FOOD CHEMISTRY
·
2025
·
IF 9.8
Yan Tao,Chongying Liao,Fangli He,Yixuan He,Sihui Yang,Liu-Pan Yang,Li-Li Wang
Creatine (CR) is an active compound closely associated with meat quality. However, current techniques for measuring CR in meat face challenges in achieving rapid and low-cost detection. In this study, nanozyme based on iron-doped carbon dots (Fe-LCDs) was synthesized by employing sodium lignosulfonate and iron as the precursors. Iron incorporation was crucial for significantly enhancing the peroxidase-like activity of Fe-LCDs. A cascade sensing platform was developed by integrating creatinase (CRH) and sarcosine oxidase (SOx) with Fe-LCDs. In this system, CR interacts with the catalytic framework to generate superoxide anions, which catalyzes the oxidation of 3,3′,5,5’-Tetramethylbenzidine (TMB) to produce a blue-colored product, enabling colorimetric detection of CR. Furthermore, a sensing platform compatible with smartphones was established, successfully facilitating rapid, sensitive, and visual detection of CR in meat. This research not only synthesized an effective CDs-based nanozyme but also devised a novel and promising nanoparticle-based sensing platform for CR detection.
研究领域:
食品科学
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生物化学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Jingfang Guo,Feng Zhao,Zhenhui Yue,Zhen Lei
Rapid and accurate detection of carbosulfan residues in vegetables is important for ensuring food safety. Herein, based on the unique hydrolysis behavior of carbosulfan, a nanozyme-based colorimetric sensing platform was proposed for detection of carbosulfan. Hollow polydopamine/MnO 2 nanoparticles (H-PDA/MnO 2 NPs) with excellent oxidase-like activity were synthesized, which can promote the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) into blue oxidative product (oxTMB). Under acidic conditions, carbosulfan can be decomposed and produce reductive sulfide species (-SH), which are capable to disintegrate MnO 2 NPs, resulting in decreased oxidase-like activity of H-PDA/MnO 2 NPs. Based on the inhibitory effect on oxidase activity of H-PDA/MnO 2 NPs, an acetylcholinesterase-free colorimetric assay was proposed for detection of carbosulfan with low limit of detection of 0.63 ng mL −1 . Integrating test swabs with smartphone, a portable colorimetric sensor was constructed, showing great potential for on-site detection. To demonstrate the feasibility of this method, carbosulfan in real vegetable samples were determined.
研究领域:
食品安全
·
分析化学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Shuyun Zhu,Cuihua Lei,Jing Sun,Xian-En Zhao,Xiao Wang,Xiaolu Yan,Wei Liu,Hua Wang
In the present study, a novel colorimetric sensing platform was constructed for probing the NAD + /NADH-dependent biocatalytic transformations. Manganese dioxide (MnO 2 ) nanosheets as an oxidase-mimicking nanomaterial could directly oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) into oxTMB without the need of H 2 O 2 . Importantly, it was found that NADH could easily trigger the decomposition of MnO 2 nanosheets, causing the decrease of solution absorbance. This is demonstrated by the enzyme catalysis reactions of two dehydrogenase models, ethanol dehydrogenase (ADH) and lactate dehydrogenase (LDH), where NADH is formed and consumed, respectively, in their physiological enzymatic reactions. A MnO 2 -TMB sensing platform was thereby proposed for the analysis of their catalytic reaction substrates including ethanol and pyruvic acid. The detection limits of ethanol and pyruvic acid can reach 5.0 μM and 100 nM, respectively. The developed optical biosensing platform can be used to develop other dehydrogenase-based biosensors followed by monitoring their substrates.
研究领域:
生物化学
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纳米生物学
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分析化学
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产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 412
·期 4
·963-972
·
IF 3.286
Zhuo Shujuan,Fang Jing,Zhu Changqing,Du Jinyan
In this study, palladium/carbon dot composites (Pd-CDs) were fabricated via a facial hydrothermal route using ethanediamine and palladium chloride dihydrate as precursors. The obtained Pd-CDs showed an excellent intrinsic peroxidase-like activity, which could catalyze the oxidization of 3,3′5,5′-tetramethylbenzidine with the assistance of hydrogen peroxide (H 2 O 2 ) and thus resulted in color change, accompanied by an absorption peak which appeared at 652 nm. Such response is H 2 O 2 concentration–dependent and allows for the assay of H 2 O 2 in the range of 0.1 to 30 μM with a limit of detection of 0.03 μM. Simultaneously, by combination of enzymatic oxidation of glucose with glucose oxidase and Pd-CD catalytic reaction, a colorimetric sensing platform was also constructed for glucose detection with high selectivity and sensitivity (limit of detection as low as 0.2 μM). Additionally, the proposed method exhibited capability for determination of glucose in real samples (fruit juice) with satisfactory recovery (98.5–103%), indicating potential application prospects in biochemical analysis.
研究领域:
生物化学
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分析化学
·
纳米材料科学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Jinjie Hou,Pei Jia,Kairong Yang,Tong Bu,Xinyu Sun,Li Wang
In the last decades, nanozymes have drawn significant attention because of their higher stability and lower preparation cost than natural enzymes. In this report, the novel Ru@V 2 O 4 nanocomposite with multi-enzyme-like activities has been synthesized via one-step hydrothermal method and simple stirring. It was proved that the prepared Ru@V 2 O 4 nanowires presented magnificent tetra-enzyme-like activities, comprising oxidase-like (OD-like), peroxidase-like (POD-like), catalase-like (CAT-like) and superoxide dismutase-like (SOD-like) activity. Firstly, the kinetic investigation validated the OD and POD-mimics behaviors of Ru@V 2 O 4 nanowires, which were in conformity with the Michaelis-Menten kinetics. Then a bifunctional colorimetric sensing platform was constructed for the sensitive detection of H 2 O 2 and cysteine (Cys) on the basis of POD-like and OD-like enzyme activities of Ru@V 2 O 4 nanowires. H 2 O 2 could be detected by Ru@V 2 O 4 nanowires over a range of 1–500 μM and the response limit was 0.788 μM. Under optimal conditions, the degree of discoloration was linearly proportional to the concentration of Cys in the range of 3–50 μM with a low detect limit of 0.139 μM. Besides, Ru@V 2 O 4 nanowires can detect H 2 O 2 in water samples and Cys in serum samples. Therefore, this research significantly expands the application of the tetra-enzyme mimetic Ru@V 2 O 4 nanowires in the fields of biosensing, biocatalysis, environmental monitoring and medical diagnosis.
研究领域:
生物传感
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纳米材料
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医学诊断
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环境监测
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产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2022
·
IF 7.968
Yan Xue,Hongyu Li,Tao Wu,Hui Zhao,Yan Gao,Xixi Zhu,Qingyun Liu
Detecting of sulfur ions (S 2- ) in environment is critical to human health. In this work, a fast and convenient colorimetric sensing platform for S 2- was developed. In detail, Pt deposited on sea urchin-like CuCo 2 O 4 nanostructures (Pt/CuCo 2 O 4 ) was synthesized and demonstrated to possess enhanced peroxidase-like performance, similar to the natural horseradish peroxidase (HRP). Pt/CuCo 2 O 4 can catalyze colorless 3, 3, 5, 5-Tetramethylbenzidine (TMB) to be oxidized by H 2 O 2 into a blue product (oxTMB). When the above system encounters sulfur ions (S 2- ), the peroxidase-like activity of Pt/CuCo 2 O 4 is inhibited, accompanied with the color change of reaction solutions. On this basis, a colorimetric sensing platform for S 2- can be established. Kinetic experiments verified Pt/CuCo 2 O 4 possessed a higher affinity with substrate than that of HPR. The excellent peroxidase-like performance of Pt/CuCo 2 O 4 was attributed to two active species including hydroxyl radical (•OH) and superoxide radical (•O 2 - ) in the catalytic process. The linear range of the proposed sensing platform for S 2- was 0.5–10 μM as well as the low limit of detection is 85.9 nM, much lower than the World Health Organization regulations. The sensing platform had been successfully applied to the detection of S 2- in tap water and lake water, which provided a convenient method for detecting S 2- in wastewater.
研究领域:
材料科学
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环境科学
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生物化学
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产品关联
DOI
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2022
·Vol. 38
·期 4
·919-927
·
IF 2.726
Zhu Junlun,Cui Qian,Wen Wei,Zhang Xiuhua,Wang Shengfu
Although great progress has been made in the advancement of nanozymes, most of the studies focus on mimicking peroxidase, oxidase, and catalase, while relatively few studies are used to mimic laccase. However, the use of nanomaterials to mimic laccase activity will have great potential in environmental and industrial catalysis. Herein, Cu/CuO-graphene foam with laccase-like activity was designed for the identification of phenolic compounds and the detection of epinephrine. In a typical experiment, the formation mechanism of Cu/CuO-graphene foam was investigated during the pyrolysis process by thermogravimetric-mass spectrometry. As a laccase mimic, Cu/CuO-graphene foam exhibited excellent catalytic activity with a Michaelis-Menten constant and a maximum initial velocity of 0.17 mmol/L and 0.012 µmol·L −1 ·s −1 , respectively. Based on this principle, Cu/CuO-graphene foam nanozyme could differentially catalyze phenolic compounds and 4-aminoantipyrine for simultaneous identification of phenolic compounds. Furthermore, a colorimetric sensing platform was fabricated for the quantitative determination of epinephrine, showing linear responses to epinephrine in the range of 3 µg/mL to 20 µg/mL with the detection limit of 0.2 µg/mL. The proposed Cu/CuO-graphene foam nanozyme could be applied for the identification of phenolic compounds and the detection of epinephrine, showing great potential applications for environmental monitoring, biomedical sensing, and food detection fields.
研究领域:
材料科学
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环境科学
·
分析化学
·
生物技术
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Jingjing Li,Zhixue Zhu,Manru Zhang,Qingxin Zhang,Wenyu Sun,Yeru Wang,Long Jiang,Zhiqiang Guo,Yu Wang,Su Liu,Jiadong Huang,Jinghua Yu
Uracil-DNA glycosylase (UDG) is a common glycosylase that can expressly recognize and remove damaged uracil bases, and the ultrasensitive detection of which is significant to maintain genomic stability and early clinical diagnosis of disease. Herein, we proposed a sensitive colorimetric sensing platform to detect UDG. Combined with target-manipulated drawstring DNAzyme and [email protected] nanorods ( [email protected] NRs) indicator, we achieved in naked-eyes observation and ultrasensitive detection of UDG. Briefly, when the UDG exists, the dynamic reaction of rope pulling will occur generating the active conformation of DNAzyme. The cutting effect will be further produced when we add Mg 2+ , thus the generated trigger chain can mediate the occurrence of CHA reaction, followed by generating amount of ·OH which can etch [email protected] NRs causing the shifted of localized surface plasmon resonance (LSPR) peak. By contrast, there is no obvious shift of LSPR peak. This strategy shows extraordinary specificity and sensitivity toward UDG providing a detection limit of 4.6 × 10 −5 U mL −1 . By using of this method, we detected UDG specifically in complex samples, proving that it’s potential applications in biomedical research and clinical diagnosis are fantastic.
研究领域:
生物化学
·
纳米技术
·
生物医学工程
·
临床诊断
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 8
·1-11
·
IF 5.3
Wang Chunxin,Deng Rong,Li Haiqin,Liu Zhigang,Niu Xiaofeng,Li Xiaochun
An intelligent colorimetric sensing platform integrated with in situ immunomagnetic separation function was developed for ultrasensitive detection of Escherichia coli O157: H7 ( E. coli O157: H7) in food. Captured antibody modified magnetic nanoparticles (cMNPs) and detection antibody/horseradish peroxidase (HRP) co-functionalized AuNPs (dHAuNPs) were firstly synthesized for targeted enrichment and colorimetric assay of E. coli O157: H7, in which remarkable signal amplification was realized by loading large amounts of HRP on the surface of AuNPs. Coupling with the optical collimation attachments and embedded magnetic separation module, a highly integrated optical device was constructed, by which in situ magnetic separation and high-quality imaging of 96-well microplates containing E. coli O157: H7 was achieved with a smartphone. The concentration of E. coli O157: H7 could be achieved in one-step by performing digital image colorimetric analysis of the obtained image with a custom-designed app. This biosensor possesses high sensitivity (1.63 CFU/mL), short detecting time (3 h), and good anti-interference performance even in real-sample testing. Overall, the developed method is expected to be a novel field detection platform for foodborne pathogens in water and food as well as for the diagnosis of infections due to its portability, ease of operation, and high feasibility. Graphical Abstract
研究领域:
生物技术
·
分析化学
·
食品科学
·
微生物学
·
传感器与执行器
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 58
·33589-33595
·
IF 3.049
Yun Zhang,Shuyou Shi,Jiajia Xing,Wenqing Tan,Chenguang Zhang,Lin Zhang,Huan Yuan,Miaomiao Zhang,Jinjuan Qiao
In this study, a novel colorimetric sensing platform was developed for the detection of S. aureus using dog immunoglobulin G (IgG) as the capture antibody and chicken anti-protein A immunoglobulin Y labeled with horseradish peroxidase (HRP-IgY) as the detection antibody. Dog IgG labeled with magnetic beads was used to capture S. aureus through the interaction between the Fc region of dog IgG and Staphylococcal protein A (SPA). HRP-IgY was introduced to recognize the residual SPA on the surface of S. aureus and to create a sandwich format, after which a soluble 3,3′,5,5′-tetramethylbenzidine (TMB) substrate was added. A stop solution was utilized to cease the enzymatic chromogenic reaction, and then optical density was read at 450 nm. Under optimal conditions, the proposed method displayed a low detection limit of 1.0 × 103 CFU mL−1 and a wide linear range of 3.1 × 103 to 2.0 × 105 CFU mL−1. This detection method exhibited high specificity against other foodborne bacteria. The recovery rates ranged from 95.2% to 129.2%. To our knowledge, this is the first report to employ dog IgG and chicken IgY as an antibody pair to detect S. aureus. This technique exhibits high application potential for S. aureus monitoring in various kinds of samples.
研究领域:
生物传感器
·
微生物学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）