MOLECULES
·
2020
·Vol. 25
·期 12
·2826
·
IF 3.267
Jun Mei,Feng Liu,Shiyuan Fang,Weiqing Lan,Jing Xie
The effects of modified atmosphere packaging (MAP) in combination with superchilling (−1.3 °C) on the physicochemical properties, flavor retention, and organoleptic evaluation of turbot samples were investigated during 27 days storage. Results showed that high-CO2packaging (70% or 60% CO2) combined with superchilling could reduce the productions of off-flavor compounds, including total volatile basic nitrogen (TVB-N) and ATP-related compounds. Twenty-four volatile organic compounds were determined by gas chromatography–mass spectrometry (GC/MS) during storage, including eight alcohols, 11 aldehydes, and five ketones. The relative content of off-odor volatiles, such as 1-octen-3-ol, 1-penten-3-ol, (E)-2-octenal, octanal, and 2,3-octanedione, was also reduced by high-CO2packaging during superchilling storage. Further, 60% CO2/10% O2/30% N2with superchilling (−1.3 °C) could retard the water migration on the basis of the water holding capacity, low field NMR, and MRI results, and maintain the quality of turbot according to organoleptic evaluation results during storageKeywords:turbot;modified atmosphere packaging;superchilling;shelf life;quality
研究领域:
食品科学
·
水产学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Cleaner Production
·
2022
·
IF 11.072
Xu-Hui Huang,Rong Liu,Jia-Nan Chen,Xiu-Ping Dong,Bei-Wei Zhu,Lei Qin
Erythromycin has caused severe risk on the aquatic ecological environment and food chain propagation due to its extensive use, environmental persistence, and low efficiency of sewage removal. Our study monitored the erythromycin accumulation, self-detoxification behavior, and metabolic response of turbot in erythromycin-contaminated environments using a multi-omics method with the aim to improve the safety of turbot consumption and provide guidance for turbot culture. Erythromycin was found to rapidly accumulated and reached its peak in turbot in the first two days of exposure to erythromycin. Residual levels in the gill, liver, kidney, muscle, and blood were detected at 95.19, 72.80, 68.74, 51.3, and 12.33 ng/g, respectively. The risk of erythromycin residues in turbot can be effectively reduced by a withdrawal period longer than 12 d. Erythromycin-contaminated turbot muscles showed a significant decrease in flavor properties, particularly umami, sweet, and aroma precursors. A total of 232 lipids in four main categories, glycerides, phospholipids , fatty acyl, and sphingolipids, were found to show significant changes in the turbot muscles. The interacting metabolic network formed by taurine, glycerophospholipid, arginine, alanine , and glutamate metabolism, wherein turbot responded to erythromycin was verified through 14 significantly altered metabolites. A withdrawal period of at least half a month and the corresponding product labels for antibiotics should be formulated before the fish exposed to antibiotics during intensive culture are sold to protect the interests of consumers. This study could serve as a reference for the quality and safety risk assessment of turbot and other highly economical fishes affected by antibiotics exposure.
研究领域:
环境科学
·
水产养殖学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
NATURE GENETICS
·
2016
·Vol. 49
·期 1
·119-124
·
IF 31.616
Shao Changwei,Bao Baolong,Xie Zhiyuan,Chen Xinye,Li Bo,Jia Xiaodong,Yao Qiulin,Ortí Guillermo,Li Wenhui,Li Xihong,Hamre Kristin,Xu Juan,Wang Lei,Chen Fangyuan,Tian Yongsheng,Schreiber Alex M,Wang Na,Wei Fen,Zhang Jilin,Dong Zhongdian,Gao Lei,Gai Junwei,Sakamoto Takashi,Mo Sudong,Chen Wenjun,Shi Qiong,Li Hui,Xiu Yunji,Li Yangzhen,Xu Wenteng,Shi Zhiyi,Zhang Guojie,Power Deborah M,Wang Qingyin,Schartl Manfred,Chen Songlin
Flatfish have the most extreme asymmetric body morphology of vertebrates. During metamorphosis, one eye migrates to the contralateral side of the skull, and this migration is accompanied by extensive craniofacial transformations and simultaneous development of lopsided body pigmentation1,2,3,4,5. The evolution of this developmental and physiological innovation remains enigmatic. Comparative genomics of two flatfish and transcriptomic analyses during metamorphosis point to a role for thyroid hormone and retinoic acid signaling, as well as phototransduction pathways. We demonstrate that retinoic acid is critical in establishing asymmetric pigmentation and, via cross-talk with thyroid hormones, in modulating eye migration. The unexpected expression of the visual opsins from the phototransduction pathway in the skin translates illumination differences and generates retinoic acid gradients that underlie the generation of asymmetry. Identifying the genetic underpinning of this unique developmental process answers long-standing questions about the evolutionary origin of asymmetry, but it also provides insight into the mechanisms that control body shape in vertebrates.
研究领域:
遗传学
·
发育生物学
·
比较基因组学
✓ 已验证
DOI
高影响因子
Food Bioscience
·
2022
·
IF 5.318
Fangchao Cui,Liqing Xi,Guiqin Zhao,Dangfeng Wang,Xiqian Tan,Jianrong Li,Tingting Li
Gout, as the second major metabolic disease after cardiovascular disease. Peptides have the advantages of natural and no side effects in the treatment and prevention of gout. This study aimed to screen xanthine oxidase (XOD) inhibitory peptides from Scophthalmus maximus protein as a drug basis for the treatment of gout. The specific results are as follows: Immobilization of xanthine oxidase 25.564 μg/mg by modified magnetic beads, and modified magnetic beads can be reused three times. Three decapeptides (FSLVHYAGTV, FTNEKLQQFF and WDDMEKIWHH) were screened, the IC 50 were 3.741, 4.046 and 2.203 mg/mL. Circular dichroism and three-dimensional fluorescence spectra showed that the inhibitory peptide would change the helical structure of XOD and the internal hydrophobic environment. Molecular docking showed that the inhibitory effect of inhibitory peptides depends on the number of unique amino acid residues in the active center of XOD enzyme . In conclusion, this study provides a method for screening anti-gout peptides, which not only solves the problem of treating gout, but also realizes the high-value utilization of marine products. In addition, this method has substantial application value because of its low cost and simple operation.
研究领域:
生物化学
·
药理学
·
海洋生物学
·
代谢疾病
✓ 已验证
DOI
高影响因子
FOOD CONTROL
·
2018
·
IF 3.667
Xinran Lv,Huanhuan Ma,Mengtong Sun,Yang Lin,Fengling Bai,Jianrong Li,Bolin Zhang
A novel bacteriocin DY4-2 was produced by strain DY4-2 which was isolated from cutlassfish ( Trichiurus lepturus ) and identified as Lactobacillus plantarum . This bacteriocin reached the maximum production at stationary phase after 24 h incubation. It was purified by ethyl acetate extraction followed by ultrafiltration, Sephadex G25 chromatography and high-performance liquid chromatography. The molecular mass of bacteriocin DY4-2 was determined as 1465 Da by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis. The amino acid sequence of bacteriocin DY4-2 was predicted to be HAQIGMTMNGSFR by MALDI-TOF-MS/MS. Bacteriocin DY4-2 was heat-stable (30 min at 121 °C) and kept active at the pH range of 2.5–5.5, but was sensitive to proteolytic enzymes. The mode of action of bacteriocin DY4-2 was bactericidal. It exhibited broad spectrum antimicrobial activity against fish pathogenic and spoilage bacteria, such as Pseudomonas fluorescens , P. aeruginosa , Vibrio parahaemolyticus , Aeromonas sobria and Listeria monocytogenes . The addition of partially purified bacteriocin DY4-2 in turbot fillets reduced the number of P. fluorescens by 2.7 log units at 4 °C storage for 12 days. Moreover, compared to the control, a significant decline in total viable counts and total volatile base nitrogen was observed in bacteriocin DY4-2 treated samples. Theses results suggested that bacteriocin DY4-2 had potential to act as seafood bio-preservative.
研究领域:
微生物学
·
食品科学
·
海洋生物学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Meng Dong,Lei Qin,Li-Xin Ma,Zi-Yuan Zhao,Ming Du,Konno Kunihiko,Bei-Wei Zhu
Nucleotide degradation in fish is an important biochemical change after death, which is closely related to freshness and sensory quality. However, except ATP-relative nucleotides, it remains unclear about changes in other nucleotide metabolites during postmortem stage. In this study, a strategy for the simultaneous quantification of 28 nucleobases, nucleosides, and nucleotides using hydrophilic interaction chromatography coupled with tandem mass spectrometry (HILIC-MS/MS) with positive/negative ion switching was developed. This method showed good linearity, precision, repeatability, and recovery. Furthermore, it was successfully applied to monitor the postmortem nucleotide degradation of turbot mince during chill (4 °C) and partial freezing (−3 °C) storage for 168 h. It was noted that the patterns of the changes in nucleotide metabolites differed considerably depending on the storage temperature. Meanwhile, the different pathway and speed of nucleotide catabolism in turbot mince was summarized based on the quantification data.
研究领域:
食品科学
·
生物化学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2011
·
IF 3.044
Rui Liu,Jixiang Chen,Kesheng Li,Xiaohua Zhang
A putative serine protease gene was cloned from the genomic DNA of Vibrio parahaemolyticus FYZ8621.4. The gene consisted of 1779 base pairs and encoded a 592 amino acid protein. The gene was expressed in Escherichia coli . The expressed protease was purified by Ni-NTA His-Bind Resin column and showed a 63 kDa band on SDS–PAGE. The protease exhibited proteolytic activity on gelatin agar plate and showed maximal proteolytic activity at pH 8.0 and 37 °C. It hydrolyzed N-α-benzoyl- l -tyrosine p-nitroanilide (BAPNA), but did not N-benzoyl- l -arginine ethylester (BAEE), N-benzoyl- l -tyrosine ethylester (BTEE) and N-acetyl- l -tyrosine ethylester (ATEE). Mutants at conserved residues Asp 51 (Asp 51 –Asn), His 89 (His 89 –Asp) and Ser 318 (Ser 318 –Leu, Ser 318 –Pro) lost proteolytic activities completely. The protein was confirmed to belong to serine protease. The purified serine protease was toxic to zebrafish with a LD 50 of 15.4 μg/fish. A DNA vaccine was constructed by inserting the mutated serine protease (Ser 318 –Pro) gene into pEGFP-N1 plasmid. The pEGFP-N1/m- vps was transfected in HeLa cells. The serine protease was confirmed to be expressed by fluorescence microscopy observation and Western blotting analysis. The pEGFP-N1/m- vps was further observed to express in muscle of the injected turbot ( Scophthalmus maximus ) by Western blotting seven days after immunization. Efficient protection against lethal V. parahaemolyticus challenge was observed on the vaccinated turbot with pEGFP-N1/m- vps , with the highest relative percent survival (RPS) of 96.11%. Significant specific antibody responses were also observed in the turbot vaccinated with the DNA vaccine. The results indicated that the serine protease might be a potential virulence factor and could be used as an efficient vaccine candidate for the disease control caused by V. parahaemolyticus .
研究领域:
微生物学
·
分子生物学
·
免疫学
·
水产养殖
·
疫苗学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AQUACULTURE
·
2024
·
IF 3.9
Yuqing Zhang,Wenying Zhang,Yanmin Jian,Shujing Zhang,Dongwu Liu,Kang Zheng,Xungang Tan,Feng You,Qiuxiang Pang,Shuang Jiao
Olive flounder ( Paralichthys olivaceus ) is a valuable mariculture fish that shows female-biased sexual size dimorphism (SSD), yet the molecular mechanisms responsible for this phenomenon remain poorly understood. In this study, the growth differences between females and males were compared, and comparative transcriptome analyses of brains/pituitaries, livers, muscles, and gonads from 13-month-old females and males were performed. The critical time window for SSD was from 13- to 14-month-old, with females reaching significantly larger sizes than males. Muscle cell size and plasma growth hormone (GH), insulin-like growth factor 1 and 2 (IGF1, IGF2), 11-ketotestosterone (11-KT), and 17β-estradiol (E2) levels had no significant differences between the sexes of 13- and 14-month-old flounders. Furthermore, the RNA-seq data revealed differential expression in the genes encoding the receptors for these hormones, suggesting that regulation may occur at the receptor level, potentially leading to SSD during the critical window. RNA-seq analysis identified 88, 645, 414, and 15,833 differentially expressed genes between females and males in brains/pituitaries, livers, muscles, and gonads, respectively. KEGG (Kyoto Encyclopedia of Genes and Genomes) and GSEA-based KEGG analyses revealed that some pathways associated with growth and development, including “prolactin signaling pathway” in brains/pituitaries, “cell cycle”, “oocyte meiosis”, and “DNA replication” in livers, “focal adhesion”, “ECM-receptor interaction”, “protein digestion and absorption”, “arginine and proline metabolism”, and “cysteine and methionine metabolism” in muscles, and “Hippo signaling pathway” and “JAK-STAT signaling pathway” in gonads may be involved in the establishment of sexual growth differences. Notably, females had lower steroid hormone synthesis abilities than males in brains/pituitaries, livers, and gonads, indicating the importance of sex steroid hormones in the establishment of SSD. Moreover, genes including frizzled3 ( fzd3 ) and prolactin ( prl ) in brains/pituitaries, and growth hormone receptor ( ghr ), estrogen receptor ( esr1 ), insulin-like growth factor binding protein-3 ( igfbp3 ), bone morphogenetic protein 8A-like ( bmp8a ), leptin receptor ( lepr ), and lipoprotein lipase-like ( lpl ) in livers emerge as potential candidates for the establishment of SSD. In conclusion, this study may help clarify the molecular mechanisms involved in SSD-related growth traits in flounder and provide basic data for genetic breeding of flounder.
研究领域:
海洋生物学
·
基因组学
·
转录组学
·
内分泌学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）