SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Yinghua Guo,Xinru Wang,Yan Feng,Xiao Luo,Xiangming Meng,Xuhong Qian,Youjun Yang
Autophagy is a physiological process governing proteostasis and implicated in many diseases. Enhanced ROS levels was known to be associated with lysosomal autophagy and therefore a lyso-specific ROS probe could find applications in autophagy related studies. Following the probe-design principle of bridging-group modification, we previously developed a ROS probe capable of detect a broader range of reactive species than the existing probes. Additional merits of the probe include ratiometry-capability, minimal probe-product spectral crosstalk, resistance toward autoxidation at lysosomal acidity, and structural simplicity. It is the aim of this manuscript to render this probe lyso-specificity and examine its potentials to monitor autophagy-related ROS generation in HeLa cells treated by H 2 O 2 or 6-OHDA.
研究领域:
细胞生物学
·
生物化学
·
医学研究
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Meng Li,Xiaoning Li,Mengwen Xu,Bowen Liu,Mengqing Yang,Zhijun Chen,Tianchu Gao,Tony D. James,Lidong Wang,Huining Xiao
Mercury ions are one of the most toxic heavy metals and as such they can cause serious risk to the human body and environmental ecosystem. To effectively detect and remove Hg 2+ from contaminated water, a new cellulose-based fluorescent hydrogel has been fabricated using a ratiometric probe with carbon dots as energy donor and rhodamine moiety as energy acceptor (CDs-Rho). The probe exhibits a sensitive and linear response to Hg 2+ over a wide range from 0–100 μM with a limit detection of 2.19 × 10 −9 M and exhibits high selectivity for Hg 2+ over other cations. In addition, a series of cellulose-based fluorescent hydrogel slices containing CDs-Rho are continuously prepared using microtomy of a hydrogel, facilitating the large-scale fabrication of functionalized hydrogel slices with controlled thickness. The cellulose-based CDs-Rho (CCR) hydrogel exhibits good sensitivity and excellent adsorption capacity for Hg 2+ with ~95% removal efficiency, meeting the requirements for sewage discharge. Moreover, the purified water is successfully used for cell culture and animal growth, demonstrating excellent biocompatibility. Our approach is expected to offer a novel concept for the construction of biocompatible fluorescent hydrogels for the detection of various metal ions and removal by simply swapping the current probe with suitable replacements for a variety of relevant applications.
研究领域:
环境科学
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2021
·
IF 8.198
Xiaoyu Wang,Yishui Wang,Wei Pan,Jinping Wang,Xiaobo Sun
It is difficult to quantitatively explain the effects of intracellular conditions on bioreactions in cells because direct measurements in cells are not easily achieved. In this work, a relationship between the biosynthesis of intracellular polysaccharide and intracellular pH inside fungal cells was established by quantitatively determining them using proper methods, respectively. A fluorescent ratiometric pH probe was designed and synthesized for the detection of intracellular pH by conjugating a pH-sensitive moiety and pH-insensitive carbon dots (CDs) into one system. The intracellular pH values and intracellular polysaccharide contents inside the mycelia of Pholiota adipose fungus incubated under different conditions were individually analyzed by the ratiometric pH probe and a phenol-sulfuric acid method. Thereafter, a relationship was established using the content of polysaccharide in mycelia as ordinates and intracellular pH values as abscissa. As can be seen from the relationship, intracellular pH in the range 7.10–7.25 inside Pholiota adipose mycelia is the most suitable condition for the biosynthesis of intracellular polysaccharide.
研究领域:
生物化学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
ACS Sensors
·
2024
·
IF 8.2
Lin He,Yan Zhou,Min Zhang,Mingjian Chen,Yuchen Wu,Lanlin Qi,Lamei Liu,Bin Zhang,Xiaohai Yang,Xiaoxiao He,Kemin Wang
Detection of slight pH changes in skin interstitial fluid (ISF) is crucial yet challenging for studying pathological processes and understanding personal health conditions. In this work, we construct an i-motif DNA based fluorescent ratiometric microneedle sensing patch (IFR-pH MN patch) strategy that enables minimally invasive, high-resolution, and sensitive transdermal monitoring of small pH variations in ISF. The IFR-pH MN patch with advanced integration of both ISF sampling and pH sensing was fabricated from the cross-linking of gelatin methacryloyl and methacrylated hyaluronic acid, wrapping with pH-sensitive hairpin-containing i-motif DNA based fluorescent ratiometric probes in the matrix. Because it is mechanically robust for skin penetration and has high swelling ability, the IFR-pH MN patch could be quickly extracted as sufficient liquid from agarose gel (∼56.4 μL in 10 min). Benefiting from conformation changes of the hairpin-containing i-motif DNA under pH variation and ratiometric fluorescence signal readout, the IFR-pH MN patch could quantitate pH over a small range between pH 6.2 and 6.9 with an accuracy of 0.2 pH units in the mimic skin model. Furthermore, in vivo testing on wound and tumor mouse models indicated the ability of the biocompatible IFR-pH MN patch to penetrate the skin for obtaining transdermal pH values, demonstrating the potential applications in monitoring and intervention of pathological states.
研究领域:
生物医学工程
·
分子生物学
·
传感技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Small
·
2025
·
IF 12.1
Lixian Huang,Fei Lv,Yidong Bin,Jingjin Zhao,Caiying Li,Shulin Zhao,Shengqiang Hu,Liangliang Zhang
Hydrogen sulfide (H 2 S) is a vital gaseous signaling molecule that plays a central role in various physiological and pathological processes. Given the complementary advantages of fluorescence (FL) and photoacoustic (PA) imaging, there is a growing demand for dual-ratiometric probes that enable precise in vivo monitoring of H 2 S levels. In this study, the use of 2-mercapto-1,3,4-thiadiazole (MTD) as a novel recognition group of H 2 S is presented for the first time, following conjugation with cyanine dyes to obtain a new PA probe Cy-MTD. To achieve dual-ratiometric imaging, Cy-MTD is incorporated into down-conversion nanoparticle (DCNP), resulting in the creation of a pioneering NIR-II FL/NIR-I PA dual-ratiometric nanoprobe DCNP@Cy-MTD. Cy-MTD undergoes the blueshift in absorption from 840 to 670 nm after reaction with H 2 S, enabling NIR-I ratiometric PA imaging of H 2 S by measuring the ratio of PA signal at 670 and 840 nm (PA 670 /PA 840 ). In addition, due to the strong absorption of Cy-MTD ≈840 nm and the overlapping between the absorption spectrum of Cy-MTD and 808 nm excitation band of DCNP, the 808 nm-excited FL emission (F 1550 nm,808Ex ) of DCNP in DCNP@Cy-MTD nanoprobe is quenched through the competitive absorption, while it is restored upon the interaction with H 2 S because of the blueshift in absorption of Cy-MTD. Using the stable FL emission of DCNP under 980 nm excitation (F 1550 nm,980Ex ) as the reference signal, NIR-II ratiometric FL imaging (F 1550 nm,808Ex /F 1550 nm,980Ex ) of H 2 S is achieved. The dual-ratiometric response features of the DCNP@Cy-MTD nanoprobe offer a significant advancement over traditional single-signal or single-modality imaging techniques. By providing enhanced accuracy and reliability, this probe allows for the diagnosis of hepatitis by monitoring the H 2 S, surpassing the capabilities of conventional histopathological methods, which provides a new way for more effective diagnostic strategies for liver diseases.
研究领域:
生物医学工程
·
分子影像学
·
肝脏疾病研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEDICINAL CHEMISTRY
·
2024
·
IF 6.8
Tingting Han,Ye Sun,Chao Zhao,Hai-Yan Wang,Hui Yu,Yi Liu
Rheumatoid arthritis (RA) is a destructive autoimmune disease, where nitric oxide (NO) is closely implicated in the inflammatory processes of RA. Therefore, direct visualization of NO is essential to assess the pathological changes in RA. Herein, a mitochondrial-targeted near-infrared ratiometric fluorescent probe (NFL-NH2), based on the intramolecular charge transfer effect, was synthesized and applied to monitor the changes of NO content in early RA. Specially, probe NFL-NH2 showed a 44-fold fluorescent intensity ratio (I705/I780) response toward NO with a detection limit of 0.536 nM, enabling qualitative and quantitative analysis of NO. Additionally, NFL-NH2 can accurately target mitochondria and sensitively detect exogenous and endogenous NO in RAW 264.7 cells. Notably, in vivo RA monitoring assays demonstrated that NFL-NH2 can rapidly detect NO levels associated with the inflammatory damage degree in RA mice models by ratiometric fluorescence imaging. These results validate that NFL-NH2 holds significant potential for diagnosing NO-mediated RA diseases.
研究领域:
生物医学工程
·
药理学
·
免疫学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Xu Yu,Chun-Xia Zhang,Lening Zhang,Ya-Rong Xue,Hong-Wei Li,Yuqing Wu
Förster resonance energy transfer (FRET) is a powerful tool to study single molecular conformation and/or distance changes between two fluorophores, which is mainly depended on the combinations and spatial distance between the donor and acceptor. The present study has built a FRET assembly by using gold nanoclusters capped by glutathione (AuNCs@GSH) and carbon dots (CDs) prepared by polyethyleneimine (PEI) through supramolecular interactions. The assembly is characterized in detail by employing Zeta potential, UV–vis absorption and fluorescence spectra, the luminescent decay curves and TEM images, which indicate the AuNCs are adhered to the surface of CDs. In addition, the transfer efficiency between them has been calculated to be 65.7%, which is sufficient enough for practical applications, and particularly, the quantum yield (QY) of AuNCs@GSH is increased from 3.07% to 5.35% via the assembly. Moreover, the FRET assembly is used as a ratiometric probe for cysteine detection in showing a limit of detection (LOD) at 100 nM, a very high selectivity to cysteine among amino acids as well as a strong anti-interference on them, especially, it was feasible for cysteine detection in fetal calf serum, which showed very high potential in the diagnose of cysteine-related disease.
研究领域:
生物化学
·
纳米生物学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYST
·
2022
·Vol. 147
·期 17
·3867-3875
·
IF 5.227
Fangfang Yu,Yihan Wang,Tengfei Liu,Xiaohui Liu,Hui Jiang,Xuemei Wang
Hypochlorite plays a vital role in biological systems and our daily life. The rapid and convenient detection of hypochlorite is imperative and significant for disease treatment and human health. In this work, EY/UiO-66-NH2 (EY = eosin Y) was prepared through a hydrothermal process and could be applied to the detection and bioimaging of hypochlorite as a self-calibrating sensing nanoprobe. EY/UiO-66-NH2 features two emissions at 432 nm and 533 nm, and the emission intensity of 533 nm is enhanced with increasing ClO− concentration. EY/UiO-66-NH2 could be utilized as a ratiometric fluorescence sensor of ClO−. The linear range of EY/UiO-66-NH2 towards ClO− is 0.1–200 μM and its detection limit is 46.4 nM. In comparison with previously reported probes for ClO−, EY/UiO-66-NH2 has the advantages of a wide linear range, low detection limit, turn-on fluorescence and ratiometric response. This work provides a new method for ClO− detection in living cells.
研究领域:
分析化学
·
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2024
·
IF 4.1
Nan Zhang,Hongwei Zhao,Huan Xia,Jiayue Zhou,Liwen Zhang,Na Sai
Herein, an optical ratios probe with reflection (RE) and fluorescence (FL), named as RE-FL ratios probe, has been developed for the ratiometric detection of glucose (GLU). This probe was prepared through self-assembling monodisperse colloids into opal photonic crystal and immobilizing fluorescence imidazolium ionic liquids as molecular recognition elements. So there was a periodic three-dimensional face-centered cubic arrayed structure and emissions of strong RE and FL in the probe, which allowed dual optical detection of the target analyte. From the results, it was learned that RE and FL emissions of RE-FL ratios probe were decreased when an increase of GLU concentration, and the resulting ratiometric optical changes can be quantitatively correlated to GLU concentration from 1 to 64 mM. The detection of probe was characterized by simple operation, stability, reusability and glucose oxidase independence. Furthermore, the probe successfully used to detect GLU in the real serum sample and showed great stability compared with the only FL pattern. Different types of appropriated recognition elements are expected to be introduced into opal photonic crystals to construct variety ratiometric optical probes in the wide of applications.
研究领域:
生物化学
·
医学诊断
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Polymer Chemistry
·
2017
·Vol. 8
·期 7
·1138-1145
·
IF 5.375
Nianqi An,Qiang Zhang,Jing Wang,Cui Liu,Luqing Shi,Lihua Liu,Ludi Deng,Yan Lu
This paper reports a new ratiometric probe for the detection of adenosine 5′-triphosphate (ATP) that is based on the binding-induced modulation of fluorescence resonance energy transfer (FRET) coupled with the aggregation-caused quenching (ACQ) sensing mechanism. The introduction of coumarin fluorophores as FRET donors into the side-chain of polythiophene (PT) as the FRET acceptor provides the ratiometric probe. Upon addition of ATP, the formation of a polymer/ATP complex leads to the conformational changes of the PT backbone from a random coil to the planar conformation and aggregation. As such, the ratiometric ATP detection can be achieved by taking advantage of spectral overlap changes to modulate the FRET efficiency from coumarin fluorophores to the PT backbone before and after the addition of ATP, coupled with ACQ. The probe exhibited a clear seesaw-type dual-emission signal change upon binding with strong affinity (Kapp = 3.12 × 104 M−1) to ATP in aqueous solution, whereas no obvious emission changes were observed with ADP, AMP or various other anions. The probe can also be utilized for naked-eye detection of ATP and real-time monitoring of the ATP hydrolysis reaction by apyrase under physiological pH conditions.
研究领域:
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 9
·3043-3055
·
IF 4.478
Liang Yu-Qi,Wu Xiao-Yi,Zeng Jia-Yu,Wu Yan-Ni,Lai Jia-Ping,Sun Hui
The novel dual-emission carbon dots (DECDs) for highly selective and sensitive recognition of chlortetracycline (CTC) and cell imaging were synthesized successfully by one-step synthesis. The obtained DECDs possessed two fluorescence peaks (345 nm and 450 nm) and showed specific response to CTC, resulting in a decrease in fluorescence intensity at 345 nm, a blue shift, and an increase in fluorescence intensity at 450 nm. The obtained DECDs exhibited highly selective response to CTC and not to its analogues, such as tetracycline, doxycycline, and oxytetracycline. Thus, an excellent ratiometric probe for the detection of CTC was fabricated successfully and used for the detection of CTC in real samples with the detection limit (LOD) of 16.45 nM. More importantly, the DECDs were used for quantitative detection of CTC in living cells, which demonstrated excellent biocompatibility and broad prospects in biomedicine application. Finally, the excellent selectivity of DECDs toward CTC was attributed to the FRET mechanism and the formation of complexes. Graphical abstract
研究领域:
分析化学
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2021
·
IF 4.889
Yingying Zhang,Yanliang Zhao,Bo Song,Chaobo Huang
Acidic pH is an important physiological parameter in environment and biological systems. Thus, designing a probe ( Hcy-F ) with good characters to monitor acidic pH is really important. Herein, a small molecular water-soluble probe is designed based on indole with high selectivity and sensitivity for detection the acidic pH in aqueous solution and living cells. When pH variation of aqueous is detected by probe, an obvious ratiometric and good linear response behavior are found in UV–vis absorption spectrum (R 2 = 0.9973, A 434 nm / A 371 nm = 6.9929–4.2231 pH) and fluorescence emission spectrum (R 2 = 0.9987, I 571 nm / I 514 nm = 7.2935–2.981 pH), which further proved the feasibility of Hcy-F to monitor the change of acidic pH in aqueous, respectively. Furthermore, Hcy-F is successfully applied for monitoring pH variation in living cells in dual channels (green and red) with higher cell survival rate, indicating the probe has great potential for pH imaging in biological applications .
研究领域:
分析化学
·
生物化学
·
细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）