lysate and environmental water samples. The broad emission with pH-sensitive characteristic and low toxicity of FA-GQDs also provided an alternative for the specific multicolor imaging and sensing of pH in living cells.
研究领域:
生物化学
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纳米医学
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分析化学
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产品关联
DOI
高影响因子
DYES AND PIGMENTS
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2019
·
IF 4.018
Wei Wang,Xian-Jing Han,Jin-Ting Liu,Jun-Ying Miao,Bao-Xiang Zhao
A new ratiometric fluorescence probe (named CPC) with high selectivity and sensitivity toward bisulfite (HSO 3 − ) based on a FRET platform was developed. Probe CPC was constituted by a coumarin-carbazole dyad with a large (pseudo) Stokes shift (197 nm). The limit of detection was measured to be as low as 18 nM and the signal-to-background ratio of fluorescence intensity was up to 73-folds, implying a high sensitivity of probe CPC toward HSO 3 − . What's more, probe CPC was successfully applied to the detection of endogenous HSO 3 − and specific imaging of lipid droplets in HepG2 cells.
研究领域:
生物化学
·
细胞生物学
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DOI
高影响因子
TETRAHEDRON
·
2020
·
IF 2.233
Jian Chen,Kai Chen,Baichuan Han,Yunting Xue,Wanjuan Chen,Zhenzhong Gao,Xianfeng Hou
A single-fluorophore-based ratiometric fluorescent probe based on anthracene carboximide was developed for detection of formaldehyde. In the presence of formaldehyde, the fluorescence emission would change from red to yellow-green, and the probe showed excellent sensitivity, selectivity and anti-interference. Furthermore, the response mechanism was illustrated to be that change in fluorescent signal is resulted from variation of substituent group on the 6th position of anthracene ring. A test paper was developed by loading probe onto filter paper strip. With this test paper, the detection of gaseous formaldehyde could be carried out conveniently, visually and quickly. 2009 Elsevier Ltd. All rights reserved.
研究领域:
分析化学
·
环境科学
·
生物医学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2016
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IF 4.758
Li Wang,Coucong Gong,Yuan Shen,Wenhui Ye,Mengli Xu,Yonghai Song
In this work, a novel ascorbic acid (AA) ratiometric biosensor was prepared by using the PCN-333 (Al) metal-organic frameworks (MOFs) (PCN stands for porous coordination network) to encapsulate Ketjen black (KB) as catalyst for catalyzing oxidation of AA and thionine (Thi) as an internal reference signal simultaneously. The encapsulation of KB and Thi in the pores of PCN-333 (Al) MOFs not only improved efficiency of KB and Thi greatly because PCN-333 (Al) MOFs could effectively avoid the cohesion or aggregation of KB and Thi on electrode surface but also enhanced the stability of biosensors because they were immobilized in the pore firmly. Furthermore, PCN-333 (Al) MOFs could also selectively accumulate target analytes into their pores to enhance the selectivity of biosensors. The oxidation peak current of AA catalyzed by KB at −0.05 V increased with the increasing concentration of AA, while the oxidation peak current of Thi at −0.24 V kept constant, which resulted in a novel ratiometric biosensor for AA detection. The ratiometric biosensor exhibited a wider linear range from 14.1 ± 0.2 to (5.5 ± 0.1) х10 3 μM (R 2 = 0.998) and a lower detection limit of 4.6 ± 0.1 μM with high accuracy, selectivity, reproducibility and sensitivity. The ratiometric electrochemical approach is not only a new method for AA detection but also opens a new way for sensitive detection of other analytes.
研究领域:
分析化学
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生物传感技术
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DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2017
·
IF 3.387
Huanhuan Xu,Lihe Yan,Vanthan Nguyen,Yang Yu,Yanmin Xu
Nitrogen-doped carbon nanodots (CDs) are synthesized by one-step femtosecond laser ablation of graphite powder in aminotoluene at room temperature. The as-prepared CDs have the average diameter of 2.87 nm and possess an excitation-independent emission covering nearly the whole visible light region at a single excitation wavelength. The X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR) analysis indicate that there are a huge number of multiple oxygen groups and amine groups on the surface of the CDs. As their different fluorescence peaks originated from different emission surface groups on the nanodots show different pH dependence, these CDs can be used for ratiometric pH sensing.
研究领域:
材料科学
·
分析化学
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Taotao Li,Xinyi Li,Yu Zheng,Pan Zhu,Cheng Zhang,Kui Zhang,Jing-Juan Xu
Employing long-lived luminescent materials to design a chemical sensing platform can eliminate real-time excitation and background fluorescence. However, the realization of long-lived emissions in aqueous media was limited to transition-metal complexes, doped quantum dots, organic crystals, and inorganic persistent phosphors, which suffer from the drawbacks of large size, expensive elements, and poor dispersibility. In this work, phosphorescent carbon dots (CDs) were covalently immobilized in a silica matrix (CDs@SiO2) to achieve afterglow emission in an aqueous dispersion. CDs@SiO2 with long lifetime (∼1.6 s) was utilized as an energy donor to fabricate nonradiative energy transfer systems with various organic dyes through the surface micelle self-assembly method. Benefiting from the high energy transfer efficiency between CDs@SiO2 and organic dyes, multicolor afterglow emissions were successfully obtained in aqueous media. As a proof of concept, a ratiometric phosphorescent probe using CDs@SiO2 as a donor and Hg2+-responsive rhodamine derivative as an acceptor was designed. Hg2+ triggered the energy transfer process between the donor–acceptor pair, leading to the sensitive detection of Hg2+ ions. The work presented here provides opportunities to develop chemical sensors with low background interferences and easily recognizable signals.
研究领域:
分析化学
·
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2022
·
IF 5.122
Zishuo Yi,Weiling Huang,Yan Xu
Dual-emitting ratiometric optical thermometers with the advantages of excellent self-calibration and high-sensitivity have become a research hot spot. Herein, a dual-emissive 4-methylumbelliferone encapsulated ZIF-8 (ZIF-8⊃4-MU) modified Zn 2 GeO 4 :Mn 2+ nanocomposite was simply prepared by adding Zn 2 GeO 4 :Mn 2+ nanorods into a methanol solution containing 4-methylumbelliferone (4-MU), zinc (II) nitrate hexahydrate, and 2-methylimidazole. Accompanying with the self-assembly of ZIF-8⊃4-MU, the binding affinity of Zn 2+ ions to the surface of Zn 2 GeO 4 :Mn 2+ nanorods enables the formation of ZIF-8⊃4-MU@Zn 2 GeO 4 :Mn 2+ nanocomposite. The photoluminescence violet-blue (420 nm) and green emissions (531 nm) of the nanocomposite can be ascribed to the two independent luminescent centers of 4-MU dyes confined in ZIF-8 and the characteristic transition of 4 T 1 - 6 A 1 for Mn 2+ ions doped in Zn 2 GeO 4 , respectively. The fluorescent intensity ratios (FIRs) of 4-MU to Mn 2+ (I 420 nm /I 531 nm ) showed excellent self-calibrating temperature monitoring functions with a maximum relative sensitivity (S r ) of 1.82%· o C −1 and stable reusability in the temperature range of 20–100 °C. Also, the ZIF-8⊃4-MU@Zn 2 GeO 4 :Mn 2+ hybridized polyvinyl alcohol (PVA) transparent film showed a similar multi-emitting behavior, as well as good processability and tensile properties . Benefiting from the excitation-dependent emitting property of ZIF-8⊃4-MU@Zn 2 GeO 4 :Mn 2+ , a multiple optical encryption of digital information was successfully realized based on a two-dimensional code, achieving an enhanced security level for preventing counterfeiting. This proof-of-concept study offers a feasible assembled way in fabricating multi-mode luminescent nanocomposites for ratiometric temperature sensing and information encryption .
研究领域:
材料科学
·
光学
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DOI
高影响因子
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