CHEMISTRY-A EUROPEAN JOURNAL
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2014
·Vol. 20
·期 37
·11760-11767
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IF 5.696
Ping Li,Shan Zhang,Nannan Fan,Haibin Xiao,Wen Zhang,Wei Zhang,Hui Wang,Bo Tang
Graphical All aglow! A new fluorescence ratio imaging probe, 6-methoxyquinolinium–dansyl (MQ-DS), with single excitation/dual maximum emission, responds precisely and instantaneously to changes of Cl − concentrations in lysosomes (see picture). It is utilized successfully to quantitatively visualize fluctuations of Cl − in live cells and in vivo. Fluorescence ratio imaging is currently being used to quantitatively detect biologically active molecules in biosystems; however, two excitations of most existing fluorescent ratiometric probes account for cumbersome operating conditions for imaging. Thus, a fluorescent ratiometric probe, 6-methoxyquinolinium–dansyl (MQ-DS), for Cl − with single excitation/dual maximum emission has been developed. MQ-DS can preferably localize into lysosomes and display excellent photostability. Upon excitation at a single wavelength, it responds precisely and instantaneously to changes in Cl − concentrations, and it can be conveniently utilized to implement real-time fluorescence ratio imaging to quantitatively track alterations in Cl − levels inside cells treated under various pH conditions, and also in zebrafish with acute wounds. The successful application of the new probe in bioimaging may greatly facilitate a complete understanding of the physiological functions of Cl − .
研究领域:
生物化学
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细胞生物学
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分子影像学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Jin Peng,Xianfeng Hou,Fang Zeng,Shuizhu Wu
Drug-induced organ damages have been considered as a grave problem regarding public health; hence effective method for in vivo detection of drug-induced organ damages is of great significance. Herein we developed a ratiometric fluorescent nanoprobe (NPs-A), which was prepared by loading the probe molecules into phospholipid bilayer, for assaying hydrogen peroxide (H 2 O 2 , an organ damage biomarker) level in vivo. The photophysical behavior of the probe molecule depends on the electron-withdrawing ability of the group at the 6- position of anthracene ring, on which the recognition moiety for hydrogen peroxide (dicarbonyl coupled with nitrophenyl, referred to as nitrophenyl-dicarbonyl) was introduced. Upon the reaction of the probe with H 2 O 2 , nitrophenyl-dicarbonyl group transforms into carboxyl group, and due to the variation of the electron-withdrawing ability of the 6th substituent , the fluorescent properties of the probe molecule alters accordingly, thus ensuring the ratiometric detection for H 2 O 2 with high selectivity with the detection limit of 0.49 μM. In addition, the nanoprobe (NPs-A) was applied for cell and in vivo imaging applications; and the results indicate that it can detect and track the level of H 2 O 2 in living cells and to monitor and spatially map endogenous H 2 O 2 levels in a drug-induced organ damage model of zebrafish.
研究领域:
生物医学工程
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分析化学
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毒理学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
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2020
·Vol. 187
·期 9
·1-10
·
IF 6.232
Deng Zhiwei,Li Xiufang,Liu Hui,He Yao,Zhang Jie,Yuan Jing,Gao Peiru,Jiang Xuling,Yang Yanjing,Zhong Shian
A ratiometric fluorescence assay for glutathione (GSH) was developed. The novel assay is based on a nanoprobe composed of manganese dioxide nanosheets (MnO 2 NS) and dual-emission carbon dots (de-CDs) with intrinsic GSH-response property. After construction of the nanoprobe, two emission peaks of de-CDs were suppressed to varying degrees by MnO 2 NS. The suppression was relieved and the two emission peaks recovered proportionally when MnO 2 NS was decomposed by GSH, thus realizing the ratiometric assay for micromolar GSH. The intrinsic responsiveness of de-CDs to millimolar GSH broadens the analytical range of the nanoprobe. An appropriate precursor, calcon-carboxylic acid, was screened out to synthesize de-CDs via one-step hydrothermal treatment. The de-CD@MnO 2 NS nanoprobe can measure GSH concentrations through the fluorescence intensity ratio between 435 and 516 nm excited at 365 nm. The range of response was from 1 μM to 10 mM and the detection limit reached 0.6 μM (3σ criterion). Benefiting from its good biocompatibility, the proposed nanoprobe has excellent applicability for intracellular GSH imaging. Graphical abstract Schematic representation of glutathione (GSH) ratiometric detection. The nanoprobe is prepared from dual-emission carbon dots (de-CDs) and manganese dioxide nanosheets (MnO 2 NS). GSH removes quenching effect by decomposing MnO 2 NS and induces intrinsic response of de-CDs, which realizes ratiometric detection.
研究领域:
分析化学
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纳米技术
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生物医学工程
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细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2022
·
IF 8.008
Sheng Chen,Jiayi Fan,Mengya Lv,Chenfeng Hua,Gaolin Liang,Shusheng Zhang
Hydrogen sulfide (H2S), as the third endogenous gasotransmitter, is closely associated with various physiological and pathological processes, whereas many aspects of its functions remain unclear. Effective tools for the accurate detection of H2S in living organisms are urgently needed. We herein reported an internal standard assisted surface-enhanced Raman scattering (SERS) nanoprobe for ratiometric detection of H2S in vitro and in living cells based on the reduction of nitros with H2S. This nanoprobe consists of an internal standard (4-mercaptobenzonitrile, MPBN) embedded core-molecule-shell Au nanoflower (Au@MPBN@Au) as the high plasmonic active SERS substrate and the 4-nitrothiophenol (4-NTP) molecule immobilized on the surface as the H2S recognition unit. With the addition of H2S, the nitros peak (1329 cm–1) decreased. Meanwhile, three obvious new peaks appeared at 1139, 1387, and 1433 cm–1, which were related to the vibration of the dimerized product 4,4′-dimercaptoazobisbenzene (DMAB) of 4-aminothiophenol (4-ATP). However, the peak intensity at 2223 cm–1 derived from MPBN was not influenced by the outer environment. Thus, the H2S level was able to be determined based on the ratio of two peak intensities (I1139/I2223) with a detection limit as low as 0.24 μM. Notably, we have proved that SERS nanoprobe Au@MPBN@Au@4-NTP could ratiometrically image both the endogenous and exogenous H2S in living cells. We anticipate that Au@MPBN@Au@4-NTP could be applied for the study of H2S-related physiological function in the future.
研究领域:
分析化学
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生物医学工程
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分子生物学
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生物成像
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2025
·
IF 7.7
Lixian Huang,Jinzhe Liang,Jiaqi Lu,Min He,Yidong Bin,Yanni Luo,Shulin Zhao,Shengqiang Hu,Liangliang Zhang
The integration of ratiometric fluorescence imaging in the second near-infrared (NIR-II) window with chemodynamic therapy (CDT) and photodynamic therapy (PDT) represents a promising strategy for the accurate diagnosis and efficient treatment of deep tumors. In this study, we developed a smart nanoprobe, D/UCNP-MB@SiO 2 - t AuCuNCs (D/UCMSA), with simultaneously triggering reactive oxygen species (ROS) and NIR-II fluorescence for enhanced chemodynamic/ photodynamic therapy and ratiometric imaging-guided tumor resection. Upon 980 nm laser irradiation, D/UCMSA emit up-converted light at 660 nm, which activates the loaded methylene blue (MB) to produce singlet oxygen ( 1 O 2 ) for PDT. Simultaneously, the down-converted emission at 1525 nm serves as a reference signal for ratiometric imaging. The D/UCMSA surface is decorated with copper-doped gold nanoclusters ( t AuCuNCs) stabilized by triphenylphosphine-3,3′,3′′-trisulfonic acid, which undergo ligand exchange with overexpressed glutathione in the tumor microenvironment. Upon 808 nm laser excitation, this ligand exchange process activates fluorescence emission at 1085 nm. The incorporation of copper endows t AuCuNCs with peroxidase- and catalase-like activities, enabling the catalytic conversion of hydrogen peroxide (H 2 O 2 ) into hydroxyl radicals (·OH) and oxygen (O 2 ) within the tumor microenvironment. This dual enzymatic activity amplifies the CDT efficacy in tumor treatment and reshapes the tumor microenvironment, providing sufficient O 2 for 1 O 2 generation during PDT and enhancing the efficiency of combined CDT/PDT. Following with orthotopic injection of the D/UCMSA, irradiation with 808 nm and 980 nm lasers successfully enabled precise tumor resection guided via ratiometric NIR-II fluorescence imaging, combined with effective CDT/PDT. This study demonstrates a novel approach to developing integrated nanoplatforms for accurate tumor diagnosis and efficient treatment.
研究领域:
生物医学工程
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纳米医学
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肿瘤学
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产品关联
DOI
高影响因子
Nanomaterials
·
2022
·Vol. 12
·期 24
·4478
·
IF 5.719
Qingyuan Wang,Zhizheng Zhang,Dehui Qiu,Xuanxiang Mao,Zhaoxi Zhou,Tiansong Xia,Jifu Wei,Qiang Ding,Xiaobo Zhang
Just-in-time evaluation of drug resistance in situ will greatly facilitate the achievement of precision cancer therapy. The rapid elevation of reactive oxygen species (ROS) is the key to chemotherapy. Hence, suppressed ROS production is an important marker for chemotherapy drug resistance. Herein, a NIR-II emission smart nanoprobe ([email protected], consisting of a lanthanide-doped nanoparticle (LnNP) core and metal-organic framework shell (ZIF8)) is constructed for drug delivery and in vivo NIR-II ratiometric imaging of ROS for tumor drug resistance evaluation. The drug-loaded nanoprobes release therapeutic substances for chemotherapy in the acidic tumor tissue. As the level of ROS increases, the LnNPs shows responsively descending fluorescence intensity at 1550 nm excited by 980 nm (F1550, 980Ex), while the fluorescence of the LnNPs at 1060 nm excited by 808 nm (F1060, 808Ex) is stable. Due to the ratiometric F1550, 980Ex/F1060, 808Ex value exhibiting a linear relationship with ROS concentration, NIR-II imaging results of ROS change based on this ratio can be an important basis for determining tumor drug resistance. As the chemotherapy and resistance evaluation are explored continuously in situ, the ratiometric imaging identifies drug resistance successfully within 24 h, which can greatly improve the timeliness of accurate treatment.Keywords:lanthanide doped nanoparticle;ZIF8;NIR-II ratiometric imaging;smart drug delivery system;tumor drug resistance evaluation
研究领域:
癌症治疗
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纳米技术
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生物医学工程
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药学
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成像技术
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL RESEARCH
·
2025
·
IF 7.7
Wei Bi,Chuanli Ren,Yuxin Xiao,Feng Wang,Qingmao Long,Yanfei Lin,Xinjie Yang,Li Liu,Lianpeng Zhang,Guanben Du
Cellulose is the most abundant renewable biomass resource on Earth, with good biodegradability and biocompatibility. In this study, a novel cellulose-based near-infrared fluorescent probe MN@NIR for ClO − detection was developed by amination modification of microcrystalline cellulose (MCC), followed by the introduction of naphthalimide fluorophores and dicyanoisophorone groups. The probe MN@NIR exhibits excellent fluorescence properties with dual-emission peaks at 543 nm and 690 nm, the latter falling within the near-infrared (NIR) window. It shows highly specific “ratiometric” and “colorimetric” fluorescence responses to ClO − , with the fluorescence color gradually changing from red to blue as the ClO − concentration increases. Notably, the probe demonstrates a rapid response time (2 min) and excellent selectivity and anti-interference properties. The fluorescence film prepared from the probe shows outstanding specificity for ClO − and has been successfully applied to the visual monitoring of ClO − in water samples. Furthermore, this study combines smartphone image analysis technology to develop a G/B color ratio- ClO − monitoring platform, providing a new solution for the quantitative analysis of ClO − concentrations in water and on-site rapid detection.
研究领域:
分析化学
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材料科学
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环境监测
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生物成像
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传感器与检测技术
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Rong Sheng Li,Jiahui Liu,Yuan Yan,Chuanyang Su,Tiancheng Lai,Yunwen Liao,Yuan Fang Li,Na Li,Cheng Zhi Huang
Förster resonance energy transfer (FRET) from fluorescent nanoparticles to fluorescent dyes is an attractive approach for bioanalysis in living cells. However, the luminescence of the nanoparticle donor/acceptor has not been effectively used to produce highly efficient FRET because the distance between the energy donor and energy acceptor is often larger than the effective FRET radius (about 10 nm) and the uncontrolled rotational and translational diffusion of luminophores. Here, we develop an aggregation-enhanced energy transfer strategy that can overcome the impedance for effective energy transfer. The functional nanoprobes, named TPP-CDs-FITC, are carbon dots (CDs) functionalized with triphenylphosphine (TPP) and ∼117 fluorescein 5-isothiocyanate (FITC) on the surface. In dispersed solution, the 3.8 nm TPP-CDs-FITC show weak FRET efficiency (15.4%). After TPP-instructed mitochondrial targeting, enhanced FRET efficiency (53.2%) is induced due to the aggregation of TPP-CDs-FITC selectively triggered by adenosine triphosphate (ATP) in the mitochondria. The enhanced FRET efficiency can be attributed to the joint effect of the augment of numbers of FITC acceptors within 10 nm from dispersed 117 to aggregated 5499 and the restricted rotational and translational motions of TPP-CDs donors and FITC acceptors. Ultimately, we successfully observe the fluctuations of ATP levels in the mitochondria using the aggregation-enhanced energy transfer strategy of the TPP-CDs-FITC nanodevice.
研究领域:
生物物理学
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生物化学
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细胞生物学
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纳米技术
✓ 已验证
DOI
PubMed
高影响因子
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