ANALYTICAL CHEMISTRY
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2018
·
IF 6.042
Jiahui Zhao,Shuang Wang,Shasha Lu,Xingfu Bao,Jian Sun,Xiurong Yang
An enzyme cascade-triggered reaction with novel signal generation mechanism is beneficial for the development and insight of the enzyme cascade, which is extensively used for signal transduction in potential applications. Inspired by the fluorogenic and chromogenic reaction between dopamine and resorcinol, and the specific catalytic properties of alkaline phosphatase (ALP) and tyrosinase, we designed and synthesized an unconventional substrate of ALP, named p-aminoethyl-phenyl phosphate disodium salt (PAPP). As expected, the ALP and tyrosinase-incubated PAPP solution exhibited pale yellow with intense blue fluorescence upon addition of resorcinol, owing to the ALP-catalyzed transformation of PAPP into an intermediate tyramine, and the tyrosinase-catalyzed hydroxylation of tyramine to dopamine, as well as the specific reaction between dopamine and resorcinol. Therefore, an enzyme cascade system has been developed herein based on the ALP and tyrosinase coupled enzymes-triggered fluorogenic and chromogenic reaction. According to the direct relationship between the activity of ALP/tyrosinase and absorbance/fluorescence intensity of the resultant solution, the proposed enzyme cascade-triggered reaction was utilized for assaying ALP and tyrosinase activity with fluorometric and colorimetric dual-readout signals. Furthermore, such enzyme cascade catalysis process was integrated into the ALP-based cascade enzyme-linked immunosorbent assay with dual-readout signals, resulting in the sensitive detection of cardiac troponin I in diluted serum.
研究领域:
生物化学
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医学检测
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免疫分析
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DOI
PubMed
高影响因子
Coatings
·
2021
·Vol. 12
·期 1
·38
·
IF 2.881
Jingyang Zhu,Lifeng Yin,Weiyi Zhang,Meilian Chen,Dongsheng Feng,Yong Zhao,Yongheng Zhu
Deltamethrin (DEL) is one of the most commonly used pyrethroid pesticides that can cause serious harms to the ecological environment and human health. Herein, we have developed a paper-based colorimetric sensor impregnated with gold nanoparticles (AuNPs) for on-site determination of DEL pesticide. AuNPs show obvious color change on paper device with the presence of DEL. Measuring the gray intensity of the AuNPs on the reaction zone of the paper sensor allows accurate quantitative analysis. The detection mechanism of DEL on paper sensor was confirmed by UV-Vis spectrophotometry (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), and transmission electron microscope (TEM). Under optimal conditions, the colorimetric sensor exhibited high sensitivity, rapid detection, and low detection limit within the values stipulated by Chinese detection standards (LOD = 0.584 mg/L). Besides, detecting DEL in vegetable and fruit samples also gave satisfying results, which were much consistent with those obtained by spectrophotometry. Overall, this work provided a user-friendly, cost-effective and visualized detection platform, which could be applied to rapidly detect DEL pesticides in the food safety field.Keywords:colorimetric signal;deltamethrin pesticide;gold nanoparticles;paper sensor;rapid detection
研究领域:
食品安全
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环境科学
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分析化学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2022
·
IF 9.221
Tianshi Zhang,Xiaoyun Hu,Baiyi Zu,Xincun Dou
In solid targets analyzing, independent sampling pretreatment steps are usually necessary to ensure the sensitivity and robustness. Although integrated devices provide one-step sampling-analysis strategy, the enhancement of the promotion of the safe sampling and ultrasensitive sensing still needs deep-going investigation. Here, a bilayer hydrogel device was designed to realize on-site sampling and reaction with the restriction of the colorimetric signal dispersion by constructing asymmetrical diffusion. The restricted diffusion tendency of the reaction product in the bilayer hydrogel system was verified by mapping the reaction distribution. The detection of nitrite was realized through the bilayer hydrogel device with a good linear relationship towards solid nitrite residues in a range of 0–20 nmol and a naked-eye detection limit of 2.898 ng (0.042 nmol). The applicability in the analysis of practical scenarios was demonstrated by the well-sampling of the nitrite residues on different surfaces and the retention of the pattern shapes. A portable colorimetric detector incorporated with this hydrogel device was explored and showed a promising utilization for the on-site detection of nitrite residues on trolley case, bag and pickles. We expect that this bilayer hydrogel signal enhancing strategy will be favorable for the ultrasensitive probe-based analyses and integrated sensing device investigations.
研究领域:
分析化学
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生物传感
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环境监测
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DOI
高影响因子
FOOD CHEMISTRY
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2022
·
IF 9.231
Yuanyuan Jiang,Yanrong Miao,Zhenyu Ding,Yizhong Lu
Chlorpyrifos is widely used in agriculture but their residue is a threat to food safety and human health. The study aims to find an accurate and sensitive detection method for chlorpyrifos. Silicon-based nanoparticles (Si BNPs) with fluorescent and colorimetric dual signals are in-situ formed through the alkaline phosphatase (ALP) triggered reaction. The dual-mode sensor for chlorpyrifos can be facilely fabricated because the existence of chlorpyrifos would inhibit the activity of ALP and thus affect the production of Si BNPs. Under optimal condition, the fluorescence intensity and absorption intensity linearly correlate with the logarithm of chlorpyrifos concentration over wide range of 0.5–5000 ng/mL and 1–5000 ng/mL with low detection limit by the two detection modes, respectively. The dual-mode sensor is further applied to the detection of spiked chlorpyrifos in practical samples with satisfactory recovery, rendering it as a promising candidate to current methodologies for the simple and accurate detection of chlorpyrifos.
研究领域:
分析化学
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食品安全
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环境科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2018
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IF 4.946
Wan Huang,Hongyu Zhang,Guosong Lai,Shun Liu,Bo Li,Aimin Yu
By combination of the aptamer biorecognition with the colorimetric signal transduction of a DNAzyme-functionalized nanoprobe, a new biosensing method was developed for the rapid and sensitive detection of chloramphenicol (CAP). The nanoprobe was prepared through the functionalization of gold nanoparticles with the complementary oligonucleotide against aptamer and high-content hemin/G-quadruplex DNAzyme. When one-step incubating the nanoprobe and CAP at a constructed aptamer-magnetic bead (MB) biosensing platform, due to the competitive biorecognition reaction, the nanoprobes related with CAP amounts were quantitative captured onto the MB surface. Based on the catalytic reaction of the peroxidase-mimicking DNAzyme, a colored substance was produced for the colorimetric signal transduction of the method. Due to the great signal amplification of the nanoprobe, a very low detection limit down to 0.13 pg/mL was obtained. Considering the excellent performance of the aptasensing method and satisfactory results for milk sample experiments, it indicates good reliability for practical applications.
研究领域:
分析化学
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生物传感器
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食品安全
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纳米技术
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Chen Wang,Chengyan Xiang,Haoran Zhang,Gao Zhang,Qi Zhang,Peiwu Li,Xiaoqian Tang
Dibutyl phthalate (DBP), a priority pollutant among phthalic acid esters (PAEs) exhibits significant reproductive and respiratory toxicity. In this study, a multifunctional metal-organic frameworks-mediated colorimetric/photothermal immunosensor was established for the quantitative detection of DBP. Firstly, a highly sensitive and specific monoclonal antibody (mAb), designated 3A5, was prepared with a sensitivity IC 50 value of 16.29 ng/mL. Secondly, a metal-organic framework material (ZrO₂@C) was synthesized via a two-step pyrolysis process of UiO-66. Subsequently, a multifunctional immunoprobe was prepared for the detection of DBP. Using ZrO₂@C-based colorimetric and photothermal dual-signal immunosensor ensured the accuracy of the detection results, as the multiple of these signals effectively guaranteed the reliability of the results. The limit of detection (LOD) for the dual signal was 0.766 ng/mL (colorimetric signal) and 0.465 ng/mL (photothermal signal). In conclusion, this work presented a novel and feasible approach for the development of multifunctional nanomaterials for the fabrication of immunosensors.
研究领域:
环境科学
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分析化学
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免疫学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Shu Zhu,Chonghui Wei,Yuxiang Xia,Kewei Yu,Meiyun Chen,Yujie Zhao,Xingyu Luo
Uric acid (UA) is an important biomarker for assessing human health. The abnormal contents of UA in biological fluids are closely involved with many diseases. Although conventional methods for UA analysis with satisfied detection performances have been well-designed, they are still restricted by bulky instrument requirements and insufficiency during the detection processes. Herein, we have built a cellulose paper-based nanozyme detection platform (CF-cPADs) by immobilizing the cobalt-iron layered double hydroxide (CoFe LDH) for point-of-care (POC) detection of UA. In this assay, CoFe LDH with peroxidase (POD)-like activity has been synthesized to be immobilized onto the cellulose paper, and then the uricase (Uox) and CoFe LDH involved cascaded enzymatic reaction has been designed to realize the conversion from UA contents to colorimetric signals, which can be further analyzed by smartphone. The highly sensitive, stable, and reproducible CF-cPADs exhibit a wide linear range varied from 1 to 100 μM with a comparable limit of detection (LOD) as low as 0.92 μM for satisfying UA analysis in real samples. This approach can provide new insights into the development of cost-effective, portable, rapid, easy-to-use, and on-site detection of biomarkers. It will overcome the drawbacks in clinical diagnosis which are highly dependent on the professionals and analytical instruments.
研究领域:
生物医学工程
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分析化学
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即时诊断
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产品关联
DOI
高影响因子
ANALYTICAL SCIENCES
·
2024
·Vol. 40
·期 8
·1459-1473
·
IF 1.8
Xu Xin,Zhang Ze,Shen Tong,Pan Hongzhi,Chang Dong
The main reason for the high mortality rate of non-small cell lung cancer is that patients are usually diagnosed at an advanced stage of the disease. Exosomes, small membrane vesicles secreted by normal cells or tumor cells, play a significant role in the progression of NSCLC. This study successfully optimized the preparation of artificial nanoenzymes self-coupling with horseradish peroxidase (IrO 2 NPs@HRP-AptCD63), without adding any ligand, demonstrating remarkable catalytic activity suitable for detecting the EGFR protein on the surface of NSCLC exosomes. When fused with the CD63 aptamer for identifying NSCLC exosomes, IrO 2 NPs@HRP showed enhanced catalytic activity in the 3,3′,5,5′-tetramethylbenzidine-H 2 O 2 oxidation–reduction system, thereby enhancing the colorimetric signal. This phenomenon can be distinguished by the naked eye and quantified using a UV–Vis spectrophotometer. Meanwhile, as the redox reaction occurs, the current signal of 3,3′,5,5′-tetramethylbenzidine-H 2 O 2 , acting as an electrolyte, changes. The developed aptasensor generates dual-mode signal outputs, firstly, to visually assess the samples for their positive or negative status, and subsequently employ more in-depth electrochemical or colorimetric analysis methods for a detailed quantitative analysis of suspected positive samples. The detection limits of electrochemical analysis and colorimetric analysis were 0.9 × 10 3 particles/mL and 0.14 × 10 3 particles/mL, respectively. Compared with traditional biomarkers such as CA125, this method exhibits exceptional specificity, capable of simultaneously distinguishing serum exosomes of healthy volunteers, COPD patients, and NSCLC patients, promoting exosome detection in mouse models for tumor monitoring. Additionally, it elucidates the changes in EGFR protein expression on the surface of serum exosomes throughout the developmental trajectory. Graphical abstract
研究领域:
癌症生物学
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纳米技术
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生物传感器
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分子诊断
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Nannan Li,Fan Yang,Liping Li,Ruiping Zhang
The utilization of artificial multienzyme-catalyzed cascade reactions have greatly benefited biosensors. This study presents a novel nanoreactor consisting of manganese ions-doped hollow carbon nanospheres (Mn@HCNs), which can rapidly transform molecular information into an easily comprehensible colorimetric signal. The Mn@HCNs nanoreactor was utilized to detect acid phosphatase (ACP) within a range of 0.5–10 mU/mL, achieving a limit of detection (LOD) as low as 0.0851 mU/mL. The superiority of our nanoreactor over traditional ACP detection methods stems from its exceptional substrate affinity and the multi-enzymatic behaviors of Mn@HCNs. Especially, the specific POD-like activity (SA) value of Mn@HCNs was superior to most reported Fe 3 O 4 and other single-atom enzymes. Furthermore, this nanoreactor exhibited exceptional selectivity against protein and ion interference, and was furthered to detect ACP in clinical serum samples. The work may extend the development of artificial multienzyme-catalyzed cascade reactions for molecular biosensors and clinical application.
研究领域:
纳米技术
·
生物化学
·
分析化学
·
生物医学工程
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产品关联
DOI
高影响因子
ANALYST
·
2023
·Vol. 148
·期 15
·3632-3640
·
IF 4.2
Xinyue Yuwen,Yingzhao Zeng,Shilong Ruan,Xin Li,Guosong Lai
The plasmonic colorimetric biosensors are very favorable for the on-site testing and naked-eye screening of analytes from real samples, but how to realize their highly sensitive assays with simple manipulations is still a great challenge. Herein we designed a target-triggered dual cascade nucleic acid recycling strategy to amplify the assembly of a hyperbranched DNA nanostructure and thus developed a novel kanamycin colorimetric biosensing method. The first cycle arising from the aptamer recognition-triggered strand displacement reaction and its cascade cycle constructed on the catalytic reaction of two nucleases could release an output DNA to trigger the assembly of the DNA nanostructure. Based on the high capture of alkaline phosphatase at this DNA nanostructure to induce the localized surface plasmon resonance change of gold nanobipyramids (Au NBPs), an ultrasensitive colorimetric signal transduction strategy was constructed. Through the measurement of the shift of the characteristic absorption wavelength of Au NBPs, a very wide linear range from 10 fg mL−1 to 1 ng mL−1 and a very low detection limit of 1.4 fg mL−1 were obtained. Meanwhile, the obvious multicolor change of Au NBPs could be used for the visual semi-quantitative analysis of Kana residues. The whole homogeneous assay process well simplified the manipulation and also ensured the excellent repeatability. These excellent performances determine the great potential of the method for future applications.
研究领域:
生物传感
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纳米技术
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分析化学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2022
·
IF 12.545
Xiangqin Meng,Sijia Zou,Dandan Li,Jiuyang He,Ling Fang,Haojue Wang,Xiyun Yan,Demin Duan,Lizeng Gao
The coronavirus disease 2019 (COVID-19) pandemic has created a huge demand for sensitive and rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The current gold standard for SARS-CoV-2 detection is reverse transcription-polymerase chain reaction (RT-PCR)-based nucleic acid amplification. However, RT-PCR is time consuming and requires specialists and large instruments that are unattainable for point-of-care testing (POCT). To develop POCT for SARS-CoV-2, we combined recombinase polymerase amplification (RPA) and FeS 2 nanozyme strips to achieve facile nucleic acid amplification and subsequent colorimetric signal enhancement based on the high peroxidase-like activity of the FeS 2 nanozymes. This method showed a nucleic acid limit of detection (LOD) for SARS-CoV-2 of 200 copies/mL, close to that of RT-PCR. The unique catalytic properties of the FeS 2 nanozymes enabled the nanozyme-strip to amplify colorimetric signals via the nontoxic 3,3′,5,5′-tetramethylbenzidine (TMB) substrate. Importantly, the detection of clinical samples of human papilloma virus type 16 (HPV-16) showed 100% agreement with previous RT-PCR results, highlighting the versatility and reliability of this method. Our findings suggest that nanozyme-based nucleic acid detection has great potential in the development of POCT diagnosis for COVID-19 and other viral infections.
研究领域:
分子生物学
·
病毒学
·
即时诊断
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Qian Zhao,Xinhui Xie,Liye Chen,Jiahao Wu,Ziping Jiang,Xingbo Shi
Aflatoxin B1 (AFB1), one of the most toxic mycotoxins, is commonly found in various food. This study develops a dual-mode biosensor integrating smartphone analysis and dark-field microscopy (DFM) observation for AFB1 detection based on the disintegration of core-shell Ag@Au nanoparticles (Ag@Au NPs). Employing a sandwich assay format, AFB1 aptamer assembled Ag@Au NPs are anchored on 96-well plate and disintegrated by nitric acid. Thereafter, the Ag core is etched, releasing Ag + that serve as colorimetric signal regulators upon the introduction of the glutathione-manganese dioxide nanosheets-3,3′,5,5′-tetramethylbenzidine substrate. The color signal is analyzed using a smartphone based on red-green-blue value analysis. Furthermore, the collapsed Au shell releases individual Au NPs that serve as dark-field scattering nanoprobes, producing a high-intensity green dark-field scattering signal under DFM observation. The detection limit (3.67 fg/mL) is 199/8174/2180-fold lower than those of colorimetric signal, commercial enzyme-linked immunosorbent assay kit, and high-performance liquid chromatography, respectively. Overall, smartphone analysis enables on-site preliminary screening without sophisticated instrumentation. DFM observation allows the ultra-sensitive quantitative detection in a laboratory. The proposed dual-mode biosensor will not only reduce false-positive/negative rate through the bidirectional signal readout, but also offer a practical, stable, and ultrasensitive strategy for AFB1 detection in food.
研究领域:
食品科学
·
分析化学
·
纳米技术
·
生物技术
·
显微镜技术
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）